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Epithelial NOTCH signaling rewires the tumor microenvironment of colorectal cancer to drive poor-prognosis subtypes and metastasis. Cancer Cell. 36 (3), 319-336 e317 (2019).","journal-title":"Cancer Cell"},{"issue":"8","key":"ref52","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1038\/s43018-020-0102-y","type":"journal-article","article-title":"Applications of organoids for cancer biology and precision medicine","volume":"1","author":"Lo","year":"2020","unstructured":"Lo, Y.-H., Karlsson, K., Kuo, C. J. Applications of organoids for cancer biology and precision medicine. Nature Cancer. 1 (8), 761-773 (2020).","journal-title":"Nature Cancer"},{"issue":"4","key":"ref53","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.cell.2015.03.053","type":"journal-article","article-title":"Prospective derivation of a living organoid biobank of colorectal cancer patients","volume":"161","author":"van de Wetering","year":"2015","unstructured":"van de Wetering, M. et al. 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