{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:39:02Z","timestamp":1792633142348,"version":"4.5.0"},"reference-count":23,"publisher":"MyJove Corporation","issue":"149","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/59452","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T20:00:24Z","timestamp":1562788824000},"source":"Crossref","is-referenced-by-count":5,"title":["Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity"],"prefix":"10.3791","author":[{"given":"Meng","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick  J.","family":"Ernst","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asher","family":"Kahn-Krell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengming","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Pretorius","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxi","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Lou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lufang","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuqiang","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2019,7,10]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1093\/eurheartj\/ehu192","type":"journal-article","article-title":"Towards a clinical use of human embryonic stem cell-derived cardiac progenitors: a translational experience","volume":"36","author":"Menasche","year":"2015","unstructured":"Menasche, P. et al. Towards a clinical use of human embryonic stem cell-derived cardiac progenitors: a translational experience. European Heart Journal. 36 (12), 743-750 (2015).","journal-title":"European Heart Journal"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.stem.2011.12.013","type":"journal-article","article-title":"Production of de novo cardiomyocytes: human pluripotent stem cell differentiation and direct reprogramming","volume":"10","author":"Burridge","year":"2012","unstructured":"Burridge, P. W., Keller, G., Gold, J. D., Wu, J. C. Production of de novo cardiomyocytes: human pluripotent stem cell differentiation and direct reprogramming. Cell Stem Cell. 10 (1), 16-28 (2012).","journal-title":"Cell Stem Cell"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1083\/jcb.124.4.619","type":"journal-article","article-title":"Disruption of epithelial cell-matrix interactions induces apoptosis","volume":"124","author":"Frisch","year":"1994","unstructured":"Frisch, S. M., Francis, H. Disruption of epithelial cell-matrix interactions induces apoptosis. Journal of Cell Biology. 124 (4), 619-626 (1994).","journal-title":"Journal of Cell Biology"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"3524","DOI":"10.1038\/s41467-018-05850-w","type":"journal-article","article-title":"Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin","volume":"9","author":"Haun","year":"2018","unstructured":"Haun, F. et al. Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin. Nature Communications. 9 (1), 3524 (2018).","journal-title":"Nature Communications"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1074\/jbc.275.5.3577","type":"journal-article","article-title":"Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop","volume":"275","author":"Ohashi","year":"2000","unstructured":"Ohashi, K. et al. Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop. Journal of Biological Chemistry. 275 (5), 3577-3582 (2000).","journal-title":"Journal of Biological Chemistry"},{"issue":"56","key":"ref6","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1098\/rsif.2010.0419","type":"journal-article","article-title":"Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions","volume":"8","author":"Katoh","year":"2011","unstructured":"Katoh, K., Kano, Y., Noda, Y. Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions. Journal of The Royal Society Interface. 8 (56), 305-311 (2011).","journal-title":"Journal of The Royal Society Interface"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1016\/j.bbamcr.2013.06.026","type":"journal-article","article-title":"Anoikis molecular pathways and its role in cancer progression","volume":"1833","author":"Paoli","year":"2013","unstructured":"Paoli, P., Giannoni, E., Chiarugi, P. Anoikis molecular pathways and its role in cancer progression. Biochimica et Biophysica Acta. 1833 (12), 3481-3498 (2013).","journal-title":"Biochimica et Biophysica Acta"},{"issue":"Pt 2","key":"ref8","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1242\/jcs.041624","type":"journal-article","article-title":"Mechanisms that regulate adaptor binding to beta-integrin cytoplasmic tails","volume":"122","author":"Legate","year":"2009","unstructured":"Legate, K. R., Fassler, R. Mechanisms that regulate adaptor binding to beta-integrin cytoplasmic tails. Journal of Cell Science. 122 (Pt 2), 187-198 (2009).","journal-title":"Journal of Cell Science"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/nbt1310","type":"journal-article","article-title":"A ROCK inhibitor permits survival of dissociated human embryonic stem cells","volume":"25","author":"Watanabe","year":"2007","unstructured":"Watanabe, K. et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nature Biotechnology. 25 (6), 681-686 (2007).","journal-title":"Nature Biotechnology"},{"issue":"8","key":"ref10","doi-asserted-by":"crossref","first-page":"e12148","DOI":"10.1371\/journal.pone.0012148","type":"journal-article","article-title":"The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers","volume":"5","author":"Emre","year":"2010","unstructured":"Emre, N. et al. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. PLoS One. 5 (8), e12148 (2010).","journal-title":"PLoS One"},{"issue":"9","key":"ref11","doi-asserted-by":"crossref","first-page":"637","DOI":"10.2174\/1566524018666180316150936","type":"journal-article","article-title":"ROCK Inhibitor Y-27632 Promotes Human Retinal Pigment Epithelium Survival by Altering Cellular Biomechanical Properties","volume":"17","author":"Ni","year":"2017","unstructured":"Ni, Y., Qin, Y., Fang, Z., Zhang, Z. ROCK Inhibitor Y-27632 Promotes Human Retinal Pigment Epithelium Survival by Altering Cellular Biomechanical Properties. Current Molecular Medicine. 17 (9), 637-646 (2017).","journal-title":"Current Molecular Medicine"},{"issue":"9","key":"ref12","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1038\/nbt1327","type":"journal-article","article-title":"Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts","volume":"25","author":"Laflamme","year":"2007","unstructured":"Laflamme, M. A. et al. Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nature Biotechnology. 25 (9), 1015-1024 (2007).","journal-title":"Nature Biotechnology"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1161\/CIRCRESAHA.117.311504","type":"journal-article","article-title":"CCND2 Overexpression Enhances the Regenerative Potency of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Remuscularization of Injured Ventricle","volume":"122","author":"Zhu","year":"2018","unstructured":"Zhu, W., Zhao, M., Mattapally, S., Chen, S., Zhang, J. CCND2 Overexpression Enhances the Regenerative Potency of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Remuscularization of Injured Ventricle. Circulation Research. 122 (1), 88-96 (2018).","journal-title":"Circulation Research"},{"issue":"7400","key":"ref14","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/nature11139","type":"journal-article","article-title":"Heart repair by reprogramming non-myocytes with cardiac transcription factors","volume":"485","author":"Song","year":"2012","unstructured":"Song, K. et al. Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature. 485 (7400), 599-604 (2012).","journal-title":"Nature"},{"issue":"6","key":"ref15","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.stem.2014.11.009","type":"journal-article","article-title":"Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells","volume":"15","author":"Ye","year":"2014","unstructured":"Ye, L. et al. Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells. Cell Stem Cell. 15 (6), 750-761 (2014).","journal-title":"Cell Stem Cell"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cmet.2016.03.001","type":"journal-article","article-title":"Glutamine Oxidation Is Indispensable for Survival of Human Pluripotent Stem Cells","volume":"23","author":"Tohyama","year":"2016","unstructured":"Tohyama, S. et al. Glutamine Oxidation Is Indispensable for Survival of Human Pluripotent Stem Cells. Cell Metabolism. 23 (4), 663-674 (2016).","journal-title":"Cell Metabolism"},{"issue":"8","key":"ref17","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1161\/CIRCULATIONAHA.114.015231","type":"journal-article","article-title":"Microfluidic Single-Cell Analysis of Transplanted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes After Acute Myocardial Infarction","volume":"132","author":"Ong","year":"2015","unstructured":"Ong, S. G. et al. Microfluidic Single-Cell Analysis of Transplanted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes After Acute Myocardial Infarction. Circulation. 132 (8), 762-771 (2015).","journal-title":"Circulation"},{"key":"ref18","type":"journal-article","article-title":"Y-27632 Preconditioning Enhances Transplantation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Myocardial Infarction Mice","author":"Zhao","year":"2018","unstructured":"Zhao, M. et al. Y-27632 Preconditioning Enhances Transplantation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Myocardial Infarction Mice. Cardiovascular Research. (2018).","journal-title":"Cardiovascular Research"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.stem.2012.09.013","type":"journal-article","article-title":"Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes","volume":"12","author":"Tohyama","year":"2013","unstructured":"Tohyama, S. et al. Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes. Cell Stem Cell. 12 (1), 127-137 (2013).","journal-title":"Cell Stem Cell"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e1012","DOI":"10.1038\/cddis.2013.543","type":"journal-article","article-title":"Integrin inhibition promotes atypical anoikis in glioma cells","volume":"5","author":"Silginer","year":"2014","unstructured":"Silginer, M., Weller, M., Ziegler, U., Roth, P. Integrin inhibition promotes atypical anoikis in glioma cells. Cell Death & Disease. 5 e1012 (2014).","journal-title":"Cell Death & Disease"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"e33206","DOI":"10.1371\/journal.pone.0033206","type":"journal-article","article-title":"N-cadherin mediates neuronal cell survival through Bim down-regulation","volume":"7","author":"Lelievre","year":"2012","unstructured":"Lelievre, E. C. et al. N-cadherin mediates neuronal cell survival through Bim down-regulation. PLoS One. 7 (3), e33206 (2012).","journal-title":"PLoS One"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1093\/jrr\/rru002","type":"journal-article","article-title":"Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lung adenocarcinoma A549 cells","volume":"55","author":"Murata","year":"2014","unstructured":"Murata, K. et al. Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lung adenocarcinoma A549 cells. Journal of Radiation Research. 55 (4), 658-664 (2014).","journal-title":"Journal of Radiation Research"},{"issue":"12","key":"ref23","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1038\/jcbfm.2013.151","type":"journal-article","article-title":"PKC-beta exacerbates in vitro brain barrier damage in hyperglycemic settings via regulation of RhoA\/Rho-kinase\/MLC2 pathway","volume":"33","author":"Srivastava","year":"2013","unstructured":"Srivastava, K., Shao, B., Bayraktutan, U. PKC-beta exacerbates in vitro brain barrier damage in hyperglycemic settings via regulation of RhoA\/Rho-kinase\/MLC2 pathway. Journal of Cerebral Blood Flow & Metabolism. 33 (12), 1928-1936 (2013).","journal-title":"Journal of Cerebral Blood Flow & Metabolism"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/59452\/enhancing-engraftment-human-induced-pluripotent-stem-cell-derived","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:44:18Z","timestamp":1792629858000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/59452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,10]]},"references-count":23,"journal-issue":{"issue":"149","published-online":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.3791\/59452","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,10]]}}}