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Structural mechanisms of DIAP1 auto-inhibition and DIAP1-mediated inhibition of drICE. Nat Commun. 2, 408 (2011).","journal-title":"Nat Commun"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1007\/s10495-006-0048-2","type":"journal-article","article-title":"Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis","volume":"12","author":"Yi","year":"2007","unstructured":"Yi, S. X., Moore, C. W., & Lee, R. E., Jr. Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis. Apoptosis. 12, 1183-1193 (2007).","journal-title":"Apoptosis"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1006\/dbio.2000.0135","type":"journal-article","article-title":"Stem cells and their progeny respond to nutritional changes during Drosophila oogenesis","volume":"231","author":"Drummond-Barbosa","year":"2001","unstructured":"Drummond-Barbosa, D., & Spradling, A. C. 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