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Available: https:\/\/www.mouser.de\/datasheet\/2\/228\/5412893-LED_2520Engin_Datasheet_LuxiGen_LZ4-00B208- 1531969.pdf. [Accessed 21 11 2020]. (2020)."},{"key":"ref21","volume-title":"thorlabs.com, thorlabs, [Online]","year":"2020","unstructured":"thorlabs.com, thorlabs, [Online]. Available: https:\/\/www.thorlabs.com\/_sd.cfm?fileName=25135-S01.pdf&partNumber=M625L3. [Accessed on 21 11 2020]. (2020)."},{"issue":"10","key":"ref22","doi-asserted-by":"crossref","first-page":"3894","DOI":"10.1172\/JCI88950","type":"journal-article","article-title":"Optogenetic defibrillation terminates ventricular arrhythmia in mouse hearts and human simulations","volume":"126","author":"Bruegmann","year":"2016","unstructured":"Bruegmann, T. et al. Optogenetic defibrillation terminates ventricular arrhythmia in mouse hearts and human simulations. 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Wiley Interdisciplinary Reviews in System Biology and Medicine. 12 (3), e1477 (2020).","journal-title":"Wiley Interdisciplinary Reviews in System Biology and Medicine"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1093\/cvr\/cvu179","type":"journal-article","article-title":"Light-induced termination of spiral wave arrhythmias by optogenetic engineering of atrial cardiomyocytes","volume":"104","author":"Bingen","year":"2014","unstructured":"Bingen, B. et al. Light-induced termination of spiral wave arrhythmias by optogenetic engineering of atrial cardiomyocytes. Cardiovascular Research. 104 (1), 194-205 (2014).","journal-title":"Cardiovascular Research"},{"issue":"12","key":"ref33","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nphoton.2015.196","type":"journal-article","article-title":"Optical control of excitation waves in cardiac tissue","volume":"9","author":"Burton","year":"2015","unstructured":"Burton, R. A. B. et al. Optical control of excitation waves in cardiac tissue. Nature Photonics. 9 (12), 813-816 (2015).","journal-title":"Nature Photonics"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3389\/fphys.2014.00337","type":"journal-article","article-title":"Toward panoramic in situ mapping of action potential propagation in transgenic hearts to investigate initiation and therapeutic control of arrhythmias","volume":"5","author":"Dura","year":"2014","unstructured":"Dura, M., Schr\u00f6der-Schetelig, J., Luther, S., Lehnart, S. E. Toward panoramic in situ mapping of action potential propagation in transgenic hearts to investigate initiation and therapeutic control of arrhythmias. Frontiers in Physiology. 5, 337 (2014).","journal-title":"Frontiers in Physiology"},{"issue":"35628","key":"ref35","type":"journal-article","article-title":"Optogenetics design of mechanistically-based stimulation patterns for cardiac defibrillation","volume":"6","author":"Crocini","year":"2016","unstructured":"Crocini, C. et al. Optogenetics design of mechanistically-based stimulation patterns for cardiac defibrillation. Science Reports. 6 (35628) (2016).","journal-title":"Science Reports"},{"issue":"27","key":"ref36","first-page":"2132","type":"journal-article","article-title":"Optogenetic termination of ventricular arrhythmias in the whole heart: towards biological cardiac rhythm management","volume":"38","author":"Nyns","year":"2017","unstructured":"Nyns, E. C. A. et al. Optogenetic termination of ventricular arrhythmias in the whole heart: towards biological cardiac rhythm management. European Heart Journal. 38 (27), 2132-2136 (2017).","journal-title":"European Heart Journal"},{"issue":"4","key":"ref37","first-page":"35","type":"journal-article","article-title":"K+atp channel opening and arrhythmogenesis","volume":"24","author":"Wilde","year":"1994","unstructured":"Wilde, A. A. K+atp channel opening and arrhythmogenesis. Journal of Cardiovascular Pharmacology. 24(4), 35-40 (1994).","journal-title":"Journal of Cardiovascular Pharmacology"},{"issue":"1483","key":"ref38","type":"journal-article","article-title":"Marker-free tracking for motion artifact compensation and deformation measurements in optical mapping videos of contracting hearts","volume":"9","author":"Christoph","year":"2018","unstructured":"Christoph, J., Luther, S. Marker-free tracking for motion artifact compensation and deformation measurements in optical mapping videos of contracting hearts. 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