{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:39:08Z","timestamp":1792690748253,"version":"4.5.0"},"reference-count":27,"publisher":"MyJove Corporation","issue":"94","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/52130","type":"journal-article","created":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T18:59:58Z","timestamp":1419965998000},"source":"Crossref","is-referenced-by-count":1,"title":["Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an <em>Ex Vivo<\/em> Autoperfused Microflow Chamber Assay"],"prefix":"10.3791","author":[{"given":"Lama","family":"Mulki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Harry","family":"Sweigard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kip M.","family":"Connor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2014,12,30]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/S0300-8932(09)71035-8","type":"journal-article","article-title":"Molecular basis of leukocyte-endothelium interactions during the inflammatory response","volume":"62","author":"Barreiro","year":"2009","unstructured":"Barreiro, O., & Sanchez-Madrid, F. Molecular basis of leukocyte-endothelium interactions during the inflammatory response. Revista Espanola de Cardiologia. 62, 552-562 (2009).","journal-title":"Revista Espanola de Cardiologia"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S1471-4906(03)00117-0","type":"journal-article","article-title":"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response","volume":"24","author":"Muller","year":"2003","unstructured":"Muller, W. A. Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response. Trends in Immunology. 24, 327-334 (2003).","journal-title":"Trends in Immunology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.ceb.2008.04.003","type":"journal-article","article-title":"Cells on the run: shear-regulated integrin activation in leukocyte rolling and arrest on endothelial cells","volume":"20","author":"Alon","year":"2008","unstructured":"Alon, R., & Ley, K. Cells on the run: shear-regulated integrin activation in leukocyte rolling and arrest on endothelial cells. Current Opinion in Cell Biology. 20, 525-532, doi: 10.1016\/j.ceb.2008.04.003 (2008).","journal-title":"Current Opinion in Cell Biology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"32506","DOI":"10.1074\/jbc.273.49.32506","type":"journal-article","article-title":"Affinity and kinetic analysis of P-selectin binding to P-selectin glycoprotein ligand-1","volume":"273","author":"Mehta","year":"1998","unstructured":"Mehta, P., Cummings, R. D., & McEver, R. P. Affinity and kinetic analysis of P-selectin binding to P-selectin glycoprotein ligand-1. The Journal of Biological Chemistry. 273, 32506-32513 (1998).","journal-title":"The Journal of Biological Chemistry"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1074\/jbc.273.2.763","type":"journal-article","article-title":"Affinity and kinetic analysis of L-selectin (CD62L) binding to glycosylation-dependent cell-adhesion molecule-1","volume":"273","author":"Nicholson","year":"1998","unstructured":"Nicholson, M. W., Barclay, A. N., Singer, M. S., Rosen, S. D., & van der Merwe, P. A. Affinity and kinetic analysis of L-selectin (CD62L) binding to glycosylation-dependent cell-adhesion molecule-1. The Journal of Biological Chemistry. 273, 763-770 (1998).","journal-title":"The Journal of Biological Chemistry"},{"issue":"22","key":"ref6","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.1242\/jcs.110.22.2807","type":"journal-article","article-title":"Changes in the distribution of LFA-1, catenins, and F-actin during transendothelial migration of monocytes in culture","volume":"110","author":"Sandig","year":"1997","unstructured":"Sandig, M., Negrou, E., & Rogers, K. A. Changes in the distribution of LFA-1, catenins, and F-actin during transendothelial migration of monocytes in culture. Journal of Cell Science. 110 (22), 2807-2818 (1997).","journal-title":"Journal of Cell Science"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1084\/jem.20040965","type":"journal-article","article-title":"Coordinated redistribution of leukocyte LFA-1 and endothelial cell ICAM-1 accompany neutrophil transmigration","volume":"200","author":"Shaw","year":"2004","unstructured":"Shaw, S. K. et al. Coordinated redistribution of leukocyte LFA-1 and endothelial cell ICAM-1 accompany neutrophil transmigration. The Journal of Experimental Medicine. 200, 1571-1580, doi: 10.1084\/jem.20040965 (2004).","journal-title":"The Journal of Experimental Medicine"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1182\/blood-2006-09-047431","type":"journal-article","article-title":"JAM-A mediates neutrophil transmigration in a stimulus-specific manner in vivo: evidence for sequential roles for JAM-A and PECAM-1 in neutrophil transmigration","volume":"110","author":"Woodfin","year":"2007","unstructured":"Woodfin, A. et al. JAM-A mediates neutrophil transmigration in a stimulus-specific manner in vivo: evidence for sequential roles for JAM-A and PECAM-1 in neutrophil transmigration. Blood. 110, 1848-1856, doi: 10.1182\/blood-2006-09-047431 (2007).","journal-title":"Blood"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0021-9150(03)00097-2","type":"journal-article","article-title":"Adhesion molecules and atherosclerosis","volume":"170","author":"Blankenberg","year":"2003","unstructured":"Blankenberg, S., Barbaux, S., & Tiret, L. Adhesion molecules and atherosclerosis. Atherosclerosis. 170, 191-203 (2003).","journal-title":"Atherosclerosis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"546786","DOI":"10.1155\/2012\/546786","type":"journal-article","article-title":"Mechanism of inflammation in age-related macular degeneration","volume":"2012","author":"Parmeggiani","year":"2012","unstructured":"Parmeggiani, F. et al. Mechanism of inflammation in age-related macular degeneration. Mediators of Inflammation. 2012, 546786, doi: 10.1155\/2012\/546786 (2012).","journal-title":"Mediators of Inflammation"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.preteyeres.2008.10.001","type":"journal-article","article-title":"Molecular pathology of age-related macular degeneration","volume":"28","author":"Ding","year":"2009","unstructured":"Ding, X., Patel, M., & Chan, C. C. Molecular pathology of age-related macular degeneration. Progress in Retinal and Eye Research. 28, 1-18, doi: 10.1016\/j.preteyeres.2008.10.001 (2009).","journal-title":"Progress in Retinal and Eye Research"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.preteyeres.2009.06.001","type":"journal-article","article-title":"Para-inflammation in the aging retina","volume":"28","author":"Xu","year":"2009","unstructured":"Xu, H., Chen, M., & Forrester, J. V. Para-inflammation in the aging retina. Progress in Retinal and Eye Research. 28, 348-368, doi: 10.1016\/j.preteyeres.2009.06.001 (2009).","journal-title":"Progress in Retinal and Eye Research"},{"key":"ref13","type":"book","article-title":"Drug Class Review: Nonsteroidal Antiinflammatory Drugs (NSAIDs): Final Update 4 Report","volume-title":"Drug Class Reviews","author":"Peterson","year":"2010","unstructured":"Peterson, K. et al. in Drug Class Review: Nonsteroidal Antiinflammatory Drugs (NSAIDs): Final Update 4 ReportDrug Class Reviews (2010)."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.tips.2003.10.004","type":"journal-article","article-title":"Leukocyte and endothelial cell adhesion molecules as targets for therapeutic interventions in inflammatory disease","volume":"24","author":"Ulbrich","year":"2003","unstructured":"Ulbrich, H., Eriksson, E. E., & Lindbom, L. Leukocyte and endothelial cell adhesion molecules as targets for therapeutic interventions in inflammatory disease. Trends in Pharmacological Sciences. 24, 640-647, doi: 10.1016\/j.tips.2003.10.004 (2003).","journal-title":"Trends in Pharmacological Sciences"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1084\/jem.144.3.828","type":"journal-article","article-title":"Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules","volume":"144","author":"Stamper","year":"1976","unstructured":"Stamper, H. B., Jr., & Woodruff, J. J. Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules. The Journal of Experimental Medicine. 144, 828-833 (1976).","journal-title":"The Journal of Experimental Medicine"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1182\/blood.V70.5.1284.1284","type":"journal-article","article-title":"Effect of flow on polymorphonuclear leukocyte\/endothelial cell adhesion","volume":"70","author":"Lawrence","year":"1987","unstructured":"Lawrence, M. B., McIntire, L. V., & Eskin, S. G. Effect of flow on polymorphonuclear leukocyte\/endothelial cell adhesion. Blood. 70, 1284-1290 (1987).","journal-title":"Blood"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1046\/j.1365-2141.2001.02544.x","type":"journal-article","article-title":"Characteristics of leucocyte adhesion directly observed in flowing whole blood in vitro","volume":"112","author":"Abbitt","year":"2001","unstructured":"Abbitt, K. B., & Nash, G. B. Characteristics of leucocyte adhesion directly observed in flowing whole blood in vitro. British Journal of Haematology. 112, 55-63 (2001).","journal-title":"British Journal of Haematology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1189\/jlb.1003483","type":"journal-article","article-title":"Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin","volume":"76","author":"Smith","year":"2004","unstructured":"Smith, M. L., Sperandio, M., Galkina, E. V., & Ley, K. Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin. Journal of Leukocyte Biology. 76, 985-993, doi: 10.1189\/jlb.1003483 (2004).","journal-title":"Journal of Leukocyte Biology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"388","DOI":"10.2144\/01302rr02","type":"journal-article","article-title":"Lateral view flow system for studies of cell adhesion and deformation under flow conditions","volume":"30","author":"Yuan","year":"2001","unstructured":"Yuan, J., Melder, R. J., Jain, R. K., & Munn, L. L. Lateral view flow system for studies of cell adhesion and deformation under flow conditions. BioTechniques. 30, 388-394 (2001).","journal-title":"BioTechniques"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/4734","type":"journal-article","article-title":"Autoimmune T cells protect neurons from secondary degeneration after central nervous system axotomy","volume":"5","author":"Moalem","year":"1999","unstructured":"Moalem, G. et al. Autoimmune T cells protect neurons from secondary degeneration after central nervous system axotomy. Nature Medicine. 5, 49-55, doi: 10.1038\/4734 (1999).","journal-title":"Nature Medicine"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1152\/ajpcell.00500.2003","type":"journal-article","article-title":"A novel mouse-driven ex vivo flow chamber for the study of leukocyte and platelet function","volume":"286","author":"Hafezi-Moghadam","year":"2004","unstructured":"Hafezi-Moghadam, A., Thomas, K. L., & Cornelssen, C. A novel mouse-driven ex vivo flow chamber for the study of leukocyte and platelet function. American Journal of Physiology. Cell Physiology. 286, C876-892, doi: 10.1152\/ajpcell.00500.2003 (2004).","journal-title":"American Journal of Physiology. Cell Physiology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1096\/fj.08-118760","type":"journal-article","article-title":"Surprising up-regulation of P-selectin glycoprotein ligand-1 (PSGL-1) in endotoxin-induced uveitis","volume":"23","author":"Almulki","year":"2009","unstructured":"Almulki, L. et al. Surprising up-regulation of P-selectin glycoprotein ligand-1 (PSGL-1) in endotoxin-induced uveitis. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology. 23, 929-939, doi: 10.1096\/fj.08-118760 (2009).","journal-title":"FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","type":"journal-article","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","unstructured":"Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nature Methods. 9, 676-682, doi: 10.1038\/nmeth.2019 (2012).","journal-title":"Nature Methods"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nm0798-814","type":"journal-article","article-title":"Implantation of stimulated homologous macrophages results in partial recovery of paraplegic rats","volume":"4","author":"Rapalino","year":"1998","unstructured":"Rapalino, O. et al. Implantation of stimulated homologous macrophages results in partial recovery of paraplegic rats. Nature Medicine. 4, 814-821 (1998).","journal-title":"Nature Medicine"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3740","DOI":"10.1523\/JNEUROSCI.21-11-03740.2001","type":"journal-article","article-title":"Protective autoimmunity is a physiological response to CNS trauma","volume":"21","author":"Yoles","year":"2001","unstructured":"Yoles, E. et al. Protective autoimmunity is a physiological response to CNS trauma. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. 21, 3740-3748 (2001).","journal-title":"The Journal of Neuroscience : the Official Journal of the Society for Neuroscience"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1182\/blood.V62.4.889.889","type":"journal-article","article-title":"Arachidonic acid metabolism and the adhesion of human polymorphonuclear leukocytes to cultured vascular endothelial cells","volume":"62","author":"Buchanan","year":"1983","unstructured":"Buchanan, M. R., Vazquez, M. J., & Gimbrone, M. A., Jr. Arachidonic acid metabolism and the adhesion of human polymorphonuclear leukocytes to cultured vascular endothelial cells. Blood. 62, 889-895 (1983).","journal-title":"Blood"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"10260","DOI":"10.1158\/0008-5472.CAN-07-1204","type":"journal-article","article-title":"Manganese superoxide dismutase enhances the invasive and migratory activity of tumor cells","volume":"67","author":"Connor","year":"2007","unstructured":"Connor, K. M. et al. Manganese superoxide dismutase enhances the invasive and migratory activity of tumor cells. Cancer Res. 67, 10260-10267, doi: 10.1158\/0008-5472.CAN-07-1204 (2007).","journal-title":"Cancer Res"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/52130\/assessing-leukocyte-endothelial-interactions-under-flow-conditions-an","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T16:43:27Z","timestamp":1792687407000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/52130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,30]]},"references-count":27,"journal-issue":{"issue":"94","published-online":{"date-parts":[[2014]]}},"URL":"https:\/\/doi.org\/10.3791\/52130","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,30]]}}}