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Targeted Proteomic Strategy for Clinical Biomarker Discovery. Mol. Oncol. 3, 33-44 (2009).","journal-title":"Mol. Oncol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1177\/2211068215589580","type":"journal-article","article-title":"The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing","volume":"21","author":"Wilson","year":"2016","unstructured":"Wilson, D. H. et al. The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing. J. Lab. Autom. 21, 533-547 (2016).","journal-title":"J. Lab. Autom"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jim.2015.04.017","type":"journal-article","article-title":"A fully-automated, six-plex single molecule immunoassay for measuring cytokines in blood","volume":"424","author":"Rivnak","year":"2015","unstructured":"Rivnak, A. J. et al. 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