{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:39:02Z","timestamp":1792622342023,"version":"4.5.0"},"reference-count":45,"publisher":"MyJove Corporation","issue":"168","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/62151","type":"journal-article","created":{"date-parts":[[2021,2,13]],"date-time":"2021-02-13T19:00:18Z","timestamp":1613242818000},"source":"Crossref","is-referenced-by-count":3,"title":["Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes"],"prefix":"10.3791","author":[{"given":"Jeremy P.","family":"Goering","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dona Greta","family":"Isai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andras","family":"Czirok","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irfan","family":"Saadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,2,13]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1242\/dev.067082","type":"journal-article","article-title":"Palatogenesis: morphogenetic and molecular mechanisms of secondary palate development","volume":"139","author":"Bush","year":"2012","unstructured":"Bush, J. O., Jiang, R. Palatogenesis: morphogenetic and molecular mechanisms of secondary palate development. Development. 139 (2), 231-243 (2012).","journal-title":"Development"},{"issue":"9703","key":"ref2","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1016\/S0140-6736(09)60695-4","type":"journal-article","article-title":"Cleft lip and palate","volume":"374","author":"Mossey","year":"2009","unstructured":"Mossey, P. A., Little, J., Munger, R. G., Dixon, M. J., Shaw, W. C. Cleft lip and palate. Lancet. 374 (9703), 1773-1785 (2009).","journal-title":"Lancet"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/bs.ctdb.2015.07.002","type":"journal-article","article-title":"Cellular and molecular mechanisms of palatogenesis","volume":"115","author":"Lan","year":"2015","unstructured":"Lan, Y., Xu, J., Jiang, R. Cellular and molecular mechanisms of palatogenesis. Current Topics in Developmental Biology. 115, 59-84 (2015).","journal-title":"Current Topics in Developmental Biology"},{"issue":"11","key":"ref4","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1177\/0022034517703580","type":"journal-article","article-title":"Molecular and cellular mechanisms of palate development","volume":"96","author":"Li","year":"2017","unstructured":"Li, C., Lan, Y., Jiang, R. Molecular and cellular mechanisms of palate development. Journal of Dental Research. 96 (11), 1184-1191 (2017).","journal-title":"Journal of Dental Research"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0070-2153(08)00602-9","type":"journal-article","article-title":"The etiopathogenesis of cleft lip and cleft palate: usefulness and caveats of mouse models","volume":"84","author":"Gritli-Linde","year":"2008","unstructured":"Gritli-Linde, A. The etiopathogenesis of cleft lip and cleft palate: usefulness and caveats of mouse models. Current Topics in Developmental Biology. 84, 37-138 (2008).","journal-title":"Current Topics in Developmental Biology"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1177\/0022034508327868","type":"journal-article","article-title":"Biological mechanisms in palatogenesis and cleft palate","volume":"88","author":"Meng","year":"2009","unstructured":"Meng, L., Bian, Z., Torensma, R., Von den Hoff, J. W. Biological mechanisms in palatogenesis and cleft palate. Journal of Dental Research. 88 (1), 22-33 (2009).","journal-title":"Journal of Dental Research"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/nrg2933","type":"journal-article","article-title":"Cleft lip and palate: understanding genetic and environmental influences","volume":"12","author":"Dixon","year":"2011","unstructured":"Dixon, M. J., Marazita, M. L., Beaty, T. H., Murray, J. C. Cleft lip and palate: understanding genetic and environmental influences. Nature Reviews Genetics. 12 (3), 167-178 (2011).","journal-title":"Nature Reviews Genetics"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1002\/dvdy.24341","type":"journal-article","article-title":"Toward an orofacial gene regulatory network","volume":"245","author":"Kousa","year":"2016","unstructured":"Kousa, Y. A., Schutte, B. C. Toward an orofacial gene regulatory network. Developmental Dynamics. 245 (3), 220-232 (2016).","journal-title":"Developmental Dynamics"},{"issue":"7","key":"ref9","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1002\/dvdy.22339","type":"journal-article","article-title":"Mesenchymal cell remodeling during mouse secondary palate reorientation","volume":"239","author":"Jin","year":"2010","unstructured":"Jin, J. Z. et al. Mesenchymal cell remodeling during mouse secondary palate reorientation. Developmental Dynamics. 239 (7), 2110-2117 (2010).","journal-title":"Developmental Dynamics"},{"issue":"7","key":"ref10","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1002\/dvdy.22670","type":"journal-article","article-title":"Histomorphological study of palatal shelf elevation during murine secondary palate formation","volume":"240","author":"Yu","year":"2011","unstructured":"Yu, K., Ornitz, D. M. Histomorphological study of palatal shelf elevation during murine secondary palate formation. Developmental Dynamics. 240 (7), 1737-1744 (2011).","journal-title":"Developmental Dynamics"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3389\/fphys.2016.00392","type":"journal-article","article-title":"Mesenchymal remodeling during palatal shelf elevation revealed by extracellular matrix and F-actin expression patterns","volume":"7","author":"Chiquet","year":"2016","unstructured":"Chiquet, M., Blumer, S., Angelini, M., Mitsiadis, T. A., Katsaros, C. Mesenchymal remodeling during palatal shelf elevation revealed by extracellular matrix and F-actin expression patterns. Frontiers in Physiology. 7, 392 (2016).","journal-title":"Frontiers in Physiology"},{"issue":"11","key":"ref12","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1177\/0022034517726079","type":"journal-article","article-title":"ARHGAP29 mutation is associated with abnormal oral epithelial adhesions","volume":"96","author":"Paul","year":"2017","unstructured":"Paul, B. J. et al. ARHGAP29 mutation is associated with abnormal oral epithelial adhesions. Journal of Dental Research. 96 (11), 1298-1305 (2017).","journal-title":"Journal of Dental Research"},{"issue":"5","key":"ref13","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1093\/hmg\/ddaa002","type":"journal-article","article-title":"SPECC1L regulates palate development downstream of IRF6","volume":"29","author":"Hall","year":"2020","unstructured":"Hall, E. G. et al. SPECC1L regulates palate development downstream of IRF6. Human Molecular Genetics. 29 (5), 845-858 (2020).","journal-title":"Human Molecular Genetics"},{"issue":"2","key":"ref14","first-page":"176","type":"journal-article","article-title":"Closure of the secondary palate in three strains of mice","volume":"4","author":"Walker","year":"1956","unstructured":"Walker, B. E., Fraser, F. C. Closure of the secondary palate in three strains of mice. Journal of Embryology and Experimental Morphology. 4 (2), 176-189 (1956).","journal-title":"Journal of Embryology and Experimental Morphology"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s00441-008-0612-x","type":"journal-article","article-title":"Analysis of Zfhx1a mutant mice reveals palatal shelf contact-independent medial edge epithelial differentiation during palate fusion","volume":"333","author":"Jin","year":"2008","unstructured":"Jin, J. Z., Li, Q., Higashi, Y., Darling, D. S., Ding, J. Analysis of Zfhx1a mutant mice reveals palatal shelf contact-independent medial edge epithelial differentiation during palate fusion. Cell Tissue Research. 333 (1), 29-38 (2008).","journal-title":"Cell Tissue Research"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"59463","DOI":"10.1371\/journal.pone.0059463","type":"journal-article","article-title":"The etiology of cleft palate formation in BMP7-deficient mice","volume":"8","author":"Kouskoura","year":"2013","unstructured":"Kouskoura, T. et al. The etiology of cleft palate formation in BMP7-deficient mice. PLoS One. 8 (3), e59463 (2013).","journal-title":"PLoS One"},{"issue":"13","key":"ref17","first-page":"2344","type":"journal-article","article-title":"Golgb1 regulates protein glycosylation and is crucial for mammalian palate development","volume":"143","author":"Lan","year":"2016","unstructured":"Lan, Y., Zhang, N., Liu, H., Xu, J., Jiang, R. Golgb1 regulates protein glycosylation and is crucial for mammalian palate development. Development. 143 (13), 2344-2355 (2016).","journal-title":"Development"},{"issue":"23","key":"ref18","doi-asserted-by":"crossref","first-page":"3871","DOI":"10.1242\/dev.025767","type":"journal-article","article-title":"Wnt5a regulates directional cell migration and cell proliferation via Ror2-mediated noncanonical pathway in mammalian palate development","volume":"135","author":"He","year":"2008","unstructured":"He, F. et al. Wnt5a regulates directional cell migration and cell proliferation via Ror2-mediated noncanonical pathway in mammalian palate development. Development. 135 (23), 3871-3879 (2008).","journal-title":"Development"},{"issue":"12","key":"ref19","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1177\/0022034519872478","type":"journal-article","article-title":"Requirement of hyaluronan synthase-2 in craniofacial and palate development","volume":"98","author":"Lan","year":"2019","unstructured":"Lan, Y., Qin, C., Jiang, R. Requirement of hyaluronan synthase-2 in craniofacial and palate development. Journal of Dental Research. 98 (12), 1367-1375 (2019).","journal-title":"Journal of Dental Research"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ydbio.2019.09.004","type":"journal-article","article-title":"Hyaluronic acid is required for palatal shelf movement and its interaction with the tongue during palatal shelf elevation","volume":"457","author":"Yonemitsu","year":"2020","unstructured":"Yonemitsu, M. A., Lin, T. Y., Yu, K. Hyaluronic acid is required for palatal shelf movement and its interaction with the tongue during palatal shelf elevation. Developmental Biology. 457 (1), 57-68 (2020).","journal-title":"Developmental Biology"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1038\/s41598-021-81123-9","type":"journal-article","article-title":"SPECC1L-deficient palate mesenchyme cells show speed and directionality defect","volume":"11","author":"Goering","year":"2021","unstructured":"Goering, J. P. et al. SPECC1L-deficient palate mesenchyme cells show speed and directionality defect. Scientific Reports. 11 (1), 1452 (2021).","journal-title":"Scientific Reports"},{"issue":"9","key":"ref22","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1101\/gad.238709.114","type":"journal-article","article-title":"PI3K-mediated PDGFRalpha signaling regulates survival and proliferation in skeletal development through p53-dependent intracellular pathways","volume":"28","author":"Fantauzzo","year":"2014","unstructured":"Fantauzzo, K. A., Soriano, P. PI3K-mediated PDGFRalpha signaling regulates survival and proliferation in skeletal development through p53-dependent intracellular pathways. Genes and Development. 28 (9), 1005-1017 (2014).","journal-title":"Genes and Development"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.devcel.2014.10.005","type":"journal-article","article-title":"SRF regulates craniofacial development through selective recruitment of MRTF cofactors by PDGF signaling","volume":"31","author":"Vasudevan","year":"2014","unstructured":"Vasudevan, H. N., Soriano, P. SRF regulates craniofacial development through selective recruitment of MRTF cofactors by PDGF signaling. Developmental Cell. 31 (3), 332-344 (2014).","journal-title":"Developmental Cell"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"07186","DOI":"10.7554\/eLife.07186","type":"journal-article","article-title":"Receptor tyrosine kinases modulate distinct transcriptional programs by differential usage of intracellular pathways","volume":"4","author":"Vasudevan","year":"2015","unstructured":"Vasudevan, H. N., Mazot, P., He, F., Soriano, P. Receptor tyrosine kinases modulate distinct transcriptional programs by differential usage of intracellular pathways. Elife. 4, e07186 (2015).","journal-title":"Elife"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"1559325818786822","DOI":"10.1177\/1559325818786822","type":"journal-article","article-title":"2,3,7,8-Tetrachlorodibenzo-p-dioxin and TGFbeta3-mediated mouse embryonic palatal mesenchymal cells","volume":"17","author":"Gao","year":"2019","unstructured":"Gao, L. et al. 2,3,7,8-Tetrachlorodibenzo-p-dioxin and TGFbeta3-mediated mouse embryonic palatal mesenchymal cells. Dose Response. 17 (1), 1559325818786822 (2019).","journal-title":"Dose Response"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"929","DOI":"10.3389\/fphys.2017.00929","type":"journal-article","article-title":"Hoxa2 inhibits bone morphogenetic protein signaling during osteogenic differentiation of the palatal mesenchyme","volume":"8","author":"Iyyanar","year":"2017","unstructured":"Iyyanar, P. P. R., Nazarali, A. J. Hoxa2 inhibits bone morphogenetic protein signaling during osteogenic differentiation of the palatal mesenchyme. Frontiers in Physiology. 8, 929 (2017).","journal-title":"Frontiers in Physiology"},{"issue":"6","key":"ref27","first-page":"5339","type":"journal-article","article-title":"Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the beta-catenin pathway","volume":"14","author":"Jiang","year":"2017","unstructured":"Jiang, Z., Pan, L., Chen, X., Chen, Z., Xu, D. Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the beta-catenin pathway. Experimental and Therapeutic Medicine. 14 (6), 5339-5344 (2017).","journal-title":"Experimental and Therapeutic Medicine"},{"issue":"6","key":"ref28","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1002\/bdrb.21130","type":"journal-article","article-title":"Negative interplay of retinoic acid and TGF-beta signaling mediated by TG-interacting factor to modulate mouse embryonic palate mesenchymal-cell proliferation","volume":"101","author":"Liu","year":"2014","unstructured":"Liu, X. et al. Negative interplay of retinoic acid and TGF-beta signaling mediated by TG-interacting factor to modulate mouse embryonic palate mesenchymal-cell proliferation. Birth Defects Research Part B: Developmental and Reproductive Toxicology. 101 (6), 403-409 (2014).","journal-title":"Birth Defects Research Part B: Developmental and Reproductive Toxicology"},{"issue":"18","key":"ref29","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1101\/gad.1963210","type":"journal-article","article-title":"Ephrin-B1 forward signaling regulates craniofacial morphogenesis by controlling cell proliferation across Eph-ephrin boundaries","volume":"24","author":"Bush","year":"2010","unstructured":"Bush, J. O., Soriano, P. Ephrin-B1 forward signaling regulates craniofacial morphogenesis by controlling cell proliferation across Eph-ephrin boundaries. Genes & Development. 24 (18), 2068-2080 (2010).","journal-title":"Genes & Development"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"588901","DOI":"10.3389\/fphys.2020.588901","type":"journal-article","article-title":"Pdgfra and Pdgfrb genetically interact in the murine neural crest cell lineage to regulate migration and proliferation","volume":"11","author":"Mo","year":"2020","unstructured":"Mo, J., Long, R., Fantauzzo, K. A. Pdgfra and Pdgfrb genetically interact in the murine neural crest cell lineage to regulate migration and proliferation. Frontiers in Physiology. 11, 588901 (2020).","journal-title":"Frontiers in Physiology"},{"issue":"9","key":"ref31","doi-asserted-by":"crossref","first-page":"1003851","DOI":"10.1371\/journal.pgen.1003851","type":"journal-article","article-title":"A critical role for PDGFRalpha signaling in medial nasal process development","volume":"9","author":"He","year":"2013","unstructured":"He, F., Soriano, P. A critical role for PDGFRalpha signaling in medial nasal process development. PLoS Genetics. 9 (9), e1003851 (2013).","journal-title":"PLoS Genetics"},{"issue":"6","key":"ref32","doi-asserted-by":"crossref","first-page":"0179078","DOI":"10.1371\/journal.pone.0179078","type":"journal-article","article-title":"Generation of an immortalized mouse embryonic palatal mesenchyme cell line","volume":"12","author":"Fantauzzo","year":"2017","unstructured":"Fantauzzo, K. A., Soriano, P. Generation of an immortalized mouse embryonic palatal mesenchyme cell line. PLoS One. 12 (6), e0179078 (2017).","journal-title":"PLoS One"},{"issue":"1","key":"ref33","first-page":"1","type":"journal-article","article-title":"Live cell image segmentation","volume":"42","author":"Wu","year":"1995","unstructured":"Wu, K., Gauthier, D., Levine, M. D. Live cell image segmentation. IEEE Transactions on Biomedical Engineering. 42 (1), 1-12 (1995).","journal-title":"IEEE Transactions on Biomedical Engineering"},{"issue":"11","key":"ref34","doi-asserted-by":"crossref","first-page":"1005818","DOI":"10.1371\/journal.pcbi.1005818","type":"journal-article","article-title":"The role of Allee effect in modelling post resection recurrence of glioblastoma","volume":"13","author":"Neufeld","year":"2017","unstructured":"Neufeld, Z. et al. The role of Allee effect in modelling post resection recurrence of glioblastoma. PLoS Computational Biology. 13 (11), e1005818 (2017).","journal-title":"PLoS Computational Biology"},{"issue":"6","key":"ref35","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1007\/s10439-005-3037-7","type":"journal-article","article-title":"A digital image-based method for computational tissue fate mapping during early avian morphogenesis","volume":"33","author":"Zamir","year":"2005","unstructured":"Zamir, E. A., Czirok, A., Rongish, B. J., Little, C. D. A digital image-based method for computational tissue fate mapping during early avian morphogenesis. Annals of Biomedical Engineering. 33 (6), 854-865 (2005).","journal-title":"Annals of Biomedical Engineering"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"10404","DOI":"10.1038\/s41598-017-10094-7","type":"journal-article","article-title":"Optical-flow based non-invasive analysis of cardiomyocyte contractility","volume":"7","author":"Czirok","year":"2017","unstructured":"Czirok, A. et al. Optical-flow based non-invasive analysis of cardiomyocyte contractility. Scientific Reports. 7 (1), 10404 (2017).","journal-title":"Scientific Reports"},{"key":"ref37","first-page":"2840","type":"journal-article","article-title":"Interferon regulatory factor 6 regulates keratinocyte migration","volume":"127","author":"Biggs","year":"2014","unstructured":"Biggs, L. C. et al. Interferon regulatory factor 6 regulates keratinocyte migration. Journal of Cell Science. 127 (Pt 13), 2840-2848 (2014).","journal-title":"Journal of Cell Science"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"75006","DOI":"10.1088\/1367-2630\/15\/7\/075006","type":"journal-article","article-title":"Collective cell streams in epithelial monolayers depend on cell adhesion","volume":"15","author":"Czirok","year":"2013","unstructured":"Czirok, A., Varga, K., Mehes, E., Szabo, A. Collective cell streams in epithelial monolayers depend on cell adhesion. New Journal of Physics. 15, 75006 (2013).","journal-title":"New Journal of Physics"},{"issue":"4","key":"ref39","doi-asserted-by":"crossref","first-page":"046007","DOI":"10.1088\/1478-3975\/7\/4\/046007","type":"journal-article","article-title":"Collective cell motion in endothelial monolayers","volume":"7","author":"Szabo","year":"2010","unstructured":"Szabo, A. et al. Collective cell motion in endothelial monolayers. Physical Biology. 7 (4), 046007 (2010).","journal-title":"Physical Biology"},{"issue":"9","key":"ref40","doi-asserted-by":"crossref","first-page":"0203203","DOI":"10.1371\/journal.pone.0203203","type":"journal-article","article-title":"Software tools for cell culture-related 3D printed structures","volume":"13","author":"Gulyas","year":"2018","unstructured":"Gulyas, M., Csiszer, M., Mehes, E., Czirok, A. Software tools for cell culture-related 3D printed structures. PLoS One. 13 (9), e0203203 (2018).","journal-title":"PLoS One"},{"issue":"3","key":"ref41","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.chembiol.2005.03.001","type":"journal-article","article-title":"Scratch n' screen for inhibitors of cell migration","volume":"12","author":"Soderholm","year":"2005","unstructured":"Soderholm, J., Heald, R. Scratch n' screen for inhibitors of cell migration. Chemistry & Biology. 12 (3), 263-265 (2005).","journal-title":"Chemistry & Biology"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1177\/2211068211426550","type":"journal-article","article-title":"Advances in wound-healing assays for probing collective cell migration","volume":"17","author":"Riahi","year":"2012","unstructured":"Riahi, R., Yang, Y., Zhang, D. D., Wong, P. K. Advances in wound-healing assays for probing collective cell migration. Journal of Laboratory Automation. 17 (1), 59-65 (2012).","journal-title":"Journal of Laboratory Automation"},{"issue":"3","key":"ref43","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/cyto.a.23249","type":"journal-article","article-title":"Untangling cell tracks: Quantifying cell migration by time lapse image data analysis","volume":"93","author":"Svensson","year":"2018","unstructured":"Svensson, C. M., Medyukhina, A., Belyaev, I., Al-Zaben, N., Figge, M. T. Untangling cell tracks: Quantifying cell migration by time lapse image data analysis. Cytometry Part A. 93 (3), 357-370 (2018).","journal-title":"Cytometry Part A"},{"issue":"21","key":"ref44","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1101\/gad.288746.116","type":"journal-article","article-title":"PDGFRbeta regulates craniofacial development through homodimers and functional heterodimers with PDGFRalpha","volume":"30","author":"Fantauzzo","year":"2016","unstructured":"Fantauzzo, K. A., Soriano, P. PDGFRbeta regulates craniofacial development through homodimers and functional heterodimers with PDGFRalpha. Genes & Development. 30 (21), 2443-2458 (2016).","journal-title":"Genes & Development"},{"key":"ref45","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0246989","type":"journal-article","article-title":"Anti-epileptic drug topiramate upregulates TGF\u03b21 and SOX9 expression in primary embryonic palatal mesenchyme cells: Implications for teratogenicity","author":"Rafi","year":"2021","unstructured":"Rafi, S. K. et al. Anti-epileptic drug topiramate upregulates TGF\u03b21 and SOX9 expression in primary embryonic palatal mesenchyme cells: Implications for teratogenicity. PLoS ONE. In Press (2021).","journal-title":"PLoS ONE"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/62151\/isolation-time-lapse-imaging-primary-mouse-embryonic-palatal","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:01:15Z","timestamp":1792620075000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/62151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,13]]},"references-count":45,"journal-issue":{"issue":"168","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/62151","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,13]]}}}