{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:38:04Z","timestamp":1792625884971,"version":"4.5.0"},"reference-count":29,"publisher":"MyJove Corporation","issue":"161","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61426","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T20:00:22Z","timestamp":1593806422000},"source":"Crossref","is-referenced-by-count":64,"title":["<em>Plasmodium falciparum<\/em> Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding"],"prefix":"10.3791","author":[{"given":"Abhai  K","family":"Tripathi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Godfree","family":"Mlambo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachie","family":"Kanatani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Photini","family":"Sinnis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Dimopoulos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2020,7,3]]},"reference":[{"key":"ref1","type":"journal-article","article-title":"World Malaria Report","year":"2018","unstructured":"World Health Organization, World Malaria Report. WHO. (2018).","journal-title":"World Health Organization"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1017\/S003118200005335X","type":"journal-article","article-title":"Gametocytogenesis of Plasmodium falciparum in vitro: The cell-cycle","volume":"79","author":"Sinden","year":"1979","unstructured":"Sinden, R. E., Smalley, M. E. Gametocytogenesis of Plasmodium falciparum in vitro: The cell-cycle. Parasitology. 79, (2), 277-296 (1979).","journal-title":"Parasitology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0065-308X(08)60462-5","type":"journal-article","article-title":"Sexual Development of Malarial Parasites","volume":"22","author":"Sinden","year":"1983","unstructured":"Sinden, R. E. Sexual Development of Malarial Parasites. Advances in Parasitology. 22, 153-216 (1983).","journal-title":"Advances in Parasitology"},{"issue":"244","key":"ref4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1126\/scitranslmed.3008882","type":"journal-article","article-title":"Plasmodium falciparum transmission stages accumulate in the human bone marrow","volume":"6","author":"Joice","year":"2014","unstructured":"Joice, R. et al. Plasmodium falciparum transmission stages accumulate in the human bone marrow. Science Translational Medicine. 6, (244), 244re5 (2014).","journal-title":"Science Translational Medicine"},{"issue":"12","key":"ref5","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1002\/ajh.21796","type":"journal-article","article-title":"Immature Plasmodium falciparum gametocytes in bone marrow","volume":"85","author":"Abdulsalam","year":"2010","unstructured":"Abdulsalam, A. H., Sabeeh, N., Bain, B. J. Immature Plasmodium falciparum gametocytes in bone marrow. American Journal of Hematology. 85, (12), 943 (2010).","journal-title":"American Journal of Hematology"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.mib.2009.06.010","type":"journal-article","article-title":"Plasmodium sporozoite invasion of the mosquito salivary gland","volume":"12","author":"Ghosh","year":"2009","unstructured":"Ghosh, A. K., Jacobs-Lorena, M. Plasmodium sporozoite invasion of the mosquito salivary gland. Current Opinion in Microbiology. 12 (4), 394-400 (2009).","journal-title":"Current Opinion in Microbiology"},{"issue":"7","key":"ref7","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1111\/cmi.12604","type":"journal-article","article-title":"The development of malaria parasites in the mosquito midgut","volume":"18","author":"Bennink","year":"2016","unstructured":"Bennink, S., Kiesow, M. J., Pradel, G. The development of malaria parasites in the mosquito midgut. Cellular Microbiology. 18, (7), 905-918 (2016).","journal-title":"Cellular Microbiology"},{"issue":"5664","key":"ref8","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/273621a0","type":"journal-article","article-title":"Cultivation of malarial parasites","volume":"273","author":"Trager","year":"1978","unstructured":"Trager, W., Jenson, J. B. Cultivation of malarial parasites. Nature. 273, (5664), 621-622 (1978).","journal-title":"Nature"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1038\/nprot.2016.056","type":"journal-article","article-title":"Large-scale production of Plasmodium falciparum gametocytes for malaria drug discovery","volume":"11","author":"Duffy","year":"2016","unstructured":"Duffy, S., Loganathan, S., Holleran, J. P., Avery, V. M. Large-scale production of Plasmodium falciparum gametocytes for malaria drug discovery. Nature Protocols. 11, (5), 976-992 (2016).","journal-title":"Nature Protocols"},{"issue":"9","key":"ref10","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1038\/nprot.2016.096","type":"journal-article","article-title":"Routine in vitro culture of P. Falciparum gametocytes to evaluate novel transmission-blocking interventions","volume":"11","author":"Delves","year":"2016","unstructured":"Delves, M. J. et al. Routine in vitro culture of P. Falciparum gametocytes to evaluate novel transmission-blocking interventions. Nature Protocols. 11, (9), 1668-1680 (2016).","journal-title":"Nature Protocols"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12936-019-2663-8","type":"journal-article","article-title":"Streamlined SMFA and mosquito dark-feeding regime significantly improve malaria transmission-blocking assay robustness and sensitivity","volume":"18","author":"Habtewold","year":"2019","unstructured":"Habtewold, T. et al. Streamlined SMFA and mosquito dark-feeding regime significantly improve malaria transmission-blocking assay robustness and sensitivity. Malaria Journal. 18, (1), 24 (2019).","journal-title":"Malaria Journal"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/s13071-017-2155-z","type":"journal-article","article-title":"The development of sexual stage malaria gametocytes in a Wave Bioreactor","volume":"10","author":"Demanga","year":"2017","unstructured":"Demanga, C. G. et al. The development of sexual stage malaria gametocytes in a Wave Bioreactor. Parasites and Vectors. 10, (1), 216 (2017).","journal-title":"Parasites and Vectors"},{"key":"ref13","first-page":"454","type":"journal-article","article-title":"Conditions favoring gametocytogenesis in the continuous culture of Plasmodium falciparum","volume":"29","author":"Brockelman","year":"1982","unstructured":"Brockelman, C. R. Conditions favoring gametocytogenesis in the continuous culture of Plasmodium falciparum. Journal of Eukaryotic Microbiology. 29, 454\u2013458 (1982).","journal-title":"Journal of Eukaryotic Microbiology"},{"key":"ref14","type":"book","article-title":"Biology of malaria transmission","volume-title":"Cold Spring Harbor Perspectives in Medicine","author":"Meibalan","year":"2017","unstructured":"Meibalan, E., Marti, M. Biology of malaria transmission. Cold Spring Harbor Perspectives in Medicine. 7, (2017)."},{"issue":"10","key":"ref15","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1093\/infdis\/jix442","type":"journal-article","article-title":"A novel gametocyte biomarker for superior molecular detection of the plasmodium falciparum infectious reservoirs","volume":"216","author":"Essuman","year":"2017","unstructured":"Essuman, E. et al. A novel gametocyte biomarker for superior molecular detection of the plasmodium falciparum infectious reservoirs. Journal of Infectious Diseases. 216, (10), 1264-1272 (2017).","journal-title":"Journal of Infectious Diseases"},{"issue":"5","key":"ref16","doi-asserted-by":"crossref","first-page":"01631","DOI":"10.1128\/mBio.01631-17","type":"journal-article","article-title":"Immune regulation of plasmodium is anopheles species specific and infection intensity dependent","volume":"8","author":"Sim\u00f5es","year":"2017","unstructured":"Sim\u00f5es, M. L., Mlambo, G., Tripathi, A., Dong, Y., Dimopoulos, G. Immune regulation of plasmodium is anopheles species specific and infection intensity dependent. mBio. 8, (5), e01631- e01617 (2017).","journal-title":"mBio"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"11568","DOI":"10.1038\/s41598-018-29969-4","type":"journal-article","article-title":"Transcriptome analysis based detection of Plasmodium falciparum development in Anopheles stephensi mosquitoes","volume":"8","author":"Oakley","year":"2018","unstructured":"Oakley, M. S. et al. Transcriptome analysis based detection of Plasmodium falciparum development in Anopheles stephensi mosquitoes. Scientific Reports. 8, 11568 (2018).","journal-title":"Scientific Reports"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"6176","DOI":"10.1038\/s41598-018-24296-0","type":"journal-article","article-title":"Chromobacterium spp. mediate their anti-Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin","volume":"8","author":"Saraiva","year":"2018","unstructured":"Saraiva, R. G. et al. Chromobacterium spp. mediate their anti-Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin. Scientific Reports. 8, 6176 (2018).","journal-title":"Scientific Reports"},{"issue":"10","key":"ref19","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1074\/mcp.M114.040956","type":"journal-article","article-title":"Sex-partitioning of the Plasmodium falciparum stage V gametocyte proteome provides insight into falciparum-specific cell biology","volume":"13","author":"Tao","year":"2014","unstructured":"Tao, D. et al. Sex-partitioning of the Plasmodium falciparum stage V gametocyte proteome provides insight into falciparum-specific cell biology. Molecular and Cellular Proteomics. 13, (10), 2705-2724 (2014).","journal-title":"Molecular and Cellular Proteomics"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.mimet.2014.10.006","type":"journal-article","article-title":"A sensitive enhanced chemiluminescent-ELISA for the detection of Plasmodium falciparum circumsporozoite antigen in midguts of Anopheles stephensi mosquitoes","volume":"108","author":"Grabias","year":"2015","unstructured":"Grabias, B., Zheng, H., Mlambo, G., Tripathi, A. K., Kumar, S. A sensitive enhanced chemiluminescent-ELISA for the detection of Plasmodium falciparum circumsporozoite antigen in midguts of Anopheles stephensi mosquitoes. Journal of Microbiological Methods. 108, 19-24 (2015).","journal-title":"Journal of Microbiological Methods"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1128\/AAC.04642-14","type":"journal-article","article-title":"Antimalarial iron chelator FBS0701 blocks transmission by Plasmodium falciparum gametocyte activation inhibition","volume":"59","author":"Ferrer","year":"2015","unstructured":"Ferrer, P., Vega-Rodriguez, J., Tripathi, A. K., Jacobs-Lorena, M., Sullivan, D. J. Antimalarial iron chelator FBS0701 blocks transmission by Plasmodium falciparum gametocyte activation inhibition. Antimicrobial Agents and Chemotherapy. 59, (3), 1418-1426 (2015).","journal-title":"Antimicrobial Agents and Chemotherapy"},{"issue":"8","key":"ref22","doi-asserted-by":"crossref","first-page":"105817","DOI":"10.1371\/journal.pone.0105817","type":"journal-article","article-title":"Gametocytocidal screen identifies novel chemical classes with Plasmodium falciparum transmission blocking activity","volume":"9","author":"Sanders","year":"2014","unstructured":"Sanders, N. G., Sullivan, D. J., Mlambo, G., Dimopoulos, G., Tripathi, A. K. Gametocytocidal screen identifies novel chemical classes with Plasmodium falciparum transmission blocking activity. PLoS One. 9, (8), e105817 (2014).","journal-title":"PLoS One"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"4964","DOI":"10.1038\/s41467-019-12936-6","type":"journal-article","article-title":"Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites","volume":"10","author":"Lindner","year":"2019","unstructured":"Lindner, S. E. et al. Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites. Nature Communications. 10, 4964 (2019).","journal-title":"Nature Communications"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"13131","DOI":"10.1038\/s41598-019-49348-x","type":"journal-article","article-title":"Generation of Transmission-Competent Human Malaria Parasites with Chromosomally-Integrated Fluorescent Reporters","volume":"9","author":"McLean","year":"2019","unstructured":"McLean, K. J. et al. Generation of Transmission-Competent Human Malaria Parasites with Chromosomally-Integrated Fluorescent Reporters. Scientific Reports. 9, 13131 (2019).","journal-title":"Scientific Reports"},{"issue":"7","key":"ref25","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/infdis\/jiv154","type":"journal-article","article-title":"Proteolytic Cleavage of the Plasmodium falciparum Circumsporozoite Protein Is a Target of Protective Antibodies","volume":"212","author":"Espinosa","year":"2015","unstructured":"Espinosa, D. A. et al. Proteolytic Cleavage of the Plasmodium falciparum Circumsporozoite Protein Is a Target of Protective Antibodies. Journal of Infectious Diseases. 212, (7), 1111-1119 (2015).","journal-title":"Journal of Infectious Diseases"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"1005606","DOI":"10.1371\/journal.ppat.1005606","type":"journal-article","article-title":"Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics","volume":"12","author":"Swearingen","year":"2016","unstructured":"Swearingen, K. E. et al. Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics. PLoS Pathogens. 12, (4), e1005606 (2016).","journal-title":"PLoS Pathogens"},{"issue":"5839","key":"ref27","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/294364a0","type":"journal-article","article-title":"Complete in vitro maturation of Plasmodium falciparum gametocytes","volume":"294","author":"Ifediba","year":"1981","unstructured":"Ifediba, T., Vanderberg, J. P. Complete in vitro maturation of Plasmodium falciparum gametocytes. Nature. 294, (5839), 364-366 (1981).","journal-title":"Nature"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1186\/s12936-016-1515-z","type":"journal-article","article-title":"An inter-laboratory comparison of standard membrane-feeding assays for evaluation of malaria transmission-blocking vaccines","volume":"15","author":"Miura","year":"2016","unstructured":"Miura, K. et al. An inter-laboratory comparison of standard membrane-feeding assays for evaluation of malaria transmission-blocking vaccines. Malaria Journal. 15, 463 (2016).","journal-title":"Malaria Journal"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"57909","DOI":"10.1371\/journal.pone.0057909","type":"journal-article","article-title":"Qualification of Standard Membrane-Feeding Assay with Plasmodium falciparum Malaria and Potential Improvements for Future Assays","volume":"8","author":"Miura","year":"2013","unstructured":"Miura, K. et al. Qualification of Standard Membrane-Feeding Assay with Plasmodium falciparum Malaria and Potential Improvements for Future Assays. PLoS One. 8, (3), e57909 (2013).","journal-title":"PLoS One"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61426\/plasmodium-falciparum-gametocyte-culture-mosquito-infection-through","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:47:53Z","timestamp":1792622873000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,3]]},"references-count":29,"journal-issue":{"issue":"161","published-online":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.3791\/61426","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,3]]}}}