{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:41:42Z","timestamp":1792690902552,"version":"4.5.0"},"reference-count":28,"publisher":"MyJove Corporation","issue":"92","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/51612","type":"journal-article","created":{"date-parts":[[2014,10,16]],"date-time":"2014-10-16T19:59:50Z","timestamp":1413489590000},"source":"Crossref","is-referenced-by-count":7,"title":["Combined <em>In vivo<\/em> Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice"],"prefix":"10.3791","author":[{"given":"Nicholas M.","family":"Bernthal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brad N.","family":"Taylor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A.","family":"Meganck","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan H.","family":"Shahbazian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared A.","family":"Niska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P.","family":"Francis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lloyd S.","family":"Miller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2014,10,16]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.copbio.2009.01.007","type":"journal-article","article-title":"Advances in bioluminescence imaging of live animal models","volume":"20","author":"Dothager","year":"2009","unstructured":"Dothager, R. S. et al. Advances in bioluminescence imaging of live animal models. Curr. Opin. Biotechnol. 20, 45-53, DOI: S0958-1669(09)00006-8 [pii];10.1016\/j.copbio.2009.01.007 [doi] (2009).","journal-title":"Curr Opin Biotechnol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/j.tibtech.2011.06.010","type":"journal-article","article-title":"Bioluminescence imaging progress and applications","volume":"29","author":"Badr","year":"2011","unstructured":"Badr, C. E., & Tannous, B. A. Bioluminescence imaging: progress and applications. Trends Biotechnol. 29, 624-633, DOI: S0167-7799(11)00114-4 [pii];10.1016\/j.tibtech.2011.06.010 [doi] (2011).","journal-title":"Trends Biotechnol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2967\/jnumed.107.045799","type":"journal-article","article-title":"Optical imaging current applications and future directions","volume":"49","author":"Luker","year":"2008","unstructured":"Luker, G. D., & Luker, K. E. Optical imaging: current applications and future directions. J. Nucl. Med. 49, 1-4, DOI: jnumed.107.045799 [pii];10.2967\/jnumed.107.045799 [doi] (2008).","journal-title":"J Nucl Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/nbt1074","type":"journal-article","article-title":"Looking and listening to light the evolution of whole-body photonic imaging","volume":"23","author":"Ntziachristos","year":"2005","unstructured":"Ntziachristos, V., Ripoll, J., Wang, L. V., & Weissleder, R. Looking and listening to light: the evolution of whole-body photonic imaging. Nat. Biotechnol. 23, 313-320, DOI: nbt1074 [pii];10.1038\/nbt1074 [doi] (2005).","journal-title":"Nat Biotechnol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.tibtech.2009.11.004","type":"journal-article","article-title":"Musculoskeletal molecular imaging a comprehensive overview","volume":"28","author":"Reumann","year":"2010","unstructured":"Reumann, M. K., Weiser, M. C., & Mayer-Kuckuk, P. Musculoskeletal molecular imaging: a comprehensive overview. Trends Biotechnol. 28, 93-101, DOI: S0167-7799(09)00217-0 [pii];10.1016\/j.tibtech.2009.11.004 [doi] (2010).","journal-title":"Trends Biotechnol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.bone.2010.07.027","type":"journal-article","article-title":"Optical advances in skeletal imaging applied to bone metastases","volume":"48","author":"Snoeks","year":"2011","unstructured":"Snoeks, T. J., Khmelinskii, A., Lelieveldt, B. P., Kaijzel, E. L., & Lowik, C. W. Optical advances in skeletal imaging applied to bone metastases. Bone. 48, 106-114, DOI: S8756-3282(10)01396-7 [pii];10.1016\/j.bone.2010.07.027 [doi] (2011).","journal-title":"Bone"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1016\/j.biomaterials.2009.11.068","type":"journal-article","article-title":"The potential for bio-optical imaging of biomaterial-associated infection in vivo","volume":"31","author":"Sjollema","year":"2010","unstructured":"Sjollema, J. et al. The potential for bio-optical imaging of biomaterial-associated infection in vivo., Biomaterials. 31, 1984-1995, DOI: S0142-9612(09)01312-X [pii];10.1016\/j.biomaterials.2009.11.068 [doi] (2010).","journal-title":"Biomaterials"},{"key":"ref8","type":"book","article-title":"Clinical practice. Infection associated with prosthetic joints","volume-title":"N Engl J Med","author":"Del Pozo","year":"2009","unstructured":"Del Pozo, J. L., & Patel, R. Clinical practice. Infection associated with prosthetic joints. N. Engl. J. Med. 361, 787-794, DOI: 361\/8\/787 [pii];10.1056\/NEJMcp0905029 [doi] (2009)."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e104","DOI":"10.2106\/JBJS.K.01417","type":"journal-article","article-title":"Management of periprosthetic joint infection the current knowledge AAOS exhibit selection","volume":"94","author":"Parvizi","year":"2012","unstructured":"Parvizi, J., Adeli, B., Zmistowski, B., Restrepo, C., & Greenwald, A. S. Management of periprosthetic joint infection: the current knowledge: AAOS exhibit selection. J. Bone Joint Surg. Am. 94, e104, DOI: 1216215 [pii];10.2106\/JBJS.K.01417 [doi] (2012).","journal-title":"J Bone Joint Surg Am"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"5967","DOI":"10.1016\/j.biomaterials.2012.05.031","type":"journal-article","article-title":"Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials","volume":"33","author":"Arciola","year":"2012","unstructured":"Arciola, C. R., Campoccia, D., Speziale, P., Montanaro, L., & Costerton, J. W. Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials. Biomaterials. 33, 5967-5982, DOI: S0142-9612(12)00572-8 [pii];10.1016\/j.biomaterials.2012.05.031 [doi] (2012).","journal-title":"Biomaterials"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/j.1574-695X.2012.00938.x","type":"journal-article","article-title":"Pathogenesis and treatment concepts of orthopaedic biofilm infections","volume":"65","author":"Zimmerli","year":"2012","unstructured":"Zimmerli, W., & Moser, C. Pathogenesis and treatment concepts of orthopaedic biofilm infections. FEMS Immunol. Med. Microbiol. 65, 158-168, DOI: 10.1111\/j.1574-695X.2012.00938.x [doi] (2012).","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1001\/2012.jama.11153","type":"journal-article","article-title":"Total knee arthroplasty volume utilization and outcomes among Medicare beneficiaries 1991-2010","volume":"308","author":"Cram","year":"2012","unstructured":"Cram, P. et al. Total knee arthroplasty volume, utilization, and outcomes among Medicare beneficiaries, 1991-2010, JAMA. 308, 1227-1236, DOI: 1362022 [pii];10.1001\/2012.jama.11153 [doi] (2012).","journal-title":"JAMA"},{"key":"ref13","doi-asserted-by":"crossref","DOI":"10.2106\/JBJS.K.00011","type":"journal-article","article-title":"Adverse outcomes in hip arthroplasty long-term trends","volume":"94","author":"Wolf","year":"2012","unstructured":"Wolf, B. R., Lu, X., Li, Y., Callaghan, J. J., & Cram, P. Adverse outcomes in hip arthroplasty: long-term trends. J. Bone Joint Surg. Am. 94, e103, DOI: 1216214 [pii];10.2106\/JBJS.K.00011 [doi] (2012).","journal-title":"J Bone Joint Surg Am"},{"key":"ref14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0012580","type":"journal-article","article-title":"A mouse model of post-arthroplasty Staphylococcus aureus joint infection to evaluate in vivo the efficacy of antimicrobial implant coatings","volume":"5","author":"Bernthal","year":"2010","unstructured":"Bernthal, N. M. et al. A mouse model of post-arthroplasty Staphylococcus aureus joint infection to evaluate in vivo the efficacy of antimicrobial implant coatings. PLoS. ONE. 5, e12580, DOI: 10.1371\/journal.pone.0012580 [doi] (2010).","journal-title":"PLoS ONE"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1002\/jor.21414","type":"journal-article","article-title":"Protective role of IL-1beta against post-arthroplasty Staphylococcus aureus infection","volume":"29","author":"Bernthal","year":"2011","unstructured":"Bernthal, N. M. et al. Protective role of IL-1beta against post-arthroplasty Staphylococcus aureus infection. J. Orthop. Res. 29, 1621-1626, DOI: 10.1002\/jor.21414 [doi] (2011).","journal-title":"J Orthop Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e47397","DOI":"10.1371\/journal.pone.0047397","type":"journal-article","article-title":"Monitoring bacterial burden, inflammation and bone damage longitudinally using optical and \u00b5CT imaging in an orthopaedic implant infection in mice","volume":"7","author":"Niska","year":"2012","unstructured":"Niska, J. A. et al. Monitoring bacterial burden, inflammation and bone damage longitudinally using optical and \u00b5CT imaging in an orthopaedic implant infection in mice. PLoS. ONE. 7, e47397, DOI: 10.1371\/journal.pone.0047397 [doi];PONE-D-12-17997 [pii] (2012).","journal-title":"PLoS ONE"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2590","DOI":"10.1128\/AAC.06291-11","type":"journal-article","article-title":"Daptomycin and tigecycline have broader effective dose ranges than vancomycin as prophylaxis against a Staphylococcus aureus surgical implant infection in mice","volume":"56","author":"Niska","year":"2012","unstructured":"Niska, J. A. et al. Daptomycin and tigecycline have broader effective dose ranges than vancomycin as prophylaxis against a Staphylococcus aureus surgical implant infection in mice. Antimicrob. Agents Chemother. 56, 2590-2597, DOI: AAC.06291-11 [pii];10.1128\/AAC.06291-11 [doi] (2012).","journal-title":"Antimicrob Agents Chemother"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"5080","DOI":"10.1128\/AAC.00702-13","type":"journal-article","article-title":"Vancomycin-Rifampin Combination Therapy has Enhanced Efficacy Against an Experimental Staphylococcus aureus Prosthetic Joint Infection","volume":"57","author":"Niska","year":"2013","unstructured":"Niska, J. A. et al. Vancomycin-Rifampin Combination Therapy has Enhanced Efficacy Against an Experimental Staphylococcus aureus Prosthetic Joint Infection. Antimicrob. Agents Chemother. 57, 5080-5086, DOI: AAC.00702-13 [pii];10.1128\/AAC.00702-13 [doi] (2013).","journal-title":"Antimicrob Agents Chemother"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/jor.21519","type":"journal-article","article-title":"Mouse model of chronic post-arthroplasty infection noninvasive in vivo bioluminescence imaging to monitor bacterial burden for long-term study","volume":"30","author":"Pribaz","year":"2012","unstructured":"Pribaz, J. R. et al. Mouse model of chronic post-arthroplasty infection: noninvasive in vivo bioluminescence imaging to monitor bacterial burden for long-term study. J. Orthop. Res. 30, 335-340, DOI: 10.1002\/jor.21519 [doi] (2012).","journal-title":"J Orthop Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1182\/blood.V96.2.719.014k29_719_726","type":"journal-article","article-title":"Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages","volume":"96","author":"Faust","year":"2000","unstructured":"Faust, N., Varas, F., Kelly, L. M., Heck, S., & Graf, T. Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages. Blood. 96, 719-726 (2000).","journal-title":"Blood"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1128\/IAI.71.2.882-890.2003","type":"journal-article","article-title":"Direct continuous method for monitoring biofilm infection in a mouse model","volume":"71","author":"Kadurugamuwa","year":"2003","unstructured":"Kadurugamuwa, J. L. et al. Direct continuous method for monitoring biofilm infection in a mouse model. Infect. Immun. 71, 882-890 (2003).","journal-title":"Infect Immun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"024007","DOI":"10.1117\/1.2717898","type":"journal-article","article-title":"Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging","volume":"12","author":"Kuo","year":"2007","unstructured":"Kuo, C., Coquoz, O., Troy, T. L., Xu, H., & Rice, B. W. Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging. J. Biomed. Opt. 12, 024007, DOI: 10.1117\/1.2717898 [doi] (2007).","journal-title":"J Biomed Opt"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3343","DOI":"10.1182\/blood-2010-07-296970","type":"journal-article","article-title":"Neutrophil survival and c-kit+-progenitor proliferation in Staphylococcus aureus-infected skin wounds promote resolution","volume":"117","author":"Kim","year":"2011","unstructured":"Kim, M. H. et al. Neutrophil survival and c-kit+-progenitor proliferation in Staphylococcus aureus-infected skin wounds promote resolution. Blood. 117, 3343-3352, DOI: blood-2010-07-296970 [pii];10.1182\/blood-2010-07-296970 [doi] (2011).","journal-title":"Blood"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"182","DOI":"10.2106\/JBJS.K.01262","type":"journal-article","article-title":"What's new in adult reconstructive knee surgery","volume":"94","author":"Deirmengian","year":"2012","unstructured":"Deirmengian, C. A., & Lonner, J. H. What's new in adult reconstructive knee surgery. J. Bone Joint Surg. Am. 94, 182-188, DOI: 10.2106\/JBJS.K.01262 [doi] (2012).","journal-title":"J Bone Joint Surg Am"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/nmat3074","type":"journal-article","article-title":"Maltodextrin-based imaging probes detect bacteria in vivo with high sensitivity and specificity","volume":"10","author":"Ning","year":"2011","unstructured":"Ning, X. et al. Maltodextrin-based imaging probes detect bacteria in vivo with high sensitivity and specificity. Nat. Mater. 10, 602-607, DOI: nmat3074 [pii];10.1038\/nmat3074 [doi] (2011).","journal-title":"Nat Mater"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1038\/nm.2423","type":"journal-article","article-title":"In vivo detection of Staphylococcus aureus endocarditis by targeting pathogen-specific prothrombin activation","volume":"17","author":"Panizzi","year":"2011","unstructured":"Panizzi, P. et al. In vivo detection of Staphylococcus aureus endocarditis by targeting pathogen-specific prothrombin activation. Nat. Med. 17, 1142-1146, DOI: nm.2423 [pii];10.1038\/nm.2423 [doi] (2011).","journal-title":"Nat Med"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2584","DOI":"10.1038\/ncomms3584","type":"journal-article","article-title":"Realtime in vivo imaging of invasive and biomaterial associated bacterial infections using fluorescently labelled vancomycin","volume":"4","author":"van Oosten","year":"2013","unstructured":"van Oosten, M., et al. Real-time in vivo imaging of invasive- and biomaterial-associated bacterial infections using fluorescently labelled vancomycin. Nat. Commun. 4, 2584, DOI: ncomms3584 [pii];10.1038\/ncomms3584 [doi] (2013).","journal-title":"Nat Commun"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"12239","DOI":"10.1073\/pnas.1000643107","type":"journal-article","article-title":"Imaging tuberculosis with endogenous beta lactamase reporter enzyme fluorescence in live mice","volume":"107","author":"Kong","year":"2010","unstructured":"Kong, Y. et al. Imaging tuberculosis with endogenous beta-lactamase reporter enzyme fluorescence in live mice. Proc. Natl. Acad. Sci. U. S. A. 107, 12239-12244, DOI: 1000643107 [pii];10.1073\/pnas.1000643107 [doi] (2010).","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/51612\/combined-vivo-optical-ct-imaging-to-monitor-infection-inflammation","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:02:29Z","timestamp":1792688549000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/51612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,16]]},"references-count":28,"journal-issue":{"issue":"92","published-online":{"date-parts":[[2014]]}},"URL":"https:\/\/doi.org\/10.3791\/51612","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,16]]}}}