{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:41:29Z","timestamp":1792669289158,"version":"4.5.0"},"reference-count":16,"publisher":"MyJove Corporation","issue":"135","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/57728","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T20:00:08Z","timestamp":1527192008000},"source":"Crossref","is-referenced-by-count":2,"title":["Imaging Ca&lt;sup&gt;2+&lt;\/sup&gt; Responses During &lt;em&gt;Shigella&lt;\/em&gt; Infection of Epithelial Cells"],"prefix":"10.3791","author":[{"given":"Yasmine","family":"Smail","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhui","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Combettes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Tran Van Nhieu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2018,5,24]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/nchembio.741","type":"journal-article","article-title":"Bacteria and host interactions in the gut epithelial barrier","volume":"8","author":"Ashida","year":"2012","unstructured":"Ashida, H., Ogawa, M., Kim, M., Mimuro, H., Sasakawa, C. Bacteria and host interactions in the gut epithelial barrier. Nature Chemical Biology. 8 (1), 36-45 (2012).","journal-title":"Nature Chemical Biology"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/35036035","type":"journal-article","article-title":"The versatility and universality of calcium signaling","volume":"1","author":"Berridge","year":"2000","unstructured":"Berridge, M. J., Lipp, P., Bootman, M. D. The versatility and universality of calcium signaling. Nature Reviews Molecular Cell Biology. 1 (1), 11-21 (2000).","journal-title":"Nature Reviews Molecular Cell Biology"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.bbrc.2015.01.121","type":"journal-article","article-title":"Plasma membrane calcium ATPases: from generic Ca(2+) sump pumps to versatile systems for fine-tuning cellular Ca(2)","volume":"460","author":"Strehler","year":"2015","unstructured":"Strehler, E. E. Plasma membrane calcium ATPases: from generic Ca(2+) sump pumps to versatile systems for fine-tuning cellular Ca(2.). Biochemical and Biophysical Research Communications. 460 (1), 26-33 (2015).","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1083\/jcb.200506047","type":"journal-article","article-title":"Decoding Ca2+ signals: a question of timing","volume":"170","author":"Muallem","year":"2005","unstructured":"Muallem, S. Decoding Ca2+ signals: a question of timing. Journal of Cell Biology. 170 (2), 173-175 (2005).","journal-title":"Journal of Cell Biology"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.bbrc.2010.02.117","type":"journal-article","article-title":"Biochemistry of calcium oscillations","volume":"396","author":"Uhlen","year":"2010","unstructured":"Uhlen, P., Fritz, N. Biochemistry of calcium oscillations. Biochemical and Biophysical Research Communications. 396 (1), 28-32 (2010).","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.chom.2008.12.011","type":"journal-article","article-title":"Shigella induces mitochondrial dysfunction and cell death in nonmyleoid cells","volume":"5","author":"Carneiro","year":"2009","unstructured":"Carneiro, L. A. et al.Shigella induces mitochondrial dysfunction and cell death in nonmyleoid cells. Cell Host & Microbe. 5 (2), 123-136 (2009).","journal-title":"Cell Host & Microbe"},{"issue":"6","key":"ref7","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.it.2014.02.007","type":"journal-article","article-title":"Calcium signaling and mitochondrial destabilization in the triggering of the NLRP3 inflammasome","volume":"35","author":"Horng","year":"2014","unstructured":"Horng, T. Calcium signaling and mitochondrial destabilization in the triggering of the NLRP3 inflammasome. Trends in Immunology. 35 (6), 253-261 (2014).","journal-title":"Trends in Immunology"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1146\/annurev-micro-092412-155725","type":"journal-article","article-title":"Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells","volume":"68","author":"Galan","year":"2014","unstructured":"Galan, J. E., Lara-Tejero, M., Marlovits, T. C., Wagner, S. Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells. Annual Review of Microbiology. 68, 415-438 (2014).","journal-title":"Annual Review of Microbiology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"219","DOI":"10.3389\/fimmu.2015.00219","type":"journal-article","article-title":"Shigella manipulates host immune responses by delivering effector proteins with specific roles","volume":"6","author":"Ashida","year":"2015","unstructured":"Ashida, H., Mimuro, H., Sasakawa, C. Shigella manipulates host immune responses by delivering effector proteins with specific roles. Frontiers in Immunology. 6, 219 (2015).","journal-title":"Frontiers in Immunology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1038\/ncomms2561","type":"journal-article","article-title":"Actin-based confinement of calcium responses during Shigella invasion","volume":"4","author":"Tran Van Nhieu","year":"2013","unstructured":"Tran Van Nhieu, G. et al. Actin-based confinement of calcium responses during Shigella invasion. Nature Communications. 4, 1567 (2013).","journal-title":"Nature Communications"},{"issue":"19","key":"ref11","doi-asserted-by":"crossref","first-page":"5069","DOI":"10.1093\/emboj\/cdf522","type":"journal-article","article-title":"Conversion of PtdIns(4,5)P(2) into PtdIns(5)P by the S. flexneri effector IpgD reorganizes host cell morphology","volume":"21","author":"Niebuhr","year":"2002","unstructured":"Niebuhr, K. et al. Conversion of PtdIns(4,5)P(2) into PtdIns(5)P by the S. flexneri effector IpgD reorganizes host cell morphology. The EMBO Journal. 21 (19), 5069-5078 (2002).","journal-title":"The EMBO Journal"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.chom.2011.03.010","type":"journal-article","article-title":"The Shigella flexneri type three secretion system effector IpgD inhibits T cell migration by manipulating host phosphoinositide metabolism","volume":"9","author":"Konradt","year":"2011","unstructured":"Konradt, C. et al. The Shigella flexneri type three secretion system effector IpgD inhibits T cell migration by manipulating host phosphoinositide metabolism. Cell Host & Microbe. 9 (4), 263-272 (2011).","journal-title":"Cell Host & Microbe"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.bbrc.2004.08.194","type":"journal-article","article-title":"The intriguing Ca2+ requirement of calpain activation","volume":"323","author":"Friedrich","year":"2004","unstructured":"Friedrich, P. The intriguing Ca2+ requirement of calpain activation. Biochemical and Biophysical Research Communications. 323 (4), 1131-1133 (2004).","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1054\/ceca.2000.0152","type":"journal-article","article-title":"A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals","volume":"28","author":"Thomas","year":"2000","unstructured":"Thomas, D. et al. A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals. Cell Calcium. 28 (4), 213-223 (2000).","journal-title":"Cell Calcium"},{"issue":"17","key":"ref15","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.15252\/embj.201696272","type":"journal-article","article-title":"The Shigella type III effector IpgD recodes Ca2+ signals during invasion of epithelial cells","volume":"36","author":"Sun","year":"2017","unstructured":"Sun, C. H. et al. The Shigella type III effector IpgD recodes Ca2+ signals during invasion of epithelial cells. The EMBO Journal. 36 (17), 2567-2580 (2017).","journal-title":"The EMBO Journal"},{"issue":"5","key":"ref16","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1128\/iai.61.5.1707-1714.1993","type":"journal-article","article-title":"Characterization of the Shigella flexneri IpgD and IpgF genes, which are located in the proximal part of the mxi locus","volume":"61","author":"Allaoui","year":"1993","unstructured":"Allaoui, A., Menard, R., Sansonetti, P. J., Parsot, C. Characterization of the Shigella flexneri IpgD and IpgF genes, which are located in the proximal part of the mxi locus. Infection and Immunity. 61 (5), 1707-1714 (1993).","journal-title":"Infection and Immunity"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/video\/57728\/imaging-ca2-responses-during-shigella-infection-of-epithelial-cells","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:09:11Z","timestamp":1792667351000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/57728"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,24]]},"references-count":16,"journal-issue":{"issue":"135","published-online":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.3791\/57728","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,24]]}}}