{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:40:24Z","timestamp":1792690824964,"version":"4.5.0"},"reference-count":14,"publisher":"MyJove Corporation","issue":"93","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/52121","type":"journal-article","created":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T10:25:20Z","timestamp":1417170320000},"source":"Crossref","is-referenced-by-count":0,"title":["<em>In Vitro<\/em> Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection"],"prefix":"10.3791","author":[{"given":"Guorui","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa C.","family":"Whiteman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason A.","family":"Wicker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan D.T.","family":"Barrett","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2014,11,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/S1473-3099(02)00368-7","type":"journal-article","article-title":"West Nile virus","volume":"2","author":"Campbell","year":"2002","unstructured":"Campbell, G. L., Marfin, A. A., Lanciotti, R. S., & Gubler, D. J. West Nile virus. Lancet Infect. Dis. 2, 519-529. (2002).","journal-title":"Lancet Infect. Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"4853","DOI":"10.1016\/j.vaccine.2011.04.057","type":"journal-article","article-title":"Immune responses to an attenuated West Nile virus NS4B-P38G mutant strain","volume":"29","author":"Welte","year":"2011","unstructured":"Welte, T. et al. Immune responses to an attenuated West Nile virus NS4B-P38G mutant strain. Vaccine. 29, 4853-4861, doi:S0264-410X(11)00602-5 [pii] 10.1016\/j.vaccine.2011.04.057 (2011).","journal-title":"Vaccine"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1038\/ni1112","type":"journal-article","article-title":"Toll-like receptor control of the adaptive immune responses","volume":"5","author":"Iwasaki","year":"2004","unstructured":"Iwasaki, A., & Medzhitov, R. Toll-like receptor control of the adaptive immune responses. Nat. Immunol. 5, 987-995 (2004).","journal-title":"Nat. Immunol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0165-2478(02)00228-6","type":"journal-article","article-title":"Recognition of pathogen-associated molecular patterns by TLR family","volume":"85","author":"Akira","year":"2003","unstructured":"Akira, S., & Hemmi, H. Recognition of pathogen-associated molecular patterns by TLR family. Immunol. Lett. 85, 85-95 (2003).","journal-title":"Immunol. Lett"},{"issue":"38","key":"ref5","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.1016\/j.vaccine.2013.06.093","type":"journal-article","article-title":"A West Nile virus NS4B-P38G mutant strain induces adaptive immunity via TLR7-MyD88-dependent and independent signaling pathways","volume":"31","author":"Xie","year":"2013","unstructured":"Xie, G. et al. A West Nile virus NS4B-P38G mutant strain induces adaptive immunity via TLR7-MyD88-dependent and independent signaling pathways. Vaccine. 31 (38), 4143-51 doi:S0264-410X(13)00903-1 [pii] 10.1016\/j.vaccine.2013.06.093 (2013).","journal-title":"Vaccine"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/j.1574-695X.2010.00663.x","type":"journal-article","article-title":"gammadelta T cells promote the maturation of dendritic cells during West Nile virus infection","volume":"59","author":"Fang","year":"2010","unstructured":"Fang, H. et al. gammadelta T cells promote the maturation of dendritic cells during West Nile virus infection. FEMS Immunol. Med. Microbiol. 59, 71-80, doi:FIM663 [pii] 10.1111\/j.1574-695X.2010.00663.x (2010).","journal-title":"FEMS Immunol. Med. Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"13640","DOI":"10.1128\/JVI.00857-07","type":"journal-article","article-title":"Differential activation of human monocyte-derived and plasmacytoid dendritic cells by West Nile virus generated in different host cells","volume":"81","author":"Silva","year":"2007","unstructured":"Silva, M. C., Guerrero-Plata, A., Gilfoy, F. D., Garofalo, R. P., & Mason, P. W. Differential activation of human monocyte-derived and plasmacytoid dendritic cells by West Nile virus generated in different host cells. J. Virol. 81, 13640-13648 (2007).","journal-title":"J. Virol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1128\/JVI.80.1.119-129.2006","type":"journal-article","article-title":"CD8+ T Cells Require Perforin To Clear West Nile Virus from Infected Neurons","volume":"80","author":"Shrestha","year":"2006","unstructured":"Shrestha, B., Samuel, M. A., & Diamond, M. S. CD8+ T Cells Require Perforin To Clear West Nile Virus from Infected Neurons. J. Virol. 80, 119-129 (2006).","journal-title":"J. Virol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"13323","DOI":"10.1128\/JVI.77.24.13323-13334.2003","type":"journal-article","article-title":"CD8+ T cells mediate recovery and immunopathology in West Nile virus encephalitis","volume":"77","author":"Wang","year":"2003","unstructured":"Wang, Y., Lobigs, M., Lee, E., & Mullbacher, A. CD8+ T cells mediate recovery and immunopathology in West Nile virus encephalitis. J. Virol. 77, 13323-13334 (2003).","journal-title":"J. Virol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1586\/erv.10.53","type":"journal-article","article-title":"Methods to measure T-cell responses","volume":"9","author":"Plebanski","year":"2010","unstructured":"Plebanski, M., Katsara, M., Sheng, K. C., Xiang, S. D., & Apostolopoulos, V. Methods to measure T-cell responses. Expert Rev. Vaccines. 9, 595-600, doi:10.1586\/erv.10.53 (2010).","journal-title":"Expert Rev. Vaccines"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0022-1759(00)00231-3","type":"journal-article","article-title":"Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution","volume":"243","author":"Lyons","year":"2000","unstructured":"Lyons, A. B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J. Immunol. Methods. 243, 147-154, doi:S0022-1759(00)00231-3 [pii] (2000).","journal-title":"J. Immunol. Methods"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1159\/000084603","type":"journal-article","article-title":"Inactivation of Hantaan virus-containing samples for subsequent investigations outside biosafety level 3 facilities","volume":"48","author":"Kraus","year":"2005","unstructured":"Kraus, A. A., Priemer, C., Heider, H., Kruger, D. H., & Ulrich, R. Inactivation of Hantaan virus-containing samples for subsequent investigations outside biosafety level 3 facilities. Intervirology. 48, 255-261, doi:84603 [pii] 10.1159\/000084603 (2005).","journal-title":"Intervirology"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1371\/journal.pntd.0002275","type":"journal-article","article-title":"A hamster-derived west nile virus isolate induces persistent renal infection in mice","volume":"7","author":"Saxena","year":"2013","unstructured":"Saxena, V. et al. A hamster-derived west nile virus isolate induces persistent renal infection in mice. PLoS Negl. Trop. Dis. 7, e2275, doi:10.1371\/journal.pntd.0002275 PNTD-D-13-00050 [pii] (2013).","journal-title":"PLoS Negl. Trop. Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/j.1574-695X.2011.00840.x","type":"journal-article","article-title":"Vgamma4+ T cells regulate host immune response to West Nile virus infection","volume":"63","author":"Welte","year":"2011","unstructured":"Welte, T. et al. Vgamma4+ T cells regulate host immune response to West Nile virus infection. FEMS Immunol. Med. Microbiol. 63, 183-192, doi:10.1111\/j.1574-695X.2011.00840.x (2011).","journal-title":"FEMS Immunol. Med. Microbiol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/52121\/in-vitro-analysis-myd88-mediated-cellular-immune-response-to-west","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T16:52:40Z","timestamp":1792687960000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/52121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,27]]},"references-count":14,"journal-issue":{"issue":"93","published-online":{"date-parts":[[2014]]}},"URL":"https:\/\/doi.org\/10.3791\/52121","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,27]]}}}