{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T04:02:44Z","timestamp":1793937764770,"version":"4.8.0"},"reference-count":31,"publisher":"MyJove Corporation","issue":"116","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/52940","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T20:00:07Z","timestamp":1476302407000},"source":"Crossref","is-referenced-by-count":7,"title":["Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis"],"prefix":"10.3791","author":[{"given":"Mathieu","family":"Manassero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Decambron","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bui Truong","family":"Huu Thong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Viateau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morad","family":"Bensidhoum","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Petite","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2016,10,12]]},"reference":[{"issue":"72","key":"ref1","type":"journal-article","article-title":"Refining animal models in fracture research: seeking consensus in optimising both animal welfare and scientific validity for appropriate biomedical use","volume":"8","author":"Auer","year":"2007","unstructured":"Auer, J. A. et al. Refining animal models in fracture research: seeking consensus in optimising both animal welfare and scientific validity for appropriate biomedical use. BMC Musculoskelet Disord. 8), 72 (2007).","journal-title":"BMC Musculoskelet Disord"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.bone.2011.07.007","type":"journal-article","article-title":"Small animal bone healing models: standards, tips, and pitfalls results of a consensus meeting","volume":"49","author":"Histing","year":"2011","unstructured":"Histing, T. et al. Small animal bone healing models: standards, tips, and pitfalls results of a consensus meeting. Bone. 49 (4), 591-599 (2011).","journal-title":"Bone"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1089\/ten.teb.2009.0224","type":"journal-article","article-title":"Long bone defect models for tissue engineering applications: criteria for choice","volume":"16","author":"Horner","year":"2010","unstructured":"Horner, E. A. et al. Long bone defect models for tissue engineering applications: criteria for choice. Tissue Eng. Part B Rev. 16 (2), 263-271 (2010).","journal-title":"Tissue Eng. Part B Rev"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1089\/ten.tec.2010.0501","type":"journal-article","article-title":"A model for tissue engineering applications: femoral critical size defect in immunodeficient mice","volume":"17","author":"Srouji","year":"2011","unstructured":"Srouji, S. et al. A model for tissue engineering applications: femoral critical size defect in immunodeficient mice. Tissue Eng. Part C Methods. 17 (5), 597-606 (2011).","journal-title":"Tissue Eng. Part C Methods"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/S0736-0266(02)00017-7","type":"journal-article","article-title":"A model for intramembranous ossification during fracture healing","volume":"20","author":"Thompson","year":"2002","unstructured":"Thompson, Z., Miclau, T., Hu, D., Helms, J. A. A model for intramembranous ossification during fracture healing. J Orthop Res. 20 (5), 1091-1098 (2002).","journal-title":"J Orthop Res"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.bone.2013.02.002","type":"journal-article","article-title":"A review of mouse critical size defect models in weight bearing bones","volume":"55","author":"Harris","year":"2013","unstructured":"Harris, J. S., Bemenderfer, T. B., Wessel, A. R., Kacena, M. A. A review of mouse critical size defect models in weight bearing bones. Bone. 55 (1), 241-247 (2013).","journal-title":"Bone"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.jss.2009.11.713","type":"journal-article","article-title":"The LockingMouseNail--a new implant for standardized stable osteosynthesis in mice","volume":"169","author":"Garcia","year":"2011","unstructured":"Garcia, P. et al. The LockingMouseNail--a new implant for standardized stable osteosynthesis in mice. J. Surg. Res. 169 (2), 220-226 (2011).","journal-title":"J. Surg. Res"},{"issue":"10","key":"ref8","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/j.injury.2009.07.002","type":"journal-article","article-title":"Femoral non-union models in the mouse","volume":"41","author":"Garcia","year":"2010","unstructured":"Garcia, P., Histing, T., Holstein, J. H., Pohlemann, T., Menger, M. D. Femoral non-union models in the mouse. Injury. 41 (10), 1093-1094 (2010).","journal-title":"Injury"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jss.2007.09.013","type":"journal-article","article-title":"Development of a reliable non-union model in mice","volume":"147","author":"Garcia","year":"2008","unstructured":"Garcia, P. et al. Development of a reliable non-union model in mice. J. Surg. Res. 147 (1), 84-91 (2008).","journal-title":"J. Surg. Res"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-59745-285-4_74","type":"book","article-title":"Animal Models for bone tisue enginering purposes","volume-title":"Sourcebook of models for biomedical research","author":"Viateau","year":"2008","unstructured":"Viateau, V., Logeart-Avramoglou, D., Guillemin, G., Petite, H. Animal Models for bone tisue enginering purposes. In: Sourcebook of models for biomedical research. Conn, P.M., eds., Humana Press, 725-738 (2008)."},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1089\/ten.tec.2012.0256","type":"journal-article","article-title":"A novel murine femoral segmental critical-sized defect model stabilized by plate osteosynthesis for bone tissue engineering purposes","volume":"19","author":"Manassero","year":"2013","unstructured":"Manassero, M. et al. A novel murine femoral segmental critical-sized defect model stabilized by plate osteosynthesis for bone tissue engineering purposes. Tissue Eng. Part C Methods. 19 (4), 271-280 (2013).","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"S103","DOI":"10.1016\/j.injury.2009.10.044","type":"journal-article","article-title":"Internal fixator for use in the mouse","volume":"40 Suppl 4","author":"Matthys","year":"2009","unstructured":"Matthys, R., Perren, S. M. Internal fixator for use in the mouse. Injury. (40 Suppl 4), S103-109 (2009).","journal-title":"Injury"},{"issue":"19-20","key":"ref13","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1089\/ten.tea.2011.0690","type":"journal-article","article-title":"Ischemia is the prime but not the only cause of human multipotent stromal cell death in tissue-engineered constructs in vivo","volume":"18","author":"Becquart","year":"2012","unstructured":"Becquart, P. et al. Ischemia is the prime but not the only cause of human multipotent stromal cell death in tissue-engineered constructs in vivo. Tissue Eng. Part A. 18 (19-20), 2084-2094 (2012).","journal-title":"Tissue Eng. Part A"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1002\/stem.1299","type":"journal-article","article-title":"Proangiogenic and prosurvival functions of glucose in human mesenchymal stem cells upon transplantation","volume":"31","author":"Deschepper","year":"2013","unstructured":"Deschepper, M. et al. Proangiogenic and prosurvival functions of glucose in human mesenchymal stem cells upon transplantation. Stem Cells. 31 (3), 526-535 (2013).","journal-title":"Stem Cells"},{"issue":"10","key":"ref15","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.injury.2008.04.008","type":"journal-article","article-title":"Development of a femoral non-union model in the mouse","volume":"39","author":"Oetgen","year":"2008","unstructured":"Oetgen, M. E., Merrell, G. A., Troiano, N. W., Horowitz, M. C., Kacena, M. A. Development of a femoral non-union model in the mouse. Injury. 39 (10), 1119-1126 (2008).","journal-title":"Injury"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.jss.2013.02.041","type":"journal-article","article-title":"A murine femoral segmental defect model for bone tissue engineering using a novel rigid internal fixation system","volume":"183","author":"Liu","year":"2013","unstructured":"Liu, K. et al. A murine femoral segmental defect model for bone tissue engineering using a novel rigid internal fixation system. J Surg Res. 183 (2), 493-502 (2013).","journal-title":"J Surg Res"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"e7","DOI":"10.1016\/j.jss.2012.06.039","type":"journal-article","article-title":"Establishment of a femoral critical-size bone defect model in immunodeficient mice","volume":"181","author":"Zwingenberger","year":"2013","unstructured":"Zwingenberger, S. et al. Establishment of a femoral critical-size bone defect model in immunodeficient mice. J Surg Res. 181 (1), e7-e14 (2013).","journal-title":"J Surg Res"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/S0736-0266(03)00026-3","type":"journal-article","article-title":"An externally fixed femoral fracture model for mice","volume":"21","author":"Cheung","year":"2003","unstructured":"Cheung, K. M. et al. An externally fixed femoral fracture model for mice. J. Orthop Res. 21 (4), 685-690 (2003).","journal-title":"J. Orthop Res"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00223-009-9262-6","type":"journal-article","article-title":"Hyperhomocysteinemia is associated with impaired fracture healing in mice","volume":"85","author":"Claes","year":"2009","unstructured":"Claes, L. et al. Hyperhomocysteinemia is associated with impaired fracture healing in mice. Calcif. Tissue Int. 85 (1), 17-21 (2009).","journal-title":"Calcif. Tissue Int"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1089\/tec.2007.0234","type":"journal-article","article-title":"Validation of a femoral critical size defect model for orthotopic evaluation of bone healing: a biomechanical, veterinary and trauma surgical perspective","volume":"14","author":"Drosse","year":"2008","unstructured":"Drosse, I. et al. Validation of a femoral critical size defect model for orthotopic evaluation of bone healing: a biomechanical, veterinary and trauma surgical perspective. Tissue Eng. Part C Methods. 14 (1), 79-88 (2008).","journal-title":"Tissue Eng. Part C Methods"},{"issue":"5 Suppl","key":"ref21","doi-asserted-by":"crossref","first-page":"S31","DOI":"10.1097\/BOT.0b013e31819f27e5","type":"journal-article","article-title":"Advances in the establishment of defined mouse models for the study of fracture healing and bone regeneration","volume":"23","author":"Holstein","year":"2009","unstructured":"Holstein, J. H. et al. Advances in the establishment of defined mouse models for the study of fracture healing and bone regeneration. J. Orthop. Trauma. 23 (5 Suppl), S31-38 (2009).","journal-title":"J. Orthop. Trauma"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1097\/00005131-199601000-00005","type":"journal-article","article-title":"Evaluation of ground cortical autograft as a bone graft material in a new canine bilateral segmental long bone defect model","volume":"10","author":"Johnson","year":"1996","unstructured":"Johnson, K. D., August, A., Sciadini, M. F., Smith, C. Evaluation of ground cortical autograft as a bone graft material in a new canine bilateral segmental long bone defect model. J. Orthop. Trauma. 10 (1), 28-36 (1996).","journal-title":"J. Orthop. Trauma"},{"issue":"8","key":"ref23","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1097\/00005131-199711000-00002","type":"journal-article","article-title":"Regeneration of diaphyseal bone defects using resorbable poly(L\/DL-lactide) and poly(D-lactide) membranes in the Yucatan pig model","volume":"11","author":"Meinig","year":"1997","unstructured":"Meinig, R. P., Buesing, C. M., Helm, J., Gogolewski, S. Regeneration of diaphyseal bone defects using resorbable poly(L\/DL-lactide) and poly(D-lactide) membranes in the Yucatan pig model. J. Orthop. Trauma. 11 (8), 551-558 (1997).","journal-title":"J. Orthop. Trauma"},{"issue":"8","key":"ref24","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.2106\/00004623-198466080-00015","type":"journal-article","article-title":"Comparison of osteotomy healing under external fixation devices with different stiffness characteristics","volume":"66","author":"Wu","year":"1984","unstructured":"Wu, J. J., Shyr, H. S., Chao, E. Y., Kelly, P. J. Comparison of osteotomy healing under external fixation devices with different stiffness characteristics. J. Bone Joint Surg. Am. 66 (8), 1258-1264 (1984).","journal-title":"J. Bone Joint Surg. Am"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.jss.2014.05.037","type":"journal-article","article-title":"Establishment of a bilateral femoral large segmental bone defect mouse model potentially applicable to basic research in bone tissue engineering","volume":"192","author":"Xing","year":"2014","unstructured":"Xing, J. et al. Establishment of a bilateral femoral large segmental bone defect mouse model potentially applicable to basic research in bone tissue engineering. J. Surg. Res 192 (2), 454-463 (2014).","journal-title":"J. Surg. Res"},{"issue":"5-6","key":"ref26","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1089\/ten.tea.2015.0346","type":"journal-article","article-title":"Comparison of Survival and Osteogenic Ability of Human Mesenchymal Stem Cells in Orthotopic and Ectopic Sites in Mice","volume":"22","author":"Manassero","year":"2016","unstructured":"Manassero, M. et al. Comparison of Survival and Osteogenic Ability of Human Mesenchymal Stem Cells in Orthotopic and Ectopic Sites in Mice. Tissue Eng. Part A. 22 (5-6), 534-544 (2016).","journal-title":"Tissue Eng. Part A"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/0142-9612(91)90128-W","type":"journal-article","article-title":"Degradation of and tissue reaction to biodegradable poly(L-lactide) for use as internal fixation of fractures: a study in rats","volume":"12","author":"Bos","year":"1991","unstructured":"Bos, R. R. et al. Degradation of and tissue reaction to biodegradable poly(L-lactide) for use as internal fixation of fractures: a study in rats. Biomaterials. 12 (1), 32-36 (1991).","journal-title":"Biomaterials"},{"issue":"7","key":"ref28","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1002\/jor.20372","type":"journal-article","article-title":"Quantitative assessment of scaffold and growth factor-mediated repair of critically sized bone defects","volume":"25","author":"Oest","year":"2007","unstructured":"Oest, M. E., Dupont, K. M., Kong, H. J., Mooney, D. J., Guldberg, R. E. Quantitative assessment of scaffold and growth factor-mediated repair of critically sized bone defects. J.Orthop. Res. 25 (7), 941-950 (2007).","journal-title":"J.Orthop. Res"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.bone.2006.04.009","type":"journal-article","article-title":"Long-term tissue response to bioabsorbable poly-L-lactide and metallic screws: an experimental study","volume":"39","author":"Pihlajamaki","year":"2006","unstructured":"Pihlajamaki, H., Bostman, O., Tynninen, O., Laitinen, O. Long-term tissue response to bioabsorbable poly-L-lactide and metallic screws: an experimental study. Bone. 39 (4), 932-937 (2006).","journal-title":"Bone"},{"issue":"4","key":"ref30","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1002\/jbm.a.31142","type":"journal-article","article-title":"Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair","volume":"81","author":"Rai","year":"2007","unstructured":"Rai, B. et al. Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair. J. Biomed. Mater Res. A 81 (4), 888-899 (2007).","journal-title":"J. Biomed. Mater Res. A"},{"issue":"12","key":"ref31","doi-asserted-by":"crossref","first-page":"3449","DOI":"10.1089\/ten.2006.12.3449","type":"journal-article","article-title":"Kinetics of in vivo bone deposition by bone marrow stromal cells into porous calcium phosphate scaffolds: an X-ray computed microtomography study","volume":"12","author":"Komlev","year":"2006","unstructured":"Komlev, V. S. et al. Kinetics of in vivo bone deposition by bone marrow stromal cells into porous calcium phosphate scaffolds: an X-ray computed microtomography study. Tissue Eng. 12 (12), 3449-3458 (2006).","journal-title":"Tissue Eng"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/52940\/establishment-segmental-femoral-critical-size-defect-model-mice","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:12:07Z","timestamp":1792678327000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/52940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,12]]},"references-count":31,"journal-issue":{"issue":"116","published-online":{"date-parts":[[2016]]}},"URL":"https:\/\/doi.org\/10.3791\/52940","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,12]]}}}