{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:38:32Z","timestamp":1792618712831,"version":"4.5.0"},"reference-count":33,"publisher":"MyJove Corporation","issue":"176","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61470","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T20:44:07Z","timestamp":1634244247000},"source":"Crossref","is-referenced-by-count":0,"title":["Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from S&amp;#233;zary Syndrome Patients for Transcriptomic Profiling"],"prefix":"10.3791","author":[{"given":"Syed  J.","family":"Mehdi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea  M.","family":"Moerman-Herzog","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry K.","family":"Wong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,10,14]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1172\/JCI24826","type":"journal-article","article-title":"Immunopathogenesis and therapy of cutaneous T cell lymphoma","volume":"115","author":"Kim","year":"2005","unstructured":"Kim, E.J. et al. Immunopathogenesis and therapy of cutaneous T cell lymphoma. Journal of Clinical Investigation. 115, 798-812 (2005).","journal-title":"Journal of Clinical Investigation"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/j.1365-2141.2011.08852.x","type":"journal-article","article-title":"Evolving Insights in the Pathogenesis and Therapy of Cutaneous T-cell lymphoma (Mycosis Fungoides and Sezary Syndrome)","volume":"155","author":"Wong","year":"2011","unstructured":"Wong, H.K. et al. Evolving Insights in the Pathogenesis and Therapy of Cutaneous T-cell lymphoma (Mycosis Fungoides and Sezary Syndrome). British Journal of Haematology. 155 (2), 150-166 (2011).","journal-title":"British Journal of Haematology"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1053\/j.seminoncol.2005.12.015","type":"journal-article","article-title":"Biological insights into the pathogenesis of cutaneous T-cell lymphomas (CTCL)","volume":"33","author":"Whittaker","year":"2006","unstructured":"Whittaker, S. Biological insights into the pathogenesis of cutaneous T-cell lymphomas (CTCL). Seminars in oncology. 33 (3), 3-6 (2006).","journal-title":"Seminars in oncology"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1046\/j.1523-1747.2003.12555.x","type":"journal-article","article-title":"Chemokine receptor expression on neoplastic and reactive T cells in the skin at different stages of Mucosis Fungoides","volume":"121","author":"Kallinich","year":"2003","unstructured":"Kallinich, T. et al. Chemokine receptor expression on neoplastic and reactive T cells in the skin at different stages of Mucosis Fungoides. Journal of Investigative Dermatology. 121 (5), 1045-1052 (2003).","journal-title":"Journal of Investigative Dermatology"},{"key":"ref5","first-page":"1","type":"journal-article","article-title":"Current and Emerging Therapeutics for Cutaneous T-Cell Lymphoma: Histone Deacetylase Inhibitors","volume":"2012","author":"Rodd","year":"2012","unstructured":"Rodd, A.L. et al. Current and Emerging Therapeutics for Cutaneous T-Cell Lymphoma: Histone Deacetylase Inhibitors. Lymphoma. 2012, 1-10, Article ID 290685 (2012).","journal-title":"Lymphoma"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1182\/blood-2007-03-055749","type":"journal-article","article-title":"Revisions to the staging and classification of mycosis fungoides and Sezary syndrome: a proposal of the International Society for Cutaneous Lymphomas (ISCL) and the cutaneous lymphoma task force of the European Organization of Research and Treatment of Cancer (EORTC)","volume":"110","author":"Olsen","year":"2007","unstructured":"Olsen, E. et al. Revisions to the staging and classification of mycosis fungoides and Sezary syndrome: a proposal of the International Society for Cutaneous Lymphomas (ISCL) and the cutaneous lymphoma task force of the European Organization of Research and Treatment of Cancer (EORTC). Blood. 110, 1713-1722 (2007).","journal-title":"Blood"},{"issue":"31","key":"ref7","doi-asserted-by":"crossref","first-page":"31868","DOI":"10.18632\/oncotarget.5573","type":"journal-article","article-title":"EPHA4 is overexpressed but not functionally active in S\u00e9zary syndrome","volume":"6","author":"Hameetman","year":"2015","unstructured":"Hameetman, L. et al. EPHA4 is overexpressed but not functionally active in S\u00e9zary syndrome. Oncotarget. 6 (31), 31868-31876 (2015).","journal-title":"Oncotarget"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3768","DOI":"10.1182\/blood-2004-09-3502","type":"journal-article","article-title":"WHO-EORTC classification for cutaneous lymphomas","volume":"105","author":"Willemze","year":"2005","unstructured":"Willemze, R. et al. WHO-EORTC classification for cutaneous lymphomas. Blood. 105, 3768-3785 (2005).","journal-title":"Blood"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"5064","DOI":"10.1182\/blood-2008-10-184168","type":"journal-article","article-title":"Cutaneous lymphoma incidence patterns in the United States: a population-based study of 3884 cases","volume":"113","author":"Bradford","year":"2009","unstructured":"Bradford, P.T. et al. Cutaneous lymphoma incidence patterns in the United States: a population-based study of 3884 cases. Blood. 113, 5064-5073 (2009).","journal-title":"Blood"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.clml.2012.06.010","type":"journal-article","article-title":"Age, race, sex, stage, and incidence of cutaneous lymphoma","volume":"12","author":"Wilson","year":"2012","unstructured":"Wilson, L.D. et al. Age, race, sex, stage, and incidence of cutaneous lymphoma. Clinical Lymphoma Myeloma and Leukemia. 12, 291-296 (2012).","journal-title":"Clinical Lymphoma Myeloma and Leukemia"},{"key":"ref11","first-page":"75","type":"journal-article","article-title":"Management of cutaneous T cell lymphoma: new and emerging targets and treatment options","volume":"4","author":"Li","year":"2012","unstructured":"Li, Y. et al. Management of cutaneous T cell lymphoma: new and emerging targets and treatment options. Cancer Management and Research. 4, 75-89 (2012).","journal-title":"Cancer Management and Research"},{"issue":"11","key":"ref12","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1001\/jamadermatol.2013.5526","type":"journal-article","article-title":"Changing incidence trends of cutaneous T-cell lymphoma","volume":"149","author":"Korgavkar","year":"2013","unstructured":"Korgavkar, K. et al. Changing incidence trends of cutaneous T-cell lymphoma. Jama Dertmatology. 149 (11), 1295-1299 (2013).","journal-title":"Jama Dertmatology"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1111\/bjd.12578","type":"journal-article","article-title":"Lessons learned from gene expression profiling of cutaneous T-cell lymphoma","volume":"169","author":"Dulmage","year":"2013","unstructured":"Dulmage, B.O., Geskin, L.J. Lessons learned from gene expression profiling of cutaneous T-cell lymphoma. British Journal of Dermatology. 169 (6), 1188-1197 (2013).","journal-title":"British Journal of Dermatology"},{"issue":"7","key":"ref14","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1016\/j.jid.2016.01.038","type":"journal-article","article-title":"Evaluation of Immunophenotypic and Molecular Biomarkers for S\u00e9zary Syndrome Using Standard Operating Procedures: A Multicenter Study of 59 Patients","volume":"136","author":"Boonk","year":"2016","unstructured":"Boonk, S.E. et al. Evaluation of Immunophenotypic and Molecular Biomarkers for S\u00e9zary Syndrome Using Standard Operating Procedures: A Multicenter Study of 59 Patients. Journal of Investigative Dermatology. 136 (7), 1364-1372 (2016).","journal-title":"Journal of Investigative Dermatology"},{"issue":"33","key":"ref15","doi-asserted-by":"crossref","first-page":"3766","DOI":"10.1200\/JCO.2015.61.7142","type":"journal-article","article-title":"Cutaneous Lymphoma International Consortium Study of Outcome in Advanced Stages of Mycosis Fungoides and S\u00e9zary Syndrome: Effect of Specific Prognostic Markers on Survival and Development of a Prognostic Model","volume":"10","author":"Scarisbrick","year":"2015","unstructured":"Scarisbrick, J.J. et al. Cutaneous Lymphoma International Consortium Study of Outcome in Advanced Stages of Mycosis Fungoides and S\u00e9zary Syndrome: Effect of Specific Prognostic Markers on Survival and Development of a Prognostic Model. Journal of Clinical Oncology. 10 (33), 3766-3773 (2015).","journal-title":"Journal of Clinical Oncology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s00403-016-1698-8","type":"journal-article","article-title":"CD164 identifies CD4+ T cells highly expressing genes associated with malignancy in S\u00e9zary syndrome: the S\u00e9zary signature genes, FCRL3, Tox, and miR-214","volume":"309","author":"Benoit","year":"2017","unstructured":"Benoit, B.M. et al. CD164 identifies CD4+ T cells highly expressing genes associated with malignancy in S\u00e9zary syndrome: the S\u00e9zary signature genes, FCRL3, Tox, and miR-214. Archives of Dermatological Research. 309, 11-19 (2017).","journal-title":"Archives of Dermatological Research"},{"issue":"8","key":"ref17","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1111\/exd.13261","type":"journal-article","article-title":"The biomarker landscape in mycosis fungoides and S\u00e9zary syndrome","volume":"26","author":"Dulmage","year":"2017","unstructured":"Dulmage, B. et al. The biomarker landscape in mycosis fungoides and S\u00e9zary syndrome. Experimental Dermatology. 26 (8), 668-676 (2017).","journal-title":"Experimental Dermatology"},{"issue":"332","key":"ref18","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1111\/j.1749-6632.1979.tb47132.x","type":"journal-article","article-title":"Intracellular Mediation of Lymphokine Action: Mimicry of Migration Inhibitory Factor (MIF) Action by Phorbol Myristate Acetate (PMA) and the Ionophore A23187","volume":"94","author":"Pick","year":"1979","unstructured":"Pick, E. et al. Intracellular Mediation of Lymphokine Action: Mimicry of Migration Inhibitory Factor (MIF) Action by Phorbol Myristate Acetate (PMA) and the Ionophore A23187. Annals of the New York Academy of Sciences. 94 (332), 378-394 (1979).","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jdermsci.2010.07.007","type":"journal-article","article-title":"Induced S\u00e9zary syndrome PBMCs poorly express immune response genes up-regulated in stimulated memory T cells","volume":"60","author":"Chong","year":"2010","unstructured":"Chong, B.F. et al. Induced S\u00e9zary syndrome PBMCs poorly express immune response genes up-regulated in stimulated memory T cells. Journal of Dermatological Science. 60 (1), 8-20 (2010).","journal-title":"Journal of Dermatological Science"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1038\/sj.leu.2400745","type":"journal-article","article-title":"Diminished TCR signaling in cutaneous T cell lymphoma is associated with decreased activities of Zap70, Syk and membrane-associated Csk","volume":"11","author":"Fargnoli","year":"1997","unstructured":"Fargnoli, M.C. et al. Diminished TCR signaling in cutaneous T cell lymphoma is associated with decreased activities of Zap70, Syk and membrane-associated Csk. Leukemia. 11, 1338-1346 (1997).","journal-title":"Leukemia"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/1523-1747.ep12472333","type":"journal-article","article-title":"Leukemic T cells from patients with cutaneous T-cell lymphoma demonstrate enhanced activation through CDw60, CD2, and CD28 relative to activation through the T-cell antigen receptor complex","author":"Hansen","year":"1993","unstructured":"Hansen, E.R. et al. Leukemic T cells from patients with cutaneous T-cell lymphoma demonstrate enhanced activation through CDw60, CD2, and CD28 relative to activation through the T-cell antigen receptor complex. Journal of Investigative Dermatology. 100, 667-673 (1993).","journal-title":"Journal of Investigative Dermatology"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1158\/1078-0432.CCR-07-0610","type":"journal-article","article-title":"Immune Function Abnormalities in Peripheral Blood Mononuclear Cell Cytokine Expression Differentiates Stages of Cutaneous T-Cell Lymphoma\/Mycosis Fungoides","volume":"14","author":"Chong","year":"2008","unstructured":"Chong, B.F. et al. Immune Function Abnormalities in Peripheral Blood Mononuclear Cell Cytokine Expression Differentiates Stages of Cutaneous T-Cell Lymphoma\/Mycosis Fungoides. Clinical Cancer Research. 14 (3), 646-653 (2008).","journal-title":"Clinical Cancer Research"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"5052","DOI":"10.18632\/oncotarget.27120","type":"journal-article","article-title":"Transcriptome analysis of S\u00e9zary syndrome and lymphocytic-variant hypereosinophilic syndrome T cells reveals common and divergent genes","volume":"10","author":"Moerman-Herzog","year":"2019","unstructured":"Moerman-Herzog, A.M. et al. Transcriptome analysis of S\u00e9zary syndrome and lymphocytic-variant hypereosinophilic syndrome T cells reveals common and divergent genes. Oncotarget. 10, 5052-5069 (2019).","journal-title":"Oncotarget"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1016\/j.jid.2017.10.028","type":"journal-article","article-title":"Role of Dysregulated Cytokine Signaling and Bacterial Triggers in the Pathogenesis of Cutaneous T-Cell Lymphoma","volume":"138","author":"Fanok","year":"2018","unstructured":"Fanok, M.H. et al. Role of Dysregulated Cytokine Signaling and Bacterial Triggers in the Pathogenesis of Cutaneous T-Cell Lymphoma. Journal of Investigative Dermatology. 138, 1116-1125 (2018).","journal-title":"Journal of Investigative Dermatology"},{"key":"ref25","first-page":"7122","type":"journal-article","article-title":"Aberrant expression of T-plastin in Sezary cells","volume":"63","author":"Su","year":"2003","unstructured":"Su, M.W. et al. Aberrant expression of T-plastin in Sezary cells. Cancer Research. 63, 7122-7127 (2003).","journal-title":"Cancer Research"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"5578","DOI":"10.1158\/0008-5472.CAN-04-1253","type":"journal-article","article-title":"Aberrant expression of the tyrosine kinase receptor EphA4 and the transcription factor twist in Sezary syndrome identified by gene expression analysis","volume":"64","author":"van Doorn","year":"2004","unstructured":"van Doorn, R. et al. Aberrant expression of the tyrosine kinase receptor EphA4 and the transcription factor twist in Sezary syndrome identified by gene expression analysis. Cancer Research. 64, 5578-5586 (2004).","journal-title":"Cancer Research"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/sj.leu.2405044","type":"journal-article","article-title":"Sezary syndrome is a unique cutaneous T-cell lymphoma as identified by an expanded gene signature including diagnostic marker molecules CDO1 and DNM3","volume":"22","author":"Booken","year":"2008","unstructured":"Booken, N. et al. Sezary syndrome is a unique cutaneous T-cell lymphoma as identified by an expanded gene signature including diagnostic marker molecules CDO1 and DNM3. Leukemia. 22, 393-399 (2008).","journal-title":"Leukemia"},{"key":"ref28","first-page":"1","type":"journal-article","article-title":"Collection , storage, and preparation of human blood cells","volume":"73","author":"Dagur","year":"2016","unstructured":"Dagur, P.K., McCoy, Jr., J.P. Collection, storage, and preparation of human blood cells. Current Protocol Cytometry. 73, 5.1.1-5.1.16 (2016).","journal-title":"Current Protocol Cytometry"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1159\/000112928","type":"journal-article","article-title":"Heterogeneity of Circulating CD4+ Memory T-cell Subsets in Erythrodermic Patients: CD27 Analysis Can Help to Distinguish Cutaneous T-cell Lymphomas From Inflammatory Erythroderma","volume":"216","author":"Fierro","year":"2008","unstructured":"Fierro, M.T. et al. Heterogeneity of Circulating CD4+ Memory T-cell Subsets in Erythrodermic Patients: CD27 Analysis Can Help to Distinguish Cutaneous T-cell Lymphomas From Inflammatory Erythroderma. Dermatology. 216 (3), 213-221 (2008).","journal-title":"Dermatology"},{"issue":"5","key":"ref30","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1182\/blood-2009-11-251926","type":"journal-article","article-title":"S\u00e9zary syndrome and mycosis fungoides arise from distinct T-cell subsets: a biologic rationale for their distinct clinical behaviors","volume":"116","author":"Campbell","year":"2010","unstructured":"Campbell, J.J. et al. S\u00e9zary syndrome and mycosis fungoides arise from distinct T-cell subsets: a biologic rationale for their distinct clinical behaviors. Blood. 116 (5), 767-771 (2010).","journal-title":"Blood"},{"issue":"12","key":"ref31","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1182\/blood-2017-03-772996","type":"journal-article","article-title":"Circulating and skin-derived Sezary cells: clonal but with phenotypic plasticity","volume":"130","author":"Roelens","year":"2017","unstructured":"Roelens, M. et al. Circulating and skin-derived S \u00b4ezary cells: clonal but with phenotypic plasticity. Blood. 130 (12), 1468-1471 (2017).","journal-title":"Blood"},{"issue":"18","key":"ref32","doi-asserted-by":"crossref","first-page":"11796","DOI":"10.1073\/pnas.092284399","type":"journal-article","article-title":"Genomic expression programs and the integration of the CD28 costimulatory signal in T cell activation","volume":"99","author":"Diehn","year":"2002","unstructured":"Diehn, M. et al. Genomic expression programs and the integration of the CD28 costimulatory signal in T cell activation. Proceedings of the National Academy of Sciences of the United States of America. 99 (18), 11796-11801 (2002).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref33","first-page":"12","type":"journal-article","article-title":"New therapies and immunological findings in cutaneous T-cell lymphoma","volume":"8","author":"Fuji","year":"2018","unstructured":"Fuji, K. New therapies and immunological findings in cutaneous T-cell lymphoma. Frontiers in Oncology. 8, 12-28 (2018).","journal-title":"Frontiers in Oncology"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61470\/isolating-human-peripheral-blood-mononuclear-cells-cd4-t-cells-from","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:51:33Z","timestamp":1792615893000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,14]]},"references-count":33,"journal-issue":{"issue":"176","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/61470","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,14]]}}}