{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:40:54Z","timestamp":1792618854820,"version":"4.5.0"},"reference-count":10,"publisher":"MyJove Corporation","issue":"171","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/61854","type":"journal-article","created":{"date-parts":[[2021,5,30]],"date-time":"2021-05-30T20:00:33Z","timestamp":1622404833000},"source":"Crossref","is-referenced-by-count":1,"title":["Decellularization of the Murine Cardiopulmonary Complex"],"prefix":"10.3791","author":[{"given":"Alejandro E.","family":"Mayorca-Guiliani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Rafaeva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Willacy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris D.","family":"Madsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Reuten","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine  T.","family":"Erler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,5,30]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1126\/science.1176009","type":"journal-article","article-title":"Extracellular matrix: not just pretty fibrils","volume":"326","author":"Hynes","year":"2009","unstructured":"Hynes, R.O. Extracellular matrix: not just pretty fibrils. Science. 326, 1216-1219 (2009).","journal-title":"Science"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1038\/nm.4352","type":"journal-article","article-title":"ISDoT: in situ decellularization of tissues for high-resolution imaging and proteomic analysis of native extracellular matrix","volume":"23","author":"Mayorca-Guiliani","year":"2017","unstructured":"Mayorca-Guiliani, A. E.et al. ISDoT: in situ decellularization of tissues for high-resolution imaging and proteomic analysis of native extracellular matrix. Nature Medicine. 23, 890-898 (2017).","journal-title":"Nature Medicine"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1038\/s41596-019-0225-8","type":"journal-article","article-title":"Decellularization and antibody staining of mouse tissues to map native extracellular matrix structures in 3D","volume":"14","author":"Mayorca-Guiliani","year":"2019","unstructured":"Mayorca-Guiliani, A. E. et al. Decellularization and antibody staining of mouse tissues to map native extracellular matrix structures in 3D. Nature Protocols. 14, 3395-3425 (2019).","journal-title":"Nature Protocols"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.actbio.2016.12.033","type":"journal-article","article-title":"The impact of detergents on the tissue decellularization process: A TOF-sims study","volume":"50","author":"White","year":"2017","unstructured":"White, L.J. et al. The impact of detergents on the tissue decellularization process: A TOF-sims study. ActaBiomaterialia. 50, 207-219 (2017).","journal-title":"Acta Biomaterialia"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nm1684","type":"journal-article","article-title":"Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart","volume":"14","author":"Ott","year":"2008","unstructured":"Ott, H.C. et al. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nature Medicine. 14, 213-221 (2008).","journal-title":"Nature Medicine"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nm.2170","type":"journal-article","article-title":"Organ re-engineering through development of a transplantable recellularized liver graft using decellularized liver matrix","volume":"16","author":"Uygun","year":"2010","unstructured":"Uygun, B.E. et al. Organ re-engineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nature Medicine. 16, 814-820 (2010).","journal-title":"Nature Medicine"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1038\/nprot.2015.085","type":"journal-article","article-title":"Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging","volume":"10","author":"Susaki","year":"2015","unstructured":"Susaki, E. A.et al. Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging. Nature Protocols. 10, 1709-1727 (2015).","journal-title":"Nature Protocols"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1038\/nprot.2014.123","type":"journal-article","article-title":"Advanced CLARITY for rapid and high-resolution imaging of intact tissues","volume":"9","author":"Tomer","year":"2014","unstructured":"Tomer, R., Ye, L., Hsueh, B., Deisseroth, K. Advanced CLARITY for rapid and high-resolution imaging of intact tissues. Nature Protocols. 9, 1682-1697 (2014).","journal-title":"Nature Protocols"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1038\/nprot.2012.119","type":"journal-article","article-title":"Three-dimensional imaging of solvent-cleared organs using 3DISCO","volume":"7","author":"Erturk","year":"2012","unstructured":"Erturk, A.et al. Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nature Protocols. 7, 1983-1995 (2012).","journal-title":"Nature Protocols"},{"key":"ref10","type":"journal-article","article-title":"A FIJI Macro for quantifying pattern in extracellular matrix","author":"Wershof","year":"2019","unstructured":"Wershof, E. et al. A FIJI Macro for quantifying pattern in extracellular matrix. BioRxiv. Doi.org\/10.1101\/867507 (2019).","journal-title":"BioRxiv"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/61854\/decellularization-of-the-murine-cardiopulmonary-complex","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:30:58Z","timestamp":1792618258000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/61854"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,30]]},"references-count":10,"journal-issue":{"issue":"171","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/61854","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,30]]}}}