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Journal of Bacteriology. 179, 1533-1540 (1997).","journal-title":"Journal of Bacteriology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1038\/ncb1964","type":"journal-article","article-title":"The bacterial virulence factor InlC perturbs apical cell junctions and promotes cell-to-cell spread of Listeria","volume":"11","author":"Rajabian","year":"2009","unstructured":"Rajabian, T., et al. The bacterial virulence factor InlC perturbs apical cell junctions and promotes cell-to-cell spread of Listeria. Nature Cell Biology. 11, 1212-1218, doi: 10.1038\/ncb1964 (2009).","journal-title":"Nature Cell Biology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"17333","DOI":"10.1073\/pnas.1007765107","type":"journal-article","article-title":"The Listeria monocytogenes InlC protein interferes with innate immune responses by targeting the I\u03baB kinase subinit IKK\u03b1","volume":"107","author":"Gouin","year":"2010","unstructured":"Gouin, E., et al. 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Methods in Molecular Microbiology. 31, 161-177 (2002).","journal-title":"Methods in Molecular Microbiology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/nature08282","type":"journal-article","article-title":"Population context determines cell-to-cell variability in endocytosis and virus infection","volume":"461","author":"Snijder","year":"2009","unstructured":"Snijder, B., Sacher, R., R\u00e4m\u00f6, P., Damm, E.M., Liberali, P., & Pelkmans, L. Population context determines cell-to-cell variability in endocytosis and virus infection. 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