{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:42:25Z","timestamp":1792669345481,"version":"4.5.0"},"reference-count":24,"publisher":"MyJove Corporation","issue":"135","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/57241","type":"journal-article","created":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T20:00:07Z","timestamp":1526932807000},"source":"Crossref","is-referenced-by-count":5,"title":["High-throughput Identification of Synergistic Drug Combinations by the Overlap&lt;sup&gt;2&lt;\/sup&gt; Method"],"prefix":"10.3791","author":[{"given":"Morgan A.","family":"Wambaugh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica C. S.","family":"Brown","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2018,5,21]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3389\/fpubh.2014.00145","type":"journal-article","article-title":"The Antimicrobial Resistance Crisis: Causes, Consequences, and Management","volume":"2","author":"Michael","year":"2014","unstructured":"Michael, C. A., Dominey-Howes, D., & Labbate, M. The Antimicrobial Resistance Crisis: Causes, Consequences, and Management. Front Public Health. 2 145 (2014).","journal-title":"Front Public Health"},{"issue":"9","key":"ref2","doi-asserted-by":"crossref","first-page":"e1002870","DOI":"10.1371\/journal.ppat.1002870","type":"journal-article","article-title":"Antifungal Drug Discovery: Something Old and Something New","volume":"8","author":"Butts","year":"2012","unstructured":"Butts, A., & Krysan, D. J. Antifungal Drug Discovery: Something Old and Something New. PLOS Pathogens. 8 (9), e1002870 (2012).","journal-title":"PLOS Pathogens"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"a019703","DOI":"10.1101\/cshperspect.a019703","type":"journal-article","article-title":"Antifungal Drug Development: Challenges, Unmet Clinical Needs, and New Approaches","volume":"4","author":"Roemer","year":"2014","unstructured":"Roemer, T., & Krysan, D. J. Antifungal Drug Development: Challenges, Unmet Clinical Needs, and New Approaches. Cold Spring Harb Perspect Med. 4 (5), a019703 (2014).","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1208\/s12248-012-9390-1","type":"journal-article","article-title":"Drug Repurposing: Far Beyond New Targets for Old Drugs","volume":"14","author":"Oprea","year":"2012","unstructured":"Oprea, T. I., & Mestres, J. Drug Repurposing: Far Beyond New Targets for Old Drugs. AAPS J. 14 (4), 759-763 (2012).","journal-title":"AAPS J"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/nrd3681","type":"journal-article","article-title":"Diagnosing the decline in pharmaceutical R&D efficiency","volume":"11","author":"Scannell","year":"2012","unstructured":"Scannell, J. W., Blanckley, A., Boldon, H., & Warrington, B. Diagnosing the decline in pharmaceutical R&D efficiency. Nat Rev Drug Discov. 11 (3), 191-200 (2012).","journal-title":"Nat Rev Drug Discov"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"282","DOI":"10.3389\/fmicb.2015.00282","type":"journal-article","article-title":"Drug repurposing as an alternative for the treatment of recalcitrant bacterial infections","volume":"6","author":"Rangel-Vega","year":"2015","unstructured":"Rangel-Vega, A., Bernstein, L. R., Mandujano-Tinoco, E. A., Garc\u00eda-Contreras, S. J., & Garc\u00eda-Contreras, R. Drug repurposing as an alternative for the treatment of recalcitrant bacterial infections. Front Microbiol. 6 282 (2015).","journal-title":"Front Microbiol"},{"issue":"6","key":"ref7","doi-asserted-by":"crossref","first-page":"e1000796","DOI":"10.1371\/journal.pcbi.1000796","type":"journal-article","article-title":"Optimal Drug Synergy in Antimicrobial Treatments","volume":"6","author":"Torella","year":"2010","unstructured":"Torella, J. P., Chait, R., & Kishony, R. Optimal Drug Synergy in Antimicrobial Treatments. PLoS Comput Biol. 6 (6), e1000796 (2010).","journal-title":"PLoS Comput Biol"},{"issue":"8","key":"ref8","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1073\/pnas.0813394106","type":"journal-article","article-title":"Harnessing Hsp90 function as a powerful, broadly effective therapeutic strategy for fungal infectious disease","volume":"106","author":"Cowen","year":"2009","unstructured":"Cowen, L. E. et al. Harnessing Hsp90 function as a powerful, broadly effective therapeutic strategy for fungal infectious disease. P Natl Acad Sci. 106 (8), 2818-2823 (2009).","journal-title":"P Natl Acad Sci"},{"issue":"12","key":"ref9","doi-asserted-by":"crossref","first-page":"9819","DOI":"10.3390\/molecules16129819","type":"journal-article","article-title":"Synergistic Antibacterial and Antibiotic Effects of Bisbenzylisoquinoline Alkaloids on Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA)","volume":"16","author":"Zuo","year":"2011","unstructured":"Zuo, G.-Y. et al. Synergistic Antibacterial and Antibiotic Effects of Bisbenzylisoquinoline Alkaloids on Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA). Molecules. 16 (12), 9819 (2011).","journal-title":"Molecules"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nrd2683","type":"journal-article","article-title":"Mechanisms of drug combinations: interaction and network perspectives","volume":"8","author":"Jia","year":"2009","unstructured":"Jia, J. et al. Mechanisms of drug combinations: interaction and network perspectives. Nat Rev Drug Discov. 8 (2), 111-128 (2009).","journal-title":"Nat Rev Drug Discov"},{"issue":"6","key":"ref11","doi-asserted-by":"crossref","first-page":"e2001644","DOI":"10.1371\/journal.pbio.2001644","type":"journal-article","article-title":"High-throughput identification and rational design of synergistic small-molecule pairs for combating and bypassing antibiotic resistance","volume":"15","author":"Wambaugh","year":"2017","unstructured":"Wambaugh, M. A., Shakya, V. P. S., Lewis, A. J., Mulvey, M. A., & Brown, J. C. S. High-throughput identification and rational design of synergistic small-molecule pairs for combating and bypassing antibiotic resistance. PLOS Biol. 15 (6), e2001644 (2017).","journal-title":"PLOS Biol"},{"issue":"5","key":"ref12","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.cell.2014.10.044","type":"journal-article","article-title":"Unraveling the biology of a fungal meningitis pathogen using chemical genetics","volume":"159","author":"Brown","year":"2014","unstructured":"Brown, J. C. S. et al. Unraveling the biology of a fungal meningitis pathogen using chemical genetics. Cell. 159 (5), 1168-1187 (2014).","journal-title":"Cell"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1038\/msb.2011.71","type":"journal-article","article-title":"Systematic exploration of synergistic drug pairs","volume":"7","author":"Cokol","year":"2011","unstructured":"Cokol, M. et al. Systematic exploration of synergistic drug pairs. Mol Syst Biol. 7 544-544 (2011).","journal-title":"Mol Syst Biol"},{"issue":"13","key":"ref14","doi-asserted-by":"crossref","first-page":"7977","DOI":"10.1073\/pnas.1337088100","type":"journal-article","article-title":"Systematic discovery of multicomponent therapeutics","volume":"100","author":"Borisy","year":"2003","unstructured":"Borisy, A. A. et al. Systematic discovery of multicomponent therapeutics. Proc Natl Acad Sci USA. 100 (13), 7977-7982 (2003).","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3389\/fphar.2015.00181","type":"journal-article","article-title":"What is synergy? The Saariselk\u00e4 agreement revisited","volume":"6","author":"Tang","year":"2015","unstructured":"Tang, J., Wennerberg, K., & Aittokallio, T. What is synergy? The Saariselk\u00e4 agreement revisited. Front Pharmacol. 6 181 (2015).","journal-title":"Front Pharmacol"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/0732-8893(93)90087-N","type":"journal-article","article-title":"Synergy assessed by checkerboard. A critical analysis","volume":"16","author":"Hsieh","year":"1993","unstructured":"Hsieh, M. H., Yu, C. M., Yu, V. L., & Chow, J. W. Synergy assessed by checkerboard. A critical analysis. Diagn Microbiol Infect Dis. 16 (4), 343-349 (1993).","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.cell.2010.11.052","type":"journal-article","article-title":"Phenotypic Landscape of a Bacterial Cell","volume":"144","author":"Nichols","year":"2011","unstructured":"Nichols, R. J. et al. Phenotypic Landscape of a Bacterial Cell. Cell. 144 (1), 143-156 (2011).","journal-title":"Cell"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","DOI":"10.15252\/msb.20156777","type":"journal-article","article-title":"Chemogenomics and orthology-based design of antibiotic combination therapies","volume":"12","author":"Chandrasekaran","year":"2016","unstructured":"Chandrasekaran, S. et al. Chemogenomics and orthology-based design of antibiotic combination therapies. Mol Syst Biol. 12 (5), 872 (2016).","journal-title":"Mol Syst Biol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"15930","DOI":"10.1038\/srep15930","type":"journal-article","article-title":"Chemogenomic profiling of Plasmodium falciparum as a tool to aid antimalarial drug discovery","volume":"5","author":"Pradhan","year":"2015","unstructured":"Pradhan, A. et al. Chemogenomic profiling of Plasmodium falciparum as a tool to aid antimalarial drug discovery. Sci Rep. 5 15930 (2015).","journal-title":"Sci Rep"},{"issue":"5","key":"ref20","first-page":"1","type":"journal-article","article-title":"A chemical genetic screen in Mycobacterium tuberculosis identifies carbon-source-dependent growth inhibitors devoid of in vivo efficacy","volume":"1","author":"Pethe","year":"2010","unstructured":"Pethe, K. et al. A chemical genetic screen in Mycobacterium tuberculosis identifies carbon-source-dependent growth inhibitors devoid of in vivo efficacy. Nat Commun. 1 (5), 1-8 (2010).","journal-title":"Nat Commun"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"e1002562","DOI":"10.1371\/journal.pgen.1002562","type":"journal-article","article-title":"Mapping the Hsp90 Genetic Interaction Network in Candida albicans Reveals Environmental Contingency and Rewired Circuitry","volume":"8","author":"Diezmann","year":"2012","unstructured":"Diezmann, S., Michaut, M., Shapiro, R. S., Bader, G. D., & Cowen, L. E. Mapping the Hsp90 Genetic Interaction Network in Candida albicans Reveals Environmental Contingency and Rewired Circuitry. PLoS Genetics. 8 (3), e1002562 (2012).","journal-title":"PLoS Genetics"},{"issue":"7","key":"ref22","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1016\/j.celrep.2015.10.018","type":"journal-article","article-title":"An Antifungal Combination Matrix Identifies a Rich Pool of Adjuvant Molecules that Enhance Drug Activity Against Diverse Fungal Pathogens","volume":"13","author":"Robbins","year":"2015","unstructured":"Robbins, N. et al. An Antifungal Combination Matrix Identifies a Rich Pool of Adjuvant Molecules that Enhance Drug Activity Against Diverse Fungal Pathogens. Cell reports. 13 (7), 1481-1492 (2015).","journal-title":"Cell reports"},{"issue":"6","key":"ref23","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.cels.2015.12.003","type":"journal-article","article-title":"Prediction of Synergism from Chemical-Genetic Interactions by Machine Learning","volume":"1","author":"Wildenhain","year":"2015","unstructured":"Wildenhain, J. et al. Prediction of Synergism from Chemical-Genetic Interactions by Machine Learning. Cell Syst. 1 (6), 383-395 (2015).","journal-title":"Cell Syst"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/msb.2011.31","type":"journal-article","article-title":"Cross-species discovery of syncretic drug combinations that potentiate the antifungal fluconazole","volume":"7","author":"Spitzer","year":"2011","unstructured":"Spitzer, M. et al. Cross-species discovery of syncretic drug combinations that potentiate the antifungal fluconazole. Mol Syst Biol. 7 499-499 (2011).","journal-title":"Mol Syst Biol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/video\/57241\/high-throughput-identification-synergistic-drug-combinations-overlap2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:10:10Z","timestamp":1792667410000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/57241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,21]]},"references-count":24,"journal-issue":{"issue":"135","published-online":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.3791\/57241","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,21]]}}}