{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:39:40Z","timestamp":1792633180161,"version":"4.5.0"},"reference-count":32,"publisher":"MyJove Corporation","issue":"148","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/59783","type":"journal-article","created":{"date-parts":[[2019,6,6]],"date-time":"2019-06-06T20:00:16Z","timestamp":1559851216000},"source":"Crossref","is-referenced-by-count":3,"title":["Measuring Sperm Guidance and Motility within the <em>Caenorhabditis elegans<\/em> Hermaphrodite Reproductive Tract"],"prefix":"10.3791","author":[{"given":"Muhan","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shara","family":"Legg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael  A.","family":"Miller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2019,6,6]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/humrep\/dev002","type":"journal-article","article-title":"Behavioral mechanism of human sperm in thermotaxis: a role for hyperactivation","volume":"30","author":"Boryshpolets","year":"2015","unstructured":"Boryshpolets, S., Perez-Cerezales, S., & Eisenbach, M. Behavioral mechanism of human sperm in thermotaxis: a role for hyperactivation. Human Reproduction. 30 (4), 884-892, (2015).","journal-title":"Human Reproduction"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.ydbio.2011.08.014","type":"journal-article","article-title":"The gap junctional protein INX-14 functions in oocyte precursors to promote C. elegans sperm guidance","volume":"359","author":"Edmonds","year":"2011","unstructured":"Edmonds, J. W., McKinney, S. L., Prasain, J. K., & Miller, M. A. The gap junctional protein INX-14 functions in oocyte precursors to promote C. elegans sperm guidance. Developmental Biology. 359 (1), 47-58, (2011).","journal-title":"Developmental Biology"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.devcel.2010.11.005","type":"journal-article","article-title":"Insulin\/FOXO signaling regulates ovarian prostaglandins critical for reproduction","volume":"19","author":"Edmonds","year":"2010","unstructured":"Edmonds, J. W. et al. Insulin\/FOXO signaling regulates ovarian prostaglandins critical for reproduction. Developmental Cell. 19 (6), 858-871, (2010).","journal-title":"Developmental Cell"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"4236","DOI":"10.1038\/s41598-017-03857-9","type":"journal-article","article-title":"Network model predicts that CatSper is the main Ca(2+) channel in the regulation of sea urchin sperm motility","volume":"7","author":"Espinal-Enriquez","year":"2017","unstructured":"Espinal-Enriquez, J., Priego-Espinosa, D. A., Darszon, A., Beltran, C., & Martinez-Mekler, G. Network model predicts that CatSper is the main Ca(2+) channel in the regulation of sea urchin sperm motility. Scientific Reports. 7 (1), 4236, (2017).","journal-title":"Scientific Reports"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1530\/jrf.0.0770599","type":"journal-article","article-title":"A preovulatory temperature gradient between the isthmus and ampulla of pig oviducts during the phase of sperm storage","volume":"77","author":"Hunter","year":"1986","unstructured":"Hunter, R. H., & Nichol, R. A preovulatory temperature gradient between the isthmus and ampulla of pig oviducts during the phase of sperm storage. Journal of Reproduction and Fertility. 77 (2), 599-606, (1986).","journal-title":"Journal of Reproduction and Fertility"},{"issue":"Pt 10","key":"ref6","first-page":"1458","type":"journal-article","article-title":"Sperm chemotaxis promotes individual fertilization success in sea urchins","volume":"219","author":"Hussain","year":"2016","unstructured":"Hussain, Y. H., Guasto, J. S., Zimmer, R. K., Stocker, R., & Riffell, J. A. Sperm chemotaxis promotes individual fertilization success in sea urchins. Journal of Experimental Biology. 219 (Pt 10), 1458-1466, (2016).","journal-title":"Journal of Experimental Biology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e02403","DOI":"10.7554\/eLife.02403","type":"journal-article","article-title":"Rheotaxis facilitates upstream navigation of mammalian sperm cells","volume":"3","author":"Kantsler","year":"2014","unstructured":"Kantsler, V., Dunkel, J., Blayney, M., & Goldstein, R. E. Rheotaxis facilitates upstream navigation of mammalian sperm cells. Elife. 3 e02403, (2014).","journal-title":"Elife"},{"issue":"10","key":"ref8","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/ncb1476","type":"journal-article","article-title":"Oocyte signals derived from polyunsaturated fatty acids control sperm recruitment in vivo","volume":"8","author":"Kubagawa","year":"2006","unstructured":"Kubagawa, H. M. et al. Oocyte signals derived from polyunsaturated fatty acids control sperm recruitment in vivo. Nature Cell Biology. 8 (10), 1143-1148, (2006).","journal-title":"Nature Cell Biology"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.cub.2013.02.007","type":"journal-article","article-title":"Rheotaxis guides mammalian sperm","volume":"23","author":"Miki","year":"2013","unstructured":"Miki, K., & Clapham, D. E. Rheotaxis guides mammalian sperm. Current Biology. 23 (6), 443-452, (2013).","journal-title":"Current Biology"},{"issue":"12","key":"ref10","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1534\/g3.118.200785","type":"journal-article","article-title":"Genetic Contributions to Ectopic Sperm Cell Migration in Caenorhabditis Nematodes","volume":"8","author":"Ting","year":"2018","unstructured":"Ting, J. J., Tsai, C. N., Schalkowski, R., & Cutter, A. D. Genetic Contributions to Ectopic Sperm Cell Migration in Caenorhabditis Nematodes. G3 (Bethesda). 8 (12), 3891-3902, (2018).","journal-title":"G3"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1093\/biolre\/iox015","type":"journal-article","article-title":"Chemical and physical guidance of fish spermatozoa into the egg through the micropyledagger, double dagger","volume":"96","author":"Yanagimachi","year":"2017","unstructured":"Yanagimachi, R. et al. Chemical and physical guidance of fish spermatozoa into the egg through the micropyledagger,double dagger. Biology of Reproduction. 96 (4), 780-799, (2017).","journal-title":"Biology of Reproduction"},{"issue":"11","key":"ref12","doi-asserted-by":"crossref","first-page":"e0142555","DOI":"10.1371\/journal.pone.0142555","type":"journal-article","article-title":"Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis","volume":"10","author":"Zhang","year":"2015","unstructured":"Zhang, Y. et al. Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis. PloS One. 10 (11), e0142555, (2015).","journal-title":"PloS One"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"e2002047","DOI":"10.1371\/journal.pbio.2002047","type":"journal-article","article-title":"Chemosensory and hyperoxia circuits in C. elegans males influence sperm navigational capacity","volume":"15","author":"Hoang","year":"2017","unstructured":"Hoang, H. D., & Miller, M. A. Chemosensory and hyperoxia circuits in C. elegans males influence sperm navigational capacity. PLoS Biology. 15 (6), e2002047, (2017).","journal-title":"PLoS Biology"},{"key":"ref14","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.05423","type":"journal-article","article-title":"COMP-1 promotes competitive advantage of nematode sperm","volume":"4","author":"Hansen","year":"2015","unstructured":"Hansen, J. M., Chavez, D. R., & Stanfield, G. M. COMP-1 promotes competitive advantage of nematode sperm. Elife. 4, (2015).","journal-title":"Elife"},{"key":"ref15","first-page":"1","type":"journal-article","article-title":"Spermatogenesis","author":"L'Hernault","year":"2006","unstructured":"L'Hernault, S. W. Spermatogenesis. WormBook: The Online Review of C. Elegans Biology. 1-14, (2006).","journal-title":"WormBook: The Online Review of C. Elegans Biology"},{"issue":"5511","key":"ref16","doi-asserted-by":"crossref","first-page":"2144","DOI":"10.1126\/science.1057586","type":"journal-article","article-title":"A sperm cytoskeletal protein that signals oocyte meiotic maturation and ovulation","volume":"291","author":"Miller","year":"2001","unstructured":"Miller, M. A. et al. A sperm cytoskeletal protein that signals oocyte meiotic maturation and ovulation. Science. 291 (5511), 2144-2147, (2001).","journal-title":"Science"},{"key":"ref17","first-page":"1","type":"journal-article","article-title":"Control of oocyte meiotic maturation and fertilization","author":"Greenstein","year":"2005","unstructured":"Greenstein, D. Control of oocyte meiotic maturation and fertilization. WormBook: The Online Review of C. Elegans Biology. 1-12, (2005).","journal-title":"WormBook: The Online Review of C. Elegans Biology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.semcdb.2014.06.001","type":"journal-article","article-title":"Mating behavior, male sensory cilia, and polycystins in Caenorhabditis elegans","volume":"33","author":"O'Hagan","year":"2014","unstructured":"O'Hagan, R., Wang, J., & Barr, M. M. Mating behavior, male sensory cilia, and polycystins in Caenorhabditis elegans. Seminars in Cell & Developmental Biology. 33 25-33, (2014).","journal-title":"Seminars in Cell & Developmental Biology"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"e1003271","DOI":"10.1371\/journal.pgen.1003271","type":"journal-article","article-title":"A heterogeneous mixture of F-series prostaglandins promotes sperm guidance in the Caenorhabditis elegans reproductive tract","volume":"9","author":"Hoang","year":"2013","unstructured":"Hoang, H. D., Prasain, J. K., Dorand, D., & Miller, M. A. A heterogeneous mixture of F-series prostaglandins promotes sperm guidance in the Caenorhabditis elegans reproductive tract. PLoS Genetics. 9 (1), e1003271, (2013).","journal-title":"PLoS Genetics"},{"issue":"6185","key":"ref20","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1126\/science.1250598","type":"journal-article","article-title":"Neurosensory perception of environmental cues modulates sperm motility critical for fertilization","volume":"344","author":"McKnight","year":"2014","unstructured":"McKnight, K. et al. Neurosensory perception of environmental cues modulates sperm motility critical for fertilization. Science. 344 (6185), 754-757, (2014).","journal-title":"Science"},{"issue":"47","key":"ref21","doi-asserted-by":"crossref","DOI":"10.3791\/2293","type":"journal-article","article-title":"An introduction to worm lab: from culturing worms to mutagenesis","author":"Chaudhuri","year":"2011","unstructured":"Chaudhuri, J., Parihar, M., & Pires-daSilva, A. An introduction to worm lab: from culturing worms to mutagenesis. Journal of Visualized Experiments. (47), (2011).","journal-title":"Journal of Visualized Experiments"},{"key":"ref22","first-page":"1","type":"journal-article","article-title":"Maintenance of C. elegans","author":"Stiernagle","year":"2006","unstructured":"Stiernagle, T. Maintenance of C. elegans. WormBook: The Online Review of C. Elegans Biology. 1-11, (2006).","journal-title":"WormBook: The Online Review of C. Elegans Biology"},{"issue":"11","key":"ref23","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1016\/j.exger.2013.07.014","type":"journal-article","article-title":"Dramatic fertility decline in aging C. elegans males is associated with mating execution deficits rather than diminished sperm quality","volume":"48","author":"Chatterjee","year":"2013","unstructured":"Chatterjee, I. et al. Dramatic fertility decline in aging C. elegans males is associated with mating execution deficits rather than diminished sperm quality. Experimental Gerontology. 48 (11), 1156-1166, (2013).","journal-title":"Experimental Gerontology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.ymeth.2016.09.016","type":"journal-article","article-title":"TrackMate: An open and extensible platform for single-particle tracking","author":"Tinevez","year":"2017","unstructured":"Tinevez, J. Y. et al. TrackMate: An open and extensible platform for single-particle tracking. Methods. 115 80-90, (2017).","journal-title":"Methods"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1534\/genetics.115.176099","type":"journal-article","article-title":"A Transparent Window into Biology: A Primer on Caenorhabditis elegans","volume":"200","author":"Corsi","year":"2015","unstructured":"Corsi, A. K., Wightman, B., & Chalfie, M. A Transparent Window into Biology: A Primer on Caenorhabditis elegans. Genetics. 200 (2), 387-407, (2015).","journal-title":"Genetics"},{"issue":"6059","key":"ref26","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1126\/science.1210333","type":"journal-article","article-title":"Degradation of paternal mitochondria by fertilization-triggered autophagy in C. elegans embryos","volume":"334","author":"Sato","year":"2011","unstructured":"Sato, M., & Sato, K. Degradation of paternal mitochondria by fertilization-triggered autophagy in C. elegans embryos. Science. 334 (6059), 1141-1144, (2011).","journal-title":"Science"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"12569","DOI":"10.1038\/ncomms12569","type":"journal-article","article-title":"Kinetics and specificity of paternal mitochondrial elimination in Caenorhabditis elegans","volume":"7","author":"Wang","year":"2016","unstructured":"Wang, Y. et al. Kinetics and specificity of paternal mitochondrial elimination in Caenorhabditis elegans. Nature Communications. 7 12569, (2016).","journal-title":"Nature Communications"},{"issue":"12","key":"ref28","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1242\/dev.004952","type":"journal-article","article-title":"Actin-dependent cytoplasmic streaming in C. elegans oogenesis","volume":"134","author":"Wolke","year":"2007","unstructured":"Wolke, U., Jezuit, E. A., & Priess, J. R. Actin-dependent cytoplasmic streaming in C. elegans oogenesis. Development. 134 (12), 2227-2236, (2007).","journal-title":"Development"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.3762\/bjoc.8.243","type":"journal-article","article-title":"Hydrophobic analogues of rhodamine B and rhodamine 101: potent fluorescent probes of mitochondria in living C. elegans","volume":"8","author":"Mottram","year":"2012","unstructured":"Mottram, L. F., Forbes, S., Ackley, B. D., & Peterson, B. R. Hydrophobic analogues of rhodamine B and rhodamine 101: potent fluorescent probes of mitochondria in living C. elegans. Beilstein Journal of Organic Chemistry. 8 2156-2165, (2012).","journal-title":"Beilstein Journal of Organic Chemistry"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1093\/genetics\/152.1.201","type":"journal-article","article-title":"Sperm competition in the absence of fertilization in Caenorhabditis elegans","volume":"152","author":"Singson","year":"1999","unstructured":"Singson, A., Hill, K. L., & L'Hernault, S. W. Sperm competition in the absence of fertilization in Caenorhabditis elegans. Genetics. 152 (1), 201-208, (1999).","journal-title":"Genetics"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1534\/genetics.111.135376","type":"journal-article","article-title":"Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans","volume":"190","author":"Wu","year":"2012","unstructured":"Wu, J. C. et al. Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans. Genetics. 190 (1), 143-157, (2012).","journal-title":"Genetics"},{"issue":"7","key":"ref32","doi-asserted-by":"crossref","first-page":"e1001115","DOI":"10.1371\/journal.pbio.1001115","type":"journal-article","article-title":"A novel sperm-delivered toxin causes late-stage embryo lethality and transmission ratio distortion in C. elegans","volume":"9","author":"Seidel","year":"2011","unstructured":"Seidel, H. S. et al. A novel sperm-delivered toxin causes late-stage embryo lethality and transmission ratio distortion in C. elegans. PLoS Biology. 9 (7), e1001115, (2011).","journal-title":"PLoS Biology"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/59783\/measuring-sperm-guidance-motility-within-caenorhabditis-elegans","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:55:19Z","timestamp":1792630519000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/59783"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,6]]},"references-count":32,"journal-issue":{"issue":"148","published-online":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.3791\/59783","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,6]]}}}