{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:00:19Z","timestamp":1792710019541,"version":"4.5.0"},"reference-count":21,"publisher":"MyJove Corporation","issue":"41","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/1980","type":"journal-article","created":{"date-parts":[[2010,8,5]],"date-time":"2010-08-05T15:29:19Z","timestamp":1281022159000},"source":"Crossref","is-referenced-by-count":25,"title":["<em>In vivo<\/em> Imaging of Transgenic <em>Leishmania<\/em> Parasites in a Live Host"],"prefix":"10.3791","author":[{"given":"Colin J.","family":"Thalhofer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel W.","family":"Graff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie","family":"Love-Homan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Hickerson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Craft","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen M.","family":"Beverley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2010,7,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"14006","DOI":"10.1074\/jbc.M610869200","type":"journal-article","article-title":"Two functionally divergent UDP-Gal nucleotide sugar transporters participate in phosphoglycan synthesis in Leishmania major","volume":"282","author":"Capul","year":"2007","journal-title":"J Biol Chem"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"9736","DOI":"10.1073\/pnas.87.24.9736","type":"journal-article","article-title":"Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes","volume":"87","author":"LeBowitz","year":"1990","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.molbiopara.2007.05.007","type":"journal-article","article-title":"Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers","volume":"155","author":"Mureev","year":"2007","journal-title":"Mol Biochem Parasitol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1128\/IAI.68.2.809-814.2000","type":"journal-article","article-title":"Priming of a beta-galactosidase (beta-GAL)-specific type 1 response in BALB\/c mice infected with beta-GAL-transfected Leishmania major","volume":"68","author":"Chakkalath","year":"2000","journal-title":"Infect Immun"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1126\/science.6701528","type":"journal-article","article-title":"Identification of an infective stage of Leishmania promastigotes","volume":"223","author":"Sacks","year":"1984","journal-title":"Science"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2802","DOI":"10.1128\/iai.55.11.2802-2806.1987","type":"journal-article","article-title":"Metacyclogenesis is a major determinant of Leishmania promastigote virulence and attenuation","volume":"55","author":"da Silva","year":"1987","journal-title":"Infect Immun"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1006\/expr.2001.4656","type":"journal-article","article-title":"A lipophosphoglycan-independent method for isolation of infective Leishmania metacyclic promastigotes by density gradient centrifugation","volume":"99","author":"Spath","year":"2001","journal-title":"Exp Parasitol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.4049\/jimmunol.144.3.1075","type":"journal-article","article-title":"Protection against Leishmania major in BALB\/c mice by adoptive transfer of a T cell clone recognizing a low molecular weight antigen released by promastigotes","volume":"144","author":"Scott","year":"1990","journal-title":"J Immunol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1084\/jem.194.10.1497","type":"journal-article","article-title":"The role of interleukin (IL)-10 in the persistence of Leishmania major in the skin after healing and the therapeutic potential of anti-IL-10 receptor antibody for sterile cure","volume":"194","author":"Belkaid","year":"2001","journal-title":"J Exp Med"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.4049\/jimmunol.156.6.2231","type":"journal-article","article-title":"Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi","volume":"156","author":"Wilson","year":"1996","journal-title":"J Immunol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1084\/jem.167.6.1927","type":"journal-article","article-title":"The dynamics of granuloma formation in experimental visceral leishmaniasis","volume":"167","author":"McElrath","year":"1988","journal-title":"J Exp Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"5486","DOI":"10.4049\/jimmunol.176.9.5486","type":"journal-article","article-title":"Loss of dendritic cell migration and impaired resistance to Leishmania donovani infection in mice deficient in CCL19 and CCL21","volume":"176","author":"Ato","year":"2006","journal-title":"J Immunol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1128\/IAI.71.1.401-410.2003","type":"journal-article","article-title":"Intradermal infection model for pathogenesis and vaccine studies of murine visceral leishmaniasis","volume":"71","author":"Ahmed","year":"2003","journal-title":"Infect Immun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.jim.2007.08.004","type":"journal-article","article-title":"Hock immunization: a humane alternative to mouse footpad injections","volume":"328","author":"Kamala","year":"2007","journal-title":"J Immunol Methods"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1462-5822.2004.00468.x","type":"journal-article","article-title":"Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice","volume":"7","author":"Lang","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0166-6851(00)00346-7","type":"journal-article","article-title":"Regulation of GP63 mRNA stability in promastigotes of virulent and attenuated Leishmania chagasi","volume":"112","author":"Brittingham","year":"2001","journal-title":"Mol Biochem Parasitol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0166-6851(00)00270-X","type":"journal-article","article-title":"Episomal and stable expression of the luciferase reporter gene for quantifying Leishmania spp. infections in macrophages and in animal models","volume":"110","author":"Roy","year":"2000","journal-title":"Mol Biochem Parasitol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1111\/j.1365-2958.1995.mmi_18040593.x","type":"journal-article","article-title":"Photonic detection of bacterial pathogens in living hosts","volume":"18","author":"Contag","year":"1995","journal-title":"Mol Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1128\/IAI.74.3.1819-1827.2006","type":"journal-article","article-title":"Induced Biliary Excretion of Listeria monocytogenes","volume":"74","author":"Hardy","year":"2006","journal-title":"Infect. Immun"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e34","DOI":"10.1371\/journal.pntd.0000034","type":"journal-article","article-title":"Optimization of Topical Therapy for Leishmania major Localized Cutaneous Leishmaniasis Using a Reliable C57BL\/6 Model","volume":"1","author":"Lecoeur","year":"2007","journal-title":"PLoS Negl Trop Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.pt.2009.07.006","type":"journal-article","article-title":"Imaging Leishmania development in their host cells","volume":"25","author":"Lang","year":"2009","journal-title":"Trends Parasitol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/1980\/in-vivo-imaging-of-transgenic-leishmania-parasites-in-a-live-host","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:42:50Z","timestamp":1792521770000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/1980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,27]]},"references-count":21,"journal-issue":{"issue":"41","published-online":{"date-parts":[[2010]]}},"URL":"https:\/\/doi.org\/10.3791\/1980","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,27]]}}}