{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:38:24Z","timestamp":1792625904343,"version":"4.5.0"},"reference-count":39,"publisher":"MyJove Corporation","issue":"160","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61522","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T20:00:20Z","timestamp":1593028820000},"source":"Crossref","is-referenced-by-count":13,"title":["Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration"],"prefix":"10.3791","author":[{"given":"Stephanie","family":"Tsai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2020,6,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.5962\/bhl.title.158642","type":"book","volume-title":"Prodromo Di Un'opera Da Imprimersi Sopra Le Riproduzioni Animali","author":"Spallanzani","year":"1768","unstructured":"Spallanzani, L. Prodromo Di Un'opera Da Imprimersi Sopra Le Riproduzioni Animali. Nella Stamperia di Giovanni Montanari, 1768, (1768)."},{"key":"ref2","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaq0681","type":"journal-article","article-title":"Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration","author":"Gerber","year":"2018","unstructured":"Gerber, T. et al. Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Science. (2018).","journal-title":"Science"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"5153","DOI":"10.1038\/s41467-018-07604-0","type":"journal-article","article-title":"Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution","volume":"9","author":"Leigh","year":"2018","unstructured":"Leigh, N. D. et al. Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution. Nature Communications. 9 (1), 5153 (2018).","journal-title":"Nature Communications"},{"issue":"7251","key":"ref4","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/nature08152","type":"journal-article","article-title":"Cells keep a memory of their tissue origin during axolotl limb regeneration","volume":"460","author":"Kragl","year":"2009","unstructured":"Kragl, M. et al. Cells keep a memory of their tissue origin during axolotl limb regeneration. Nature. 460 (7251), 60-65 (2009).","journal-title":"Nature"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/reg2.32","type":"journal-article","article-title":"The axolotl limb blastema: cellular and molecular mechanisms driving blastema formation and limb regeneration in tetrapods","volume":"2","author":"McCusker","year":"2015","unstructured":"McCusker, C., Bryant, S. V., Gardiner, D. M. The axolotl limb blastema: cellular and molecular mechanisms driving blastema formation and limb regeneration in tetrapods. Regeneration (Oxford). 2 (2), 54-71 (2015).","journal-title":"Regeneration (Oxford)"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ydbio.2004.02.016","type":"journal-article","article-title":"A stepwise model system for limb regeneration","volume":"270","author":"Endo","year":"2004","unstructured":"Endo, T., Bryant, S. V., Gardiner, D. M. A stepwise model system for limb regeneration. Developmental Biology. 270 (1), 135-145 (2004).","journal-title":"Developmental Biology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"100024","DOI":"10.1016\/j.regen.2019.100024","type":"journal-article","article-title":"The molecular interplay between progenitors and immune cells in tissue regeneration and homeostasis","volume":"7","author":"Tsai","year":"2020","unstructured":"Tsai, S. L. The molecular interplay between progenitors and immune cells in tissue regeneration and homeostasis. Journal of Immunology and Regenerative Medicine. 7 100024 (2020).","journal-title":"Journal of Immunology and Regenerative Medicine"},{"issue":"23","key":"ref8","doi-asserted-by":"crossref","first-page":"9415","DOI":"10.1073\/pnas.1300290110","type":"journal-article","article-title":"Macrophages are required for adult salamander limb regeneration","volume":"110","author":"Godwin","year":"2013","unstructured":"Godwin, J. W., Pinto, A. R., Rosenthal, N. A. Macrophages are required for adult salamander limb regeneration. Proceedings of the National Academy of Sciences of the United States of America. 110 (23), 9415-9420 (2013).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"7","key":"ref9","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1016\/j.cell.2016.05.038","type":"journal-article","article-title":"The molecular and cellular choreography of appendage regeneration","volume":"165","author":"Tanaka","year":"2016","unstructured":"Tanaka, E. M. The molecular and cellular choreography of appendage regeneration. Cell. 165 (7), 1598-1608 (2016).","journal-title":"Cell"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s00018-007-7433-z","type":"journal-article","article-title":"Wound epidermis formation and function in urodele amphibian limb regeneration","volume":"65","author":"Campbell","year":"2008","unstructured":"Campbell, L. J., Crews, C. M. Wound epidermis formation and function in urodele amphibian limb regeneration. Cellular and Molecular Life Sciences. 65 (1), 73-79 (2008).","journal-title":"Cellular and Molecular Life Sciences"},{"issue":"47","key":"ref11","doi-asserted-by":"crossref","first-page":"12501","DOI":"10.1073\/pnas.1706855114","type":"journal-article","article-title":"Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration","volume":"114","author":"Fei","year":"2017","unstructured":"Fei, J. F. et al. Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration. Proceedings of the National Academy of Sciences of the United States of America. 114 (47), 12501-12506 (2017).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.stem.2013.11.007","type":"journal-article","article-title":"Fundamental differences in dedifferentiation and stem cell recruitment during skeletal muscle regeneration in two salamander species","volume":"14","author":"Sandoval-Guzman","year":"2014","unstructured":"Sandoval-Guzman, T. et al. Fundamental differences in dedifferentiation and stem cell recruitment during skeletal muscle regeneration in two salamander species. Cell Stem Cell. 14 (2), 174-187 (2014).","journal-title":"Cell Stem Cell"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1432-0436.1975.tb01439.x","type":"journal-article","article-title":"The roles of injury, nerves, and the wound epidermis during the initiation of amphibian limb regeneration","volume":"4","author":"Tassava","year":"1975","unstructured":"Tassava, R. A., Mescher, A. L. The roles of injury, nerves, and the wound epidermis during the initiation of amphibian limb regeneration. Differentiation. 4 (1), 23-24 (1975).","journal-title":"Differentiation"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/0012-1606(61)90009-4","type":"journal-article","article-title":"Origin of the blastema in regenerating limbs of the newt Triturus viridescens. An autoradiographic study using tritiated thymidine to follow cell proliferation and migration","volume":"3","author":"Hay","year":"1961","unstructured":"Hay, E. D., Fischman, D. A. Origin of the blastema in regenerating limbs of the newt Triturus viridescens. An autoradiographic study using tritiated thymidine to follow cell proliferation and migration. Developmental Biology. 3 26-59 (1961).","journal-title":"Developmental Biology"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1002\/(SICI)1097-0177(200002)217:2<216::AID-DVDY8>3.0.CO;2-8","type":"journal-article","article-title":"Apical epithelial cap morphology and fibronectin gene expression in regenerating axolotl limbs","volume":"217","author":"Christensen","year":"2000","unstructured":"Christensen, R. N., Tassava, R. A. Apical epithelial cap morphology and fibronectin gene expression in regenerating axolotl limbs. Developmental Dynamics. 217 (2), 216-224 (2000).","journal-title":"Developmental Dynamics"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1002\/aja.1001510408","type":"journal-article","article-title":"Scanning electron microscopy of epidermal cell migration in wound healing during limb regeneration in the adult newt, Notophthalmus viridescens","volume":"151","author":"Repesh","year":"1978","unstructured":"Repesh, L. A., Oberpriller, J. C. Scanning electron microscopy of epidermal cell migration in wound healing during limb regeneration in the adult newt, Notophthalmus viridescens. American Journal of Anatomy. 151 (4), 539-555 (1978).","journal-title":"American Journal of Anatomy"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1002\/(SICI)1097-0185(199605)245:1<122::AID-AR17>3.0.CO;2-R","type":"journal-article","article-title":"Stabilizing role of the basement membrane and dermal fibers during newt limb regeneration","volume":"245","author":"Neufeld","year":"1996","unstructured":"Neufeld, D. A., Day, F. A., Settles, H. E. Stabilizing role of the basement membrane and dermal fibers during newt limb regeneration. Anatomical Record. 245 (1), 122-127 (1996).","journal-title":"Anatomical Record"},{"key":"ref18","type":"book","volume-title":"Growth in Living Systems","author":"Singer","year":"1961","unstructured":"Singer, M., Saltpeter, M. M. in Growth in Living Systems (ed M.X. Zarrow) Basic Books, New York. (1961)."},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/jez.1401950111","type":"journal-article","article-title":"Effects on adult newt limb regeneration of partial and complete skin flaps over the amputation surface","volume":"195","author":"Mescher","year":"1976","unstructured":"Mescher, A. L. Effects on adult newt limb regeneration of partial and complete skin flaps over the amputation surface. Journal of Experimental Zoology. 195 (1), 117-128 (1976).","journal-title":"Journal of Experimental Zoology"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/jez.1402080111","type":"journal-article","article-title":"Regenerative responses in larval axolotl limbs with skin grafts over the amputation surface","volume":"208","author":"Tassava","year":"1979","unstructured":"Tassava, R. A., Garling, D. J. Regenerative responses in larval axolotl limbs with skin grafts over the amputation surface. Journal of Experimental Zoology. 208 (1), 97-110 (1979).","journal-title":"Journal of Experimental Zoology"},{"key":"ref21","first-page":"348","type":"journal-article","article-title":"Der Kampf der Gewebe im Regeneratbei Begunsiigung der Hautregeneralion","volume":"22","author":"Tornier","year":"1906","unstructured":"Tornier, G. Der Kampf der Gewebe im Regeneratbei Begunsiigung der Hautregeneralion. Arch. Entwmech. 22 348-352 (1906).","journal-title":"Arch. Entwmech"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/ar.1091260103","type":"journal-article","article-title":"Regenerative inhibition following limb amputation and immediate insertion into the body cavity","volume":"126","author":"Goss","year":"1956","unstructured":"Goss, R. J. Regenerative inhibition following limb amputation and immediate insertion into the body cavity. Anatomical Record. 126 (1), 15-27 (1956).","journal-title":"Anatomical Record"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/jez.1401340209","type":"journal-article","article-title":"The effect of apical cap removal on limb regeneration in Amblystoma larvae","volume":"134","author":"Thornton","year":"1957","unstructured":"Thornton, C. S. The effect of apical cap removal on limb regeneration in Amblystoma larvae. Journal of Experimental Zoology. 134 (2), 357-381 (1957).","journal-title":"Journal of Experimental Zoology"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/jez.1401370108","type":"journal-article","article-title":"The inhibition of limb regeneration in urodele larvae by localized irradiation with ultraviolet light","volume":"137","author":"Thornton","year":"1958","unstructured":"Thornton, C. S. The inhibition of limb regeneration in urodele larvae by localized irradiation with ultraviolet light. Journal of Experimental Zoology. 137 (1), 153-179 (1958).","journal-title":"Journal of Experimental Zoology"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.50765","type":"journal-article","article-title":"Midkine is a dual regulator of wound epidermis development and inflammation during the initiation of limb regeneration","volume":"9","author":"Tsai","year":"2020","unstructured":"Tsai, S. L., Baselga-Garriga, C., Melton, D. A. Midkine is a dual regulator of wound epidermis development and inflammation during the initiation of limb regeneration. Elife. 9 (2020).","journal-title":"Elife"},{"issue":"7690","key":"ref26","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature25458","type":"journal-article","article-title":"The axolotl genome and the evolution of key tissue formation regulators","volume":"554","author":"Nowoshilow","year":"2018","unstructured":"Nowoshilow, S. et al. The axolotl genome and the evolution of key tissue formation regulators. Nature. 554 (7690), 50-55 (2018).","journal-title":"Nature"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"2286","DOI":"10.1038\/s41467-017-01964-9","type":"journal-article","article-title":"Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration","volume":"8","author":"Elewa","year":"2017","unstructured":"Elewa, A. et al. Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration. Nature Communications. 8 (1), 2286 (2017).","journal-title":"Nature Communications"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.celrep.2016.12.063","type":"journal-article","article-title":"A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors","volume":"18","author":"Bryant","year":"2017","unstructured":"Bryant, D. M. et al. A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors. Cell Reports. 18 (3), 762-776 (2017).","journal-title":"Cell Reports"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/gb-2013-14-2-r16","type":"journal-article","article-title":"A de novo assembly of the newt transcriptome combined with proteomic validation identifies new protein families expressed during tissue regeneration","volume":"14","author":"Looso","year":"2013","unstructured":"Looso, M. et al. A de novo assembly of the newt transcriptome combined with proteomic validation identifies new protein families expressed during tissue regeneration. Genome Biology. 14 (2), R16 (2013).","journal-title":"Genome Biology"},{"issue":"8","key":"ref30","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.yexcr.2013.02.013","type":"journal-article","article-title":"A reference transcriptome and inferred proteome for the salamander Notophthalmus viridescens","volume":"319","author":"Abdullayev","year":"2013","unstructured":"Abdullayev, I., Kirkham, M., Bjorklund, A. K., Simon, A., Sandberg, R. A reference transcriptome and inferred proteome for the salamander Notophthalmus viridescens. Experimental Cell Research. 319 (8), 1187-1197 (2013).","journal-title":"Experimental Cell Research"},{"key":"ref31","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.22054","type":"journal-article","article-title":"Transcriptome analysis illuminates the nature of the intracellular interaction in a vertebrate-algal symbiosis","volume":"6","author":"Burns","year":"2017","unstructured":"Burns, J. A., Zhang, H., Hill, E., Kim, E., Kerney, R. Transcriptome analysis illuminates the nature of the intracellular interaction in a vertebrate-algal symbiosis. Elife. 6 (2017).","journal-title":"Elife"},{"issue":"10","key":"ref32","doi-asserted-by":"crossref","first-page":"109831","DOI":"10.1371\/journal.pone.0109831","type":"journal-article","article-title":"A transcriptome for the study of early processes of retinal regeneration in the adult newt, Cynops pyrrhogaster","volume":"9","author":"Nakamura","year":"2014","unstructured":"Nakamura, K. et al. A transcriptome for the study of early processes of retinal regeneration in the adult newt, Cynops pyrrhogaster. PLoS One. 9 (10), e109831 (2014).","journal-title":"PLoS One"},{"issue":"2","key":"ref33","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1101\/gr.241901.118","type":"journal-article","article-title":"A chromosome-scale assembly of the axolotl genome","volume":"29","author":"Smith","year":"2019","unstructured":"Smith, J. J. et al. A chromosome-scale assembly of the axolotl genome. Genome Research. 29 (2), 317-324 (2019).","journal-title":"Genome Research"},{"issue":"704","key":"ref34","type":"journal-article","article-title":"Using transcriptomics to enable a plethodontid salamander (Bolitoglossa ramosi) for limb regeneration research","volume":"19","author":"Arenas Gomez","year":"2018","unstructured":"Arenas Gomez, C. M., Woodcock, R. M., Smith, J. J., Voss, S. R., Delgado, J. P. Using transcriptomics to enable a plethodontid salamander (Bolitoglossa ramosi) for limb regeneration research. BMC Genomics. 19 (704) (2018).","journal-title":"BMC Genomics"},{"issue":"12","key":"ref35","doi-asserted-by":"crossref","first-page":"2908","DOI":"10.1038\/s41596-018-0071-0","type":"journal-article","article-title":"Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum)","volume":"13","author":"Fei","year":"2018","unstructured":"Fei, J. F. et al. Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum). Nature Protocols. 13 (12), 2908-2943 (2018).","journal-title":"Nature Protocols"},{"issue":"10","key":"ref36","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1242\/dev.105072","type":"journal-article","article-title":"Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease","volume":"141","author":"Flowers","year":"2014","unstructured":"Flowers, G. P., Timberlake, A. T., McLean, K. C., Monaghan, J. R., Crews, C. M. Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease. Development. 141 (10), 2165-2171 (2014).","journal-title":"Development"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/nprot.2014.040","type":"journal-article","article-title":"Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination","volume":"9","author":"Khattak","year":"2014","unstructured":"Khattak, S. et al. Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination. Nature Protocols. 9 (3), 529-540 (2014).","journal-title":"Nature Protocols"},{"issue":"14","key":"ref38","doi-asserted-by":"crossref","DOI":"10.1242\/dev.167700","type":"journal-article","article-title":"Model systems for regeneration: salamanders","volume":"146","author":"Joven","year":"2019","unstructured":"Joven, A., Elewa, A., Simon, A. Model systems for regeneration: salamanders. Development. 146 (14) (2019).","journal-title":"Development"},{"issue":"2","key":"ref39","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.ydbio.2017.07.010","type":"journal-article","article-title":"Systemic cell cycle activation is induced following complex tissue injury in axolotl","volume":"433","author":"Johnson","year":"2018","unstructured":"Johnson, K., Bateman, J., DiTommaso, T., Wong, A. Y., Whited, J. L. Systemic cell cycle activation is induced following complex tissue injury in axolotl. Developmental Biology. 433 (2), 461-472 (2018).","journal-title":"Developmental Biology"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61522\/inhibition-wound-epidermis-formation-via-full-skin-flap-surgery","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:52:44Z","timestamp":1792623164000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,24]]},"references-count":39,"journal-issue":{"issue":"160","published-online":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.3791\/61522","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,24]]}}}