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Nucleic Acids Research. 47 (10), 4974-4985 (2019).","journal-title":"Nucleic Acids Research"},{"issue":"3","key":"ref35","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/nchembio.304","type":"journal-article","article-title":"Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin","volume":"6","author":"Schneider-Poetsch","year":"2010","unstructured":"Schneider-Poetsch, T. et al. Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nature Chemical Biology. 6 (3), 209-217 (2010).","journal-title":"Nature Chemical Biology"},{"issue":"44","key":"ref36","doi-asserted-by":"crossref","first-page":"26404","DOI":"10.1074\/jbc.R115.651679","type":"journal-article","article-title":"Formaldehyde crosslinking: A tool for the study of chromatin complexes","volume":"290","author":"Hoffman","year":"2015","unstructured":"Hoffman, E. A., Frey, B. L., Smith, L. M., Auble, D. T. 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Journal of Experimental Botany. 71 (18), 5323-5332 (2020).","journal-title":"Journal of Experimental Botany"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1038\/nprot.2016.189","type":"journal-article","article-title":"Translation complex profile sequencing to study the in vivo dynamics of mRNA-ribosome interactions during translation initiation, elongation and termination","volume":"12","author":"Shirokikh","year":"2017","unstructured":"Shirokikh, N. E., Archer, S. K., Beilharz, T. H., Powell, D., Preiss, T. Translation complex profile sequencing to study the in vivo dynamics of mRNA-ribosome interactions during translation initiation, elongation and termination. Nature Protocols. 12 (4), 697-731 (2017).","journal-title":"Nature Protocols"},{"issue":"4","key":"ref39","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.molcel.2020.06.004","type":"journal-article","article-title":"Selective translation complex profiling reveals staged initiation and co-translational assembly of initiation factor complexes","volume":"79","author":"Wagner","year":"2020","unstructured":"Wagner, S. et al. Selective translation complex profiling reveals staged initiation and co-translational assembly of initiation factor complexes. Molecular Cell. 79 (4), 546-560.e7 (2020).","journal-title":"Molecular Cell"},{"issue":"9","key":"ref40","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1038\/nrm.2016.109","type":"journal-article","article-title":"Profiling ribosome dynamics","volume":"17","author":"Zlotorynski","year":"2016","unstructured":"Zlotorynski, E. Profiling ribosome dynamics. Nature Reviews Molecular Cell Biology. 17 (9), 535-535 (2016).","journal-title":"Nature Reviews Molecular Cell Biology"},{"issue":"16","key":"ref41","first-page":"8785","type":"journal-article","article-title":"Functional interplay between DEAD-box RNA helicases Ded1 and Dbp1 in preinitiation complex attachment and scanning on structured mRNAs in vivo","volume":"47","author":"Sen","year":"2019","unstructured":"Sen, N. D., Gupta, N., S, K. A., Preiss, T., Lorsch, J. R., Hinnebusch, A. G. Functional interplay between DEAD-box RNA helicases Ded1 and Dbp1 in preinitiation complex attachment and scanning on structured mRNAs in vivo. Nucleic Acids Research. 47 (16), 8785-8806 (2019).","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"20010212","DOI":"10.3390\/ijms20010212","type":"journal-article","article-title":"Translatomics: The global view of translation","volume":"20","author":"Zhao","year":"2019","unstructured":"Zhao, J., Qin, B., Nikolay, R., Spahn, C. M. T., Zhang, G. Translatomics: The global view of translation. International Journal of Molecular Sciences. 20 (1), e20010212 (2019).","journal-title":"International Journal of Molecular Sciences"},{"issue":"1","key":"ref43","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/0003-2697(83)90755-8","type":"journal-article","article-title":"A simple technique for the preparation and storage of sucrose gradients","volume":"135","author":"Luthe","year":"1983","unstructured":"Luthe, D. S., A simple technique for the preparation and storage of sucrose gradients. Analytical Biochemistry. 135 (1), 230-232 (1983).","journal-title":"Analytical Biochemistry"},{"issue":"1","key":"ref44","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nrg2484","type":"journal-article","article-title":"RNA-Seq: A revolutionary tool for transcriptomics","volume":"10","author":"Wang","year":"2009","unstructured":"Wang, Z., Gerstein, M., Snyder, M. RNA-Seq: A revolutionary tool for transcriptomics. Nature Review Genetics. 10(1), 57-63 (2009).","journal-title":"Nature Review Genetics"},{"issue":"3","key":"ref45","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0968-0004(99)01535-2","type":"journal-article","article-title":"Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation","volume":"25","author":"Orlando","year":"2000","unstructured":"Orlando, V. Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation. Trends in Biochemical Sciences. 25 (3), 99-104 (2000).","journal-title":"Trends in Biochemical Sciences"},{"issue":"2","key":"ref46","doi-asserted-by":"crossref","first-page":"4636","DOI":"10.1371\/journal.pone.0004636","type":"journal-article","article-title":"A Temporal Threshold for Formaldehyde Crosslinking and Fixation","volume":"4","author":"Schmiedeberg","year":"2009","unstructured":"Schmiedeberg, L., Skene, P., Deaton, A., Bird, A. A Temporal Threshold for Formaldehyde Crosslinking and Fixation. PLoS One. 4 (2), e4636 (2009).","journal-title":"PLoS One"},{"issue":"19","key":"ref47","doi-asserted-by":"crossref","first-page":"6470","DOI":"10.1073\/pnas.82.19.6470","type":"journal-article","article-title":"Formaldehyde-mediated DNA-protein crosslinking: A probe for in vivo chromatin structures","volume":"82","author":"Solomon","year":"1985","unstructured":"Solomon, M. J., Varshavsky, A. Formaldehyde-mediated DNA-protein crosslinking: A probe for in vivo chromatin structures. Proceedings of the National Academy of Sciences. 82 (19), 6470-6474 (1985).","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"6","key":"ref48","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/S0092-8674(88)90469-2","type":"journal-article","article-title":"Mapping proteinDNA interactions in vivo with formaldehyde: Evidence that histone H4 is retained on a highly transcribed gene","volume":"53","author":"Solomon","year":"1988","unstructured":"Solomon, M. J., Larsen, P. L., Varshavsky, A. Mapping proteinDNA interactions in vivo with formaldehyde: Evidence that histone H4 is retained on a highly transcribed gene. Cell. 53 (6), 937-947 (1988).","journal-title":"Cell"},{"issue":"4","key":"ref49","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.molcel.2020.06.005","type":"journal-article","article-title":"Selective 40S footprinting reveals cap-tethered ribosome scanning in human cells","volume":"79","author":"Bohlen","year":"2020","unstructured":"Bohlen, J., Fenzl, K., Kramer, G., Bukau, B., Teleman, A. A. Selective 40S footprinting reveals cap-tethered ribosome scanning in human cells. Molecular Cell. 79 (4), 561-574.e5 (2020).","journal-title":"Molecular Cell"},{"key":"ref50","type":"journal-article","article-title":"Translation complex stabilization on messenger RNA and footprint profiling to study the RNA responses and dynamics of protein biosynthesis in the cells","author":"Shirokikh","year":"2021","unstructured":"Shirokikh, N.E. Translation complex stabilization on messenger RNA and footprint profiling to study the RNA responses and dynamics of protein biosynthesis in the cells. Critical Reviews in Biochemistry and Molecular Biology. (2021).","journal-title":"Critical Reviews in Biochemistry and Molecular Biology"},{"issue":"3","key":"ref51","doi-asserted-by":"crossref","first-page":"107534","DOI":"10.1016\/j.celrep.2020.107534","type":"journal-article","article-title":"Profiling of small ribosomal subunits reveals modes and regulation of translation initiation","volume":"31","author":"Giess","year":"2020","unstructured":"Giess, A. et al. Profiling of small ribosomal subunits reveals modes and regulation of translation initiation. Cell Reports. 31 (3), 107534 (2020).","journal-title":"Cell Reports"},{"issue":"7","key":"ref52","type":"journal-article","article-title":"Separation and paired proteome profiling of plant chloroplast and cytoplasmic ribosomes","volume":"9","author":"Firmino","year":"2020","unstructured":"Firmino, A. A. P. et al. Separation and paired proteome profiling of plant chloroplast and cytoplasmic ribosomes. Plants (Basel). 9 (7), (2020).","journal-title":"Plants (Basel)"},{"issue":"17","key":"ref53","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1093\/nar\/gku671","type":"journal-article","article-title":"Translation inhibitors cause abnormalities in ribosome profiling experiments","volume":"42","author":"Gerashchenko","year":"2014","unstructured":"Gerashchenko, M.V., Gladyshev, V. N. Translation inhibitors cause abnormalities in ribosome profiling experiments. Nucleic Acids Research. 42 (17), e134 (2014).","journal-title":"Nucleic Acids Research"},{"issue":"10","key":"ref54","doi-asserted-by":"crossref","first-page":"4974","DOI":"10.1093\/nar\/gkz205","type":"journal-article","article-title":"Cycloheximide can distort measurements of mRNA levels and translation efficiency","volume":"47","author":"Santos","year":"2019","unstructured":"Santos, D.A., Shi, L., Tu, B. P., Weissman, J.S. Cycloheximide can distort measurements of mRNA levels and translation efficiency. Nucleic Acids Research. 47 (10), 4974-4985 (2019).","journal-title":"Nucleic Acids Research"},{"issue":"3","key":"ref55","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/nchembio.304","type":"journal-article","article-title":"Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin","volume":"6","author":"Schneider-Poetsch","year":"2010","unstructured":"Schneider-Poetsch, T. et al. Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nature Chemical Biology. 6 (3), 209-217 (2010).","journal-title":"Nature Chemical Biology"},{"issue":"1","key":"ref56","first-page":"29","type":"journal-article","article-title":"Formaldehyde fixation in the third millennium","volume":"21","author":"Pl\u00e9nat","year":"2001","unstructured":"Pl\u00e9nat, F. et al. Formaldehyde fixation in the third millennium. Annales De Pathologie. 21 (1), 29-47 (2001).","journal-title":"Annales De Pathologie"},{"issue":"7","key":"ref57","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1073\/pnas.0712168105","type":"journal-article","article-title":"A chemical method for fast and sensitive detection of DNA synthesis in vivo","volume":"105","author":"Salic","year":"2008","unstructured":"Salic, A., Mitchison, T. J. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proceedings of the National Academy of Sciences. 105 (7), 2415-2420 (2008).","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"7","key":"ref58","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/S0046-8177(87)80243-5","type":"journal-article","article-title":"The formaldehyde-fixed and paraffin-embedded tissues for diagnostic transmission electron microscopy: A retrospective and prospective study","volume":"18","author":"Wang","year":"1987","unstructured":"Wang, N. S., Minassian, H. The formaldehyde-fixed and paraffin-embedded tissues for diagnostic transmission electron microscopy: A retrospective and prospective study. Human Pathology. 18 (7), 715-727 (1987).","journal-title":"Human Pathology"},{"issue":"9","key":"ref59","doi-asserted-by":"crossref","first-page":"5246","DOI":"10.1016\/S0021-9258(18)34662-3","type":"journal-article","article-title":"Characterization of eukaryotic initiation factor 4A, a protein involved in ATP-dependent binding of globin mRNA","volume":"257","author":"Grifo","year":"1982","unstructured":"Grifo, J. A. et al. Characterization of eukaryotic initiation factor 4A, a protein involved in ATP-dependent binding of globin mRNA. Journal of Biological Chemistry. 257 (9), 5246-5252 (1982).","journal-title":"Journal of Biological Chemistry"},{"issue":"2","key":"ref60","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1042\/BA20090273","type":"journal-article","article-title":"Commonly used tag combinations for tandem affinity purification","volume":"55","author":"Li","year":"2010","unstructured":"Li, Y. Commonly used tag combinations for tandem affinity purification. Biotechnology and Applied Biochemistry. 55 (2), 73-83 (2010).","journal-title":"Biotechnology and Applied Biochemistry"},{"issue":"16","key":"ref61","doi-asserted-by":"crossref","first-page":"7664","DOI":"10.1073\/pnas.89.16.7664","type":"journal-article","article-title":"ATP hydrolysis by initiation factor 4A is required for translation initiation in Saccharomyces cerevisiae","volume":"89","author":"Blum","year":"1992","unstructured":"Blum, S. et al. ATP hydrolysis by initiation factor 4A is required for translation initiation in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences. 89 (16), 7664-7668 (1992).","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"40","key":"ref62","doi-asserted-by":"crossref","first-page":"24091","DOI":"10.1074\/jbc.R115.675280","type":"journal-article","article-title":"eIF4F: A Retrospective","volume":"290","author":"Merrick","year":"2015","unstructured":"Merrick, W. C. eIF4F: A Retrospective. Journal of Biological Chemistry. 290 (40), 24091-24099 (2015).","journal-title":"Journal of Biological Chemistry"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0079-6603(02)72073-4","type":"journal-article","article-title":"eIF4A: The godfather of the DEAD box helicases","volume":"72","author":"Rogers","year":"2002","unstructured":"Rogers, G. W., Komar, A. A., Merrick, W. C. eIF4A: The godfather of the DEAD box helicases. 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