{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:41:21Z","timestamp":1792701681009,"version":"4.5.0"},"reference-count":12,"publisher":"MyJove Corporation","issue":"60","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/3623","type":"journal-article","created":{"date-parts":[[2012,2,3]],"date-time":"2012-02-03T22:20:54Z","timestamp":1328307654000},"source":"Crossref","is-referenced-by-count":4,"title":["High-throughput Detection Method for Influenza Virus"],"prefix":"10.3791","author":[{"given":"Pawan","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison E.","family":"Bartoszek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Gorski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjib","family":"Bhattacharyya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose F.","family":"Navidad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica S.","family":"Thakar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subramaniam","family":"Malarkannan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2012,2,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1126\/science.94.2427.22","type":"journal-article","article-title":"The agglutination of red cells by allantoic fluid of chick embryos infected with influenza virus","volume":"94","author":"Hirst","year":"1941","unstructured":"Hirst, G.K. The agglutination of red cells by allantoic fluid of chick embryos infected with influenza virus. Science. 94, 22-23 (1941).","journal-title":"Science"},{"key":"ref2","first-page":"149","type":"journal-article","article-title":"Real-time polymerase chain reaction as a rapid and efficient alternative to estimation of picornavirus titers by tissue culture infectious dose 50% or plaque forming units","volume":"53","author":"Jonsson","year":"2009","unstructured":"Jonsson, N., Gullberg, M., & Lindberg, A.M. Real-time polymerase chain reaction as a rapid and efficient alternative to estimation of picornavirus titers by tissue culture infectious dose 50% or plaque forming units. Microbiol. Immunol. 53, 149-154 (2009).","journal-title":"Microbiol. Immunol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1126\/science.110.2851.184","type":"journal-article","article-title":"Hemagglutinating Behavior of Mouse and Egg-adapted Type A (PR8) Influenza Virus","volume":"110","author":"Kalter","year":"1949","unstructured":"Kalter, S.S. Hemagglutinating Behavior of Mouse and Egg-adapted Type A (PR8) Influenza Virus. Science. 110, 184 (1949).","journal-title":"Science"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1073\/pnas.38.8.747","type":"journal-article","article-title":"Production of Plaques in Monolayer Tissue Cultures by Single Particles of an Animal Virus","volume":"38","author":"Dulbecco","year":"1952","unstructured":"Dulbecco, R. Production of Plaques in Monolayer Tissue Cultures by Single Particles of an Animal Virus. Proc. Natl. Acad. Sci. U. S. A. 38, 747-752 (1952).","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1128\/AAC.01446-08","type":"journal-article","article-title":"Detection of molecular markers of antiviral resistance in influenza A (H5N1) viruses using a pyrosequencing method","volume":"53","author":"Deyde","year":"2009","unstructured":"Deyde, V.M., Nguyen, T., Bright, R.A., Balish, A., Shu, B., Lindstrom, S., Klimov, A.I., & Gubareva, L.V. Detection of molecular markers of antiviral resistance in influenza A (H5N1) viruses using a pyrosequencing method. Antimicrob. Agents Chemother. 53, 1039-1047 (2009).","journal-title":"Antimicrob. Agents Chemother"},{"key":"ref6","first-page":"149","type":"journal-article","article-title":"Real-time polymerase chain reaction as a rapid and efficient alternative to estimation of picornavirus titers by tissue culture infectious dose 50% or plaque forming units","volume":"53","author":"Jonsson","year":"2009","unstructured":"Jonsson, N., Gullberg, M., & Lindberg, A.M. Real-time polymerase chain reaction as a rapid and efficient alternative to estimation of picornavirus titers by tissue culture infectious dose 50% or plaque forming units. Microbiol. Immunol. 53, 149-154 (2009).","journal-title":"Microbiol. Immunol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.diagmicrobio.2007.03.011","type":"journal-article","article-title":"Performance of Directigen flu A+B enzyme immunoassay and direct fluorescent assay for detection of influenza infection during the 2004-2005 season","volume":"58","author":"Rahman","year":"2007","unstructured":"Rahman, M., Kieke, B.A., Vandermause, M.F., Mitchell, P.D., Greenlee, R.T., & Belongia, E.A. Performance of Directigen flu A+B enzyme immunoassay and direct fluorescent assay for detection of influenza infection during the 2004-2005 season. Diagnostic Microbiology and Infectious Disease. 58, 413-418 (2007).","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3407","DOI":"10.1128\/JCM.41.7.3407-3409.2003","type":"journal-article","article-title":"Suboptimal detection of influenza virus in adults by the Directigen Flu A+B enzyme immunoassay and correlation of results with the number of antigen-positive cells detected by cytospin immunofluorescence","volume":"41","author":"Landry","year":"2003","unstructured":"Landry, M.L. & Ferguson, D. Suboptimal detection of influenza virus in adults by the Directigen Flu A+B enzyme immunoassay and correlation of results with the number of antigen-positive cells detected by cytospin immunofluorescence. J. Clin. Microbiol. 41, 3407-3409 (2003).","journal-title":"J. Clin. Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1128\/JCM.39.1.134-138.2001","type":"journal-article","article-title":"Simultaneous detection and typing of influenza viruses A and B by a nested reverse transcription-PCR: comparison to virus isolation and antigen detection by immunofluorescence and optical immunoassay (FLU OIA)","volume":"39","author":"Herrmann","year":"2001","unstructured":"Herrmann, B., Larsson, C., & Zweygberg, B.W. Simultaneous detection and typing of influenza viruses A and B by a nested reverse transcription-PCR: comparison to virus isolation and antigen detection by immunofluorescence and optical immunoassay (FLU OIA). J. Clin. Microbiol. 39, 134-138 (2001).","journal-title":"J. Clin. Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","type":"journal-article","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","unstructured":"Reed, L.J. & Muench H. A simple method of estimating fifty percent endpoints. Am. J. Epidemiol. 27, 493-497 (1938).","journal-title":"Am. J. Epidemiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/nmeth0709-482a","type":"journal-article","article-title":"Fluorescent proteins: into the infrared","volume":"6","author":"Doerr","year":"2009","unstructured":"Doerr, A. Fluorescent proteins: into the infrared. Nat. Meth. 6, 482-483 (2009).","journal-title":"Nat. Meth"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1128\/JCM.02636-10","type":"journal-article","article-title":"Design and performance of the CDC real-time reverse transcriptase PCR swine flu panel for detection of 2009 A (H1N1) pandemic influenza virus","volume":"49","author":"Shu","year":"2011","unstructured":"Shu, B., Wu, KH., Emery, S., Villanueva, J., Johnson, R., Guthrie, E., Berman, L., Warnes, C., Barnes, N., Klimov, A., & Lindstrom, S. Design and performance of the CDC real-time reverse transcriptase PCR swine flu panel for detection of 2009 A (H1N1) pandemic influenza virus. J. Clin. Microbiol. 49, 2614-2619 (2011).","journal-title":"J. Clin. Microbiol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/3623\/high-throughput-detection-method-for-influenza-virus","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:10:31Z","timestamp":1792699831000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/3623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,4]]},"references-count":12,"journal-issue":{"issue":"60","published-online":{"date-parts":[[2012]]}},"URL":"https:\/\/doi.org\/10.3791\/3623","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,4]]}}}