{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:38:50Z","timestamp":1792636730924,"version":"4.5.0"},"reference-count":31,"publisher":"MyJove Corporation","issue":"141","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/58123","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T19:00:12Z","timestamp":1543258812000},"source":"Crossref","is-referenced-by-count":9,"title":["Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation"],"prefix":"10.3791","author":[{"given":"Doris A.","family":"Taylor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz C.","family":"Sampaio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Cabello","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelmotagaly","family":"Elgalad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohan","family":"Parikh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R Patrick","family":"Wood","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Myer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvin T.","family":"Yeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po-Feng","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2018,11,26]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1161\/CIR.0000000000000366","type":"journal-article","article-title":"Executive Summary: Heart Disease and Stroke Statistics--2016 Update: A Report From the American Heart Association","volume":"133","year":"2016","unstructured":"Writing Group Members, et al. Executive Summary: Heart Disease and Stroke Statistics--2016 Update: A Report From the American Heart Association. Circulation. 133 (4), 447-454, (2016).","journal-title":"Circulation"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"705","DOI":"10.3109\/07388551.2015.1007495","type":"journal-article","article-title":"Hearts beating through decellularized scaffolds: whole-organ engineering for cardiac regeneration and transplantation","volume":"36","author":"Zia","year":"2016","unstructured":"Zia, S. et al. Hearts beating through decellularized scaffolds: whole-organ engineering for cardiac regeneration and transplantation. Critical Reviews in Biotechnology. 36 (4), 705-715, (2016).","journal-title":"Critical Reviews in Biotechnology"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1161\/CIRCRESAHA.115.307938","type":"journal-article","article-title":"Strip and Dress the Human Heart","volume":"118","author":"Zimmermann","year":"2016","unstructured":"Zimmermann, W. H. Strip and Dress the Human Heart. Circulation Research. 118 (1), 12-13, (2016).","journal-title":"Circulation Research"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nm1684","type":"journal-article","article-title":"Perfusion-decellularized matrix: Using nature's platform to engineer a bioartificial heart","volume":"14","author":"Ott","year":"2008","unstructured":"Ott, H. C. et al. Perfusion-decellularized matrix: Using nature's platform to engineer a bioartificial heart. Nature Medicine. 14 (2), 213-221, (2008).","journal-title":"Nature Medicine"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.biomaterials.2015.04.056","type":"journal-article","article-title":"Acellular human heart matrix: A critical step toward whole heart grafts","volume":"61","author":"Sanchez","year":"2015","unstructured":"Sanchez, P. L. et al. Acellular human heart matrix: A critical step toward whole heart grafts. Biomaterials. 61, 279-289, (2015).","journal-title":"Biomaterials"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s13287-015-0089-y","type":"journal-article","article-title":"Current achievements and future perspectives in whole-organ bioengineering","volume":"6","author":"Peloso","year":"2015","unstructured":"Peloso, A. et al. Current achievements and future perspectives in whole-organ bioengineering. Stem Cell Research & Therapy. 6, 107, (2015).","journal-title":"Stem Cell Research & Therapy"},{"issue":"6","key":"ref7","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1038\/nprot.2014.097","type":"journal-article","article-title":"Perfusion decellularization of whole organs","volume":"9","author":"Guyette","year":"2014","unstructured":"Guyette, J. P. et al. Perfusion decellularization of whole organs. Nature Protocols. 9 (6), 1451-1468, (2014).","journal-title":"Nature Protocols"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1089\/ten.teb.2014.0192","type":"journal-article","article-title":"Strategies and Processes to Decellularize and Cellularize Hearts to Generate Functional Organs and Reduce the Risk of Thrombosis","volume":"21","author":"Momtahan","year":"2015","unstructured":"Momtahan, N., Sukavaneshvar, S., Roeder, B. L., & Cook, A. D. Strategies and Processes to Decellularize and Cellularize Hearts to Generate Functional Organs and Reduce the Risk of Thrombosis. Tissue Engineering Part B-Reviews. 21 (1), 115-132, (2015).","journal-title":"Tissue Engineering Part B-Reviews"},{"key":"ref9","type":"journal-article","article-title":"Inverted orientation improves decellularization of whole porcine hearts","author":"Lee","year":"2016","unstructured":"Lee, P. F. et al. Inverted orientation improves decellularization of whole porcine hearts. Acta Biomaterialia. (2016).","journal-title":"Acta Biomaterialia"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1089\/ten.tec.2014.0709","type":"journal-article","article-title":"Automation of Pressure Control Improves Whole Porcine Heart Decellularization","author":"Momtahan","year":"2015","unstructured":"Momtahan, N. et al. Automation of Pressure Control Improves Whole Porcine Heart Decellularization. Tissue Eng Part C Methods. (2015).","journal-title":"Tissue Eng Part C Methods"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1253\/circj.CJ-10-0717","type":"journal-article","article-title":"Development and Evaluation of a Perfusion Decellularization Porcine Heart Model - Generation of 3-Dimensional Myocardial Neoscaffolds","volume":"75","author":"Weymann","year":"2011","unstructured":"Weymann, A. et al. Development and Evaluation of a Perfusion Decellularization Porcine Heart Model - Generation of 3-Dimensional Myocardial Neoscaffolds. Circulation Journal. 75 (4), 852-860, (2011).","journal-title":"Circulation Journal"},{"issue":"11","key":"ref12","doi-asserted-by":"crossref","first-page":"e111591","DOI":"10.1371\/journal.pone.0111591","type":"journal-article","article-title":"Bioartificial heart: A human-sized porcine model--the way ahead","volume":"9","author":"Weymann","year":"2014","unstructured":"Weymann, A. et al. Bioartificial heart: A human-sized porcine model--the way ahead. PLoS One. 9 (11), e111591, (2014).","journal-title":"PLoS One"},{"issue":"70","key":"ref13","first-page":"e50059","type":"journal-article","article-title":"Procedure for decellularization of porcine heart by retrograde coronary perfusion","author":"Remlinger","year":"2012","unstructured":"Remlinger, N. T., Wearden, P. D., & Gilbert, T. W. Procedure for decellularization of porcine heart by retrograde coronary perfusion. Journal of Visualized Experiments. (70), e50059, (2012).","journal-title":"Journal of Visualized Experiments"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1161\/CIRCRESAHA.115.306874","type":"journal-article","article-title":"Bioengineering Human Myocardium on Native Extracellular Matrix","volume":"118","author":"Guyette","year":"2016","unstructured":"Guyette, J. P. et al. Bioengineering Human Myocardium on Native Extracellular Matrix. Circulation Research. 118 (1), 56-72, (2016).","journal-title":"Circulation Research"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.dib.2016.04.069","type":"journal-article","article-title":"Data from acellular human heart matrix","volume":"8","author":"Sanchez","year":"2016","unstructured":"Sanchez, P. L. et al. Data from acellular human heart matrix. Data Brief. 8, 211-219, (2016).","journal-title":"Data Brief"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.biomaterials.2016.04.003","type":"journal-article","article-title":"Myocardial commitment from human pluripotent stem cells: Rapid production of human heart grafts","volume":"98","author":"Garreta","year":"2016","unstructured":"Garreta, E. et al. Myocardial commitment from human pluripotent stem cells: Rapid production of human heart grafts. Biomaterials. 98, 64-78, (2016).","journal-title":"Biomaterials"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1089\/ten.tec.2009.0392","type":"journal-article","article-title":"Preparation of Cardiac Extracellular Matrix from an Intact Porcine Heart","volume":"16","author":"Wainwright","year":"2010","unstructured":"Wainwright, J. M. et al. Preparation of Cardiac Extracellular Matrix from an Intact Porcine Heart. Tissue Engineering Part C-Methods. 16 (3), 525-532, (2010).","journal-title":"Tissue Engineering Part C-Methods"},{"issue":"11","key":"ref18","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1364\/OL.31.001681","type":"journal-article","article-title":"Ex vivo characterization of sub-10-fs pulses","volume":"31","author":"Larson","year":"2006","unstructured":"Larson, A. M., & Yeh, A. T. Ex vivo characterization of sub-10-fs pulses. Optics Letters. 31 (11), 1681-1683, (2006).","journal-title":"Optics Letters"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.yexcr.2008.10.040","type":"journal-article","article-title":"Nonlinear optical microscopy reveals invading endothelial cells anisotropically alter three-dimensional collagen matrices","volume":"315","author":"Lee","year":"2009","unstructured":"Lee, P. F., Yeh, A. T., & Bayless, K. J. Nonlinear optical microscopy reveals invading endothelial cells anisotropically alter three-dimensional collagen matrices. Experimental Cell Research. 315 (3), 396-410, (2009).","journal-title":"Experimental Cell Research"},{"issue":"7","key":"ref20","doi-asserted-by":"crossref","first-page":"7178","DOI":"10.1016\/j.actbio.2013.04.001","type":"journal-article","article-title":"Angiogenic responses are enhanced in mechanically and microscopically characterized, microbial transglutaminase crosslinked collagen matrices with increased stiffness","volume":"9","author":"Lee","year":"2013","unstructured":"Lee, P. F., Bai, Y., Smith, R. L., Bayless, K. J., & Yeh, A. T. Angiogenic responses are enhanced in mechanically and microscopically characterized, microbial transglutaminase crosslinked collagen matrices with increased stiffness. Acta Biomaterialia. 9 (7), 7178-7190, (2013).","journal-title":"Acta Biomaterialia"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ymeth.2017.04.013","type":"journal-article","article-title":"Multi-photon microscopy in cardiovascular research","volume":"130","author":"Wu","year":"2017","unstructured":"Wu, Z. et al. Multi-photon microscopy in cardiovascular research. Methods. 130, 79-89, (2017).","journal-title":"Methods"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/bjaceaccp\/mki012","type":"journal-article","article-title":"Coronary blood flow","volume":"5","author":"Ramanathan","year":"2005","unstructured":"Ramanathan, T., Skinner, H. Coronary blood flow. Continuing Education in Anaesthesia Critical Care & Pain. 5 (2), 61-64, (2005).","journal-title":"Continuing Education in Anaesthesia Critical Care & Pain"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s12350-017-1110-x","type":"journal-article","article-title":"Clinical Quantification of Myocardial Blood Flow Using PET: Joint Position Paper of the SNMMI Cardiovascular Council and the ASNC","volume":"25","author":"Murthy","year":"2018","unstructured":"Murthy, V. L. et al. Clinical Quantification of Myocardial Blood Flow Using PET: Joint Position Paper of the SNMMI Cardiovascular Council and the ASNC. Journal of Nuclear Cardiology. 25 (1), 269-297, (2018).","journal-title":"Journal of Nuclear Cardiology"},{"issue":"4","key":"ref24","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1097\/PAF.0b013e31823d298b","type":"journal-article","article-title":"Normal organ weights in men: Part I-the heart","volume":"33","author":"Molina","year":"2012","unstructured":"Molina, D. K., & DiMaio, V. J. Normal organ weights in men: Part I-the heart. The American Journal of Forensic Medicine and Pathology. 33 (4), 362-367, (2012).","journal-title":"The American Journal of Forensic Medicine and Pathology"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1097\/PAF.0000000000000174","type":"journal-article","article-title":"Normal Organ Weights in Women: Part I-The Heart","volume":"36","author":"Molina","year":"2015","unstructured":"Molina, D. K., & DiMaio, V. J. Normal Organ Weights in Women: Part I-The Heart. The American Journal of Forensic Medicine and Pathology. 36 (3), 176-181, (2015).","journal-title":"The American Journal of Forensic Medicine and Pathology"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"e90406","DOI":"10.1371\/journal.pone.0090406","type":"journal-article","article-title":"Optimizing cellularization of whole decellularized heart extracellular matrix","volume":"9","author":"Robertson","year":"2014","unstructured":"Robertson, M. J., Dries-Devlin, J. L., Kren, S. M., Burchfield, J. S., & Taylor, D. A. Optimizing cellularization of whole decellularized heart extracellular matrix. PLoS One. 9 (2), e90406, (2014).","journal-title":"PLoS One"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"e0191892","DOI":"10.1371\/journal.pone.0191892","type":"journal-article","article-title":"Cellularization of rat liver: An in vitro model for assessing human drug metabolism and liver biology","volume":"13","author":"Robertson","year":"2018","unstructured":"Robertson, M. J., Soibam, B., O'Leary, J. G., Sampaio, L. C., & Taylor, D. A. Cellularization of rat liver: An in vitro model for assessing human drug metabolism and liver biology. PLoS One. 13 (1), e0191892, (2018).","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2018.05.147","type":"journal-article","article-title":"Cardiac extracellular matrix hydrogel together with or without inducer cocktail improves human adipose tissue-derived stem cells differentiation into cardiomyocyte-like cells","author":"Baghalishahi","year":"2018","unstructured":"Baghalishahi, M. et al. Cardiac extracellular matrix hydrogel together with or without inducer cocktail improves human adipose tissue-derived stem cells differentiation into cardiomyocyte-like cells. Biochemical and Biophysical Research Communications. (2018).","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"3","key":"ref29","first-page":"558","type":"journal-article","article-title":"In vitro comparative study of two decellularization protocols in search of an optimal myocardial scaffold for recellularization","volume":"7","author":"Perea-Gil","year":"2015","unstructured":"Perea-Gil, I. et al. In vitro comparative study of two decellularization protocols in search of an optimal myocardial scaffold for cellularization. American Journal of Translational Research. 7 (3), 558-573, (2015).","journal-title":"American Journal of Translational Research"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/978-1-4939-1047-2_7","type":"journal-article","article-title":"Natural cardiac extracellular matrix hydrogels for cultivation of human stem cell-derived cardiomyocytes","volume":"1181","author":"Freytes","year":"2014","unstructured":"Freytes, D. O., O'Neill, J. D., Duan-Arnold, Y., Wrona, E. A., & Vunjak-Novakovic, G. Natural cardiac extracellular matrix hydrogels for cultivation of human stem cell-derived cardiomyocytes. Methods Molecular Biology. 1181, 69-81, (2014).","journal-title":"Methods Molecular Biology"},{"issue":"9","key":"ref31","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.1002\/jbm.a.35000","type":"journal-article","article-title":"Preparation of cardiac extracellular matrix scaffolds by decellularization of human myocardium","volume":"102","author":"Oberwallner","year":"2014","unstructured":"Oberwallner, B. et al. Preparation of cardiac extracellular matrix scaffolds by decellularization of human myocardium. Journal of Biomedical Materials Research Part A. 102 (9), 3263-3272, (2014).","journal-title":"Journal of Biomedical Materials Research Part A"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/58123\/decellularization-whole-human-heart-inside-pressurized-pouch-an","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:07:15Z","timestamp":1792634835000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/58123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,26]]},"references-count":31,"journal-issue":{"issue":"141","published-online":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.3791\/58123","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,26]]}}}