{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:40:30Z","timestamp":1792626030259,"version":"4.5.0"},"reference-count":32,"publisher":"MyJove Corporation","issue":"159","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61297","type":"journal-article","created":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T09:38:30Z","timestamp":1590140310000},"source":"Crossref","is-referenced-by-count":2,"title":["Imaging Intranuclear Actin Rods in Live Heat Stressed &lt;em&gt;Drosophila&lt;\/em&gt; Embryos"],"prefix":"10.3791","author":[{"given":"Natalie","family":"Biel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Figard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Marie","family":"Sokac","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"issue":"49","key":"ref1","doi-asserted-by":"crossref","first-page":"3493","DOI":"10.1016\/j.celrep.2019.02.092","type":"journal-article","article-title":"Cofilin-mediated Actin Stress Response is maladaptive in heat-stressed embryos","volume":"26","author":"Figard","year":"2019","unstructured":"Figard, L. et al. Cofilin-mediated Actin Stress Response is maladaptive in heat-stressed embryos. Cell Reports. 26 (49), 3493-3501 (2019).","journal-title":"Cell Reports"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1152\/ajprenal.00210.2002","type":"journal-article","article-title":"ADF\/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion","volume":"284","author":"Ashworth","year":"2003","unstructured":"Ashworth, S. L. et al. ADF\/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion. American Journal of Physiology Renal Physiology. 284 (4), F852-62 (2003).","journal-title":"American Journal of Physiology Renal Physiology"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1097\/NEN.0b013e3181d892c6","type":"journal-article","article-title":"In vitro analysis of rod composition and actin dynamics in inherited myopathies","volume":"69","author":"Vandebrouck","year":"2010","unstructured":"Vandebrouck, A. et al. In vitro analysis of rod composition and actin dynamics in inherited myopathies. Journal of Neuropathology and Experimental Neurology. 69 (5), 429-441 (2010).","journal-title":"Journal of Neuropathology and Experimental Neurology"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1038\/35023579","type":"journal-article","article-title":"Neurodegenerative stimuli induce persistent ADF\/cofilin-actin rods that disrupt distal neurite function","volume":"2","author":"Minamide","year":"2000","unstructured":"Minamide, L. S., Striegl, A. M., Boyle, J. A., Meberg, P. J., Bamburg, J. R. Neurodegenerative stimuli induce persistent ADF\/cofilin-actin rods that disrupt distal neurite function. Nature Cell Biology. 2 (9), 628-636 (2000).","journal-title":"Nature Cell Biology"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"241","DOI":"10.2174\/156720510791050902","type":"journal-article","article-title":"ADF\/Cofilin-actin rods in neurodegenerative diseases","volume":"7","author":"Bamburg","year":"2010","unstructured":"Bamburg, J. R. et al. ADF\/Cofilin-actin rods in neurodegenerative diseases. Current Alzheimer Research. 7 (3), 241-50 (2010).","journal-title":"Current Alzheimer Research"},{"issue":"9","key":"ref6","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1002\/cm.21282","type":"journal-article","article-title":"Actin dynamics and cofilin-actin rods in alzheimer disease","volume":"73","author":"Bamburg","year":"2016","unstructured":"Bamburg, J. R., Bernstein, B. W. Actin dynamics and cofilin-actin rods in alzheimer disease. Cytoskeleton. 73 (9), 477-497 (2016).","journal-title":"Cytoskeleton"},{"issue":"5","key":"ref7","first-page":"828","type":"journal-article","article-title":"Formation of actin-ADF\/cofilin rods transiently retards decline of mitochondrial potential and ATP in stressed neurons","volume":"291","author":"Bernstein","year":"2006","unstructured":"Bernstein, B. W., Chen, H., Boyle, J. A., Bamburg, J. R. Formation of actin-ADF\/cofilin rods transiently retards decline of mitochondrial potential and ATP in stressed neurons. AJP: Cell Physiology. 291 (5), C828-C839 (2006).","journal-title":"AJP: Cell Physiology"},{"issue":"17","key":"ref8","first-page":"3977","type":"journal-article","article-title":"Cofilin nuclear-cytoplasmic shuttling affects cofilin-actin rod formation during stress","volume":"125","author":"Munsie","year":"2012","unstructured":"Munsie, L. N., Desmond, C. R., Truant, R. Cofilin nuclear-cytoplasmic shuttling affects cofilin-actin rod formation during stress. Journal of Cell Science. 125 (17), 3977-3988 (2012).","journal-title":"Journal of Cell Science"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0014-4827(86)90545-8","type":"journal-article","article-title":"Heat shock induction of intranuclear actin rods in cultured mammalian cells","volume":"165","author":"Iida","year":"1986","unstructured":"Iida, K., Iida, H., Yahara, I. Heat shock induction of intranuclear actin rods in cultured mammalian cells. Experimental Cell Research. 165, 207-215 (1986).","journal-title":"Experimental Cell Research"},{"issue":"27","key":"ref10","doi-asserted-by":"crossref","first-page":"16143","DOI":"10.1016\/S0021-9258(18)71598-6","type":"journal-article","article-title":"Dephosphorylation of cofilin accompanies heat shock-induced nuclear accumulation of cofilin","volume":"264","author":"Ohta","year":"1989","unstructured":"Ohta, Y., Nishida, E., Sakai, H., Miyamoto, E. Dephosphorylation of cofilin accompanies heat shock-induced nuclear accumulation of cofilin. Journal of Biological Chemistry. 264 (27), 16143-16148 (1989).","journal-title":"Journal of Biological Chemistry"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1247\/csf.17.39","type":"journal-article","article-title":"The KKRKK sequence is involved in heat shock-induced nuclear translocation of the 18-kDa actin-binding protein, cofilin","volume":"17","author":"Iida","year":"1992","unstructured":"Iida, K., Matsumoto, S., Yahara, I. The KKRKK sequence is involved in heat shock-induced nuclear translocation of the 18-kDa actin-binding protein, cofilin. Cell Structure and Function. 17 (1), 39-46 (1992).","journal-title":"Cell Structure and Function"},{"issue":"8","key":"ref12","doi-asserted-by":"crossref","first-page":"5450","DOI":"10.1074\/jbc.M109.063768","type":"journal-article","article-title":"Isolation and characterization of cytoplasmic cofilin-actin rods","volume":"285","author":"Minamide","year":"2010","unstructured":"Minamide, L. S. et al. Isolation and characterization of cytoplasmic cofilin-actin rods. Journal of Biological Chemistry. 285 (8), 5450-5460 (2010).","journal-title":"Journal of Biological Chemistry"},{"issue":"7","key":"ref13","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1083\/jcb.44.1.172","type":"journal-article","article-title":"Origin, development, and nature of intranuclear rodlets and associated bodies in chicken sympathetic neurons","volume":"44","author":"Masurovsky","year":"1970","unstructured":"Masurovsky, E. B., Benitez, H. H., Kim, S. U., Murray, M. R. Origin, development, and nature of intranuclear rodlets and associated bodies in chicken sympathetic neurons. The Journal of Cell Biology. 44 (7), 172-191 (1970).","journal-title":"The Journal of Cell Biology"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/BF01099179","type":"journal-article","article-title":"Intranuclear rods and sheets in rat cochlear nucleus","volume":"1","author":"Feldman","year":"1972","unstructured":"Feldman, M. L., Peters, A. Intranuclear rods and sheets in rat cochlear nucleus. Journal of Neurocytology. 1 (2), 109-127 (1972).","journal-title":"Journal of Neurocytology"},{"issue":"8","key":"ref15","first-page":"5262","type":"journal-article","article-title":"Cofilin is a component of intranuclear and cytoplasmic actin rods induced in cultured cells","volume":"84","author":"Nishida","year":"1987","unstructured":"Nishida, E. et al. Cofilin is a component of intranuclear and cytoplasmic actin rods induced in cultured cells. Cell Biology. 84 (8), 5262-5266 (1987).","journal-title":"Cell Biology"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/BF00115454","type":"journal-article","article-title":"Colocalization of ADF and cofilin in intranuclear actin rods of cultured muscle cells","volume":"14","author":"Ono","year":"1993","unstructured":"Ono, S., Abe, H., Nagaoka, R., Obinata, T. Colocalization of ADF and cofilin in intranuclear actin rods of cultured muscle cells. Journal of Muscle Research and Cell Motility. 14 (2), 195-204 (1993).","journal-title":"Journal of Muscle Research and Cell Motility"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1083\/jcb.201608025","type":"journal-article","article-title":"Back-to-back mechanisms drive actomyosin ring closure during Drosophila embryo cleavage","volume":"215","author":"Xue","year":"2016","unstructured":"Xue, Z., Sokac, A. M. Back-to-back mechanisms drive actomyosin ring closure during Drosophila embryo cleavage. The Journal of Cell Biology. 215 (3), 335-344 (2016).","journal-title":"The Journal of Cell Biology"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1083\/jcb.200712036","type":"journal-article","article-title":"Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization","volume":"182","author":"Cao","year":"2008","unstructured":"Cao, J., Albertson, R., Riggs, B., Field, C. M., Sullivan, W. Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization. Journal of Cell Biology. 182 (2), 301-313 (2008).","journal-title":"Journal of Cell Biology"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.ydbio.2014.06.022","type":"journal-article","article-title":"The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis","volume":"393","author":"Spracklen","year":"2014","unstructured":"Spracklen, A. J., Fagan, T. N., Lovander, K. E., Tootle, T. L. The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis. Developmental Biology. 393 (2), 209-226 (2014).","journal-title":"Developmental Biology"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jsb.2011.10.005","type":"journal-article","article-title":"Formins filter modified actin subunits during processive elongation","volume":"177","author":"Chen","year":"2012","unstructured":"Chen, Q., Nag, S., Pollard, T. D. Formins filter modified actin subunits during processive elongation. Journal of Structural Biology. 177 (1), 32-39 (2012).","journal-title":"Journal of Structural Biology"},{"issue":"50","key":"ref21","first-page":"2477","type":"journal-article","article-title":"RNAi interference by dsRNA injection into Drosophila embryos","author":"Iordanou","year":"2011","unstructured":"Iordanou, E., Chandran, R. R., Blackstone, N., Jiang, L. RNAi interference by dsRNA injection into Drosophila embryos. Journal of Visualized Experiments. 50, 2477 (2011).","journal-title":"Journal of Visualized Experiments"},{"key":"ref22","first-page":"50750","type":"journal-article","article-title":"Microinjection wound assay and in vivo localization of epidermal wound response reporters in Drosophila embryos","volume":"81","author":"Juarez","year":"2013","unstructured":"Juarez, M. T., Patterson, R. A., Li, W., McGinnis, W. Microinjection wound assay and in vivo localization of epidermal wound response reporters in Drosophila embryos. Journal of Visualized Experiments. 81, 50750 (2013).","journal-title":"Journal of Visualized Experiments"},{"issue":"77","key":"ref23","first-page":"50346","type":"journal-article","article-title":"Recombineering homologous recombination constructs in Drosophila","author":"Carreira-Rosario","year":"2013","unstructured":"Carreira-Rosario, A. et al. Recombineering homologous recombination constructs in Drosophila. Journal of Visualized Experiments. 77, 50346 (2013).","journal-title":"Journal of Visualized Experiments"},{"issue":"31","key":"ref24","first-page":"1382","type":"journal-article","article-title":"Microinjection techniques for studying mitosis in the Drosophila melanogaster syncytial embryo","author":"Brust-Mascher","year":"2009","unstructured":"Brust-Mascher, I., Scholey, J. M. Microinjection techniques for studying mitosis in the Drosophila melanogaster syncytial embryo. Journal of Visualized Experiments. 31, 1382 (2009).","journal-title":"Journal of Visualized Experiments"},{"issue":"148","key":"ref25","first-page":"58545","type":"journal-article","article-title":"Visualizing and tracking endogenous mRNAs in live Drosophila melanogaster egg chambers","author":"Catrina","year":"2019","unstructured":"Catrina, I. E., Bayer, L. V., Omar, O. S., Bratu, D. P. Visualizing and tracking endogenous mRNAs in live Drosophila melanogaster egg chambers. Journal of Visualized Experiments. 148, 58545 (2019).","journal-title":"Journal of Visualized Experiments"},{"issue":"8","key":"ref26","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1016\/j.bpj.2015.02.032","type":"journal-article","article-title":"The mechanical properties of early Drosophila embryos measured by high-speed video microrheology","volume":"108","author":"Wessel","year":"2015","unstructured":"Wessel, A. D., Gumalla, M., Grosshans, J., Schmidt, C. F. The mechanical properties of early Drosophila embryos measured by high-speed video microrheology. Biophysical Journal. 108 (8), 1899-1907 (2015).","journal-title":"Biophysical Journal"},{"key":"ref27","type":"book","article-title":"Microinjection and transgenesis","volume-title":"Microinjection and Transgenesis: Strategies and Protocols","author":"Mollinari","year":"1998","unstructured":"Mollinari, C., Gonz\u00e1lez. Microinjection and transgenesis. Microinjection and Transgenesis: Strategies and Protocols. Edited by Cid-Arregui, A., Garc\u00eda-Carranc\u00e1, A., 587-603, Springer. Berlin (1998)."},{"issue":"49","key":"ref28","first-page":"2503","type":"journal-article","article-title":"Imaging cell shape change in living Drosophila embryos","author":"Figard","year":"2011","unstructured":"Figard, L., Sokac, A. M. Imaging cell shape change in living Drosophila embryos. Journal of Visualized Experiments. 49, 2503 (2011).","journal-title":"Journal of Visualized Experiments"},{"key":"ref29","type":"book","volume-title":"Pipette Cookbook 2018: P-97 and P-1000 Micropipette Pullers","author":"Oesterle","year":"2018","unstructured":"Oesterle, A. Pipette Cookbook 2018: P-97 and P-1000 Micropipette Pullers. Sutter Instruments. Novato, CA (2018)."},{"issue":"3","key":"ref30","first-page":"789","type":"journal-article","article-title":"A photographic study of development in the living embryo of Drosophila melanogaster","volume":"33","author":"Bownes","year":"1975","unstructured":"Bownes, M. A photographic study of development in the living embryo of Drosophila melanogaster. Journal of Embryology and Experimental Morphology. 33 (3), 789-801 (1975).","journal-title":"Journal of Embryology and Experimental Morphology"},{"issue":"4","key":"ref31","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1086\/physzool.8.4.30151263","type":"journal-article","article-title":"The effects of temperature on the durations of the developmental stages of Drosophila melanogaster","volume":"8","author":"Powsner","year":"1935","unstructured":"Powsner, L. The effects of temperature on the durations of the developmental stages of Drosophila melanogaster. Physiological Zoology. 8 (4), 474-520 (1935).","journal-title":"Physiological Zoology"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.devcel.2018.10.013","type":"journal-article","article-title":"Oxidative Stress Orchestrates Cell Polarity to Promote Embryonic Wound Healing","volume":"47","author":"Hunter","year":"2018","unstructured":"Hunter, M. V., Willoughby, P. M., Bruce, A. E. E., Fernandez-Gonzalez, R. Oxidative Stress Orchestrates Cell Polarity to Promote Embryonic Wound Healing. Developmental Cell. 47 (3), 377-387 (2018).","journal-title":"Developmental Cell"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61297\/imaging-intranuclear-actin-rods-live-heat-stressed-drosophila","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:11:27Z","timestamp":1792624287000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,15]]},"references-count":32,"journal-issue":{"issue":"159","published-online":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.3791\/61297","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,15]]}}}