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Journal of Medical Microbiology. 51 (9), 717-722 (2002).","journal-title":"Journal of Medical Microbiology"},{"issue":"38","key":"ref28","doi-asserted-by":"crossref","first-page":"4962","DOI":"10.1016\/j.vaccine.2008.06.093","type":"journal-article","article-title":"Induction of opsonophagocytic killing activity with pneumococcal conjugate vaccine in human immunodeficiency virus-infected Ugandan adults","volume":"26","author":"Chen","year":"2008","unstructured":"Chen, M. et al. Induction of opsonophagocytic killing activity with pneumococcal conjugate vaccine in human immunodeficiency virus-infected Ugandan adults. Vaccine. 26 (38), 4962-4968 (2008).","journal-title":"Vaccine"},{"issue":"146","key":"ref29","first-page":"e59400","type":"journal-article","article-title":"Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens","author":"Paschall","year":"2019","unstructured":"Paschall, A. V., Middleton, D. R., Avci, F. Y. Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens. Journal of Visualized Experiments. (146) 59400 (2019).","journal-title":"Journal of Visualized Experiments"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1046\/j.1365-2672.1999.00881.x","type":"journal-article","article-title":"The capsule of Bacillus anthracis, a review","volume":"87","author":"Ezzell","year":"1999","unstructured":"Ezzell, J. W., Welkos, S. L. The capsule of Bacillus anthracis, a review. Journal of Applied Microbiology. 87 (2), 250 (1999).","journal-title":"Journal of Applied Microbiology"},{"issue":"21","key":"ref31","first-page":"10198","type":"journal-article","article-title":"On the role of macrophages in anthrax","volume":"90","author":"Hanna","year":"1993","unstructured":"Hanna, P. C., Acosta, D., Collier, R. J. On the role of macrophages in anthrax. Proceedings of the National Academies of Sciences of the United States of America. 90 (21), 10198-10201 (1993).","journal-title":"Proceedings of the National Academies of Sciences of the United States of America"},{"issue":"1","key":"ref32","first-page":"19","type":"journal-article","article-title":"Use of HL-60 cell line to measure opsonic capacity of pneumococcal antibodies","volume":"12","author":"Fleck","year":"2005","unstructured":"Fleck, R. A., Romero-Steiner, S., Nahm, M. H. Use of HL-60 cell line to measure opsonic capacity of pneumococcal antibodies. Clinical and Diagnostic Laboratory Immunology. 12 (1), 19-27 (2005).","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"issue":"35","key":"ref33","doi-asserted-by":"crossref","first-page":"36982","DOI":"10.1074\/jbc.M405082200","type":"journal-article","article-title":"Mycobacterium tuberculosis reprograms waves of phosphatidylinositol 3-phosphate on phagosomal organelles","volume":"279","author":"Chua","year":"2004","unstructured":"Chua, J., Deretic, V. Mycobacterium tuberculosis reprograms waves of phosphatidylinositol 3-phosphate on phagosomal organelles. Journal of Biological Chemistry. 279 (35), 36982-36992 (2004).","journal-title":"Journal of Biological Chemistry"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00586-16","type":"journal-article","article-title":"pH Alkalinization by Chloroquine Suppresses Pathogenic Burkholderia Type 6 Secretion System 1 and Multinucleated Giant Cells","volume":"85","author":"Chua","year":"2017","unstructured":"Chua, J. et al. pH Alkalinization by Chloroquine Suppresses Pathogenic Burkholderia Type 6 Secretion System 1 and Multinucleated Giant Cells. Infection & Immunity. 85 (1), (2017).","journal-title":"Infection & Immunity"},{"issue":"12","key":"ref35","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1093\/intimm\/13.12.1551","type":"journal-article","article-title":"Differences in promiscuity for antibody-FcRn interactions across species: implications for therapeutic antibodies","volume":"13","author":"Ober","year":"2001","unstructured":"Ober, R. 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