{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T02:39:24Z","timestamp":1794278364652,"version":"4.9.0"},"reference-count":48,"publisher":"Baishideng Publishing Group Inc.","issue":"40","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Gastroenterol"],"abstract":"<jats:p>BACKGROUND<\/jats:p>\n                  <jats:p>Gut microbiota dysbiosis plays a central role in the pathogenesis of functional constipation (FC), but clinical treatment has shown uneven efficacy through methods of regulating intestinal flora. Most existing studies have concentrated on overall case-control comparisons, with limited stratification by age or constipation subtype. Knowledge of how these factors shape gut microbiota composition remains inadequate, constraining the development of effective personalized microbiota-based interventions.<\/jats:p>\n                  <jats:p>AIM<\/jats:p>\n                  <jats:p>To characterize gut microbiota profiles by age and constipation subtype and identify factors associated with microbial composition in FC.<\/jats:p>\n                  <jats:p>METHODS<\/jats:p>\n                  <jats:p>Ninety-two patients with FC completed questionnaires and underwent anorectal manometry and gastrointestinal transit tests; stool samples were collected for 16S rRNA gene sequencing. Stool samples alone were collected from 34 healthy controls. Patients with FC were categorized into age groups (young, middle-aged, and older) and classified into normal-transit constipation, slow-transit constipation (STC), defecatory disorder (DD), and mixed constipation subtypes. Gut microbial compositions across age groups and constipation subtypes were compared, and their correlations with clinical parameters were investigated.<\/jats:p>\n                  <jats:p>RESULTS<\/jats:p>\n                  <jats:p>The young group demonstrated significantly higher anal resting and squeeze pressures than the middle-aged and the older groups. Microbial richness and diversity were substantially lower in the older group than in middle-aged and young groups. Gut microbiota in the young group was predominantly enriched in taxa increasing sphincter tone and inhibiting intestinal peristalsis; the older group featured abundances of short-chain fatty acid-producing, beneficial taxa. The middle-aged group showed an enrichment of pro-inflammatory and pathogenic bacteria. Microbial richness and diversity were higher in STC than in the DD group. Moreover, STC group was enriched in taxa associated with slower peristalsis; DD group showed enrichment of motility-promoting taxa.<\/jats:p>\n                  <jats:p>CONCLUSION<\/jats:p>\n                  <jats:p>Significant differences in microbial composition and function were observed across age groups and constipation subtypes in FC, suggesting underlying pathophysiological heterogeneity and providing a basis for precision diagnosis and treatment.<\/jats:p>","DOI":"10.3748\/wjg.v31.i40.112637","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:54:06Z","timestamp":1761544446000},"source":"Crossref","is-referenced-by-count":8,"title":["Comprehensive microbial and clinical profiling of functional constipation: A stratified comparative study of age and constipation subtype"],"prefix":"10.3748","volume":"31","author":[{"given":"Hui-Ting","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Fen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju-Fang","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Yu","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Li","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2060","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(21)00111-4"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(19)30297-3"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/974573"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.12.034"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v16.i5.776"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1093\/gastro\/goab035"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00082.2014"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5560310"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00175.2021"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000002028"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2023.2197837"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-03152-2"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-016-0708-5"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1038\/nature25973"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-013-9488-5"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2024.1393596"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.14977"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-020-06109-5"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1186\/s12887-024-05383-w"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00209.2017"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cger.2013.10.003"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12144"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00261-19"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2016.1256525"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3RI0620-405RR"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms6040098"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13631"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00363"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.3390\/nu16183209"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1159\/000360010"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8040573"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2019-0283"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.3390\/nu16091289"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-5-23"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.202100408"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-65481-8_8"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2023.69.10.17"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2022-328389"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13095"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-025-03774-6"},{"key":"B41","unstructured":"Fallingborg J. 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