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Alcohol Clin Exp Res. 31, 1252-1258, doi:10.1111\/j.1530-0277.2007.00422.x (2007).","journal-title":"Alcohol Clin Exp Res"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.alcohol.2007.12.004","type":"journal-article","article-title":"Maternal adrenocorticotropin, cortisol, and thyroid hormone responses to all three-trimester equivalent repeated binge alcohol exposure: ovine model","volume":"42","author":"Ramadoss","year":"2008","unstructured":"Ramadoss, J., Tress, U., Chen, W. J., & Cudd, T. A. Maternal adrenocorticotropin, cortisol, and thyroid hormone responses to all three-trimester equivalent repeated binge alcohol exposure: ovine model. Alcohol. 42, 199-205, doi:10.1016\/j.alcohol.2007.12.004 (2008).","journal-title":"Alcohol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S0892-0362(02)00354-9","type":"journal-article","article-title":"Brain growth spurt-prenatal ethanol exposure and the guinea pig hippocampal glutamate signaling system","volume":"25","author":"Byrnes","year":"2003","unstructured":"Byrnes, M. L., Reynolds, J. N., & Brien, J. F. Brain growth spurt-prenatal ethanol exposure and the guinea pig hippocampal glutamate signaling system. Neurotoxicol Teratol. 25, 303-310 (2003).","journal-title":"Neurotoxicol Teratol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0741-8329(93)90089-7","type":"journal-article","article-title":"Dose-dependent effects of prenatal ethanol exposure in the guinea pig","volume":"10","author":"Catlin","year":"1993","unstructured":"Catlin, M. C., Abdollah, S., & Brien, J. F. Dose-dependent effects of prenatal ethanol exposure in the guinea pig. Alcohol. 10, 109-115 (1993).","journal-title":"Alcohol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0736-5748(92)90059-9","type":"journal-article","article-title":"Effects of combined pre- and postnatal ethanol exposure (three trimester equivalency) on glial cell development in rat optic nerve","volume":"10","author":"Phillips","year":"1992","unstructured":"Phillips, D. E., & Krueger, S. K. Effects of combined pre- and postnatal ethanol exposure (three trimester equivalency) on glial cell development in rat optic nerve. 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J Neural Transm. 106, 773-786 (1999).","journal-title":"J Neural Transm"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.brainres.2011.01.116","type":"journal-article","article-title":"Altered adult hippocampal neuronal maturation in a rat model of fetal alcohol syndrome","volume":"1384","author":"Gil-Mohapel","year":"2011","unstructured":"Gil-Mohapel, J. et al. Altered adult hippocampal neuronal maturation in a rat model of fetal alcohol syndrome. Brain Res. 1384, 29-41, doi:10.1016\/j.brainres.2011.01.116 (2011).","journal-title":"Brain Res"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.bbr.2006.07.012","type":"journal-article","article-title":"Adult rat's offspring of alcoholic mothers are impaired on spatial learning and object recognition in the Can test","volume":"174","author":"Popovic","year":"2006","unstructured":"Popovic, M., Caballero-Bleda, M., & Guerri, C. 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Life Sci. 38, 1543-1556 (1986).","journal-title":"Life Sci"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s00213-007-0767-2","type":"journal-article","article-title":"Combined exposure to nicotine and ethanol throughout full gestation results in enhanced acquisition of nicotine self-administration in young adult rat offspring","volume":"193","author":"Matta","year":"2007","unstructured":"Matta, S. G., & Elberger, A. J. Combined exposure to nicotine and ethanol throughout full gestation results in enhanced acquisition of nicotine self-administration in young adult rat offspring. Psychopharmacology (Berl). 193, 199-213, doi:10.1007\/s00213-007-0767-2 (2007).","journal-title":"Psychopharmacology (Berl"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1080\/13556210410001717006","type":"journal-article","article-title":"Fetal alcohol syndrome at the cellular level)","volume":"9","author":"Olney","year":"2004","unstructured":"Olney, J. W. Fetal alcohol syndrome at the cellular level. Addict Biol. 9, 137-149; discussion 151, doi:10.1080\/13556210410001717006 (2004).","journal-title":"Addict Biol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1177\/15353702-0323006-04","type":"journal-article","article-title":"Genesis of alcohol-induced craniofacial dysmorphism","volume":"230","author":"Sulik","year":"2005","unstructured":"Sulik, K. K. Genesis of alcohol-induced craniofacial dysmorphism. Exp Biol Med (Maywood). 230, 366-375 (2005).","journal-title":"Exp Biol Med (Maywood)"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/laban0510-149","type":"journal-article","article-title":"Modifying a displacement pump for oral gavage dosing of solution and suspension preparations to adult and neonatal mice)","volume":"39","author":"Lewis","year":"2010","unstructured":"Lewis, S. M. et al. Modifying a displacement pump for oral gavage dosing of solution and suspension preparations to adult and neonatal mice. Lab Anim (NY). 39, 149-154, doi:10.1038\/laban0510-149 (2010).","journal-title":"Lab Anim (NY)"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.neulet.2009.03.034","type":"journal-article","article-title":"Effects of maternal alcohol consumption during breastfeeding on motor and cerebellar Purkinje cells behavior in mice","volume":"455","author":"Cebolla","year":"2009","unstructured":"Cebolla, A. M. et al. Effects of maternal alcohol consumption during breastfeeding on motor and cerebellar Purkinje cells behavior in mice. Neurosci Lett. 455, 4-7, doi:10.1016\/j.neulet.2009.03.034 (2009).","journal-title":"Neurosci Lett"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1111\/j.1530-0277.1993.tb00731.x","type":"journal-article","article-title":"Repeated episodes of ethanol withdrawal potentiate the severity of subsequent withdrawal seizures: an animal model of alcohol withdrawal &#34;kindling&#34","volume":"17","author":"Becker","year":"1993","unstructured":"Becker, H. C., & Hale, R. L. Repeated episodes of ethanol withdrawal potentiate the severity of subsequent withdrawal seizures: an animal model of alcohol withdrawal \"kindling\". Alcohol Clin. Exp. Res. 17, 94-98 (1993).","journal-title":"Alcohol Clin. Exp. Res"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.alcohol.2006.11.001","type":"journal-article","article-title":"Immature hippocampal neuronal networks do not develop tolerance to the excitatory actions of ethanol","volume":"40","author":"Galindo","year":"2006","unstructured":"Galindo, R., & Valenzuela, C. F. Immature hippocampal neuronal networks do not develop tolerance to the excitatory actions of ethanol. Alcohol. 40, 111-118, doi:10.1016\/j.alcohol.2006.11.001 (2006).","journal-title":"Alcohol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"7368","DOI":"10.1523\/JNEUROSCI.5746-12.2013","type":"journal-article","article-title":"Modeling transformations of neurodevelopmental sequences across mammalian species","volume":"33","author":"Workman","year":"2013","unstructured":"Workman, A.D., Charvet, C.J., Clancy, B., Darlington, R.B., Finlay, B.L. Modeling transformations of neurodevelopmental sequences across mammalian species. J Neurosci. 33, 7368-7383 (2013).","journal-title":"J Neurosci"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0741-8329(97)87949-9","type":"journal-article","article-title":"Repeated ethanol withdrawal experience increases the severity and duration of subsequent withdrawal seizures in mice","volume":"14","author":"Becker","year":"1997","unstructured":"Becker, H. C., Diaz-Granados, J. L., & Weathersby, R. T. Repeated ethanol withdrawal experience increases the severity and duration of subsequent withdrawal seizures in mice. Alcohol. 14, 319-326 (1997).","journal-title":"Alcohol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0890-6238(93)90234-X","type":"journal-article","article-title":"Effects of prenatal alcohol exposure in mice: influence of an ADH inhibitor and a chronic inhalation study","volume":"7","author":"Ukita","year":"1993","unstructured":"Ukita, K., Fukui, Y., & Shiota, K. Effects of prenatal alcohol exposure in mice: influence of an ADH inhibitor and a chronic inhalation study. Reprod Toxicol. 7, 273-281 (1993).","journal-title":"Reprod Toxicol"},{"key":"ref52","first-page":"65","type":"journal-article","article-title":"Influence of pyrazole, an inhibitor of alcohol dehydrogenase on the prenatal toxicity of ethanol in the rat","volume":"26","author":"Varma","year":"1979","unstructured":"Varma, P. K., & Persaud, T. V. Influence of pyrazole, an inhibitor of alcohol dehydrogenase on the prenatal toxicity of ethanol in the rat. Res Commun Chem Pathol Pharmacol. 26, 65-73 (1979).","journal-title":"Res Commun Chem Pathol Pharmacol"},{"key":"ref53","first-page":"42","type":"journal-article","article-title":"Laboratory routines cause animal stress","volume":"43","author":"Balcombe","year":"2004","unstructured":"Balcombe, J. P., Barnard, N. D., & Sandusky, C. Laboratory routines cause animal stress. Contemp Top Lab Anim Sci. 43, 42-51 (2004).","journal-title":"Contemp Top Lab Anim Sci"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.pbb.2008.02.017","type":"journal-article","article-title":"Acute sensitivity and acute tolerance to ethanol in preweanling rats with or without prenatal experience with the drug","volume":"89","author":"Arias","year":"2008","unstructured":"Arias, C., Molina, J. C., Mlewski, E. C., Pautassi, R. M., & Spear, N. Acute sensitivity and acute tolerance to ethanol in preweanling rats with or without prenatal experience with the drug. Pharmacol Biochem Behav. 89, 608-622, doi:10.1016\/j.pbb.2008.02.017 (2008).","journal-title":"Pharmacol Biochem Behav"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/j.1530-0277.2006.00009.x","type":"journal-article","article-title":"Prenatal ethanol exposure increases ethanol reinforcement in neonatal rats","volume":"30","author":"Nizhnikov","year":"2006","unstructured":"Nizhnikov, M. E., Molina, J. C., Varlinskaya, E. I., & Spear, N. E. Prenatal ethanol exposure increases ethanol reinforcement in neonatal rats. Alcohol Clin Exp Res. 30, 34-45, doi:10.1111\/j.1530-0277.2006.00009.x (2006).","journal-title":"Alcohol Clin Exp Res"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/51839\/construction-vapor-chambers-used-to-expose-mice-to-alcohol-during","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:27:10Z","timestamp":1792690030000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/51839"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,13]]},"references-count":55,"journal-issue":{"issue":"89","published-online":{"date-parts":[[2014]]}},"URL":"https:\/\/doi.org\/10.3791\/51839","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,13]]}}}