{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:45:40Z","timestamp":1792669540790,"version":"4.5.0"},"reference-count":19,"publisher":"MyJove Corporation","issue":"133","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/57155","type":"journal-article","created":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T19:00:09Z","timestamp":1520622009000},"source":"Crossref","is-referenced-by-count":8,"title":["Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis"],"prefix":"10.3791","author":[{"given":"Dylan J.","family":"Stolz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethan M.","family":"Sands","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nansalmaa","family":"Amarsaikhan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angar","family":"Tsoggerel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven P.","family":"Templeton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2018,3,9]]},"reference":[{"issue":"11","key":"ref1","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1128\/EC.00274-07","type":"journal-article","article-title":"Aspergillus fumigatus: principles of pathogenesis and host defense","volume":"6","author":"Hohl","year":"2007","unstructured":"Hohl, T. M., & Feldmesser, M. Aspergillus fumigatus: principles of pathogenesis and host defense. Eukaryot Cell. 6 (11), 1953-1963 (2007).","journal-title":"Eukaryot Cell"},{"issue":"12","key":"ref2","doi-asserted-by":"crossref","first-page":"e1003743","DOI":"10.1371\/journal.ppat.1003743","type":"journal-article","article-title":"Aspergillus fumigatus--what makes the species a ubiquitous human fungal pathogen?","volume":"9","author":"Kwon-Chung","year":"2013","unstructured":"Kwon-Chung, K. J., & Sugui, J. A. Aspergillus fumigatus--what makes the species a ubiquitous human fungal pathogen? PLoS Pathog. 9 (12), e1003743 (2013).","journal-title":"PLoS Pathog"},{"issue":"9","key":"ref3","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1038\/nrd3074","type":"journal-article","article-title":"An insight into the antifungal pipeline: selected new molecules and beyond","volume":"9","author":"Ostrosky-Zeichner","year":"2010","unstructured":"Ostrosky-Zeichner, L., Casadevall, A., Galgiani, J. N., Odds, F. C., & Rex, J. H. An insight into the antifungal pipeline: selected new molecules and beyond. Nat Rev Drug Discov. 9 (9), 719-727 (2010).","journal-title":"Nat Rev Drug Discov"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"841","DOI":"10.3389\/fmicb.2017.00841","type":"journal-article","article-title":"Rodent Models of Invasive Aspergillosis due to Aspergillus fumigatus: Still a Long Path toward Standardization","volume":"8","author":"Desoubeaux","year":"2017","unstructured":"Desoubeaux, G., & Cray, C. Rodent Models of Invasive Aspergillosis due to Aspergillus fumigatus: Still a Long Path toward Standardization. Front Microbiol. 8 841 (2017).","journal-title":"Front Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jim.2014.03.022","type":"journal-article","article-title":"Overview of vertebrate animal models of fungal infection","volume":"410","author":"Hohl","year":"2014","unstructured":"Hohl, T. M. Overview of vertebrate animal models of fungal infection. J Immunol Methods. 410 100-112 (2014).","journal-title":"J Immunol Methods"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1469-0691.2005.01349.x","type":"journal-article","article-title":"Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis","volume":"12","author":"Sheppard","year":"2006","unstructured":"Sheppard, D. C. et al. Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis. Clin Microbiol Infect. 12 (4), 376-380 (2006).","journal-title":"Clin Microbiol Infect"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"S115","DOI":"10.1080\/13693780400029429","type":"journal-article","article-title":"The future of animal models of invasive aspergillosis","volume":"43 Suppl 1","author":"Patterson","year":"2005","unstructured":"Patterson, T. F. The future of animal models of invasive aspergillosis. Med Mycol. 43 Suppl 1 S115-119 (2005).","journal-title":"Med Mycol"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"624","DOI":"10.4049\/jimmunol.1700078","type":"journal-article","article-title":"Caspofungin Increases Fungal Chitin and Eosinophil and gammadelta T Cell-Dependent Pathology in Invasive Aspergillosis","volume":"199","author":"Amarsaikhan","year":"2017","unstructured":"Amarsaikhan, N. et al. Caspofungin Increases Fungal Chitin and Eosinophil and gammadelta T Cell-Dependent Pathology in Invasive Aspergillosis. J Immunol. 199 (2), 624-632 (2017).","journal-title":"J Immunol"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"e18777","DOI":"10.1371\/journal.pone.0018777","type":"journal-article","article-title":"Role of germination in murine airway CD8+ T-cell responses to Aspergillus conidia","volume":"6","author":"Templeton","year":"2011","unstructured":"Templeton, S. P., Buskirk, A. D., Law, B., Green, B. J., & Beezhold, D. H. Role of germination in murine airway CD8+ T-cell responses to Aspergillus conidia. PLoS One. 6 (4), e18777 (2011).","journal-title":"PLoS One"},{"issue":"122","key":"ref10","doi-asserted-by":"crossref","DOI":"10.3791\/55444","type":"journal-article","article-title":"Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus","author":"Brunel","year":"2017","unstructured":"Brunel, S. F. et al. Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus. J Vis Exp. (122) (2017).","journal-title":"J Vis Exp"},{"issue":"15","key":"ref11","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1080\/15287390306417","type":"journal-article","article-title":"Efficacy of a technique for exposing the mouse lung to particles aspirated from the pharynx","volume":"66","author":"Rao","year":"2003","unstructured":"Rao, G. V. et al. Efficacy of a technique for exposing the mouse lung to particles aspirated from the pharynx. J Toxicol Environ Health A. 66 (15), 1441-1452 (2003).","journal-title":"J Toxicol Environ Health A"},{"issue":"12","key":"ref12","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1128\/AAC.45.12.3474-3481.2001","type":"journal-article","article-title":"Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis: demonstration of efficacy of caspofungin acetate","volume":"45","author":"Bowman","year":"2001","unstructured":"Bowman, J. C. et al. Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis: demonstration of efficacy of caspofungin acetate. Antimicrob Agents Chemother. 45 (12), 3474-3481 (2001).","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1128\/EC.00288-10","type":"journal-article","article-title":"The small GTPase RacA mediates intracellular reactive oxygen species production, polarized growth, and virulence in the human fungal pathogen Aspergillus fumigatus","volume":"10","author":"Li","year":"2011","unstructured":"Li, H. et al. The small GTPase RacA mediates intracellular reactive oxygen species production, polarized growth, and virulence in the human fungal pathogen Aspergillus fumigatus. Eukaryot Cell. 10 (2), 174-186 (2011).","journal-title":"Eukaryot Cell"},{"issue":"10","key":"ref14","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1345\/aph.1M237","type":"journal-article","article-title":"Echinocandins: the newest class of antifungals","volume":"43","author":"Sucher","year":"2009","unstructured":"Sucher, A. J., Chahine, E. B., & Balcer, H. E. Echinocandins: the newest class of antifungals. Ann Pharmacother. 43 (10), 1647-1657 (2009).","journal-title":"Ann Pharmacother"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1128\/JCM.02073-08","type":"journal-article","article-title":"Strain-dependent variation in 18S ribosomal DNA Copy numbers in Aspergillus fumigatus","volume":"47","author":"Herrera","year":"2009","unstructured":"Herrera, M. L., Vallor, A. C., Gelfond, J. A., Patterson, T. F., & Wickes, B. L. Strain-dependent variation in 18S ribosomal DNA Copy numbers in Aspergillus fumigatus. J Clin Microbiol. 47 (5), 1325-1332 (2009).","journal-title":"J Clin Microbiol"},{"issue":"8","key":"ref16","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.1128\/AAC.46.8.2564-2568.2002","type":"journal-article","article-title":"Efficacy of caspofungin alone and in combination with voriconazole in a Guinea pig model of invasive aspergillosis","volume":"46","author":"Kirkpatrick","year":"2002","unstructured":"Kirkpatrick, W. R., Perea, S., Coco, B. J., & Patterson, T. F. Efficacy of caspofungin alone and in combination with voriconazole in a Guinea pig model of invasive aspergillosis. Antimicrob Agents Chemother. 46 (8), 2564-2568 (2002).","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1128\/AAC.46.1.12-23.2002","type":"journal-article","article-title":"Antifungal efficacy of caspofungin (MK-0991) in experimental pulmonary aspergillosis in persistently neutropenic rabbits: pharmacokinetics, drug disposition, and relationship to galactomannan antigenemia","volume":"46","author":"Petraitiene","year":"2002","unstructured":"Petraitiene, R. et al. Antifungal efficacy of caspofungin (MK-0991) in experimental pulmonary aspergillosis in persistently neutropenic rabbits: pharmacokinetics, drug disposition, and relationship to galactomannan antigenemia. Antimicrob Agents Chemother. 46 (1), 12-23 (2002).","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1002\/ppul.23618","type":"journal-article","article-title":"Aspergillosis and the role of mucins in cystic fibrosis","volume":"52","author":"Cowley","year":"2017","unstructured":"Cowley, A. C., Thornton, D. J., Denning, D. W., & Horsley, A. Aspergillosis and the role of mucins in cystic fibrosis. Pediatr Pulmonol. 52 (4), 548-555 (2017).","journal-title":"Pediatr Pulmonol"},{"issue":"5","key":"ref19","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1067\/mhn.2002.128896","type":"journal-article","article-title":"Detection of fungal organisms in eosinophilic mucin using a fluorescein-labeled chitin-specific binding protein","volume":"127","author":"Taylor","year":"2002","unstructured":"Taylor, M. J. et al. Detection of fungal organisms in eosinophilic mucin using a fluorescein-labeled chitin-specific binding protein. Otolaryngol Head Neck Surg. 127 (5), 377-383 (2002).","journal-title":"Otolaryngol Head Neck Surg"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/video\/57155\/histological-quantification-to-determine-lung-fungal-burden","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:36:20Z","timestamp":1792668980000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/57155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,9]]},"references-count":19,"journal-issue":{"issue":"133","published-online":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.3791\/57155","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,9]]}}}