{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:40:03Z","timestamp":1792622403064,"version":"4.5.0"},"reference-count":43,"publisher":"MyJove Corporation","issue":"168","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/62093","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T19:01:48Z","timestamp":1613070108000},"source":"Crossref","is-referenced-by-count":7,"title":["Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model"],"prefix":"10.3791","author":[{"given":"Kevin","family":"Boerner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anny-Claude","family":"Luissint","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Parkos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,2,11]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1084\/jem.20050407","type":"journal-article","article-title":"The primary defect in experimental ileitis originates from a nonhematopoietic source","volume":"203","author":"Olson","year":"2006","unstructured":"Olson, T. S. et al. The primary defect in experimental ileitis originates from a nonhematopoietic source. Journal of Experimental Medicine. 203 (3), 541-552 (2006).","journal-title":"Journal of Experimental Medicine"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1097\/00054725-200407000-00023","type":"journal-article","article-title":"Mechanisms of natural tolerance in the intestine: implications for inflammatory bowel disease","volume":"10","author":"Jump","year":"2004","unstructured":"Jump, R. L., Levine, A. D. Mechanisms of natural tolerance in the intestine: implications for inflammatory bowel disease. Inflammatory Bowel Diseases. 10 (4), 462-478 (2004).","journal-title":"Inflammatory Bowel Diseases"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/S0016-5085(97)70174-4","type":"journal-article","article-title":"Clustering of increased small intestinal permeability in families with Crohn's disease","volume":"113","author":"Peeters","year":"1997","unstructured":"Peeters, M. et al. Clustering of increased small intestinal permeability in families with Crohn's disease. Gastroenterology. 113 (3), 802-807 (1997).","journal-title":"Gastroenterology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"628157","DOI":"10.1155\/2015\/628157","type":"journal-article","article-title":"Intestinal permeability in inflammatory bowel disease: Pathogenesis, clinical evaluation, and therapy of leaky gut","volume":"2015","author":"Michielan","year":"2015","unstructured":"Michielan, A., D'Inca, R. Intestinal permeability in inflammatory bowel disease: Pathogenesis, clinical evaluation, and therapy of leaky gut. Mediators of Inflammation. 2015 628157 (2015).","journal-title":"Mediators of Inflammation"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1196\/annals.1326.018","type":"journal-article","article-title":"Neutrophil transepithelial migration and epithelial barrier function in IBD: potential targets for inhibiting neutrophil trafficking","volume":"1072","author":"Chin","year":"2006","unstructured":"Chin, A. C., Parkos, C. A. Neutrophil transepithelial migration and epithelial barrier function in IBD: potential targets for inhibiting neutrophil trafficking. Annals of the New York Academy of Sciences. 1072, 276-287 (2006).","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"9853","key":"ref6","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1016\/S0140-6736(12)60026-9","type":"journal-article","article-title":"Crohn's disease","volume":"380","author":"Baumgart","year":"2012","unstructured":"Baumgart, D. C., Sandborn, W. J. Crohn's disease. Lancet. 380 (9853), 1590-1605 (2012).","journal-title":"Lancet"},{"issue":"9853","key":"ref7","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1016\/S0140-6736(12)60150-0","type":"journal-article","article-title":"Ulcerative colitis","volume":"380","author":"Ord\u00e1s","year":"2012","unstructured":"Ord\u00e1s, I., Eckmann, L., Talamini, M., Baumgart, D. C., Sandborn, W. J. Ulcerative colitis. Lancet. 380 (9853), 1606-1619 (2012).","journal-title":"Lancet"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1080\/00365521.2016.1235224","type":"journal-article","article-title":"Neutrophils in ulcerative colitis: A review of selected biomarkers and their potential therapeutic implications","volume":"52","author":"Muthas","year":"2017","unstructured":"Muthas, D. et al. Neutrophils in ulcerative colitis: A review of selected biomarkers and their potential therapeutic implications. Scandanavian Journal of Gastroenterology. 52 (2), 125-135 (2017).","journal-title":"Scandanavian Journal of Gastroenterology"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.gie.2018.08.018","type":"journal-article","article-title":"The emerging role of histologic disease activity assessment in ulcerative colitis","volume":"88","author":"Pai","year":"2018","unstructured":"Pai, R. K. et al. The emerging role of histologic disease activity assessment in ulcerative colitis. Gastrointestinal Endoscopy. 88 (6), 887-898 (2018).","journal-title":"Gastrointestinal Endoscopy"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1172\/JCI115473","type":"journal-article","article-title":"Neutrophil migration across a cultured intestinal epithelium. Dependence on a CD11b\/CD18-mediated event and enhanced efficiency in physiological direction","volume":"88","author":"Parkos","year":"1991","unstructured":"Parkos, C. A., Delp, C., Arnaout, M. A., Madara, J. L. Neutrophil migration across a cultured intestinal epithelium. Dependence on a CD11b\/CD18-mediated event and enhanced efficiency in physiological direction. The Journal of Clinical Investigation. 88 (5), 1605-1612 (1991).","journal-title":"The Journal of Clinical Investigation"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1111\/imr.12446","type":"journal-article","article-title":"Pathobiology of neutrophil-epithelial interactions","volume":"273","author":"Brazil","year":"2016","unstructured":"Brazil, J. C., Parkos, C. A. Pathobiology of neutrophil-epithelial interactions. Immunological Reviews. 273 (1), 94-111 (2016).","journal-title":"Immunological Reviews"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s12876-019-1002-4","type":"journal-article","article-title":"The Ussing chamber system for measuring intestinal permeability in health and disease","volume":"19","author":"Thomson","year":"2019","unstructured":"Thomson, A. et al. The Ussing chamber system for measuring intestinal permeability in health and disease. BMC Gastroenterology. 19 (1), 98 (2019).","journal-title":"BMC Gastroenterology"},{"issue":"140","key":"ref13","first-page":"e57032","type":"journal-article","article-title":"In vitro and in vivo approaches to determine intestinal epithelial cell permeability","author":"Li","year":"2018","unstructured":"Li, B. R. et al. In vitro and in vivo approaches to determine intestinal epithelial cell permeability. Journal of Visualized Experiments. (140), e57032 (2018).","journal-title":"Journal of Visualized Experiments"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1177\/2211068214561025","type":"journal-article","article-title":"TEER measurement techniques for in vitro barrier model systems","volume":"20","author":"Srinivasan","year":"2015","unstructured":"Srinivasan, B. et al. TEER measurement techniques for in vitro barrier model systems. Journal of Laboratory Automation. 20 (2), 107-126 (2015).","journal-title":"Journal of Laboratory Automation"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1091\/mbc.E18-08-0531","type":"journal-article","article-title":"Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation","volume":"30","author":"Fan","year":"2019","unstructured":"Fan, S. et al. Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation. Molecular Biology of the Cell. 30 (5), 566-578 (2019).","journal-title":"Molecular Biology of the Cell"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1016\/j.ajpath.2016.02.001","type":"journal-article","article-title":"Neutrophil-epithelial interactions: A double-edged sword","volume":"186","author":"Parkos","year":"2016","unstructured":"Parkos, C. A. Neutrophil-epithelial interactions: A double-edged sword. American Journal of Pathology. 186 (6), 1404-1416 (2016).","journal-title":"American Journal of Pathology"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1007\/s10620-015-3935-y","type":"journal-article","article-title":"Assessment of the intestinal barrier with five different permeability tests in healthy C57BL\/6J and BALB\/cJ mice","volume":"61","author":"Volynets","year":"2016","unstructured":"Volynets, V. et al. Assessment of the intestinal barrier with five different permeability tests in healthy C57BL\/6J and BALB\/cJ mice. Digital Diseases and Sciences. 61 (3), 737-746 (2016).","journal-title":"Digital Diseases and Sciences"},{"issue":"139","key":"ref18","first-page":"e57037","type":"journal-article","article-title":"An optimized evans blue protocol to assess vascular leak in the mouse","author":"Wick","year":"2018","unstructured":"Wick, M. J., Harral, J. W., Loomis, Z. L., & Dempsey, E. C. An optimized evans blue protocol to assess vascular leak in the mouse. Journal of Visualized Experiments. (139), e57037 (2018).","journal-title":"Journal of Visualized Experiments"},{"issue":"3","key":"ref19","first-page":"271","type":"journal-article","article-title":"Role of cytokines in experimental colitis: relation to intestinal permeability","volume":"58","author":"Tateishi","year":"1997","unstructured":"Tateishi, H., Mitsuyama, K., Toyonaga, A., Tomoyose, M., Tanikawa, K. Role of cytokines in experimental colitis: relation to intestinal permeability. Digestion. 58 (3), 271-281 (1997).","journal-title":"Digestion"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/aps.2010.225","type":"journal-article","article-title":"A protective effect of melatonin on intestinal permeability is induced by diclofenac via regulation of mitochondrial function in mice","volume":"32","author":"Mei","year":"2011","unstructured":"Mei, Q., Diao, L., Xu, J. M., Liu, X. C., Jin, J. A protective effect of melatonin on intestinal permeability is induced by diclofenac via regulation of mitochondrial function in mice. Acta Pharmacologica Sinica. 32 (4), 495-502 (2011).","journal-title":"Acta Pharmacologica Sinica"},{"issue":"119","key":"ref21","first-page":"e55095","type":"journal-article","article-title":"Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis","author":"Vargas Robles","year":"2017","unstructured":"Vargas Robles, H., Castro Ochoa, K. F., Nava, P., Silva Olivares, A., Shibayama, M., Schnoor, M. Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis. J. Vis. Exp. (119), e55095 (2017).","journal-title":"Journal of Visualized Experiments"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1053\/j.gastro.2009.04.010","type":"journal-article","article-title":"Salmonella induces flagellin- and MyD88-dependent migration of bacteria-capturing dendritic cells into the gut lumen","volume":"137","author":"Arques","year":"2009","unstructured":"Arques, J. L. et al. Salmonella induces flagellin- and MyD88-dependent migration of bacteria-capturing dendritic cells into the gut lumen. Gastroenterology. 137 (2), 579-587 (2009).","journal-title":"Gastroenterology"},{"issue":"11","key":"ref23","doi-asserted-by":"crossref","first-page":"7161","DOI":"10.1128\/IAI.73.11.7161-7169.2005","type":"journal-article","article-title":"Analysis of the contribution of Salmonella pathogenicity islands 1 and 2 to enteric disease progression using a novel bovine ileal loop model and a murine model of infectious enterocolitis","volume":"73","author":"Coombes","year":"2005","unstructured":"Coombes, B. K. et al. Analysis of the contribution of Salmonella pathogenicity islands 1 and 2 to enteric disease progression using a novel bovine ileal loop model and a murine model of infectious enterocolitis. Infection and Immunity. 73 (11), 7161-7169 (2005).","journal-title":"Infection and Immunity"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"2815","DOI":"10.1128\/IAI.67.6.2815-2821.1999","type":"journal-article","article-title":"Evaluation of Salmonella typhimurium mutants in a model of experimental gastroenteritis","volume":"67","author":"Everest","year":"1999","unstructured":"Everest, P. et al. Evaluation of Salmonella typhimurium mutants in a model of experimental gastroenteritis. Infection and Immunity. 67 (6), 2815-2821 (1999).","journal-title":"Infection and Immunity"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1128\/IAI.66.2.747-755.1998","type":"journal-article","article-title":"Comprehensive study of the intestinal stage of listeriosis in a rat ligated ileal loop system","volume":"66","author":"Pron","year":"1998","unstructured":"Pron, B. et al. Comprehensive study of the intestinal stage of listeriosis in a rat ligated ileal loop system. Infection and Immunity. 66 (2), 747-755 (1998).","journal-title":"Infection and Immunity"},{"issue":"10","key":"ref26","doi-asserted-by":"crossref","first-page":"2702","DOI":"10.1172\/JCI24970","type":"journal-article","article-title":"Epithelial myosin light chain kinase-dependent barrier dysfunction mediates T cell activation-induced diarrhea in vivo","volume":"115","author":"Clayburgh","year":"2005","unstructured":"Clayburgh, D. R. et al. Epithelial myosin light chain kinase-dependent barrier dysfunction mediates T cell activation-induced diarrhea in vivo. The Journal of Clinical Investigation. 115 (10), 2702-2715 (2005).","journal-title":"The Journal of Clinical Investigation"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1182\/blood.V58.3.658.658","type":"journal-article","article-title":"Leukotriene B4 is a potent and stereospecific stimulator of neutrophil chemotaxis and adherence","volume":"58","author":"Palmblad","year":"1981","unstructured":"Palmblad, J. et al. Leukotriene B4 is a potent and stereospecific stimulator of neutrophil chemotaxis and adherence. Blood. 58 (3), 658-661 (1981).","journal-title":"Blood"},{"issue":"12","key":"ref28","doi-asserted-by":"crossref","first-page":"11665","DOI":"10.1074\/jbc.M412650200","type":"journal-article","article-title":"Junctional adhesion molecule 1 regulates epithelial cell morphology through effects on beta1 integrins and Rap1 activity","volume":"280","author":"Mandell","year":"2005","unstructured":"Mandell, K. J., Babbin, B. A., Nusrat, A., Parkos, C. A. Junctional adhesion molecule 1 regulates epithelial cell morphology through effects on beta1 integrins and Rap1 activity. The Journal of Biological Chemistry. 280 (12), 11665-11674 (2005).","journal-title":"The Journal of Biological Chemistry"},{"issue":"13","key":"ref29","doi-asserted-by":"crossref","first-page":"3067","DOI":"10.1084\/jem.20071416","type":"journal-article","article-title":"JAM-A regulates permeability and inflammation in the intestine in vivo","volume":"204","author":"Laukoetter","year":"2007","unstructured":"Laukoetter, M. G. et al. JAM-A regulates permeability and inflammation in the intestine in vivo. Journal of Experimental Medicine. 204 (13), 3067-3076 (2007).","journal-title":"Journal of Experimental Medicine"},{"issue":"20","key":"ref30","type":"journal-article","article-title":"Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model","volume":"3","author":"Flemming","year":"2018","unstructured":"Flemming, S., Luissint, A. C., Nusrat, A., Parkos, C. A. Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model. Journal of Clinical Investigation Insight. 3 (20), (2018).","journal-title":"Journal of Clinical Investigation Insight"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s00281-014-0421-0","type":"journal-article","article-title":"JAM-related proteins in mucosal homeostasis and inflammation","volume":"36","author":"Luissint","year":"2014","unstructured":"Luissint, A. C., Nusrat, A., Parkos, C. A. JAM-related proteins in mucosal homeostasis and inflammation. Seminars in Immunopathology. 36 (2), 211-226 (2014).","journal-title":"Seminars in Immunopathology"},{"issue":"4","key":"ref32","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1258\/la.2010.009085","type":"journal-article","article-title":"Isoflurane and sevoflurane provide equally effective anaesthesia in laboratory mice","volume":"44","author":"Cesarovic","year":"2010","unstructured":"Cesarovic, N. et al. Isoflurane and sevoflurane provide equally effective anaesthesia in laboratory mice. Lab Animal. 44 (4), 329-336 (2010).","journal-title":"Lab Animal"},{"key":"ref33","first-page":"e5024","type":"journal-article","article-title":"Introduction to the Microplate Reader","year":"2020","unstructured":"JoVE Science Education Database. General Laboratory Techniques. Introduction to the Microplate Reader. JoVE, Cambridge, MA, (2020).","journal-title":"Journal of Visualized Experiments"},{"issue":"12","key":"ref34","type":"journal-article","article-title":"Targeting epithelium-expressed sialyl Lewis glycans improves colonic mucosal wound healing and protects against colitis","volume":"5","author":"Kelm","year":"2020","unstructured":"Kelm, M. et al. Targeting epithelium-expressed sialyl Lewis glycans improves colonic mucosal wound healing and protects against colitis. Journal of Clinical Investigation Insight. 5 (12) (2020).","journal-title":"Journal of Clinical Investigation Insight"},{"key":"ref35","type":"journal-article","article-title":"Neutrophil expressed CD47 regulates CD11b\/CD18-dependent neutrophil transepithelial migration in the intestine in vivo","author":"Azcutia","year":"2020","unstructured":"Azcutia, V. et al. Neutrophil expressed CD47 regulates CD11b\/CD18-dependent neutrophil transepithelial migration in the intestine in vivo. Mucosal Immunology. 10.1038\/s41385-020-0316-4 (2020).","journal-title":"Mucosal Immunology"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"0150606","DOI":"10.1371\/journal.pone.0150606","type":"journal-article","article-title":"A protocol for the comprehensive flow cytometric analysis of immune cells in normal and inflamed murine non-lymphoid tissues","volume":"11","author":"Yu","year":"2016","unstructured":"Yu, Y. R. et al. A protocol for the comprehensive flow cytometric analysis of immune cells in normal and inflamed murine non-lymphoid tissues. PloS One. 11 (3), e0150606 (2016).","journal-title":"PloS One"},{"issue":"10","key":"ref37","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1161\/ATVBAHA.107.147694","type":"journal-article","article-title":"JAM family and related proteins in leukocyte migration (Vestweber series)","volume":"27","author":"Bradfield","year":"2007","unstructured":"Bradfield, P. F., Nourshargh, S., Aurrand-Lions, M., Imhof, B. A. JAM family and related proteins in leukocyte migration (Vestweber series). Arteriosclerosis, Thrombosis, and Vascular Biology. 27 (10), 2104-2112 (2007).","journal-title":"Arteriosclerosis, Thrombosis, and Vascular Biology"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1152\/physrev.00004.2017","type":"journal-article","article-title":"Junctional Adhesion Molecules (JAMs): Cell adhesion receptors with pleiotropic functions in cell physiology and development","volume":"97","author":"Ebnet","year":"2017","unstructured":"Ebnet, K. Junctional Adhesion Molecules (JAMs): Cell adhesion receptors with pleiotropic functions in cell physiology and development. Physiological Reviews. 97 (4), 1529-1554 (2017).","journal-title":"Physiological Reviews"},{"issue":"6","key":"ref39","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1016\/j.jhep.2019.06.017","type":"journal-article","article-title":"FXR modulates the gut-vascular barrier by regulating the entry sites for bacterial translocation in experimental cirrhosis","volume":"71","author":"Sorribas","year":"2019","unstructured":"Sorribas, M. et al. FXR modulates the gut-vascular barrier by regulating the entry sites for bacterial translocation in experimental cirrhosis. Journal of Hepatology. 71 (6), 1126-1140 (2019).","journal-title":"Journal of Hepatology"},{"issue":"15","key":"ref40","doi-asserted-by":"crossref","first-page":"3709","DOI":"10.21769\/BioProtoc.3709","type":"journal-article","article-title":"In vivo Blood-brain Barrier Permeability Assays Using Clostridium perfringens Epsilon Toxin","volume":"10","author":"Mazzucco","year":"2020","unstructured":"Mazzucco, M. R., Vartanian, T., Linden, J. R. In vivo Blood-brain Barrier Permeability Assays Using Clostridium perfringens Epsilon Toxin. Bio-Protocol. 10 (15), e3709 (2020).","journal-title":"Bio-Protocol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3389\/fncel.2015.00392","type":"journal-article","article-title":"Breaking down the barriers: the gut microbiome, intestinal permeability and stress-related psychiatric disorders","volume":"9","author":"Kelly","year":"2015","unstructured":"Kelly, J. R. et al. Breaking down the barriers: the gut microbiome, intestinal permeability and stress-related psychiatric disorders. Frontiers in Cellular Neuroscience. 9, 392 (2015).","journal-title":"Frontiers in Cellular Neuroscience"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s13229-016-0110-z","type":"journal-article","article-title":"Blood-brain barrier and intestinal epithelial barrier alterations in autism spectrum disorders","volume":"7","author":"Fiorentino","year":"2016","unstructured":"Fiorentino, M. et al. Blood-brain barrier and intestinal epithelial barrier alterations in autism spectrum disorders. Molecular Autism. 7 (1), 49 (2016).","journal-title":"Molecular Autism"},{"issue":"2","key":"ref43","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1096\/fj.201902542R","type":"journal-article","article-title":"Regulation of neutrophil function by selective targeting of glycan epitopes expressed on the integrin CD11b\/CD18","volume":"34","author":"Kelm","year":"2020","unstructured":"Kelm, M. et al. Regulation of neutrophil function by selective targeting of glycan epitopes expressed on the integrin CD11b\/CD18. FASEB Journal : An Official Publication of the Federation of American Societies for Experimental Biology. 34 (2), 2326-2343 (2020).","journal-title":"FASEB Journal : An Official Publication of the Federation of American Societies for Experimental Biology"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/62093\/functional-assessment-intestinal-permeability-neutrophil","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:04:46Z","timestamp":1792620286000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/62093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,11]]},"references-count":43,"journal-issue":{"issue":"168","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/62093","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,11]]}}}