{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T06:36:34Z","timestamp":1778049394659,"version":"3.51.4"},"reference-count":73,"publisher":"Baishideng Publishing Group Inc.","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Gastroenterol"],"abstract":"<jats:p>BACKGROUND<\/jats:p>\n          <jats:p>External factors in ulcerative colitis (UC) exacerbate colonic epithelial permeability and inflammatory responses. Keratin 1 (KRT1) is crucial in regulating these alterations, but its specific role in the progression of UC remains to be fully elucidated.<\/jats:p>\n          <jats:p>AIM<\/jats:p>\n          <jats:p>To explore the role and mechanisms of KRT1 in the regulation of colonic epithelial permeability and inflammation in UC.<\/jats:p>\n          <jats:p>METHODS<\/jats:p>\n          <jats:p>A KRT1 antibody concentration gradient test, along with a dextran sulfate sodium (DSS)-induced animal model, was implemented to investigate the role of KRT1 in modulating the activation of the kallikrein kinin system (KKS) and the cleavage of bradykinin (BK)\/high molecular weight kininogen (HK) in UC.<\/jats:p>\n          <jats:p>RESULTS<\/jats:p>\n          <jats:p>Treatment with KRT1 antibody in Caco-2 cells suppressed cell proliferation, induced apoptosis, reduced HK expression, and increased BK expression. It further downregulated intestinal barrier proteins, including occludin, zonula occludens-1, and claudin, and negatively impacted the coagulation factor XII. These changes led to enhanced activation of BK and HK cleavage, thereby intensifying KKS-mediated inflammation in UC. In the DSS-induced mouse model, administration of KRT1 antibody mitigated colonic injury, increased colon length, alleviated weight loss, and suppressed inflammatory cytokines such as interleukin (IL)-1, IL-6, tumor necrosis factor-\u03b1. It also facilitated repair of the intestinal barrier, reducing DSS-induced injury.<\/jats:p>\n          <jats:p>CONCLUSION<\/jats:p>\n          <jats:p>KRT1 inhibits BK expression, suppresses inflammatory cytokines, and enhances markers of intestinal barrier function, thus ameliorating colonic damage and maintaining barrier integrity. KRT1 is a viable therapeutic target for UC.<\/jats:p>","DOI":"10.3748\/wjg.v31.i6.102070","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T01:47:31Z","timestamp":1736473651000},"source":"Crossref","is-referenced-by-count":5,"title":["Keratin 1 modulates intestinal barrier and immune response <i>via<\/i> kallikrein kinin system in ulcerative colitis"],"prefix":"10.3748","volume":"31","author":[{"given":"Xiang-Qian","family":"Dong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Hui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mao-Juan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan-Qing","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Ting","family":"Qi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Lei","family":"Miao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2060","published-online":{"date-parts":[[2025,2,14]]},"reference":[{"key":"B1","unstructured":"Head KA, Jurenka JS. 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