{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:40:05Z","timestamp":1792622405895,"version":"4.5.0"},"reference-count":32,"publisher":"MyJove Corporation","issue":"167","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61808","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T19:00:08Z","timestamp":1611774008000},"source":"Crossref","is-referenced-by-count":3,"title":["A non-coding small RNA MicC contributes to virulence in outer membrane proteins in Salmonella Enteritidis"],"prefix":"10.3791","author":[{"given":"Xia","family":"Meng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianchen","family":"Meng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianye","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinqiu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,1,27]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"194504","DOI":"10.1016\/j.bbagrm.2020.194504","type":"journal-article","article-title":"sRNA-mediated control in bacteria: An increasing diversity of regulatory mechanisms","volume":"1863","author":"J\u00f8rgensen","year":"2020","unstructured":"J\u00f8rgensen, M. G., Pettersen, J. S., Kallipolitis, B. H. sRNA-mediated control in bacteria: An increasing diversity of regulatory mechanisms. Biochimica et Biophysica Acta-Gene Regulatory Mechanisms. 1863 (5), 194504 (2020).","journal-title":"Biochimica et Biophysica Acta-Gene Regulatory Mechanisms"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/bs.adgen.2015.05.001","type":"journal-article","article-title":"Small RNAs in bacteria and archaea: who they are, what they do, and how they do it","volume":"90","author":"Wagner","year":"2015","unstructured":"Wagner, E. G. H., Romby, P. Small RNAs in bacteria and archaea: who they are, what they do, and how they do it. Advances In Genetics. 90, 133-208 (2015).","journal-title":"Advances In Genetics"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2958.2008.06505.x","type":"journal-article","article-title":"A rough guide to the non-coding RNA world of Salmonella","volume":"71","author":"Vogel","year":"2009","unstructured":"Vogel, J. A rough guide to the non-coding RNA world of Salmonella. Molecular Microbiology. 71 (1), 1-11 (2009).","journal-title":"Molecular Microbiology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.gene.2018.02.068","type":"journal-article","article-title":"Small RNA-mediated regulation in bacteria: A growing palette of diverse mechanisms","volume":"656","author":"Dutta","year":"2018","unstructured":"Dutta, T., Srivastava, S. Small RNA-mediated regulation in bacteria: A growing palette of diverse mechanisms. Gene. 656, 60-72 (2018).","journal-title":"Gene"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.cell.2009.01.043","type":"journal-article","article-title":"Regulatory RNAs in bacteria","volume":"136","author":"Waters","year":"2009","unstructured":"Waters, L. S., Storz, G. Regulatory RNAs in bacteria. Cell. 136 (4), 615-628 (2009).","journal-title":"Cell"},{"issue":"20","key":"ref6","doi-asserted-by":"crossref","first-page":"6689","DOI":"10.1128\/JB.186.20.6689-6697.2004","type":"journal-article","article-title":"MicC, a second small-RNA regulator of Omp protein expression in Escherichia coli","volume":"186","author":"Chen","year":"2004","unstructured":"Chen, S., Zhang, A., Blyn, L. B., Storz, G. MicC, a second small-RNA regulator of Omp protein expression in Escherichia coli. Journal of Bacteriology. 186 (20), 6689-6697 (2004).","journal-title":"Journal of Bacteriology"},{"issue":"8","key":"ref7","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1038\/nsmb.1631","type":"journal-article","article-title":"Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation","volume":"16","author":"Pfeiffer","year":"2009","unstructured":"Pfeiffer, V., Papenfort, K., Lucchini, S., Hinton, J. C., Vogel, J. Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation. Nature Structural & Molecular Biology. 16 (8), 840-846 (2009).","journal-title":"Nature Structural & Molecular Biology"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3390\/antibiotics6040033","type":"journal-article","article-title":"Dual Regulation of the Small RNA MicC and the Quiescent Porin OmpN in Response to Antibiotic Stress in Escherichia coli","volume":"6","author":"Dam","year":"2017","unstructured":"Dam, S., Pag\u00e8s, J. M., Masi, M. Dual Regulation of the Small RNA MicC and the Quiescent Porin OmpN in Response to Antibiotic Stress in Escherichia coli. Antibiotics (Basel). 6 (4), 33 (2017).","journal-title":"Antibiotics (Basel)"},{"issue":"9","key":"ref9","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1089\/mdr.2017.0379","type":"journal-article","article-title":"Porin Deficiency in Carbapenem-Resistant Enterobacter aerogenes Strains","volume":"24","author":"Hao","year":"2018","unstructured":"Hao, M. et al. Porin Deficiency in Carbapenem-Resistant Enterobacter aerogenes Strains. Microbial Drug Resistance. 24 (9), 1277-1283 (2018).","journal-title":"Microbial Drug Resistance"},{"issue":"7","key":"ref10","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1261\/rna.055251.115","type":"journal-article","article-title":"Hfq assists small RNAs in binding to the coding sequence of ompD mRNA and in rearranging its structure","volume":"22","author":"Wroblewska","year":"2016","unstructured":"Wroblewska, Z., Olejniczak, M. Hfq assists small RNAs in binding to the coding sequence of ompD mRNA and in rearranging its structure. RNA. 22 (7), 979-994 (2016).","journal-title":"RNA"},{"issue":"19","key":"ref11","doi-asserted-by":"crossref","first-page":"5901","DOI":"10.1128\/JB.185.19.5901-5905.2003","type":"journal-article","article-title":"Global regulation of the Salmonella enterica serovar typhimurium major porin, OmpD","volume":"185","author":"Santiviago","year":"2003","unstructured":"Santiviago, C. A., Toro, C. S., Hidalgo, A. A., Youderian, P., Mora, G. C. Global regulation of the Salmonella enterica serovar typhimurium major porin, OmpD. Journal of Bacteriology. 185 (19), 5901-5905 (2003).","journal-title":"Journal of Bacteriology"},{"issue":"9","key":"ref12","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1139\/w04-056","type":"journal-article","article-title":"OmpD but not OmpC is involved in adherence of Salmonella enterica serovar typhimurium to human cells","volume":"50","author":"Hara-Kaonga","year":"2004","unstructured":"Hara-Kaonga, B., Pistole, T. G. OmpD but not OmpC is involved in adherence of Salmonella enterica serovar typhimurium to human cells. Canadian Journal of Microbiology. 50 (9), 719-727 (2004).","journal-title":"Canadian Journal of Microbiology"},{"issue":"12","key":"ref13","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","type":"journal-article","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","unstructured":"Datsenko, K. A., Wanner, B. L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proceedings of the National Academy of Sciences of the United States of America. 97 (12), 6640-6645 (2000).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/1574-6968.12206","type":"journal-article","article-title":"The RNA chaperone Hfq regulates expression of fimbrial-related genes and virulence of Salmonella enterica serovar Enteritidis","volume":"346","author":"Meng","year":"2013","unstructured":"Meng, X. et al. The RNA chaperone Hfq regulates expression of fimbrial-related genes and virulence of Salmonella enterica serovar Enteritidis. FEMS Microbiology Letters. 346 (2), 90-96 (2013).","journal-title":"FEMS Microbiology Letters"},{"issue":"4","key":"ref15","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","type":"journal-article","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","unstructured":"Livak, K. J., Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25 (4), 402-408 (2001).","journal-title":"Methods"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.1128\/IAI.66.6.2803-2808.1998","type":"journal-article","article-title":"Multiple fimbrial adhesins are required for full virulence of Salmonella typhimurium in mice","volume":"66","author":"Vander Velden","year":"1998","unstructured":"Van der Velden, A. W., B\u00e4umler, A. J., Tsolis, R. M., Heffron, F. Multiple fimbrial adhesins are required for full virulence of Salmonella typhimurium in mice. Infection and Immunity. 66 (6), 2803-2808 (1998).","journal-title":"Infection and Immunity"},{"issue":"10","key":"ref17","first-page":"736","type":"journal-article","article-title":"Salmonella enterica serovar enteritidis in humans and animals: Epidemiology, pathogenesis, and control","volume":"40","author":"Prescott","year":"1999","unstructured":"Prescott, J. F. Salmonella enterica serovar enteritidis in humans and animals: Epidemiology, pathogenesis, and control. Canadian Veterinary Journal La Revue Veterinaire Canadienne. 40 (10), 736 (1999).","journal-title":"Canadian Veterinary Journal La Revue Veterinaire Canadienne"},{"issue":"6","key":"ref18","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1080\/21645515.2018.1504717","type":"journal-article","article-title":"The global burden and epidemiology of invasive non-typhoidal","volume":"15","author":"Balasubramanian","year":"2019","unstructured":"Balasubramanian, R. et al. The global burden and epidemiology of invasive non-typhoidal Salmonella infections. Hum Vaccin Immunother. 15 (6), 1421-1426 (2019).","journal-title":"Hum Vaccin Immunother"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1365-2672.2004.02294.x","type":"journal-article","article-title":"Colonization of the chicken reproductive tract and egg contamination by Salmonella","volume":"97","author":"De Buck","year":"2004","unstructured":"De Buck, J., Van Immerseel, F., Haesebrouck, F., Ducatelle, R. Colonization of the chicken reproductive tract and egg contamination by Salmonella. Journal of General and Applied Microbiology. 97 (2), 233-245 (2004).","journal-title":"Journal of General and Applied Microbiology"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1093\/nar\/gkn050","type":"journal-article","article-title":"Small RNAs encoded within genetic islands of Salmonella typhimurium show host-induced expression and role in virulence","volume":"36","author":"Padalon-Brauch","year":"2008","unstructured":"Padalon-Brauch, G. et al. Small RNAs encoded within genetic islands of Salmonella typhimurium show host-induced expression and role in virulence. Nucleic Acids Research. 36 (6), 1913-1927 (2008).","journal-title":"Nucleic Acids Research"},{"issue":"7","key":"ref21","doi-asserted-by":"crossref","first-page":"1000477","DOI":"10.1371\/journal.ppat.1000477","type":"journal-article","article-title":"Analysis of pools of targeted Salmonella deletion mutants identifies novel genes affecting fitness during competitive infection in mice","volume":"5","author":"Santiviago","year":"2009","unstructured":"Santiviago, C. A. et al. Analysis of pools of targeted Salmonella deletion mutants identifies novel genes affecting fitness during competitive infection in mice. PLoS Pathogens. 5 (7), e1000477 (2009).","journal-title":"PLoS Pathogens"},{"issue":"9","key":"ref22","doi-asserted-by":"crossref","first-page":"1002120","DOI":"10.1371\/journal.ppat.1002120","type":"journal-article","article-title":"A Salmonella small non-coding RNA facilitates bacterial invasion and intracellular replication by modulating the expression of virulence factors","volume":"7","author":"Gong","year":"2011","unstructured":"Gong, H. et al. A Salmonella small non-coding RNA facilitates bacterial invasion and intracellular replication by modulating the expression of virulence factors. PLoS Pathogens. 7 (9), e1002120 (2011).","journal-title":"PLoS Pathogens"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"437","DOI":"10.4161\/rna.20480","type":"journal-article","article-title":"sRNAs and the virulence of Salmonella enterica serovar Typhimurium","volume":"9","author":"H\u00e9brard","year":"2012","unstructured":"H\u00e9brard, M. et al. sRNAs and the virulence of Salmonella enterica serovar Typhimurium. RNA Biology. 9 (4), 437-445 (2012).","journal-title":"RNA Biology"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.mib.2006.10.006","type":"journal-article","article-title":"Small non-coding RNAs and the bacterial outer membrane","volume":"9","author":"Vogel","year":"2006","unstructured":"Vogel, J., Papenfort, K. Small non-coding RNAs and the bacterial outer membrane. Current Opinion in Microbiology. 9 (6), 605-611 (2006).","journal-title":"Current Opinion in Microbiology"},{"issue":"6","key":"ref25","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1111\/j.1365-2958.2006.05524.x","type":"journal-article","article-title":"SigmaE-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay","volume":"62","author":"Papenfort","year":"2006","unstructured":"Papenfort, K. et al. SigmaE-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay. Molecular Microbiology. 62 (6), 1674-1688 (2006).","journal-title":"Molecular Microbiology"},{"issue":"19","key":"ref26","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1101\/gad.354405","type":"journal-article","article-title":"Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA","volume":"19","author":"Udekwu","year":"2005","unstructured":"Udekwu, K. I. et al. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes and Development. 19 (19), 2355-2366 (2005).","journal-title":"Genes and Development"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.resmic.2009.03.004","type":"journal-article","article-title":"Multiple target regulation by small noncoding RNAs rewires gene expression at the post-transcriptional level","volume":"160","author":"Papenfort","year":"2009","unstructured":"Papenfort, K., Vogel, J. Multiple target regulation by small noncoding RNAs rewires gene expression at the post-transcriptional level. Research in Microbiology. 160 (4), 278-287 (2009).","journal-title":"Research in Microbiology"},{"issue":"21","key":"ref28","doi-asserted-by":"crossref","first-page":"12456","DOI":"10.1073\/pnas.95.21.12456","type":"journal-article","article-title":"Riboregulation in Escherichia coli: DsrA RNA acts by RNA:RNA interactions at multiple loci","volume":"95","author":"Lease","year":"1998","unstructured":"Lease, R. A., Cusick, M. E., Belfort, M. Riboregulation in Escherichia coli: DsrA RNA acts by RNA:RNA interactions at multiple loci. Proceedings of the National Academy of Sciences of the United States of America. 95 (21), 12456-12461 (1998).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"21","key":"ref29","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.1101\/gad.447207","type":"journal-article","article-title":"A small RNA regulates multiple ABC transporter mRNAs by targeting C\/A-rich elements inside and upstream of ribosome-binding sites","volume":"21","author":"Sharma","year":"2007","unstructured":"Sharma, C. M., Darfeuille, F., Plantinga, T. H., Vogel, J. A small RNA regulates multiple ABC transporter mRNAs by targeting C\/A-rich elements inside and upstream of ribosome-binding sites. Genes and Development. 21 (21), 2804-2817 (2007).","journal-title":"Genes and Development"},{"issue":"11","key":"ref30","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1101\/gad.423507","type":"journal-article","article-title":"Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism","volume":"21","author":"Boisset","year":"2007","unstructured":"Boisset, S. et al. Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism. Genes and Development. 21 (11), 1353-1366 (2007).","journal-title":"Genes and Development"},{"issue":"20","key":"ref31","doi-asserted-by":"crossref","first-page":"6962","DOI":"10.1128\/JB.187.20.6962-6971.2005","type":"journal-article","article-title":"Effect of RyhB small RNA on global iron use in Escherichia coli","volume":"187","author":"Mass\u00e9","year":"2005","unstructured":"Mass\u00e9, E., Vanderpool, C. K., Gottesman, S. Effect of RyhB small RNA on global iron use in Escherichia coli. Journal of Bacteriology. 187 (20), 6962-6971 (2005).","journal-title":"Journal of Bacteriology"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.chom.2010.06.008","type":"journal-article","article-title":"Regulatory RNA in bacterial pathogens","volume":"8","author":"Papenfort","year":"2010","unstructured":"Papenfort, K., Vogel, J. Regulatory RNA in bacterial pathogens. Cell Host & Microbe. 8 (1), 116-127 (2010).","journal-title":"Cell Host & Microbe"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61808\/a-non-coding-small-rna-micc-contributes-to-virulence-outer-membrane","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:08:24Z","timestamp":1792620504000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,27]]},"references-count":32,"journal-issue":{"issue":"167","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/61808","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,27]]}}}