{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:38:08Z","timestamp":1792625888457,"version":"4.5.0"},"reference-count":34,"publisher":"MyJove Corporation","issue":"161","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61400","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T20:00:06Z","timestamp":1594756806000},"source":"Crossref","is-referenced-by-count":8,"title":["&lt;em&gt;Neisseria meningitidis&lt;\/em&gt; Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells"],"prefix":"10.3791","author":[{"given":"Leo  M.","family":"Endres","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Schubert-Unkmeir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon J.","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.molmed.2018.04.003","type":"journal-article","article-title":"Advances in Meningeal Immunity","volume":"24","author":"Rua","year":"2018","unstructured":"Rua, R., McGavern, D.B. Advances in Meningeal Immunity. Trends in Molecular Medicine. 24 (6), 542-559, (2018).","journal-title":"Trends in Molecular Medicine"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.nbd.2009.07.030","type":"journal-article","article-title":"Structure and function of the blood-brain barrier","volume":"37","author":"Abbott","year":"2010","unstructured":"Abbott, N.J., Patabendige, A.A.K., Dolman, D.E.M., Yusof, S.R., Begley, D.J. Structure and function of the blood-brain barrier. Neurobiology of Disease. 37 (1), 13-25, (2010).","journal-title":"Neurobiology of Disease"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-61779-346-2_1","type":"journal-article","article-title":"Neisseria meningitidis: Biology, microbiology, and epidemiology","author":"Rouphael","year":"2012","unstructured":"Rouphael, N.G., Stephens, D.S. Neisseria meningitidis: Biology, microbiology, and epidemiology. Methods in Molecular Biology. (2012).","journal-title":"Methods in Molecular Biology"},{"issue":"9580","key":"ref4","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.1016\/S0140-6736(07)61016-2","type":"journal-article","article-title":"Epidemic meningitis, meningococcaemia, and Neisseria meningitidis","volume":"369","author":"Stephens","year":"2007","unstructured":"Stephens, D.S., Greenwood, B., Brandtzaeg, P. Epidemic meningitis, meningococcaemia, and Neisseria meningitidis. Lancet. 369 (9580), 2196-2210, (2007).","journal-title":"Lancet"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.13132","type":"journal-article","article-title":"Strategies used by bacterial pathogens to cross the blood-brain barrier","author":"Le Guennec","year":"2020","unstructured":"Le Guennec, L., Coureuil, M., Nassif, X., Bourdoulous, S. Strategies used by bacterial pathogens to cross the blood\u2013brain barrier. Cellular Microbiology. (2020).","journal-title":"Cellular Microbiology"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s00401-015-1531-z","type":"journal-article","article-title":"Host-pathogen interactions in bacterial meningitis","volume":"131","author":"Doran","year":"2016","unstructured":"Doran, K.S. et al. Host\u2013pathogen interactions in bacterial meningitis. Acta Neuropathologica. 131 (2), 185-209, (2016).","journal-title":"Acta Neuropathologica"},{"key":"ref7","type":"journal-article","article-title":"Neisseria meningitidis opc invasin binds to the sulphated tyrosines of activated vitronectin to attach to and invade human brain endothelial cells","author":"Cunha","year":"2010","unstructured":"Cunha, C.S.E., Griffiths, N.J., Virji, M. Neisseria meningitidis opc invasin binds to the sulphated tyrosines of activated vitronectin to attach to and invade human brain endothelial cells. PLoS Pathogens. (2010).","journal-title":"PLoS Pathogens"},{"issue":"7","key":"ref8","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.cell.2010.11.035","type":"journal-article","article-title":"Meningococcus hijacks a \u03b22-adrenoceptor\/\u03b2-arrestin pathway to cross brain microvasculature endothelium","volume":"143","author":"Coureuil","year":"2010","unstructured":"Coureuil, M. et al. Meningococcus hijacks a \u03b22-adrenoceptor\/\u03b2-arrestin pathway to cross brain microvasculature endothelium. Cell. 143 (7), 1149-1160, (2010).","journal-title":"Cell"},{"issue":"7","key":"ref9","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nm.3563","type":"journal-article","article-title":"Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization","volume":"20","author":"Bernard","year":"2014","unstructured":"Bernard, S.C. et al. Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization. Nature Medicine. 20 (7), 725-731, (2014).","journal-title":"Nature Medicine"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0039613","type":"journal-article","article-title":"Cell invasion by neisseria meningitidis requires a functional interplay between the focal adhesion kinase, Src and cortactin","volume":"7","author":"Slanina","year":"2012","unstructured":"Slanina, H., Hebling, S., Hauck, C.R., Schubert-Unkmeir, A. Cell invasion by neisseria meningitidis requires a functional interplay between the focal adhesion kinase, Src and cortactin. PLoS ONE. 7 (6), (2012).","journal-title":"PLoS ONE"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1046\/j.1365-2958.2002.03222.x","type":"journal-article","article-title":"Fibronectin mediates Opc-dependent internalization of Neisseria meningitidis in human brain microvascular endothelial cells","volume":"46","author":"Unkmeir","year":"2002","unstructured":"Unkmeir, A. et al. Fibronectin mediates Opc-dependent internalization of Neisseria meningitidis in human brain microvascular endothelial cells. Molecular Microbiology. 46 (4), 933-946, (2002).","journal-title":"Molecular Microbiology"},{"key":"ref12","type":"journal-article","article-title":"In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use","author":"Helms","year":"2015","unstructured":"Helms, H.C. et al. In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use. Journal of Cerebral Blood Flow and Metabolism. (2015).","journal-title":"Journal of Cerebral Blood Flow and Metabolism"},{"issue":"8","key":"ref13","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nbt.2247","type":"journal-article","article-title":"Derivation of Blood-Brain Barrier Endothelial Cells from Human Pluripotent Stem Cells","volume":"30","author":"Lippmann","year":"2012","unstructured":"Lippmann, E.S. et al. Derivation of Blood-Brain Barrier Endothelial Cells from Human Pluripotent Stem Cells. Nature Biotechnology. 30 (8), 783-91, (2012).","journal-title":"Nature Biotechnology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"4160","DOI":"10.1038\/srep04160","type":"journal-article","article-title":"A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources","volume":"4","author":"Lippmann","year":"2014","unstructured":"Lippmann, E.S., Al-Ahmad, A., Azarin, S.M., Palecek, S.P., Shusta, E. V A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources. Scientific Reports. 4 (February 2014), 4160, (2014).","journal-title":"Scientific Reports"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1186\/s12987-017-0059-0","type":"journal-article","article-title":"Accelerated differentiation of human induced pluripotent stem cells to blood-brain barrier endothelial cells","author":"Hollmann","year":"2017","unstructured":"Hollmann, E.K., Bailey, A.K., Potharazu, A. V., Neely, M.D., Bowman, A.B., Lippmann, E.S. Accelerated differentiation of human induced pluripotent stem cells to blood-brain barrier endothelial cells. Fluids and Barriers of the CNS. (2017).","journal-title":"Fluids and Barriers of the CNS"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00398-17","type":"journal-article","article-title":"Modeling Group B Streptococcus and Blood-Brain Barrier Interaction by Using Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells","author":"Kim","year":"2017","unstructured":"Kim, B.J. et al. Modeling Group B Streptococcus and Blood-Brain Barrier Interaction by Using Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. mSphere. (2017).","journal-title":"mSphere"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s12987-019-0146-5","type":"journal-article","article-title":"Streptococcus agalactiae disrupts P-glycoprotein function in brain endothelial cells","volume":"16","author":"Kim","year":"2019","unstructured":"Kim, B.J. et al. Streptococcus agalactiae disrupts P-glycoprotein function in brain endothelial cells. Fluids and Barriers of the CNS. 16 (1), 26, (2019).","journal-title":"Fluids and Barriers of the CNS"},{"key":"ref18","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2019.01336","type":"journal-article","article-title":"Past and Current Perspectives in Modeling Bacteria and Blood\u2013Brain Barrier Interactions","author":"Kim","year":"2019","unstructured":"Kim, B.J., Shusta, E. V., Doran, K.S. Past and Current Perspectives in Modeling Bacteria and Blood\u2013Brain Barrier Interactions. Frontiers in Microbiology. (2019).","journal-title":"Frontiers in Microbiology"},{"key":"ref19","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2019.01181","type":"journal-article","article-title":"Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells as a Cellular Model to Study Neisseria meningitidis Infection","author":"Martins Gomes","year":"2019","unstructured":"Martins Gomes, S.F. et al. Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells as a Cellular Model to Study Neisseria meningitidis Infection. Frontiers in Microbiology. (2019).","journal-title":"Frontiers in Microbiology"},{"key":"ref20","type":"journal-article","article-title":"Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier","author":"Vatine","year":"2016","unstructured":"Vatine, G.D. et al. Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier. Cell Stem Cell. (2016).","journal-title":"Cell Stem Cell"},{"issue":"7","key":"ref21","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1016\/j.celrep.2017.04.021","type":"journal-article","article-title":"Huntington's Disease iPSC-Derived Brain Microvascular Endothelial Cells Reveal WNT-Mediated Angiogenic and Blood-Brain Barrier Deficits","volume":"19","author":"Lim","year":"2017","unstructured":"Lim, R.G. et al. Huntington\u2019s Disease iPSC-Derived Brain Microvascular Endothelial Cells Reveal WNT-Mediated Angiogenic and Blood-Brain Barrier Deficits. Cell Reports. 19 (7), 1365-1377, (2017).","journal-title":"Cell Reports"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ymeth.2015.10.016","type":"journal-article","article-title":"Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells","volume":"101","author":"Stebbins","year":"2016","unstructured":"Stebbins, M.J., Wilson, H.K., Canfield, S.G., Qian, T., Palecek, S.P., Shusta, E. V. Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells. Methods. 101, 93-102, (2016).","journal-title":"Methods"},{"issue":"6","key":"ref23","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1016\/j.stemcr.2019.05.008","type":"journal-article","article-title":"A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs","volume":"12","author":"Neal","year":"2019","unstructured":"Neal, E.H. et al. A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs. Stem Cell Reports. 12 (6), 1380-1388, (2019).","journal-title":"Stem Cell Reports"},{"key":"ref24","doi-asserted-by":"crossref","DOI":"10.1186\/s12987-015-0007-9","type":"journal-article","article-title":"Exploring the effects of cell seeding density on the differentiation of human pluripotent stem cells to brain microvascular endothelial cells","author":"Wilson","year":"2015","unstructured":"Wilson, H.K., Canfield, S.G., Hjortness, M.K., Palecek, S.P., Shusta, E. V. Exploring the effects of cell seeding density on the differentiation of human pluripotent stem cells to brain microvascular endothelial cells. Fluids and Barriers of the CNS. (2015).","journal-title":"Fluids and Barriers of the CNS"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.1089\/ten.tec.2016.0345","type":"journal-article","article-title":"Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by rho-associated coiled coil-containing kinase inhibition","author":"Wilson","year":"2016","unstructured":"Wilson, H.K., Faubion, M.G., Hjortness, M.K., Palecek, S.P., Shusta, E. V. Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by rho-associated coiled coil-containing kinase inhibition. Tissue Engineering - Part C: Methods. (2016).","journal-title":"Tissue Engineering - Part C: Methods"},{"key":"ref26","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1701679","type":"journal-article","article-title":"Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells","author":"Qian","year":"2017","unstructured":"Qian, T. et al. Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells. Science Advances. (2017).","journal-title":"Science Advances"},{"key":"ref27","doi-asserted-by":"crossref","DOI":"10.1016\/j.stemcr.2018.02.012","type":"journal-article","article-title":"Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development","author":"Sances","year":"2018","unstructured":"Sances, S. et al. Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development. Stem Cell Reports. (2018).","journal-title":"Stem Cell Reports"},{"key":"ref28","doi-asserted-by":"crossref","DOI":"10.1111\/jnc.13923","type":"journal-article","article-title":"An isogenic blood-brain barrier model comprising brain endothelial cells, astrocytes, and neurons derived from human induced pluripotent stem cells","author":"Canfield","year":"2017","unstructured":"Canfield, S.G. et al. An isogenic blood\u2013brain barrier model comprising brain endothelial cells, astrocytes, and neurons derived from human induced pluripotent stem cells. Journal of Neurochemistry. (2017).","journal-title":"Journal of Neurochemistry"},{"key":"ref29","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbiosc.2012.07.013","type":"journal-article","article-title":"Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells","author":"Kurosawa","year":"2012","unstructured":"Kurosawa, H. Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells. Journal of Bioscience and Bioengineering. (2012).","journal-title":"Journal of Bioscience and Bioengineering"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1128\/IAI.01508-06","type":"journal-article","article-title":"Gene expression pattern in human brain endothelial cells in response to Neisseria meningitidis","volume":"75","author":"Schubert-Unkmeir","year":"2007","unstructured":"Schubert-Unkmeir, A., Sokolova, O., Panzner, U., Eigenthaler, M., Frosch, M. Gene expression pattern in human brain endothelial cells in response to Neisseria meningitidis. Infection and Immunity. 75 (2), 899-914, (2007).","journal-title":"Infection and Immunity"},{"issue":"12","key":"ref31","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1111\/j.1462-5822.2004.00422.x","type":"journal-article","article-title":"Interaction of Neisseria meningitidis with human brain microvascular endothelial cells: Role of MAP- and tyrosine kinases in invasion and inflammatory cytokine release","volume":"6","author":"Sokolova","year":"2004","unstructured":"Sokolova, O. et al. Interaction of Neisseria meningitidis with human brain microvascular endothelial cells: Role of MAP- and tyrosine kinases in invasion and inflammatory cytokine release. Cellular Microbiology. 6 (12), 1153-1166, (2004).","journal-title":"Cellular Microbiology"},{"key":"ref32","doi-asserted-by":"crossref","DOI":"10.1186\/s12987-018-0100-y","type":"journal-article","article-title":"Zika virus crosses an in vitro human blood brain barrier model","author":"Alimonti","year":"2018","unstructured":"Alimonti, J.B. et al. Zika virus crosses an in vitro human blood brain barrier model. Fluids and Barriers of the CNS. (2018).","journal-title":"Fluids and Barriers of the CNS"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/978-1-4939-9202-7_10","type":"journal-article","article-title":"In vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells","volume":"1969","author":"Kim","year":"2019","unstructured":"Kim, B.J., Schubert-unkmeir, A. In vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells. Neisseria meningitidis: Methods and Protocols, Methods in Molecular Biology. 1969, 135-148, (2019).","journal-title":"Neisseria meningitidis: Methods and Protocols, Methods in Molecular Biology"},{"issue":"6","key":"ref34","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.1172\/JCI74159","type":"journal-article","article-title":"Bacterial induction of Snail1 contributes to blood-brain barrier disruption","volume":"125","author":"Kim","year":"2015","unstructured":"Kim, B.J. et al. Bacterial induction of Snail1 contributes to blood-brain barrier disruption. Journal of Clinical Investigation. 125 (6), 2473-2483, (2015).","journal-title":"Journal of Clinical Investigation"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61400\/neisseria-meningitidis-infection-induced-pluripotent-stem-cell","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:46:49Z","timestamp":1792622809000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61400"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,14]]},"references-count":34,"journal-issue":{"issue":"161","published-online":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.3791\/61400","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,14]]}}}