{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:37:34Z","timestamp":1792676254847,"version":"4.5.0"},"reference-count":34,"publisher":"MyJove Corporation","issue":"126","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/56185","type":"journal-article","created":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T20:00:06Z","timestamp":1503345606000},"source":"Crossref","is-referenced-by-count":6,"title":["Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4"],"prefix":"10.3791","author":[{"given":"Sharon A.","family":"Sagan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Cruz-Herranz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Collin M.","family":"Spencer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy P.","family":"Ho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence","family":"Steinman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ari J.","family":"Green","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond A.","family":"Sobel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott S.","family":"Zamvil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2017,8,21]]},"reference":[{"issue":"9","key":"ref1","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/S1474-4422(16)30043-6","type":"journal-article","article-title":"Atypical inflammatory demyelinating syndromes of the CNS","volume":"15","author":"Hardy","year":"2016","unstructured":"Hardy, T. A. et al. Atypical inflammatory demyelinating syndromes of the CNS. Lancet Neurol. 15 (9), 967-981 (2016).","journal-title":"Lancet Neurol"},{"issue":"9451","key":"ref2","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1016\/S0140-6736(04)17551-X","type":"journal-article","article-title":"A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis","volume":"364","author":"Lennon","year":"2004","unstructured":"Lennon, V. A. et al. A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis. Lancet. 364 (9451), 2106-2112 (2004).","journal-title":"Lancet"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1084\/jem.20050304","type":"journal-article","article-title":"IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel","volume":"202","author":"Lennon","year":"2005","unstructured":"Lennon, V. A., Kryzer, T. J., Pittock, S. J., Verkman, A. S., & Hinson, S. R. IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J Exp Med. 202 (4), 473-477 (2005).","journal-title":"J Exp Med"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1212\/NXI.0000000000000062","type":"journal-article","article-title":"Does MOG Ig-positive AQP4-seronegative opticospinal inflammatory disease justify a diagnosis of NMO spectrum disorder","volume":"2","author":"Zamvil","year":"2015","unstructured":"Zamvil, S. S., & Slavin, A. J. Does MOG Ig-positive AQP4-seronegative opticospinal inflammatory disease justify a diagnosis of NMO spectrum disorder? Neurol Neuroimmunol Neuroinflamm. 2 (1), e62 (2015).","journal-title":"Neurol Neuroimmunol Neuroinflamm"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1002\/ana.21802","type":"journal-article","article-title":"Intrathecal pathogenic anti-aquaporin-4 antibodies in early neuromyelitis optica","volume":"66","author":"Bennett","year":"2009","unstructured":"Bennett, J. L. et al. Intrathecal pathogenic anti-aquaporin-4 antibodies in early neuromyelitis optica. Ann Neurol. 66 (5), 617-629 (2009).","journal-title":"Ann Neurol"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1002\/ana.21837","type":"journal-article","article-title":"Neuromyelitis optica: pathogenicity of patient immunoglobulin in vivo","volume":"66","author":"Bradl","year":"2009","unstructured":"Bradl, M. et al. Neuromyelitis optica: pathogenicity of patient immunoglobulin in vivo. Ann Neurol. 66 (5), 630-643 (2009).","journal-title":"Ann Neurol"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/cmi.2010.24","type":"journal-article","article-title":"Understanding the development and function of T follicular helper cells","volume":"7","author":"Nurieva","year":"2010","unstructured":"Nurieva, R. I., & Chung, Y. Understanding the development and function of T follicular helper cells. Cell Mol Immunol. 7 (3), 190-197 (2010).","journal-title":"Cell Mol Immunol"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/bpa.12099","type":"journal-article","article-title":"The pathology of an autoimmune astrocytopathy: lessons learned from neuromyelitis optica","volume":"24","author":"Lucchinetti","year":"2014","unstructured":"Lucchinetti, C. F. et al. The pathology of an autoimmune astrocytopathy: lessons learned from neuromyelitis optica. Brain Pathol. 24 (1), 83-97 (2014).","journal-title":"Brain Pathol"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1212\/NXI.0000000000000110","type":"journal-article","article-title":"Aquaporin-4 autoimmunity","volume":"2","author":"Zekeridou","year":"2015","unstructured":"Zekeridou, A., & Lennon, V. A. Aquaporin-4 autoimmunity. Neurol Neuroimmunol Neuroinflamm. 2 (4), e110 (2015).","journal-title":"Neurol Neuroimmunol Neuroinflamm"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1177\/1352458509350741","type":"journal-article","article-title":"HLA-DRB association in neuromyelitis optica is different from that observed in multiple sclerosis","volume":"16","author":"Brum","year":"2010","unstructured":"Brum, D. G. et al. HLA-DRB association in neuromyelitis optica is different from that observed in multiple sclerosis. Mult Scler. 16 (1), 21-29 (2010).","journal-title":"Mult Scler"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1002\/ana.23651","type":"journal-article","article-title":"Aquaporin 4-specific T cells in neuromyelitis optica exhibit a Th17 bias and recognize Clostridium ABC transporter","volume":"72","author":"Varrin-Doyer","year":"2012","unstructured":"Varrin-Doyer, M. et al. Aquaporin 4-specific T cells in neuromyelitis optica exhibit a Th17 bias and recognize Clostridium ABC transporter. Ann Neurol. 72 (1), 53-64 (2012).","journal-title":"Ann Neurol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.msard.2015.12.004","type":"journal-article","article-title":"T-cell responses to distinct AQP4 peptides in patients with neuromyelitis optica (NMO)","volume":"6","author":"Vaknin-Dembinsky","year":"2016","unstructured":"Vaknin-Dembinsky, A. et al. T-cell responses to distinct AQP4 peptides in patients with neuromyelitis optica (NMO). Mult Scler Relat Disord. 6 28-36 (2016).","journal-title":"Mult Scler Relat Disord"},{"issue":"11","key":"ref13","doi-asserted-by":"crossref","first-page":"15050","DOI":"10.1371\/journal.pone.0015050","type":"journal-article","article-title":"Immunodominant T cell determinants of aquaporin-4, the autoantigen associated with neuromyelitis optica","volume":"5","author":"Nelson","year":"2010","unstructured":"Nelson, P. A. et al. Immunodominant T cell determinants of aquaporin-4, the autoantigen associated with neuromyelitis optica. PLoS One. 5 (11), e15050 (2010).","journal-title":"PLoS One"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"16083","DOI":"10.1371\/journal.pone.0016083","type":"journal-article","article-title":"Functional characterization of aquaporin-4 specific T cells: towards a model for neuromyelitis optica","volume":"6","author":"Kalluri","year":"2011","unstructured":"Kalluri, S. R. et al. Functional characterization of aquaporin-4 specific T cells: towards a model for neuromyelitis optica. PLoS One. 6 (1), e16083 (2011).","journal-title":"PLoS One"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00401-011-0824-0","type":"journal-article","article-title":"Pathogenic T cell responses against aquaporin 4","volume":"122","author":"Pohl","year":"2011","unstructured":"Pohl, M. et al. Pathogenic T cell responses against aquaporin 4. Acta Neuropathol. 122 (1), 21-34 (2011).","journal-title":"Acta Neuropathol"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1007\/s00401-015-1501-5","type":"journal-article","article-title":"Highly encephalitogenic aquaporin 4-specific T cells and NMO-IgG jointly orchestrate lesion location and tissue damage in the CNS","volume":"130","author":"Zeka","year":"2015","unstructured":"Zeka, B. et al. Highly encephalitogenic aquaporin 4-specific T cells and NMO-IgG jointly orchestrate lesion location and tissue damage in the CNS. Acta Neuropathol. 130 (6), 783-798 (2015).","journal-title":"Acta Neuropathol"},{"issue":"51","key":"ref17","doi-asserted-by":"crossref","first-page":"14781","DOI":"10.1073\/pnas.1617859114","type":"journal-article","article-title":"Tolerance checkpoint bypass permits emergence of pathogenic T cells to neuromyelitis optica autoantigen aquaporin-4","volume":"113","author":"Sagan","year":"2016","unstructured":"Sagan, S. A. et al. Tolerance checkpoint bypass permits emergence of pathogenic T cells to neuromyelitis optica autoantigen aquaporin-4. Proc Natl Acad Sci U S A. 113 (51), 14781-14786 (2016).","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1093\/nar\/gku938","type":"journal-article","article-title":"The immune epitope database (IEDB) 3.0","volume":"43","author":"Vita","year":"2015","unstructured":"Vita, R. et al. The immune epitope database (IEDB) 3.0. Nucleic Acids Res. 43 (Database issue), D405-412 (2015).","journal-title":"Nucleic Acids Res"},{"issue":"1-2","key":"ref19","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.jim.2007.11.012","type":"journal-article","article-title":"Lymph node mapping in the mouse","volume":"332","author":"Harrell","year":"2008","unstructured":"Harrell, M. I., Iritani, B. M., & Ruddell, A. Lymph node mapping in the mouse. J Immunol Methods. 332 (1-2), 170-174 (2008).","journal-title":"J Immunol Methods"},{"issue":"82","key":"ref20","first-page":"e51018","type":"journal-article","article-title":"Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry","author":"Ivanova","year":"2013","unstructured":"Ivanova, E., Toychiev, A. H., Yee, C. W., & Sagdullaev, B. T. Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry. J Vis Exp. (82), e51018 (2013).","journal-title":"J Vis Exp"},{"issue":"24","key":"ref21","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1212\/WNL.0000000000002774","type":"journal-article","article-title":"The APOSTEL recommendations for reporting quantitative optical coherence tomography studies","volume":"86","author":"Cruz-Herranz","year":"2016","unstructured":"Cruz-Herranz, A. et al. The APOSTEL recommendations for reporting quantitative optical coherence tomography studies. Neurology. 86 (24), 2303-2309 (2016).","journal-title":"Neurology"},{"issue":"7","key":"ref22","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1002\/eji.1830250723","type":"journal-article","article-title":"A myelin oligodendrocyte glycoprotein peptide induces typical chronic experimental autoimmune encephalomyelitis in H-2b mice: fine specificity and T cell receptor V beta expression of encephalitogenic T cells","volume":"25","author":"Mendel","year":"1995","unstructured":"Mendel, I., Kerlero de Rosbo, N., & Ben-Nun, A. A myelin oligodendrocyte glycoprotein peptide induces typical chronic experimental autoimmune encephalomyelitis in H-2b mice: fine specificity and T cell receptor V beta expression of encephalitogenic T cells. Eur J Immunol. 25 (7), 1951-1959 (1995).","journal-title":"Eur J Immunol"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1212\/NXI.0000000000000022","type":"journal-article","article-title":"Immunodominant T-cell epitopes of MOG reside in its transmembrane and cytoplasmic domains in EAE","volume":"1","author":"Shetty","year":"2014","unstructured":"Shetty, A. et al. Immunodominant T-cell epitopes of MOG reside in its transmembrane and cytoplasmic domains in EAE. Neurol Neuroimmunol Neuroinflamm. 1 (2), e22 (2014).","journal-title":"Neurol Neuroimmunol Neuroinflamm"},{"issue":"13","key":"ref24","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1084\/jem.20130699","type":"journal-article","article-title":"MHC class II-dependent B cell APC function is required for induction of CNS autoimmunity independent of myelin-specific antibodies","volume":"210","author":"Molnarfi","year":"2013","unstructured":"Molnarfi, N. et al. MHC class II-dependent B cell APC function is required for induction of CNS autoimmunity independent of myelin-specific antibodies. J Exp Med. 210 (13), 2921-2937 (2013).","journal-title":"J Exp Med"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40478-015-0207-1","type":"journal-article","article-title":"Pathogenic aquaporin-4 reactive T cells are sufficient to induce mouse model of neuromyelitis optica","volume":"3","author":"Jones","year":"2015","unstructured":"Jones, M. V., Huang, H., Calabresi, P. A., & Levy, M. Pathogenic aquaporin-4 reactive T cells are sufficient to induce mouse model of neuromyelitis optica. Acta Neuropathol Commun. 3 28 (2015).","journal-title":"Acta Neuropathol Commun"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1212\/NXI.0000000000000334","type":"journal-article","article-title":"Microstructural visual system changes in AQP4-antiboby-seropositive NMOSD","volume":"4","author":"Oertel","year":"2017","unstructured":"Oertel, F. C. et al. Microstructural visual system changes in AQP4-antiboby-seropositive NMOSD. Neurol. Neuroimmunol. Neuroinflamm. 4 e72 (2017).","journal-title":"Neurol. Neuroimmunol. Neuroinflamm"},{"key":"ref27","doi-asserted-by":"crossref","DOI":"10.1002\/eji.201646855","type":"journal-article","article-title":"Deletional tolerance prevents AQP4 directed autoimmunity in mice","author":"Vogel","year":"2017","unstructured":"Vogel, A. L. et al. Deletional tolerance prevents AQP4 directed autoimmunity in mice. Eur J Immunol. (2017).","journal-title":"Eur J Immunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jaut.2014.02.011","type":"journal-article","article-title":"Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59","volume":"53","author":"Zhang","year":"2014","unstructured":"Zhang, H., & Verkman, A. S. Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59. J Autoimmun. 53 67-77 (2014).","journal-title":"J Autoimmun"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s40478-016-0355-y","type":"journal-article","article-title":"Aquaporin 4-specific T cells and NMO-IgG cause primary retinal damage in experimental NMO\/SD","volume":"4","author":"Zeka","year":"2016","unstructured":"Zeka, B. et al. Aquaporin 4-specific T cells and NMO-IgG cause primary retinal damage in experimental NMO\/SD. Acta Neuropathol Commun. 4 (1), 82 (2016).","journal-title":"Acta Neuropathol Commun"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1186\/s12974-016-0746-9","type":"journal-article","article-title":"Complement-independent retinal pathology produced by intravitreal injection of neuromyelitis optica immunoglobulin G","volume":"13","author":"Felix","year":"2016","unstructured":"Felix, C. M., Levin, M. H., & Verkman, A. S. Complement-independent retinal pathology produced by intravitreal injection of neuromyelitis optica immunoglobulin G. J Neuroinflammation. 13 (1), 275 (2016).","journal-title":"J Neuroinflammation"},{"issue":"6035","key":"ref31","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/317355a0","type":"journal-article","article-title":"T-cell clones specific for myelin basic protein induce chronic relapsing paralysis and demyelination","volume":"317","author":"Zamvil","year":"1985","unstructured":"Zamvil, S. et al. T-cell clones specific for myelin basic protein induce chronic relapsing paralysis and demyelination. Nature. 317 (6035), 355-358 (1985).","journal-title":"Nature"},{"issue":"6","key":"ref32","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1084\/jem.162.6.2107","type":"journal-article","article-title":"Encephalitogenic T cell clones specific for myelin basic protein. An unusual bias in antigen recognition","volume":"162","author":"Zamvil","year":"1985","unstructured":"Zamvil, S. S. et al. Encephalitogenic T cell clones specific for myelin basic protein. An unusual bias in antigen recognition. J Exp Med. 162 (6), 2107-2124 (1985).","journal-title":"J Exp Med"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/0092-8674(95)90349-6","type":"journal-article","article-title":"B7-1 and B7-2 costimulatory molecules activate differentially the Th1\/Th2 developmental pathways: application to autoimmune disease therapy","volume":"80","author":"Kuchroo","year":"1995","unstructured":"Kuchroo, V. K. et al. B7-1 and B7-2 costimulatory molecules activate differentially the Th1\/Th2 developmental pathways: application to autoimmune disease therapy. Cell. 80 (5), 707-718 (1995).","journal-title":"Cell"},{"issue":"7090","key":"ref34","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/nature04753","type":"journal-article","article-title":"Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells","volume":"441","author":"Bettelli","year":"2006","unstructured":"Bettelli, E. et al. Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells. Nature. 441 (7090), 235-238 (2006).","journal-title":"Nature"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/video\/56185\/induction-paralysis-visual-system-injury-mice-t-cells-specific-for","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:37:35Z","timestamp":1792672655000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/56185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,21]]},"references-count":34,"journal-issue":{"issue":"126","published-online":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.3791\/56185","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,21]]}}}