{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:38:02Z","timestamp":1792622282011,"version":"4.5.0"},"reference-count":30,"publisher":"MyJove Corporation","issue":"170","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/62040","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T20:00:27Z","timestamp":1619812827000},"source":"Crossref","is-referenced-by-count":1,"title":["Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning"],"prefix":"10.3791","author":[{"given":"Nathan","family":"Grainger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenton M.","family":"Sanders","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard T.","family":"Drumm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"issue":"5","key":"ref1","first-page":"398","type":"journal-article","article-title":"Pyeloureteral dynamics in the intact and chronically obstructed multicalyceal kidney","volume":"241","author":"Constantinou","year":"1981","unstructured":"Constantinou, C. E., Djurhuus, J. C. Pyeloureteral dynamics in the intact and chronically obstructed multicalyceal kidney. The American Journal of Physiology. 241 (5), R398-411 (1981).","journal-title":"The American Journal of Physiology"},{"issue":"5","key":"ref2","first-page":"412","type":"journal-article","article-title":"Multiple-coupled pacemaker system in renal pelvis of the unicalyceal kidney","volume":"241","author":"Constantinou","year":"1981","unstructured":"Constantinou, C. E., Yamaguchi, O. Multiple-coupled pacemaker system in renal pelvis of the unicalyceal kidney. TheAmerican Journal of Physiology. 241 (5), R412-418 (1981).","journal-title":"TheAmerican Journal of Physiology"},{"issue":"3","key":"ref3","first-page":"233","type":"journal-article","article-title":"Urodynamics of the upper urinary tract","volume":"14","author":"Constantinou","year":"1976","unstructured":"Constantinou, C. E., Hrynczuk, J. R. Urodynamics of the upper urinary tract. Investigative Urology. 14 (3), 233-240 (1976).","journal-title":"Investigative Urology"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/j.1748-1716.2011.02261.x","type":"journal-article","article-title":"On the function of the mammalian renal papilla and the peristalsis of the surrounding pelvis","volume":"202","author":"Schmidt-Nielsen","year":"2011","unstructured":"Schmidt-Nielsen, B., Schmidt-Nielsen, B. On the function of the mammalian renal papilla and the peristalsis of the surrounding pelvis. Acta Physiologica (Oxford, England). 202 (3), 379-385 (2011).","journal-title":"Acta Physiologica"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/nips.1416.2002","type":"journal-article","article-title":"The renal pelvis: machinery that concentrates urine in the papilla","volume":"18","author":"Dwyer","year":"2003","unstructured":"Dwyer, T. M., Schmidt-Nielsen, B. The renal pelvis: machinery that concentrates urine in the papilla. Physiology. 18 (1), 1-6 (2003).","journal-title":"Physiology"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"480","DOI":"10.2215\/CJN.04520413","type":"journal-article","article-title":"Control of urinary drainage and voiding","volume":"10","author":"Hill","year":"2015","unstructured":"Hill, W. G. Control of urinary drainage and voiding. Clinical Journal of the American Society of Nephrology. 10 (3), 480-492 (2015).","journal-title":"Clinical Journal of the American Society of Nephrology"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1469-445X.1999.tb00084.x","type":"journal-article","article-title":"The physiology of the mammalian urinary outflow tract","volume":"84","author":"Brading","year":"1999","unstructured":"Brading, A. F. The physiology of the mammalian urinary outflow tract. Experimental Physiology. 84 (1), 215-221 (1999).","journal-title":"Experimental Physiology"},{"key":"ref8","first-page":"129","type":"journal-article","article-title":"The musculature of the human renal calices, pelvis and upper ureter","volume":"135","author":"Dixon","year":"1982","unstructured":"Dixon, J. S., Gosling, J. A. The musculature of the human renal calices, pelvis and upper ureter. Journal of Anatomy. 135 (Pt 1), 129-137 (1982).","journal-title":"Journal of Anatomy"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1113\/jphysiol.2006.116855","type":"journal-article","article-title":"Pyeloureteric peristalsis: role of atypical smooth muscle cells and interstitial cells of Cajal-like cells as pacemakers","volume":"576","author":"Lang","year":"2006","unstructured":"Lang, R. J. et al. Pyeloureteric peristalsis: role of atypical smooth muscle cells and interstitial cells of Cajal-like cells as pacemakers. The Journal of Physiology. 576, 695-705 (2006).","journal-title":"The Journal of Physiology"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S0022-5347(05)66154-X","type":"journal-article","article-title":"Characterization of the spontaneous electrical and contractile activity of smooth muscle cells in the rat upper urinary tract","volume":"166","author":"Lang","year":"2001","unstructured":"Lang, R. J., Takano, H., Davidson, M. E., Suzuki, H., Klemm, M. F. Characterization of the spontaneous electrical and contractile activity of smooth muscle cells in the rat upper urinary tract. Journal of Urology. 166 (1), 329-334 (2001).","journal-title":"Journal of Urology"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1111\/j.1440-1681.1998.tb02357.x","type":"journal-article","article-title":"Electrical basis of peristalsis in the mammalian upper urinary tract","volume":"25","author":"Lang","year":"1998","unstructured":"Lang, R. J., Exintaris, B., Teele, M. E., Harvey, J., Klemm, M. F. Electrical basis of peristalsis in the mammalian upper urinary tract. Clinical and Experimental Pharmacology & Physiology. 25 (5), 310-321 (1998).","journal-title":"Clinical and Experimental Pharmacology & Physiology"},{"issue":"3","key":"ref12","first-page":"157","type":"journal-article","article-title":"Initiation and propagation of stimulus from the renal pelvic pacemaker in pig kidney","volume":"19","author":"Morita","year":"1981","unstructured":"Morita, T., Ishizuka, G., Tsuchida, S. Initiation and propagation of stimulus from the renal pelvic pacemaker in pig kidney. Investigative Urology. 19 (3), 157-160 (1981).","journal-title":"Investigative Urology"},{"issue":"5","key":"ref13","first-page":"307","type":"journal-article","article-title":"Initiation and propagation of canine renal pelvic peristalsis","volume":"36","author":"Tsuchida","year":"1981","unstructured":"Tsuchida, S., Morita, T., Harada, T., Kimura, Y. Initiation and propagation of canine renal pelvic peristalsis. Urologia Internationalis. 36 (5), 307-314 (1981).","journal-title":"Urologia Internationalis"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1152\/ajpregu.1989.257.4.R788","type":"journal-article","article-title":"Renal calyceal and pelvic contraction rhythms","volume":"257","author":"Yamaguchi","year":"1989","unstructured":"Yamaguchi, O. A., Constantinou, C. E. Renal calyceal and pelvic contraction rhythms. American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 257 (4), R788-R795 (1989).","journal-title":"American Journal of Physiology - Regulatory Integrative and Comparative Physiology"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/j.1469-7793.1999.0867n.x","type":"journal-article","article-title":"Identification of the cells underlying pacemaker activity in the guinea-pig upper urinary tract","volume":"519","author":"Klemm","year":"1999","unstructured":"Klemm, M. F., Exintaris, B., Lang, R. J. Identification of the cells underlying pacemaker activity in the guinea-pig upper urinary tract. Journal of Physiology. 519 (3), 867-884 (1999).","journal-title":"Journal of Physiology"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1440-1681.2009.05226.x","type":"journal-article","article-title":"Spontaneous electrical and Ca2+ signals in the mouse renal pelvis that drive pyeloureteric peristalsis","volume":"37","author":"Lang","year":"2010","unstructured":"Lang, R. J. et al. Spontaneous electrical and Ca2+ signals in the mouse renal pelvis that drive pyeloureteric peristalsis. Clinical and Experimental Pharmacology & Physiology. 37 (4), 509-515 (2010).","journal-title":"Clinical and Experimental Pharmacology & Physiology"},{"issue":"4","key":"ref17","first-page":"240","type":"journal-article","article-title":"Pacemaker process of ureteral peristalsis in multicalyceal kidneys","volume":"37","author":"Lutzeyer","year":"1982","unstructured":"Lutzeyer, W. Pacemaker process of ureteral peristalsis in multicalyceal kidneys. Urologia Internationalis. 37 (4), 240-246 (1982).","journal-title":"Urologia Internationalis"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/978-981-13-5895-1_3","type":"journal-article","article-title":"Pacemaker mechanisms driving pyeloureteric peristalsis: modulatory role of interstitial cells","volume":"1124","author":"Lang","year":"2019","unstructured":"Lang, R. J., Hashitani, H. Pacemaker mechanisms driving pyeloureteric peristalsis: modulatory role of interstitial cells. Advances in Experimental Medicine and Biology. 1124, 77-101 (2019).","journal-title":"Advances in Experimental Medicine and Biology"},{"issue":"5-6","key":"ref19","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1007\/s00424-016-1930-6","type":"journal-article","article-title":"Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators","volume":"469","author":"Hashitani","year":"2017","unstructured":"Hashitani, H. et al. Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators. Pflugers Archiv: European Journal of Physiology. 469 (5-6), 797-813 (2017).","journal-title":"Pflugers Archiv: European Journal of Physiology"},{"issue":"8","key":"ref20","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1038\/sj.bjp.0707535","type":"journal-article","article-title":"Role of Ca2+ entry and Ca2+ stores in atypical smooth muscle cell autorhythmicity in the mouse renal pelvis","volume":"152","author":"Lang","year":"2007","unstructured":"Lang, R. J., Hashitani, H., Tonta, M. A., Suzuki, H., Parkington, H. C. Role of Ca2+ entry and Ca2+ stores in atypical smooth muscle cell autorhythmicity in the mouse renal pelvis. British Journal of Pharmacology. 152 (8), 1248-1259 (2007).","journal-title":"British Journal of Pharmacology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1113\/jphysiol.2007.137034","type":"journal-article","article-title":"Spontaneous electrical and Ca2+ signals in typical and atypical smooth muscle cells and interstitial cell of Cajal-like cells of mouse renal pelvis","volume":"583","author":"Lang","year":"2007","unstructured":"Lang, R. J., Hashitani, H., Tonta, M. A., Parkington, H. C. , Suzuki, H. Spontaneous electrical and Ca2+ signals in typical and atypical smooth muscle cells and interstitial cell of Cajal-like cells of mouse renal pelvis. The Journal of Physiology. 583, 1049-1068 (2007).","journal-title":"The Journal of Physiology"},{"issue":"8","key":"ref22","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1111\/j.1476-5381.2009.00514.x","type":"journal-article","article-title":"Distinct effects of CGRP on typical and atypical smooth muscle cells involved in generating spontaneous contractions in the mouse renal pelvis","volume":"158","author":"Hashitani","year":"2009","unstructured":"Hashitani, H., Lang, R. J., Mitsui, R., Mabuchi, Y., Suzuki, H. Distinct effects of CGRP on typical and atypical smooth muscle cells involved in generating spontaneous contractions in the mouse renal pelvis. British Journal of Pharmacology. 158 (8), 2030-2045 (2009).","journal-title":"British Journal of Pharmacology"},{"issue":"7","key":"ref23","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1111\/j.1476-5381.2011.01730.x","type":"journal-article","article-title":"Potassium and ANO1\/ TMEM16A chloride channel profiles distinguish atypical and typical smooth muscle cells from interstitial cells in the mouse renal pelvis","volume":"165","author":"Iqbal","year":"2012","unstructured":"Iqbal, J. et al. Potassium and ANO1\/ TMEM16A chloride channel profiles distinguish atypical and typical smooth muscle cells from interstitial cells in the mouse renal pelvis. British Journal of Pharmacology. 165 (7), 2389-2408 (2012).","journal-title":"British Journal of Pharmacology"},{"issue":"15","key":"ref24","doi-asserted-by":"crossref","first-page":"3283","DOI":"10.1113\/JP278888","type":"journal-article","article-title":"Identification and classification of interstitial cells in the mouse renal pelvis","volume":"598","author":"Grainger","year":"2020","unstructured":"Grainger, N. et al. Identification and classification of interstitial cells in the mouse renal pelvis. Journal of Physiology. 598 (15), 3283-3307 (2020).","journal-title":"Journal of Physiology"},{"issue":"6","key":"ref25","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1002\/nau.24431","type":"journal-article","article-title":"Functional heterogeneity of PDGFR\u03b1 (+) cells in spontaneously active urogenital tissues","volume":"39","author":"Hashitani","year":"2020","unstructured":"Hashitani, H., Mitsui, R., Lang, R. Functional heterogeneity of PDGFR\u03b1 (+) cells in spontaneously active urogenital tissues. Neurourology and Urodynamics. 39 (6), 1667-1678 (2020).","journal-title":"Neurourology and Urodynamics"},{"issue":"15","key":"ref26","doi-asserted-by":"crossref","first-page":"3061","DOI":"10.1113\/JP280080","type":"journal-article","article-title":"ATYPICAL or INTERSTITIAL, take your PIC","volume":"598","author":"Hashitani","year":"2020","unstructured":"Hashitani, H., Lang, R. J. ATYPICAL or INTERSTITIAL, take your PIC. Journal of Physiology. 598 (15), 3061-3062 (2020).","journal-title":"Journal of Physiology"},{"issue":"8","key":"ref27","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1038\/sj.bjp.0707535","type":"journal-article","article-title":"Role of Ca2+ entry and Ca2+ stores in atypical smooth muscle cell autorhythmicity in the mouse renal pelvis","volume":"152","author":"Lang","year":"2007","unstructured":"Lang, R. J., Hashitani, H., Tonta, M. A., Suzuki, H., Parkington, H. C. Role of Ca2+ entry and Ca2+ stores in atypical smooth muscle cell autorhythmicity in the mouse renal pelvis. British Journal of Pharmacology. 152 (8), 1248-1259 (2007).","journal-title":"British Journal of Pharmacology"},{"issue":"7","key":"ref28","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1113\/EP085299","type":"journal-article","article-title":"Principles and standards for reporting animal experiments in The Journal of Physiology and Experimental Physiology","volume":"100","author":"Grundy","year":"2015","unstructured":"Grundy, D. Principles and standards for reporting animal experiments in The Journal of Physiology and Experimental Physiology. Experimental Physiology. 100 (7), 755-758 (2015).","journal-title":"Experimental Physiology"},{"issue":"143","key":"ref29","first-page":"1","type":"journal-article","article-title":"Applications of spatio-temporal mapping and particle analysis techniques to quantify intracellular Ca 2+ signaling in situ","volume":"2019","author":"Drumm","year":"2019","unstructured":"Drumm, B. T., Hennig, G. W., Baker, S. A., Sanders, K. M. Applications of spatio-temporal mapping and particle analysis techniques to quantify intracellular Ca 2+ signaling in situ. Journal of Visualized Experiments. 2019 (143), 1-13 (2019).","journal-title":"Journal of Visualized Experiments"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"102260","DOI":"10.1016\/j.ceca.2020.102260","type":"journal-article","article-title":"A high throughput machine-learning driven analysis of Ca2+ spatio-temporal maps","volume":"91","author":"Leigh","year":"2020","unstructured":"Leigh, W. A. et al. A high throughput machine-learning driven analysis of Ca2+ spatio-temporal maps. Cell Calcium. 91, 102260 (2020).","journal-title":"Cell Calcium"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/62040\/isolating-imaging-live-intact-pacemaker-regions-mouse-renal-pelvis","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:40:05Z","timestamp":1792618805000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/62040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,30]]},"references-count":30,"journal-issue":{"issue":"170","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/62040","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,30]]}}}