{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:38:37Z","timestamp":1792625917209,"version":"4.5.0"},"reference-count":20,"publisher":"MyJove Corporation","issue":"160","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61365","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T20:01:06Z","timestamp":1591819266000},"source":"Crossref","is-referenced-by-count":7,"title":["Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in <em>Chlamydia trachomatis<\/em>"],"prefix":"10.3791","author":[{"given":"Travis  J.","family":"Chiarelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole  A.","family":"Grieshaber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott S.","family":"Grieshaber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2020,6,10]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.femsre.2005.03.002","type":"journal-article","article-title":"The chlamydial developmental cycle","volume":"29","author":"AbdelRahman","year":"2005","unstructured":"AbdelRahman, Y., Belland, R. The chlamydial developmental cycle. FEMS Microbiology Reviews. 29 (5), 949\u2013959 (2005).","journal-title":"FEMS Microbiology Reviews"},{"issue":"27","key":"ref2","doi-asserted-by":"crossref","first-page":"10166","DOI":"10.1073\/pnas.0402829101","type":"journal-article","article-title":"A chlamydial type III translocated protein is tyrosine-phosphorylated at the site of entry and associated with recruitment of actin","volume":"101","author":"Clifton","year":"2004","unstructured":"Clifton, D. et al. A chlamydial type III translocated protein is tyrosine-phosphorylated at the site of entry and associated with recruitment of actin. Proceedings of the National Academy of Sciences U. S. A. 101 (27), 10166-10171 (2004).","journal-title":"Proceedings of the National Academy of Sciences U. S. A"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1046\/j.1365-2958.2003.03708.x","type":"journal-article","article-title":"\u03c328 RNA polymerase regulates hctB, a late developmental gene in Chlamydia","volume":"50","author":"Yu","year":"2003","unstructured":"Yu, H. H. Y., Tan, M. \u03c328 RNA polymerase regulates hctB, a late developmental gene in Chlamydia. Molecular Microbiology. 50 (2), 577-584 (2003).","journal-title":"Molecular Microbiology"},{"issue":"19","key":"ref4","doi-asserted-by":"crossref","first-page":"17314","DOI":"10.1074\/jbc.M212170200","type":"journal-article","article-title":"A Developmentally Regulated Two-component Signal Transduction System in Chlamydia","volume":"278","author":"Koo","year":"2003","unstructured":"Koo, I., Stephens, R. A Developmentally Regulated Two-component Signal Transduction System in Chlamydia. Journal of Biological Chemistry. 278 (19), 17314\u201317319 (2003).","journal-title":"Journal of Biological Chemistry"},{"issue":"14","key":"ref5","doi-asserted-by":"crossref","first-page":"00065","DOI":"10.1128\/JB.00065-18","type":"journal-article","article-title":"Impact of Active Metabolism on Elementary Body Transcript Profile and Infectivity","volume":"200","author":"Grieshaber","year":"2018","unstructured":"Grieshaber, S. et al. Impact of Active Metabolism on Elementary Body Transcript Profile and Infectivity. Journal of Bacteriology. 200 (14), e00065-18 (2018).","journal-title":"Journal of Bacteriology"},{"issue":"14","key":"ref6","doi-asserted-by":"crossref","first-page":"8478","DOI":"10.1073\/pnas.1331135100","type":"journal-article","article-title":"Genomic transcriptional profiling of the developmental cycle of","volume":"100","author":"Belland","year":"2003","unstructured":"Belland, R., et al. Genomic transcriptional profiling of the developmental cycle of. Proceedings of the National Academy of Sciences U. S. A. 100 (14), 8478-8483 (2003).","journal-title":"Proceedings of the National Academy of Sciences U. S. A"},{"issue":"9","key":"ref7","doi-asserted-by":"crossref","first-page":"1002258","DOI":"10.1371\/journal.ppat.1002258","type":"journal-article","article-title":"Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector","volume":"7","author":"Wang","year":"2011","unstructured":"Wang, Y., et al. Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector. PLoS Pathogens. 7 (9), e1002258. (2011).","journal-title":"PLoS Pathogens"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1073\/pnas.1117884109","type":"journal-article","article-title":"Virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis revealed by forward genetic approaches","volume":"109","author":"Nguyen","year":"2012","unstructured":"Nguyen, B., Valdivia, R. Virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis revealed by forward genetic approaches. Proceedings of the National Academy of Sciences U. S. A. 109 (4), 1263-1268 (2012).","journal-title":"Proceedings of the National Academy of Sciences U. S. A"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"01817","DOI":"10.1128\/mBio.01817-15","type":"journal-article","article-title":"Gene Deletion by Fluorescence-Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis","volume":"7","author":"Mueller","year":"2016","unstructured":"Mueller, K., Wolf, K., Fields, K., Maurelli, A. Gene Deletion by Fluorescence-Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis. MBio. 7 (1), e01817-15 (2016).","journal-title":"MBio"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1111\/j.1365-2958.2012.08077.x","type":"journal-article","article-title":"The early gene product EUO is a transcriptional repressor that selectively regulates promoters of Chlamydia late genes","volume":"84","author":"Rosario","year":"2012","unstructured":"Rosario, C. J., Tan, M. The early gene product EUO is a transcriptional repressor that selectively regulates promoters of Chlamydia late genes. Mol Microbiol. 84 (6), 1097\u20131107 (2012).","journal-title":"Mol Microbiol"},{"issue":"14","key":"ref11","doi-asserted-by":"crossref","first-page":"4274","DOI":"10.1128\/jb.175.14.4274-4281.1993","type":"journal-article","article-title":"Molecular cloning and expression of hctB encoding a strain-variant chlamydial histone-like protein with DNA-binding activity","volume":"175","author":"Brickman","year":"1993","unstructured":"Brickman, T., Barry, C., Hackstadt, T. Molecular cloning and expression of hctB encoding a strain-variant chlamydial histone-like protein with DNA-binding activity. Journal of Bacteriology. 175 (14), 4274-4281 (1993).","journal-title":"Journal of Bacteriology"},{"issue":"48","key":"ref12","doi-asserted-by":"crossref","first-page":"19781","DOI":"10.1073\/pnas.1212831109","type":"journal-article","article-title":"Developmental stage-specific metabolic and transcriptional activity of Chlamydia trachomatis in an axenic medium","volume":"109","author":"Omsland","year":"2012","unstructured":"Omsland, A., Sager, J., Nair, V., Sturdevant, D., Hackstadt, T. Developmental stage-specific metabolic and transcriptional activity of Chlamydia trachomatis in an axenic medium. Proceedings of the National Academy of Sciences U. S. A. 109 (48), 19781\u201319785 (2012).","journal-title":"Proceedings of the National Academy of Sciences U. S. A"},{"issue":"20","key":"ref13","doi-asserted-by":"crossref","first-page":"11143","DOI":"10.1073\/pnas.93.20.11143","type":"journal-article","article-title":"A recombinant Chlamydia trachomatis major outer membrane protein binds to heparan sulfate receptors on epithelial cells","volume":"93","author":"Su","year":"1996","unstructured":"Su, H. et al. A recombinant Chlamydia trachomatis major outer membrane protein binds to heparan sulfate receptors on epithelial cells. Proceedings of the National Academy of Sciences U.S.A. 93 (20), 11143-11148 (1996).","journal-title":"Proceedings of the National Academy of Sciences U.S.A"},{"key":"ref14","type":"book","article-title":"Computer control of microscopes using \u00b5Manager","volume-title":"Current Protocols in Molecular Biology","author":"Edelstein","year":"2010","unstructured":"Edelstein, A. et al. Computer control of microscopes using \u00b5Manager. Current Protocols in Molecular Biology. Chapter 14-20 (2010)."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.ymeth.2016.09.016","type":"journal-article","article-title":"TrackMate: An open and extensible platform for single-particle tracking","volume":"115","author":"Tinevez","year":"2017","unstructured":"Tinevez, J. Y., et al. TrackMate: An open and extensible platform for single-particle tracking. Methods. 115, 80-90 (2017).","journal-title":"Methods"},{"key":"ref16","volume-title":"Collaborative data science","year":"2015","unstructured":"Plotly Technologies Inc. Collaborative data science. Montr\u00e9al, QC, https:\/\/plot.ly. (2015).","type":"book"},{"issue":"10","key":"ref17","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1038\/nmeth.2171","type":"journal-article","article-title":"Improving FRET dynamic range with bright green and red fluorescent proteins","volume":"9","author":"Lam","year":"2012","unstructured":"Lam, A. J., et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nature Methods. 9 (10), 1005-1012 (2012).","journal-title":"Nature Methods"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1042\/BJ20081949","type":"journal-article","article-title":"Far-red fluorescent tags for protein imaging in living tissues","volume":"418","author":"Shcherbo","year":"2009","unstructured":"Shcherbo, D., et al. Far-red fluorescent tags for protein imaging in living tissues. Biochemical Journal. 418 (3), 567\u2013574 (2009).","journal-title":"Biochemical Journal"},{"issue":"2-3","key":"ref19","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0165-1110(84)90007-1","type":"journal-article","article-title":"A review of the genetic effects of ethyl methanesulfonate","volume":"134","author":"Sega","year":"1984","unstructured":"Sega, G. A review of the genetic effects of ethyl methanesulfonate. Mutation Research. 134 (2-3), 113-142 (1984).","journal-title":"Mutation Research"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/mutage\/5.6.529","type":"journal-article","article-title":"Frameshift mutagenesis by acridines and other reversibly binding DNA ligands","volume":"5","author":"Ferguson","year":"1990","unstructured":"Ferguson, L., Denny, W. Frameshift mutagenesis by acridines and other reversibly binding DNA ligands. Mutagenesis. 5 (6), 529\u2013540 (1990).","journal-title":"Mutagenesis"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61365\/live-cell-forward-genetic-approach-to-identify-isolate-developmental","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:56:53Z","timestamp":1792623413000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61365"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,10]]},"references-count":20,"journal-issue":{"issue":"160","published-online":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.3791\/61365","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,10]]}}}