{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:49:38Z","timestamp":1792738178498,"version":"4.5.0"},"reference-count":37,"publisher":"MyJove Corporation","issue":"81","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/50905","type":"journal-article","created":{"date-parts":[[2013,12,4]],"date-time":"2013-12-04T20:24:20Z","timestamp":1386188660000},"source":"Crossref","is-referenced-by-count":12,"title":["<em>In vivo<\/em> Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices"],"prefix":"10.3791","author":[{"given":"Martina","family":"Sonego","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine Julie","family":"Oudin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Doherty","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Lalli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2013,11,25]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.neuron.2011.05.001","type":"journal-article","article-title":"Adult neurogenesis in the mammalian brain: significant answers and significant questions","volume":"70","author":"Ming","year":"2011","unstructured":"Ming, G. L. & Song, H. Adult neurogenesis in the mammalian brain: significant answers and significant questions. Neuron. 70, 687-702, doi:10.1016\/j.neuron.2011.05.001 (2011).","journal-title":"Neuron"},{"key":"ref2","first-page":"339","type":"journal-article","article-title":"New neurons and new memories: how does adult hippocampal neurogenesis affect learning and","volume":"11","author":"Deng","year":"2010","unstructured":"Deng, W., Aimone, J. B. & Gage, F. H. New neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory? Nat. Rev. Neurosci. 11, 339-350, doi:10.1038\/nrn2822 (2010)."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.tins.2010.09.006","type":"journal-article","article-title":"Is adult neurogenesis essential for olfaction?","volume":"34","author":"Lazarini","year":"2011","unstructured":"Lazarini, F. & Lledo, P. M. Is adult neurogenesis essential for olfaction? Trends Neurosci. 34, 20-30, doi:10.1016\/j.tins.2010.09.006 (2011).","journal-title":"Trends Neurosci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1038\/nm747","type":"journal-article","article-title":"Neuronal replacement from endogenous precursors in the adult brain after stroke","volume":"8","author":"Arvidsson","year":"2002","unstructured":"Arvidsson, A., Collin, T., Kirik, D., Kokaia, Z. & Lindvall, O. Neuronal replacement from endogenous precursors in the adult brain after stroke. Nat. Med. 8, 963-970, doi:10.1038\/nm747 (2002).","journal-title":"Nat. Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0014-4886(03)00209-7","type":"journal-article","article-title":"alpha6beta1 integrin directs migration of neuronal precursors in adult mouse forebrain","volume":"183","author":"Emsley","year":"2003","unstructured":"Emsley, J. G. & Hagg, T. alpha6beta1 integrin directs migration of neuronal precursors in adult mouse forebrain. Exp. Neurol. 183, 273-285 (2003).","journal-title":"Exp. Neurol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1097\/01.jnen.0000190066.13312.8f","type":"journal-article","article-title":"Subventricular zone neuroblasts emigrate toward cortical lesions","volume":"64","author":"Sundholm-Peters","year":"2005","unstructured":"Sundholm-Peters, N. L., Yang, H. K., Goings, G. E., Walker, A. S. & Szele, F. G. Subventricular zone neuroblasts emigrate toward cortical lesions. J. Neuropathol. Exp. Neurol. 64, 1089-1100 (2005).","journal-title":"J. Neuropathol. Exp. Neurol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"14895","DOI":"10.1073\/pnas.93.25.14895","type":"journal-article","article-title":"Network of tangential pathways for neuronal migration in adult mammalian brain","volume":"93","author":"Doetsch","year":"1996","unstructured":"Doetsch, F. & Alvarez-Buylla, A. Network of tangential pathways for neuronal migration in adult mammalian brain. Proc. Natl. Acad. Sci. U.S.A. 93, 14895-14900 (1996).","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1146\/annurev.neuro.28.051804.101459","type":"journal-article","article-title":"Adult neurogenesis in the mammalian central nervous system","volume":"28","author":"Ming","year":"2005","unstructured":"Ming, G. L. & Song, H. Adult neurogenesis in the mammalian central nervous system. Annu. Rev. Neurosci. 28, 223-250, doi:10.1146\/annurev.neuro.28.051804.101459 (2005).","journal-title":"Annu. Rev. Neurosci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.neuropharm.2010.01.010","type":"journal-article","article-title":"A symphony of signals conducts early and late stages of adult neurogenesis","volume":"58","author":"Pathania","year":"2010","unstructured":"Pathania, M., Yan, L. D. & Bordey, A. A symphony of signals conducts early and late stages of adult neurogenesis. Neuropharmacology. 58, 865-876, doi:10.1016\/j.neuropharm.2010.01.010 (2010).","journal-title":"Neuropharmacology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"7551","DOI":"10.1523\/JNEUROSCI.6716-10.2011","type":"journal-article","article-title":"Abnormal neuronal migration changes the fate of developing neurons in the postnatal olfactory bulb","volume":"31","author":"Belvindrah","year":"2011","unstructured":"Belvindrah, R., Nissant, A. & Lledo, P. M. Abnormal neuronal migration changes the fate of developing neurons in the postnatal olfactory bulb. J. Neurosci. 31, 7551-7562, doi:10.1523\/JNEUROSCI.6716-10.2011 (2011).","journal-title":"J. Neurosci"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1002\/cne.20609","type":"journal-article","article-title":"Mechanisms of subventricular zone expansion after focal cortical ischemic injury","volume":"488","author":"Gotts","year":"2005","unstructured":"Gotts, J. E. & Chesselet, M. F. Mechanisms of subventricular zone expansion after focal cortical ischemic injury. J. Comp. Neurol. 488, 201-214, doi:10.1002\/cne.20609 (2005).","journal-title":"J. Comp. Neurol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.brainres.2003.10.034","type":"journal-article","article-title":"Migration patterns of subventricular zone cells in adult mice change after cerebral cortex injury","volume":"996","author":"Goings","year":"2004","unstructured":"Goings, G. E., Sahni, V. & Szele, F. G. Migration patterns of subventricular zone cells in adult mice change after cerebral cortex injury. Brain Res. 996, 213-226 (2004).","journal-title":"Brain Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.pneurobio.2004.07.001","type":"journal-article","article-title":"Roles of the mammalian subventricular zone in cell replacement after brain injury","volume":"74","author":"Romanko","year":"2004","unstructured":"Romanko, M. J. et al. Roles of the mammalian subventricular zone in cell replacement after brain injury. Prog. Neurobiol. 74, 77-99, doi:10.1016\/j.pneurobio.2004.07.001 (2004).","journal-title":"Prog. Neurobiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1038\/nprot.2006.276","type":"journal-article","article-title":"In vivo electroporation in the embryonic mouse central nervous system","volume":"1","author":"Saito","year":"2006","unstructured":"Saito, T. In vivo electroporation in the embryonic mouse central nervous system. Nat. Protoc. 1, 1552-1558, doi:10.1038\/nprot.2006.276 (2006).","journal-title":"Nat. Protoc"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e1883","DOI":"10.1371\/journal.pone.0001883","type":"journal-article","article-title":"Efficient in vivo electroporation of the postnatal rodent forebrain","volume":"3","author":"Boutin","year":"2008","unstructured":"Boutin, C., Diestel, S., Desoeuvre, A., Tiveron, M. C. & Cremer, H. Efficient in vivo electroporation of the postnatal rodent forebrain. PloS One. 3, e1883, doi:10.1371\/journal.pone.0001883 (2008).","journal-title":"PloS One"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1038\/nmeth.1174","type":"journal-article","article-title":"Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation","volume":"5","author":"Barnabe-Heider","year":"2008","unstructured":"Barnabe-Heider, F. et al. Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nat. Methods 5, 189-196, doi:10.1038\/nmeth.1174 (2008).","journal-title":"Nat. Methods"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/j.neuron.2010.03.009","type":"journal-article","article-title":"NMDA receptors activated by subventricular zone astrocytic glutamate are critical for neuroblast survival prior to entering a synaptic network","volume":"65","author":"Platel","year":"2010","unstructured":"Platel, J. C. et al. NMDA receptors activated by subventricular zone astrocytic glutamate are critical for neuroblast survival prior to entering a synaptic network. Neuron. 65, 859-872, doi:10.1016\/j.neuron.2010.03.009 (2010).","journal-title":"Neuron"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e38174","DOI":"10.1371\/journal.pone.0038174","type":"journal-article","article-title":"miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons","volume":"7","author":"Pathania","year":"2012","unstructured":"Pathania, M. et al. miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. PloS One. 7, e38174, doi:10.1371\/journal.pone.0038174 (2012).","journal-title":"PloS One"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1038\/ncomms1961","type":"journal-article","article-title":"High-performance and site-directed in utero electroporation by a triple-electrode probe","volume":"3","author":"dal Maschio","year":"2012","unstructured":"dal Maschio, M. et al. High-performance and site-directed in utero electroporation by a triple-electrode probe. Nat. Commun. 3, 960, doi:10.1038\/ncomms1961 (2012).","journal-title":"Nat. Commun"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1523\/JNEUROSCI.5483-10.2011","type":"journal-article","article-title":"Endocannabinoids regulate the migration of subventricular zone-derived neuroblasts in the postnatal brain","volume":"31","author":"Oudin","year":"2011","unstructured":"Oudin, M. J. et al. Endocannabinoids regulate the migration of subventricular zone-derived neuroblasts in the postnatal brain. J. Neurosci. 31, 4000-4011, doi:10.1523\/JNEUROSCI.5483-10.2011 (2011).","journal-title":"J. Neurosci"},{"key":"ref21","type":"journal-article","article-title":"Imaging and recording subventricular zone progenitor cells in live tissue of postnatal mice","volume":"4","author":"Lacar","year":"2010","unstructured":"Lacar, B., Young, S. Z., Platel, J. C. & Bordey, A. Imaging and recording subventricular zone progenitor cells in live tissue of postnatal mice. Front. Neurosci. 4, doi:10.3389\/fnins.2010.00043 (2010).","journal-title":"Front. Neurosci"},{"issue":"72","key":"ref22","first-page":"e50197","type":"journal-article","article-title":"Neonatal subventricular zone electroporation","author":"Feliciano","year":"2013","unstructured":"Feliciano, D. M., Lafourcade, C. A. & Bordey, A. Neonatal subventricular zone electroporation. J. Vis. Exp. 72, e50197, doi:10.3791\/50197 (2013).","journal-title":"J. Vis. Exp"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1002\/cne.21473","type":"journal-article","article-title":"Dynamic features of postnatal subventricular zone cell motility: a two-photon time-lapse study","volume":"505","author":"Nam","year":"2007","unstructured":"Nam, S. C. et al. Dynamic features of postnatal subventricular zone cell motility: a two-photon time-lapse study. J. Comp. Neurol. 505, 190-208, doi:10.1002\/cne.21473 (2007).","journal-title":"J. Comp. Neurol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3389\/fnins.2011.00030","type":"journal-article","article-title":"Subventricular zone cell migration: lessons from quantitative two-photon microscopy","volume":"5","author":"James","year":"2011","unstructured":"James, R., Kim, Y., Hockberger, P. E. & Szele, F. G. Subventricular zone cell migration: lessons from quantitative two-photon microscopy. Front. Neurosci. 5, 30, doi:10.3389\/fnins.2011.00030 (2011).","journal-title":"Front. Neurosci"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1749-8104-6-13","type":"journal-article","article-title":"Targeted electroporation of defined lateral ventricular walls: a novel and rapid method to study fate specification during postnatal forebrain neurogenesis","volume":"6","author":"Fernandez","year":"2011","unstructured":"Fernandez, M. E., Croce, S., Boutin, C., Cremer, H. & Raineteau, O. Targeted electroporation of defined lateral ventricular walls: a novel and rapid method to study fate specification during postnatal forebrain neurogenesis. Neural Dev. 6, 13, doi:10.1186\/1749-8104-6-13 (2011).","journal-title":"Neural Dev"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"16892","DOI":"10.1523\/JNEUROSCI.0344-12.2012","type":"journal-article","article-title":"Plexin-b2 regulates the proliferation and migration of neuroblasts in the postnatal and adult subventricular zone","volume":"32","author":"Saha","year":"2012","unstructured":"Saha, B., Ypsilanti, A. R., Boutin, C., Cremer, H. & Chedotal, A. Plexin-b2 regulates the proliferation and migration of neuroblasts in the postnatal and adult subventricular zone. J. Neurosci. 32, 16892-16905, doi:10.1523\/JNEUROSCI.0344-12.2012 (2012).","journal-title":"J. Neurosci"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/nature10487","type":"journal-article","article-title":"Corridors of migrating neurons in the human brain and their decline during infancy","volume":"478","author":"Sanai","year":"2011","unstructured":"Sanai, N. et al. Corridors of migrating neurons in the human brain and their decline during infancy. Nature. 478, 382-386, doi:10.1038\/nature10487 (2011).","journal-title":"Nature"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.neuroscience.2004.04.061","type":"journal-article","article-title":"Neurogenesis in the striatum of the quinolinic acid lesion model of Huntington's disease","volume":"127","author":"Tattersfield","year":"2004","unstructured":"Tattersfield, A. S. et al. Neurogenesis in the striatum of the quinolinic acid lesion model of Huntington's disease. Neuroscience. 127, 319-332, doi:10.1016\/j.neuroscience.2004.04.061 (2004).","journal-title":"Neuroscience"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0378-1119(91)90434-D","type":"journal-article","article-title":"Efficient selection for high-expression transfectants with a novel eukaryotic vector","volume":"108","author":"Niwa","year":"1991","unstructured":"Niwa, H., Yamamura, K. & Miyazaki, J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 108, 193-199 (1991).","journal-title":"Gene"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1038\/nrn1867","type":"journal-article","article-title":"Adult neurogenesis and functional plasticity in neuronal circuits","volume":"7","author":"Lledo","year":"2006","unstructured":"Lledo, P. M., Alonso, M. & Grubb, M. S. Adult neurogenesis and functional plasticity in neuronal circuits. Nat. Rev. Neurosci. 7, 179-193, doi:10.1038\/nrn1867 (2006).","journal-title":"Nat. Rev. Neurosci"},{"issue":"67","key":"ref31","first-page":"e4061","type":"journal-article","article-title":"Time-lapse imaging of neuroblast migration in acute slices of the adult mouse forebrain","author":"Khlghatyan","year":"2012","unstructured":"Khlghatyan, J. & Saghatelyan, A. Time-lapse imaging of neuroblast migration in acute slices of the adult mouse forebrain. J. Vis. Exp. 67, e4061, doi:10.3791\/4061 (2012).","journal-title":"J. Vis. Exp"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1523\/JNEUROSCI.4956-08.2009","type":"journal-article","article-title":"Vasculature guides migrating neuronal precursors in the adult mammalian forebrain via brain-derived neurotrophic factor signaling","volume":"29","author":"Snapyan","year":"2009","unstructured":"Snapyan, M. et al. Vasculature guides migrating neuronal precursors in the adult mammalian forebrain via brain-derived neurotrophic factor signaling. J. Neurosci. 29, 4172-4188, doi:10.1523\/JNEUROSCI.4956-08.2009 (2009).","journal-title":"J. Neurosci"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"3783","DOI":"10.1113\/jphysiol.2008.155879","type":"journal-article","article-title":"Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole-mounts of the subventricular zone","volume":"586","author":"Platel","year":"2008","unstructured":"Platel, J. C., Heintz, T., Young, S., Gordon, V. & Bordey, A. Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole-mounts of the subventricular zone. J. Physiol. 586, 3783-3793, doi:10.1113\/jphysiol.2008.155879 (2008).","journal-title":"J. Physiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"13652","DOI":"10.1073\/pnas.0506008102","type":"journal-article","article-title":"Cytoskeletal coordination during neuronal migration","volume":"102","author":"Schaar","year":"2005","unstructured":"Schaar, B. T. & McConnell, S. K. Cytoskeletal coordination during neuronal migration. Proc. Natl. Acad. Sci. U.S.A. 102, 13652-13657, doi:10.1073\/pnas.0506008102 (2005).","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.conb.2009.12.003","type":"journal-article","article-title":"Neuronal migration mechanisms in development and disease","volume":"20","author":"Valiente","year":"2010","unstructured":"Valiente, M. & Marin, O. Neuronal migration mechanisms in development and disease. Curr.Opin. Neurobiol. 20, 68-78, doi:10.1016\/j.conb.2009.12.003 (2010).","journal-title":"Curr.Opin. Neurobiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.1242\/jcs.079954","type":"journal-article","article-title":"Galectin-3 maintains cell motility from the subventricular zone to the olfactory bulb","volume":"124","author":"Comte","year":"2011","unstructured":"Comte, I. et al. Galectin-3 maintains cell motility from the subventricular zone to the olfactory bulb. J. Cell Sci. 124, 2438-2447, doi:10.1242\/jcs.079954 (2011).","journal-title":"J. Cell Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"12171","DOI":"10.1523\/JNEUROSCI.0653-13.2013","type":"journal-article","article-title":"Fascin regulates the migration of subventricular zone-derived neuroblasts in the postnatal brain","volume":"33","author":"Sonego","year":"2013","unstructured":"Sonego M., et al. Fascin regulates the migration of subventricular zone-derived neuroblasts in the postnatal brain. J. Neurosci. 33, 12171-12185, doi:10.1523\/JNEUROSCI.0653-13.2013 (2013).","journal-title":"J. Neurosci"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/50905\/in-vivo-postnatal-electroporation-time-lapse-imaging-neuroblast","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:35:29Z","timestamp":1792694129000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/50905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,25]]},"references-count":37,"journal-issue":{"issue":"81","published-online":{"date-parts":[[2013]]}},"URL":"https:\/\/doi.org\/10.3791\/50905","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,25]]}}}