{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:43:07Z","timestamp":1792669387672,"version":"4.5.0"},"reference-count":24,"publisher":"MyJove Corporation","issue":"135","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/57642","type":"journal-article","created":{"date-parts":[[2018,5,6]],"date-time":"2018-05-06T20:00:08Z","timestamp":1525636808000},"source":"Crossref","is-referenced-by-count":6,"title":["Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin"],"prefix":"10.3791","author":[{"given":"Bernhard N.","family":"Bohnert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferruh","family":"Artunc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2018,5,6]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1111\/j.1440-1797.2010.01383.x","type":"journal-article","article-title":"Adriamycin nephropathy: a model of focal segmental glomerulosclerosis","volume":"16","author":"Lee","year":"2011","unstructured":"Lee, V. W., & Harris, D. C. Adriamycin nephropathy: a model of focal segmental glomerulosclerosis. Nephrology (Carlton). 16 (1), 30-38 (2011).","journal-title":"Nephrology (Carlton)"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"424","DOI":"10.3181\/00379727-89-21833","type":"journal-article","article-title":"Experimental nephrotic syndrome induced in rats by aminonucleoside; renal lesions and body electrolyte composition","volume":"89","author":"Frenk","year":"1955","unstructured":"Frenk, S., Antonowicz, I., Craig, J. M., & Metcoff, J. Experimental nephrotic syndrome induced in rats by aminonucleoside; renal lesions and body electrolyte composition. Proc Soc Exp Biol Med. 89 (3), 424-427 (1955).","journal-title":"Proc Soc Exp Biol Med"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1172\/JCI110756","type":"journal-article","article-title":"Role for intrarenal mechanisms in the impaired salt excretion of experimental nephrotic syndrome","volume":"71","author":"Ichikawa","year":"1983","unstructured":"Ichikawa, I. et al. Role for intrarenal mechanisms in the impaired salt excretion of experimental nephrotic syndrome. J Clin Invest. 71 (1983).","journal-title":"J Clin Invest"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1681\/ASN.V123598","type":"journal-article","article-title":"Collecting duct is a site of sodium retention in PAN nephrosis: a rationale for amiloride therapy","volume":"12","author":"Deschenes","year":"2001","unstructured":"Deschenes, G., Wittner, M., Stefano, A., Jounier, S., & Doucet, A. Collecting duct is a site of sodium retention in PAN nephrosis: a rationale for amiloride therapy. J Am Soc Nephrol. 12 (3), 598-601 (2001).","journal-title":"J Am Soc Nephrol"},{"issue":"12","key":"ref5","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1681\/ASN.2005040363","type":"journal-article","article-title":"Hyperaldosteronemia and activation of the epithelial sodium channel are not required for sodium retention in puromycin-induced nephrosis","volume":"16","author":"Lourdel","year":"2005","unstructured":"Lourdel, S. et al. Hyperaldosteronemia and activation of the epithelial sodium channel are not required for sodium retention in puromycin-induced nephrosis. J Am Soc Nephrol. 16 (12), 3642-3650 (2005).","journal-title":"J Am Soc Nephrol"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"F208","DOI":"10.1152\/ajprenal.00399.2005","type":"journal-article","article-title":"Increased expression but not targeting of ENaC in adrenalectomized rats with PAN-induced nephrotic syndrome","volume":"291","author":"Seigneux","year":"2006","unstructured":"Seigneux, S., Kim, S. W., Hemmingsen, S. C., Frokiaer, J., & Nielsen, S. Increased expression but not targeting of ENaC in adrenalectomized rats with PAN-induced nephrotic syndrome. Am J Physiol Renal Physiol. 291 (1), F208-217 (2006).","journal-title":"Am J Physiol Renal Physiol"},{"issue":"6","key":"ref7","doi-asserted-by":"crossref","first-page":"F1624","DOI":"10.1152\/ajprenal.00032.2008","type":"journal-article","article-title":"Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome","volume":"295","author":"Artunc","year":"2008","unstructured":"Artunc, F. et al. Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome. Am J Physiol Renal Physiol. 295 (6), F1624-1634 (2008).","journal-title":"Am J Physiol Renal Physiol"},{"issue":"7","key":"ref8","doi-asserted-by":"crossref","first-page":"2502","DOI":"10.1073\/pnas.0409786102","type":"journal-article","article-title":"A Mendelian locus on chromosome 16 determines susceptibility to doxorubicin nephropathy in the mouse","volume":"102","author":"Zheng","year":"2005","unstructured":"Zheng, Z. et al. A Mendelian locus on chromosome 16 determines susceptibility to doxorubicin nephropathy in the mouse. Proc Natl Acad Sci U S A. 102 (7), 2502-2507 (2005).","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ref9","first-page":"193","type":"journal-article","article-title":"Interstrain differences in murine daunomycin-induced nephrosis","volume":"63","author":"Kimura","year":"1993","unstructured":"Kimura, M. et al. Interstrain differences in murine daunomycin-induced nephrosis. Nephron. 63 (2), 193-198 (1993).","journal-title":"Nephron"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1046\/j.1523-1755.2000.00342.x","type":"journal-article","article-title":"Progressive adriamycin nephropathy in mice: sequence of histologic and immunohistochemical events","volume":"58","author":"Wang","year":"2000","unstructured":"Wang, Y., Wang, Y. P., Tay, Y. C., & Harris, D. C. Progressive adriamycin nephropathy in mice: sequence of histologic and immunohistochemical events. Kidney Int. 58 (4), 1797-1804 (2000).","journal-title":"Kidney Int"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1681\/ASN.2008040364","type":"journal-article","article-title":"Plasmin in nephrotic urine activates the epithelial sodium channel","volume":"20","author":"Svenningsen","year":"2009","unstructured":"Svenningsen, P. et al. Plasmin in nephrotic urine activates the epithelial sodium channel. Journal of the American Society of Nephrology : JASN. 20 (2), 299-310 (2009).","journal-title":"Journal of the American Society of Nephrology : JASN"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1097\/MNH.0b013e3283330fb2","type":"journal-article","article-title":"New role for plasmin in sodium homeostasis","volume":"19","author":"Passero","year":"2010","unstructured":"Passero, C. J., Hughey, R. P., & Kleyman, T. R. New role for plasmin in sodium homeostasis. Curr Opin Nephrol Hypertens. 19 (1), 13-19 (2010).","journal-title":"Curr Opin Nephrol Hypertens"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.kint.2017.07.023","type":"journal-article","article-title":"Aprotinin prevents proteolytic epithelial sodium channel (ENaC) activation and volume retention in nephrotic syndrome","volume":"93","author":"Bohnert","year":"2018","unstructured":"Bohnert, B. N. et al. Aprotinin prevents proteolytic epithelial sodium channel (ENaC) activation and volume retention in nephrotic syndrome. Kidney Int. 93 (1), 159-172 (2018).","journal-title":"Kidney Int"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"F213","DOI":"10.1152\/ajprenal.90421.2008","type":"journal-article","article-title":"Inducible rodent models of acquired podocyte diseases","volume":"296","author":"Pippin","year":"2009","unstructured":"Pippin, J. W. et al. Inducible rodent models of acquired podocyte diseases. Am J Physiol Renal Physiol. 296 (2), F213-229 (2009).","journal-title":"Am J Physiol Renal Physiol"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1159\/000368491","type":"journal-article","article-title":"Impact of phosphorus restriction and vitamin D-substitution on secondary hyperparathyroidism in a proteinuric mouse model","volume":"40","author":"Bohnert","year":"2015","unstructured":"Bohnert, B. N. et al. Impact of phosphorus restriction and vitamin D-substitution on secondary hyperparathyroidism in a proteinuric mouse model. Kidney Blood Press Res. 40 (2), 153-165 (2015).","journal-title":"Kidney Blood Press Res"},{"issue":"16","key":"ref16","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1136\/vr.116.16.431","type":"journal-article","article-title":"Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessment","volume":"116","author":"Morton","year":"1985","unstructured":"Morton, D. B., & Griffiths, P. H. Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessment. Vet Rec. 116 (16), 431-436 (1985).","journal-title":"Vet Rec"},{"issue":"2","key":"ref17","first-page":"257","type":"journal-article","article-title":"Plasma aprotinin concentrations during cardiac surgery: full- versus half-dose regimens","volume":"91","author":"Beath","year":"2000","unstructured":"Beath, S. M. et al. Plasma aprotinin concentrations during cardiac surgery: full- versus half-dose regimens. Anesth Analg. 91 (2), 257-264 (2000).","journal-title":"Anesth Analg"},{"issue":"1","key":"ref18","first-page":"85","type":"journal-article","article-title":"Preclinical toxicology study of liposome encapsulated doxorubicin (TLC D-99): comparison with doxorubicin and empty liposomes in mice and dogs","volume":"7","author":"Kanter","year":"1993","unstructured":"Kanter, P. M. et al. Preclinical toxicology study of liposome encapsulated doxorubicin (TLC D-99): comparison with doxorubicin and empty liposomes in mice and dogs. In Vivo. 7 (1), 85-95 (1993).","journal-title":"In Vivo"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1046\/j.1523-1755.2003.00058.x","type":"journal-article","article-title":"Model of robust induction of glomerulosclerosis in mice: importance of genetic background","volume":"64","author":"Ma","year":"2003","unstructured":"Ma, L. J., & Fogo, A. B. Model of robust induction of glomerulosclerosis in mice: importance of genetic background. Kidney Int. 64 (1), 350-355 (2003).","journal-title":"Kidney Int"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1046\/j.1523-1755.1999.00527.x","type":"journal-article","article-title":"The course of the remnant kidney model in mice","volume":"56","author":"Kren","year":"1999","unstructured":"Kren, S., & Hostetter, T. H. The course of the remnant kidney model in mice. Kidney Int. 56 (1), 333-337 (1999).","journal-title":"Kidney Int"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1006\/bbrc.2001.4903","type":"journal-article","article-title":"Advanced glomerulosclerosis is reversible in nephrotic mice","volume":"284","author":"Mizuno-Horikawa","year":"2001","unstructured":"Mizuno-Horikawa, Y., Mizuno, S., Tamura, S., & Kurosawa, T. Advanced glomerulosclerosis is reversible in nephrotic mice. Biochem Biophys Res Commun. 284 (3), 707-713 (2001).","journal-title":"Biochem Biophys Res Commun"},{"key":"ref22","first-page":"1","type":"journal-article","article-title":"Chromogenic peptide substrates. Their use for the assay of factors in the fibrinolytic and the plasma kallikrein-kinin systems","volume":"162","author":"Friberger","year":"1982","unstructured":"Friberger, P. Chromogenic peptide substrates. Their use for the assay of factors in the fibrinolytic and the plasma kallikrein-kinin systems. Scand J Clin Lab Invest Suppl. 162 1-298 (1982).","journal-title":"Scand J Clin Lab Invest Suppl"},{"key":"ref23","doi-asserted-by":"crossref","DOI":"10.1111\/apha.13060","type":"journal-article","article-title":"Plasma kallikrein activates the epithelial sodium channel in vitro but is not essential for volume retention in nephrotic mice","author":"Haerteis","year":"2018","unstructured":"Haerteis, S. et al. Plasma kallikrein activates the epithelial sodium channel (ENaC) in vitro but is not essential for volume retention in nephrotic mice. Acta Physiologica. (2018).","journal-title":"Acta Physiologica"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"761","DOI":"10.2215\/CJN.12261115","type":"journal-article","article-title":"Association of Plasminuria with Overhydration in Patients with CKD","volume":"11","author":"Schork","year":"2016","unstructured":"Schork, A. et al. Association of Plasminuria with Overhydration in Patients with CKD. Clin J Am Soc Nephrol. 11 (5), 761-769 (2016).","journal-title":"Clin J Am Soc Nephrol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/video\/57642\/induction-nephrotic-syndrome-mice-retrobulbar-injection-doxorubicin","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:16:35Z","timestamp":1792667795000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/57642"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,6]]},"references-count":24,"journal-issue":{"issue":"135","published-online":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.3791\/57642","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,6]]}}}