{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:39:48Z","timestamp":1792633188974,"version":"4.5.0"},"reference-count":13,"publisher":"MyJove Corporation","issue":"147","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/59428","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T20:00:18Z","timestamp":1556827218000},"source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses"],"prefix":"10.3791","author":[{"given":"Yuyang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidi","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huancheng","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Gong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongzhong","family":"Tu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchun","family":"Tu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2019,5,2]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S1474-4422(02)00041-8","type":"journal-article","article-title":"Human rabies: a disease of complex neuropathogenetic mechanisms and diagnostic challenges","volume":"1","author":"Hemachudha","year":"2002","unstructured":"Hemachudha, T., Laothamatas, J., Rupprecht, C. E. Human rabies: a disease of complex neuropathogenetic mechanisms and diagnostic challenges. Lancet Neurology. 1 (2), 101-109 (2002).","journal-title":"Lancet Neurology"},{"issue":"8","key":"ref2","doi-asserted-by":"crossref","first-page":"2283","DOI":"10.1007\/s00705-018-3814-x","type":"journal-article","article-title":"Taxonomy of the order Mononegavirales: update 2018","volume":"163","author":"Amarasinghe","year":"2018","unstructured":"Amarasinghe, G. K., Arechiga Ceballos, N. G. Taxonomy of the order Mononegavirales: update 2018. Archives of Virology. 163 (8), 2283-2294, 10.1007\/s00705-018-3814-x (2018).","journal-title":"Archives of Virology"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1111\/tbed.12833","type":"journal-article","article-title":"Tentative novel lyssavirus in a bat in Finland","volume":"65","author":"Nokireki","year":"2018","unstructured":"Nokireki, T., Tammiranta, N., Kokkonen, U. M., Kantala, T., Gadd, T. Tentative novel lyssavirus in a bat in Finland. Transboundary and Emerging Diseases. 65 (3), 593-596 (2018).","journal-title":"Transboundary and Emerging Diseases"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"782","DOI":"10.3201\/eid2404.171696","type":"journal-article","article-title":"Lyssavirus in Japanese Pipistrelle, Taiwan","volume":"24","author":"Hu","year":"2018","unstructured":"Hu, S. C. et al. Lyssavirus in Japanese Pipistrelle, Taiwan. Emerging Infectious Diseases. 24 (4), 782-785 (2018).","journal-title":"Emerging Infectious Diseases"},{"key":"ref5","article-title":"World Organization for Animal Health (OIE). Chapter 2.1.17 - Rabies (infection with rabies virus and other lyssaviruses)","volume-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2018","year":"2018","unstructured":"World Organization for Animal Health (OIE). Chapter 2.1.17 - Rabies (infection with rabies virus and other lyssaviruses), 2-14. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2018. http:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/2.01.17_RABIES.pdf (2018)."},{"key":"ref6","first-page":"74","type":"journal-article","article-title":"Laboratory techniques in rabies","author":"Rupprecht","year":"2018","unstructured":"Rupprecht, C. E., Fooks, A. R., Abela-Ridder, B. Laboratory techniques in rabies. 74-195, World Health Organization (2018).","journal-title":"World Health Organization"},{"issue":"10","key":"ref7","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1002\/jmv.23814","type":"journal-article","article-title":"Utility of real-time Taqman PCR for antemortem and postmortem diagnosis of human rabies","volume":"86","author":"Mani","year":"2014","unstructured":"Mani, R. S. et al. Utility of real-time Taqman PCR for antemortem and postmortem diagnosis of human rabies. Journal of Medical Virology. 86 (10), 1804-1812 (2014).","journal-title":"Journal of Medical Virology"},{"issue":"9","key":"ref8","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1371\/journal.pntd.0000530","type":"journal-article","article-title":"Emerging technologies for the detection of rabies virus: challenges and hopes in the 21st century","volume":"3","author":"Fooks","year":"2009","unstructured":"Fooks, A. R. et al. Emerging technologies for the detection of rabies virus: challenges and hopes in the 21st century. PLOS Neglected Tropical Diseases. 3 (9), e530 (2009).","journal-title":"PLOS Neglected Tropical Diseases"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1017\/S0950268807008874","type":"journal-article","article-title":"An outbreak of pig rabies in Hunan province, China","volume":"136","author":"Jiang","year":"2008","unstructured":"Jiang, Y. et al. An outbreak of pig rabies in Hunan province, China. Epidemiology and Infection. 136 (4), 504-508 (2008).","journal-title":"Epidemiology and Infection"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.ympev.2013.07.008","type":"journal-article","article-title":"Analysis of the complete genome of the first Irkut virus isolate from China: comparison across the Lyssavirus genus","volume":"69","author":"Liu","year":"2013","unstructured":"Liu, Y. et al. Analysis of the complete genome of the first Irkut virus isolate from China: comparison across the Lyssavirus genus. Molecular Phylogenetics and Evolution. 69 (3), 687-693 (2013).","journal-title":"Molecular Phylogenetics and Evolution"},{"issue":"2","key":"ref11","first-page":"146","type":"journal-article","article-title":"Possible Transmission of Irkut Virus from Dogs to Humans","volume":"31","author":"Chen","year":"2018","unstructured":"Chen, T. et al. Possible Transmission of Irkut Virus from Dogs to Humans. Biomedical and Environmental Sciences. 31 (2), 146-148 (2018).","journal-title":"Biomedical and Environmental Sciences"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1017\/S0950268814001952","type":"journal-article","article-title":"Disease outbreaks caused by steppe-type rabies viruses in China","volume":"143","author":"Feng","year":"2015","unstructured":"Feng, Y. et al. Disease outbreaks caused by steppe-type rabies viruses in China. Epidemiology and Infection. 143 (6), 1287-1291 (2015).","journal-title":"Epidemiology and Infection"},{"issue":"10","key":"ref13","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.1007\/s00705-016-2982-9","type":"journal-article","article-title":"Livestock rabies outbreaks in Shanxi province, China","volume":"161","author":"Feng","year":"2016","unstructured":"Feng, Y. et al. Livestock rabies outbreaks in Shanxi province, China. Archives of Virology. 161 (10), 2851-2854 (2016).","journal-title":"Archives of Virology"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/59428\/evaluation-universal-nested-reverse-transcription-polymerase-chain","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:07:09Z","timestamp":1792631229000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/59428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,2]]},"references-count":13,"journal-issue":{"issue":"147","published-online":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.3791\/59428","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,2]]}}}