{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:39:02Z","timestamp":1792676342438,"version":"4.5.0"},"reference-count":18,"publisher":"MyJove Corporation","issue":"126","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/55841","type":"journal-article","created":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T20:00:20Z","timestamp":1502136020000},"source":"Crossref","is-referenced-by-count":2,"title":["A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells"],"prefix":"10.3791","author":[{"given":"Dani\u00eblle G.","family":"Leuning","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Lievers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlies E.J.","family":"Reinders","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cees","family":"van Kooten","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marten A.","family":"Engelse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ton J.","family":"Rabelink","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2017,8,7]]},"reference":[{"issue":"2","key":"ref1","first-page":"83","type":"journal-article","article-title":"Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method","volume":"2","author":"Friedenstein","year":"1974","unstructured":"Friedenstein, A. J., et al. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol. 2 (2), 83-92 (1974).","journal-title":"Exp Hematol"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","type":"journal-article","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement","volume":"8","author":"Dominici","year":"2006","unstructured":"Dominici, M., et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 8 (4), 315-317 (2006).","journal-title":"Cytotherapy"},{"issue":"5411","key":"ref3","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1126\/science.284.5411.143","type":"journal-article","article-title":"Multilineage potential of adult human mesenchymal stem cells","volume":"284","author":"Pittenger","year":"1999","unstructured":"Pittenger, M. F., et al. Multilineage potential of adult human mesenchymal stem cells. Science. 284 (5411), 143-147 (1999).","journal-title":"Science"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.semnephrol.2014.06.002","type":"journal-article","article-title":"Clinical translation of multipotent mesenchymal stromal cells in transplantation","volume":"34","author":"Leuning","year":"2014","unstructured":"Leuning, D. G., Reinders, M. E., de Fijter, J. W., Rabelink, T. J. Clinical translation of multipotent mesenchymal stromal cells in transplantation. Semin Nephrol. 34 (4), 351-364, (2014).","journal-title":"Semin Nephrol"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"107","DOI":"10.5966\/sctm.2012-0114","type":"journal-article","article-title":"Autologous bone marrow-derived mesenchymal stromal cells for the treatment of allograft rejection after renal transplantation: results of a phase I study","volume":"2","author":"Reinders","year":"2013","unstructured":"Reinders, M. E., et al. Autologous bone marrow-derived mesenchymal stromal cells for the treatment of allograft rejection after renal transplantation: results of a phase I study. Stem Cells Transl Med. 2 (2), 107-111 (2013).","journal-title":"Stem Cells Transl Med"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"412","DOI":"10.2215\/CJN.04950610","type":"journal-article","article-title":"Autologous mesenchymal stromal cells and kidney transplantation: a pilot study of safety and clinical feasibility","volume":"6","author":"Perico","year":"2011","unstructured":"Perico, N., et al. Autologous mesenchymal stromal cells and kidney transplantation: a pilot study of safety and clinical feasibility. Clin J Am Soc Nephrol. 6 (2), 412-422 (2011).","journal-title":"Clin J Am Soc Nephrol"},{"issue":"9","key":"ref7","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/tri.12132","type":"journal-article","article-title":"Mesenchymal stromal cells and kidney transplantation: pretransplant infusion protects from graft dysfunction while fostering immunoregulation","volume":"26","author":"Perico","year":"2013","unstructured":"Perico, N., et al. Mesenchymal stromal cells and kidney transplantation: pretransplant infusion protects from graft dysfunction while fostering immunoregulation. Transpl Int. 26 (9), 867-878, (2013).","journal-title":"Transpl Int"},{"issue":"11","key":"ref8","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1001\/jama.2012.316","type":"journal-article","article-title":"Induction therapy with autologous mesenchymal stem cells in living-related kidney transplants: a randomized controlled trial","volume":"307","author":"Tan","year":"2012","unstructured":"Tan, J., et al. Induction therapy with autologous mesenchymal stem cells in living-related kidney transplants: a randomized controlled trial. JAMA. 307 (11), 1169-1177, (2012).","journal-title":"JAMA"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.stem.2008.07.003","type":"journal-article","article-title":"A perivascular origin for mesenchymal stem cells in multiple human organs","volume":"3","author":"Crisan","year":"2008","unstructured":"Crisan, M., et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 3 (3), 301-313 (2008).","journal-title":"Cell Stem Cell"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.stemcr.2016.05.011","type":"journal-article","article-title":"No Identical \"Mesenchymal Stem Cells\" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels","volume":"6","author":"Sacchetti","year":"2016","unstructured":"Sacchetti, B., et al. No Identical \"Mesenchymal Stem Cells\" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels. Stem Cell Reports. 6 (6), 897-913, (2016).","journal-title":"Stem Cell Reports"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/stem.1868","type":"journal-article","article-title":"Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity","volume":"33","author":"Chen","year":"2015","unstructured":"Chen, W. C., et al. Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity. Stem Cells. 33 (2), 557-573, (2015).","journal-title":"Stem Cells"},{"issue":"12","key":"ref12","doi-asserted-by":"crossref","first-page":"3300","DOI":"10.1681\/ASN.2005020195","type":"journal-article","article-title":"Isolation and characterization of nontubular sca-1+lin- multipotent stem\/progenitor cells from adult mouse kidney","volume":"17","author":"Dekel","year":"2006","unstructured":"Dekel, B., et al. Isolation and characterization of nontubular sca-1+lin- multipotent stem\/progenitor cells from adult mouse kidney. J Am Soc Nephrol. 17 (12), 3300-3314, (2006).","journal-title":"J Am Soc Nephrol"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1681\/ASN.2013050517","type":"journal-article","article-title":"Collecting duct-derived cells display mesenchymal stem cell properties and retain selective in vitro and in vivo epithelial capacity","volume":"26","author":"Li","year":"2015","unstructured":"Li, J., et al. Collecting duct-derived cells display mesenchymal stem cell properties and retain selective in vitro and in vivo epithelial capacity. J Am Soc Nephrol. 26 (1), 81-94, (2015).","journal-title":"J Am Soc Nephrol"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.scr.2011.08.003","type":"journal-article","article-title":"Comprehensive transcriptome and immunophenotype analysis of renal and cardiac MSC-like populations supports strong congruence with bone marrow MSC despite maintenance of distinct identities","volume":"8","author":"Pelekanos","year":"2012","unstructured":"Pelekanos, R. A., et al. Comprehensive transcriptome and immunophenotype analysis of renal and cardiac MSC-like populations supports strong congruence with bone marrow MSC despite maintenance of distinct identities. Stem Cell Res. 8 (1), 58-73, (2012).","journal-title":"Stem Cell Res"},{"key":"ref15","type":"journal-article","article-title":"Clinical-Grade Isolated Human Kidney Perivascular Stromal Cells as an Organotypic Cell Source for Kidney Regenerative Medicine","author":"Leuning","year":"2016","unstructured":"Leuning, D. G., et al. Clinical-Grade Isolated Human Kidney Perivascular Stromal Cells as an Organotypic Cell Source for Kidney Regenerative Medicine. Stem Cells Transl Med. (2016).","journal-title":"Stem Cells Transl Med"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1089\/scd.2008.0320","type":"journal-article","article-title":"Isolation and characterization of resident mesenchymal stem cells in human glomeruli","volume":"18","author":"Bruno","year":"2009","unstructured":"Bruno, S., et al. Isolation and characterization of resident mesenchymal stem cells in human glomeruli. Stem Cells Dev. 18 (6), 867-880, (2009).","journal-title":"Stem Cells Dev"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/ki.1994.6","type":"journal-article","article-title":"HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney","volume":"45","author":"Ryan","year":"1994","unstructured":"Ryan, M. J., et al. HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney. Kidney Int. 45 (1), 48-57 (1994).","journal-title":"Kidney Int"},{"key":"ref18","type":"journal-article","article-title":"Glycemic Stability Through Islet-After-Kidney Transplantation Using an Alemtuzumab-Based Induction Regimen and Long-Term Triple-Maintenance Immunosuppression","author":"Nijhoff","year":"2015","unstructured":"Nijhoff, M. F., et al. Glycemic Stability Through Islet-After-Kidney Transplantation Using an Alemtuzumab-Based Induction Regimen and Long-Term Triple-Maintenance Immunosuppression. Am J Transplant. (2015).","journal-title":"Am J Transplant"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/video\/55841\/a-novel-clinical-grade-isolation-method-for-human-kidney-perivascular","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:44:16Z","timestamp":1792673056000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/55841"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,7]]},"references-count":18,"journal-issue":{"issue":"126","published-online":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.3791\/55841","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,7]]}}}