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Exposure to sweat produced in a fear-inducing context triggers in its receivers a simulacrum of this emotional state, as evidenced by increased <jats:italic>medial frontalis<\/jats:italic> and <jats:italic>corrugator supercilii<\/jats:italic> (facial electromyography; fEMG) activity \u2013 two facial muscles involved in the display of fear facial expressions. However, despite the increased interest in the effects of emotional sweat, little is known about the properties of these chemical sweat samples. The goal of this study was to examine whether a second application of the same sweat sample would yield reliable results. Specifically, we assessed whether sweat samples collected from Portuguese males (<jats:italic>N =<\/jats:italic> 8) in fear (vs. neutral)-inducing contexts would produce similar fEMG activations (i.e., in the <jats:italic>medial frontalis<\/jats:italic> and <jats:italic>corrugator supercilii<\/jats:italic>) in female receivers (<jats:italic>N =<\/jats:italic> 60) across two independent applications (the first with Dutch and the second with Portuguese receivers). Our findings showed that exposure to fear (vs. neutral) sweat resulted in higher activation of both muscles compared with neutral odors, revealing a similar data pattern across the two applications and underlining the feasibility of reusing emotional sweat samples. The implications of these findings for properties of these sweat volatiles are discussed.<\/jats:p>","DOI":"10.3758\/s13428-020-01412-5","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T22:04:22Z","timestamp":1590098662000},"page":"2438-2451","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The lasting smell of emotions: The effects of reutilizing fear sweat samples"],"prefix":"10.3758","volume":"52","author":[{"given":"Nuno","family":"Gomes","sequence":"first","affiliation":[]},{"given":"F\u00e1bio","family":"Silva","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5304-5566","authenticated-orcid":false,"given":"G\u00fcn R.","family":"Semin","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,5,21]]},"reference":[{"issue":"5","key":"1412_CR1","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1093\/chemse\/bjj046","volume":"31","author":"D Chen","year":"2006","unstructured":"Chen, D., Katdare, A., & Lucas, N. 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