{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:37:52Z","timestamp":1792665472168,"version":"4.5.0"},"reference-count":15,"publisher":"MyJove Corporation","issue":"141","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/58450","type":"journal-article","created":{"date-parts":[[2018,11,17]],"date-time":"2018-11-17T19:00:57Z","timestamp":1542481257000},"source":"Crossref","is-referenced-by-count":8,"title":["<em>In Vivo<\/em> Imaging of Reactive Oxygen Species in a Murine Wound Model"],"prefix":"10.3791","author":[{"given":"Piul S.","family":"Rabbani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma A.","family":"Abdou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren L.","family":"Sultan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Kwong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April","family":"Duckworth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Ceradini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2018,11,17]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1006\/bbrc.1993.1659","type":"journal-article","article-title":"A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells","volume":"193","author":"Nishinaka","year":"1993","unstructured":"Nishinaka, Y. et al. A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells. Biochemical and Biophysical Research Communications. 193 (2), 554-559, (1993).","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.freeradbiomed.2003.10.012","type":"journal-article","article-title":"Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012","volume":"36","author":"Daiber","year":"2004","unstructured":"Daiber, A. et al. Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012. Free Radical Biology and Medicine. 36 (1), 101-111, (2004).","journal-title":"Free Radical Biology and Medicine"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1006\/abio.1999.4107","type":"journal-article","article-title":"Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012","volume":"271","author":"Imada","year":"1999","unstructured":"Imada, I. et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012. Analytical Biochemistry. 271 (1), 53-58, (1999).","journal-title":"Analytical Biochemistry"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1159\/000025688","type":"journal-article","article-title":"Sensitive superoxide detection in vascular cells by the new chemiluminescence dye L-012","volume":"36","author":"Sohn","year":"1999","unstructured":"Sohn, H. Y., Gloe, T., Keller, M., Schoenafinger, K., & Pohl, U. Sensitive superoxide detection in vascular cells by the new chemiluminescence dye L-012. Journal of Vascular Research. 36 (6), 456-464, (1999).","journal-title":"Journal of Vascular Research"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"853","DOI":"10.2967\/jnumed.116.185934","type":"journal-article","article-title":"In vivo Hypoxia PET Imaging Quantifies the Severity of Arthritic Joint Inflammation in Line with Overexpression of Hypoxia-Inducible Factor and Enhanced Reactive Oxygen Species Generation","volume":"58","author":"Fuchs","year":"2017","unstructured":"Fuchs, K. et al. In vivo Hypoxia PET Imaging Quantifies the Severity of Arthritic Joint Inflammation in Line with Overexpression of Hypoxia-Inducible Factor and Enhanced Reactive Oxygen Species Generation. The Journal of Nuclear Medicine. 58 (5), 853-860, (2017).","journal-title":"The Journal of Nuclear Medicine"},{"issue":"8","key":"ref6","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1097\/MIB.0000000000000118","type":"journal-article","article-title":"In vivo imaging of reactive oxygen and nitrogen species in murine colitis","volume":"20","author":"Asghar","year":"2014","unstructured":"Asghar, M. N. et al. In vivo imaging of reactive oxygen and nitrogen species in murine colitis. Inflammatory Bowel Diseases. 20 (8), 1435-1447, (2014).","journal-title":"Inflammatory Bowel Diseases"},{"issue":"4","key":"ref7","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1111\/j.1067-1927.2004.12404.x","type":"journal-article","article-title":"Quantitative and reproducible murine model of excisional wound healing","volume":"12","author":"Galiano","year":"2004","unstructured":"Galiano, R. D., Michaels, J. t., Dobryansky, M., Levine, J. P., & Gurtner, G. C. Quantitative and reproducible murine model of excisional wound healing. Wound Repair and Regeneration. 12 (4), 485-492, (2004).","journal-title":"Wound Repair and Regeneration"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"633","DOI":"10.2337\/db15-0453","type":"journal-article","article-title":"Restoration of Nrf2 Signaling Normalizes the Regenerative Niche","volume":"65","author":"Soares","year":"2016","unstructured":"Soares, M. A. et al. Restoration of Nrf2 Signaling Normalizes the Regenerative Niche. Diabetes. 65 (3), 633-646, (2016).","journal-title":"Diabetes"},{"issue":"8","key":"ref9","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1007\/s00109-013-1067-4","type":"journal-article","article-title":"Imaging ROS signaling in cells and animals","volume":"91","author":"Wang","year":"2013","unstructured":"Wang, X. et al. Imaging ROS signaling in cells and animals. Journal of Molecular Medicine. 91 (8), 917-927, (2013).","journal-title":"Journal of Molecular Medicine"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/j.freeradbiomed.2009.06.013","type":"journal-article","article-title":"In vivo imaging of reactive oxygen and nitrogen species in inflammation using the luminescent probe L-012","volume":"47","author":"Kielland","year":"2009","unstructured":"Kielland, A. et al. In vivo imaging of reactive oxygen and nitrogen species in inflammation using the luminescent probe L-012. Free Radical Biology and Medicine. 47 (6), 760-766, (2009).","journal-title":"Free Radical Biology and Medicine"},{"key":"ref11","type":"journal-article","article-title":"Visualizing Oxidative Cellular Stress Induced by Nanoparticles in the Subcytotoxic Range Using Fluorescence Lifetime Imaging","author":"Balke","year":"2018","unstructured":"Balke, J. et al. Visualizing Oxidative Cellular Stress Induced by Nanoparticles in the Subcytotoxic Range Using Fluorescence Lifetime Imaging. Small. e1800310, (2018).","journal-title":"Small"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1016\/j.freeradbiomed.2013.09.017","type":"journal-article","article-title":"On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation","volume":"65","author":"Zielonka","year":"2013","unstructured":"Zielonka, J., Lambeth, J. D., & Kalyanaraman, B. On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation. Free Radical Biology and Medicine. 65 1310-1314, (2013).","journal-title":"Free Radical Biology and Medicine"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1089\/ars.2012.4886","type":"journal-article","article-title":"Methods for detection of mitochondrial and cellular reactive oxygen species","volume":"20","author":"Dikalov","year":"2014","unstructured":"Dikalov, S. I., & Harrison, D. G. Methods for detection of mitochondrial and cellular reactive oxygen species. Antioxidants & Redox Signalling. 20 (2), 372-382, (2014).","journal-title":"Antioxidants & Redox Signalling"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.diabres.2018.02.021","type":"journal-article","article-title":"Targeted Nrf2 activation therapy with RTA 408 enhances regenerative capacity of diabetic wounds","volume":"139","author":"Rabbani","year":"2018","unstructured":"Rabbani, P. S. et al. Targeted Nrf2 activation therapy with RTA 408 enhances regenerative capacity of diabetic wounds. Diabetes Research and Clinical Practice. 139 11-23, (2018).","journal-title":"Diabetes Research and Clinical Practice"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biomaterials.2017.04.001","type":"journal-article","article-title":"Novel lipoproteoplex delivers Keap1 siRNA based gene therapy to accelerate diabetic wound healing","volume":"132","author":"Rabbani","year":"2017","unstructured":"Rabbani, P. S. et al. Novel lipoproteoplex delivers Keap1 siRNA based gene therapy to accelerate diabetic wound healing. Biomaterials. 132 1-15, (2017).","journal-title":"Biomaterials"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/58450\/in-vivo-imaging-of-reactive-oxygen-species-in-a-murine-wound-model","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:41:06Z","timestamp":1792662066000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/58450"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,17]]},"references-count":15,"journal-issue":{"issue":"141","published-online":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.3791\/58450","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,17]]}}}