{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:42:03Z","timestamp":1792683723342,"version":"4.5.0"},"reference-count":15,"publisher":"MyJove Corporation","issue":"110","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/53839","type":"journal-article","created":{"date-parts":[[2016,4,18]],"date-time":"2016-04-18T20:00:05Z","timestamp":1461009605000},"source":"Crossref","is-referenced-by-count":2,"title":["The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of <em>Coxiella burnetii<\/em>"],"prefix":"10.3791","author":[{"given":"Hua-Wei","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Mei","family":"Ching","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2016,4,18]]},"reference":[{"issue":"3","key":"ref1","first-page":"1","type":"journal-article","article-title":"Diagnosis and management of Q fever United States, 2013: recommendations from CDC and the Q Fever Working Group","volume":"62","author":"Anderson","year":"2013","unstructured":"Anderson, A., et al. Diagnosis and management of Q fever United States, 2013: recommendations from CDC and the Q Fever Working Group. MMWR Recomm. Rep. 62 (3), 1-30 (2013).","journal-title":"MMWR Recomm. Rep"},{"issue":"7","key":"ref2","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1128\/AAC.49.7.2673-2676.2005","type":"journal-article","article-title":"Correlation between ratio of serum doxycycline concentration to MIC and rapid decline of antibody levels during treatment of Q fever endocarditis","volume":"49","author":"Rolain","year":"2005","unstructured":"Rolain, J.M., Boulos,A., Mallet, M.N., Raoult, D. Correlation between ratio of serum doxycycline concentration to MIC and rapid decline of antibody levels during treatment of Q fever endocarditis. Antimicrob. Agents and Chemother. 49 (7), 2673-2676 (2005).","journal-title":"Antimicrob. Agents and Chemother"},{"issue":"11","key":"ref3","doi-asserted-by":"crossref","first-page":"5094","DOI":"10.1128\/JCM.41.11.5094-5098.2003","type":"journal-article","article-title":"Comparison of PCR and serology assays for early diagnosis of acute Q fever","volume":"41","author":"Fournier","year":"2003","unstructured":"Fournier, P.E., Raoult, D. Comparison of PCR and serology assays for early diagnosis of acute Q fever. J. Clin. Microbiol. 41 (11), 5094-5098 (2003).","journal-title":"J. Clin. Microbiol"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/CVI.00454-09","type":"journal-article","article-title":"Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever","volume":"17","author":"Schneeberger","year":"2010","unstructured":"Schneeberger, P.M., et al. Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever. Clin. Vaccine Immunol. 17 (2), 286-290 (2010).","journal-title":"Clin. Vaccine Immunol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1196\/annals.1374.112","type":"journal-article","article-title":"Diagnosis of acute Q fever by PCR on sera during a recent outbreak in rural South Australia","volume":"1078","author":"Turra","year":"2006","unstructured":"Turra, M., Chang, G., Whybrow, D., Higgins, G., Qiao, M. Diagnosis of acute Q fever by PCR on sera during a recent outbreak in rural South Australia. Ann. N Y Acad. Sci. 1078, 566-569 (2006).","journal-title":"Ann. N Y Acad. Sci"},{"issue":"12","key":"ref6","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/28.12.e63","type":"journal-article","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","unstructured":"Notomi, T., et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28 (12), E63 (2000).","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/j.trstmh.2008.04.040","type":"journal-article","article-title":"Simple, rapid and sensitive detection of Orientia tsutsugamushi. by loo-isothermal DNA amplification","volume":"102","author":"Paris","year":"2008","unstructured":"Paris, D.H., Blacksell, S.D., Newton, P.N., Day, N.P. Simple, rapid and sensitive detection of Orientia tsutsugamushi. by loo-isothermal DNA amplification. Trans. R. Soc. Trop. Med. Hyg. 102 (12), 1239-1246 (2008).","journal-title":"Trans. R. Soc. Trop. Med. Hyg"},{"key":"ref8","type":"book","article-title":"Loop-mediated isothermal amplification assay targeting the 47-kDa gene of Orientia tsutsugamushi.: A rapid and sensitive alternative to real-time PCR","volume-title":"J. Med. Microb. Diagn","author":"Huber","year":"2012","unstructured":"Huber, E., et al. Loop-mediated isothermal amplification assay targeting the 47-kDa gene of Orientia tsutsugamushi.: A rapid and sensitive alternative to real-time PCR. J. Med. Microb. Diagn. 1:112. (2012)."},{"issue":"254","key":"ref9","type":"journal-article","article-title":"Rapid, sensitive detection of the ompB gene of spotted fever group rickettsiae by loop-mediated isothermal amplification","volume":"12","author":"Pan","year":"2012","unstructured":"Pan, L., Zhang, L., Wang, G., Liu, Q. Rapid, simple, and sensitive detection of the ompB gene of spotted fever group rickettsiae by loop-mediated isothermal amplification. BMC Infect. Dis. 12:254. (2012).","journal-title":"BMC Infect. Dis"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/bbrc.2001.5921","type":"journal-article","article-title":"Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation","volume":"289","author":"Mori","year":"2001","unstructured":"Mori, Y., Nagamine, K., Tomita, N., Notomi, T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289. (1), 150-154 (2001).","journal-title":"Biochem. Biophys. Res. Commun"},{"issue":"12","key":"ref11","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1007\/s10529-007-9472-9","type":"journal-article","article-title":"Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis.spores","volume":"29","author":"Qiao","year":"2007","unstructured":"Qiao, Y.M., et al. Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis.spores. Biotechnol. Lett. 29. (12), 1939-1946 (2007).","journal-title":"Biotechnol. Lett"},{"issue":"5","key":"ref12","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1038\/nprot.2008.57","type":"journal-article","article-title":"Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products","volume":"3","author":"Tomita","year":"2008","unstructured":"Tomita, N., Mori, Y., Kanda, H., Notomi, T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Protoc. 3. (5), 877-882 (2008).","journal-title":"Nat. Protoc"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1292\/jvms.71.471","type":"journal-article","article-title":"Stability of Loop-Mediated Isothermal Amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates","volume":"71","author":"Thekisoe","year":"2009","unstructured":"Thekisoe, O.M., et al. Stability of Loop-Mediated Isothermal Amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates. J. Vet. Med. Sci. 71 (4), 471-475 (2009).","journal-title":"J. Vet. Med. Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.mimet.2014.07.039","type":"journal-article","article-title":"Development of loop-mediated isothermal amplification assays for rapid and easy detection of Coxiella Burnetii","volume":"107","author":"Chen","year":"2014","unstructured":"Chen, H.W., Ching, W.M. Development of loop-mediated isothermal amplification assays for rapid and easy detection of Coxiella Burnetii. J. Microbiol Methods. 107, 176-181 (2014).","journal-title":"J. Microbiol Methods"},{"issue":"6","key":"ref15","doi-asserted-by":"crossref","first-page":"9487","DOI":"10.3390\/molecules20069487","type":"journal-article","article-title":"A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria","volume":"20","author":"Wang","year":"2015","unstructured":"Wang, D., et al. A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria. Molecules. 20 (6), 9487-9495 (2015).","journal-title":"Molecules"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.jove.com\/video\/53839\/the-development-lyophilized-loop-mediated-isothermal-amplification","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:01:17Z","timestamp":1792681277000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/53839"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,18]]},"references-count":15,"journal-issue":{"issue":"110","published-online":{"date-parts":[[2016]]}},"URL":"https:\/\/doi.org\/10.3791\/53839","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,18]]}}}