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Biochimica et Biophysica Acta (BBA) - General Subjects. 1201, (2), 186-192 (1994).","journal-title":"Biochimica et Biophysica Acta (BBA) - General Subjects"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1002\/jcp.22167","type":"journal-article","article-title":"Post-transcriptional regulation of \u03b1-smooth muscle actin determines the contractile phenotype of Dupuytren's nodular cells","volume":"224","author":"Verjee","year":"2010","unstructured":"Verjee, L. S., Midwood, K., Davidson, D., Eastwood, M., Nanchahal, J. Post-transcriptional regulation of \u03b1-smooth muscle actin determines the contractile phenotype of Dupuytren's nodular cells. 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