{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:39:22Z","timestamp":1792618762397,"version":"4.5.0"},"reference-count":23,"publisher":"MyJove Corporation","issue":"174","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/62819","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T20:00:22Z","timestamp":1629748822000},"source":"Crossref","is-referenced-by-count":0,"title":["Synthetic Antigen Controls for Immunohistochemistry"],"prefix":"10.3791","author":[{"given":"Charles A.","family":"Havnar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy J.","family":"H\u00f6tzel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmina M.","family":"Espiritu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda K.","family":"Rangell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franklin V.","family":"Peale","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/PAI.0000000000000163","type":"journal-article","article-title":"Standardization of positive controls in diagnostic immunohistochemistry: recommendations from the International Ad Hoc Expert Committee","volume":"23","author":"Torlakovic","year":"2015","unstructured":"Torlakovic, E. E. et al. Standardization of positive controls in diagnostic immunohistochemistry: recommendations from the International Ad Hoc Expert Committee. Applied Immunohistochemistry and Molecular Morphology. 23 (1), 1-18 (2015).","journal-title":"Applied Immunohistochemistry and Molecular Morphology"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1369\/0022155419832002","type":"journal-article","article-title":"Synthetic antigen gels as practical controls for standardized and quantitative immunohistochemistry","volume":"67","author":"H\u00f6tzel","year":"2019","unstructured":"H\u00f6tzel, K. J. et al. Synthetic antigen gels as practical controls for standardized and quantitative immunohistochemistry. Journal of Histochemistry and Cytochemistry. 67 (5), 309-334 (2019).","journal-title":"Journal of Histochemistry and Cytochemistry"},{"issue":"10","key":"ref3","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1369\/0022155414545224","type":"journal-article","article-title":"Controls for immunohistochemistry: The histochemical society's standards of practice for validation of immunohistochemical assays","volume":"62","author":"Hewitt","year":"2014","unstructured":"Hewitt, S. M., Baskin, D. G., Frevert, C. W., Stahl, W. L., Rosa-Molinar, E. Controls for immunohistochemistry: The histochemical society's standards of practice for validation of immunohistochemical assays. Journal of Histochemistry and Cytochemistry. 62 (10), 693-697 (2014).","journal-title":"Journal of Histochemistry and Cytochemistry"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1097\/PAI.0000000000000069","type":"journal-article","article-title":"Standardization of negative controls in diagnostic immunohistochemistry: recommendations from the international ad hoc expert panel","volume":"22","author":"Torlakovic","year":"2014","unstructured":"Torlakovic, E.E. et al. Standardization of negative controls in diagnostic immunohistochemistry: recommendations from the international ad hoc expert panel. Applied Immunohistochemistry and Molecular Morphology. 22 (4), 241-252 (2014).","journal-title":"Applied Immunohistochemistry and Molecular Morphology"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1038\/s41374-019-0295-9","type":"journal-article","article-title":"Quantitative assessment of PD-L1 as an analyte in immunohistochemistry diagnostic assays using a standardized cell line tissue microarray","volume":"100","author":"Martinez-Morilla","year":"2020","unstructured":"Martinez-Morilla, S. et al. Quantitative assessment of PD-L1 as an analyte in immunohistochemistry diagnostic assays using a standardized cell line tissue microarray. Laboratory Investigation. 100, 4-15 (2020).","journal-title":"Laboratory Investigation"},{"issue":"7718","key":"ref6","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1038\/s41586-018-0409-3","type":"journal-article","article-title":"Genetic and transcriptional evolution alters cancer cell line drug response","volume":"560","author":"Ben-David","year":"2018","unstructured":"Ben-David, U. et al. Genetic and transcriptional evolution alters cancer cell line drug response. Nature. 560 (7718), 325-330 (2018).","journal-title":"Nature"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1093\/clinchem\/48.3.410","type":"journal-article","article-title":"Synthetic peptides identified from phage-displayed combinatorial libraries as immunodiagnostic assay surrogate quality-control targets","volume":"48","author":"Sompuram","year":"2002","unstructured":"Sompuram, S. R. et al. Synthetic peptides identified from phage-displayed combinatorial libraries as immunodiagnostic assay surrogate quality-control targets. Clinical Chemistry. 48 (3), 410-420 (2002).","journal-title":"Clinical Chemistry"},{"issue":"11","key":"ref8","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1177\/002215540205001101","type":"journal-article","article-title":"A novel quality control slide for quantitative immunohistochemistry testing","volume":"50","author":"Sompuram","year":"2002","unstructured":"Sompuram, S. R. et al. A novel quality control slide for quantitative immunohistochemistry testing. Journal of Histochemistry and Cytochemistry. 50 (11), 1425-1433 (2002).","journal-title":"Journal of Histochemistry and Cytochemistry"},{"issue":"9","key":"ref9","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1369\/0022155415588109","type":"journal-article","article-title":"Standardizing immunohistochemistry: A new reference control for detecting staining problems","volume":"63","author":"Sompuram","year":"2015","unstructured":"Sompuram, S. R., Vani, K., Tracey, B., Kamstock, D. A., Bogen, S. A. Standardizing immunohistochemistry: A new reference control for detecting staining problems. Journal of Histochemistry and Cytochemistry. 63 (9), 681-690 (2015).","journal-title":"Journal of Histochemistry and Cytochemistry"},{"issue":"10","key":"ref10","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1097\/PAI.0000000000000260","type":"journal-article","article-title":"Levey-Jennings analysis uncovers unsuspected causes of immunohistochemistry stain variability","volume":"24","author":"Vani","year":"2016","unstructured":"Vani, K. et al. Levey-Jennings analysis uncovers unsuspected causes of immunohistochemistry stain variability. Applied Immunohistochemistry and Molecular Morphology. 24 (10), 688-694 (2016).","journal-title":"Applied Immunohistochemistry and Molecular Morphology"},{"key":"ref11","first-page":"177","type":"journal-article","article-title":"Evaluation of immunofluorescence with artificial sections of selected antigenicity","volume":"22","author":"Brandtzaeg","year":"1972","unstructured":"Brandtzaeg, P. Evaluation of immunofluorescence with artificial sections of selected antigenicity. Immunology. 22, 177-183 (1972).","journal-title":"Immunology"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/BF00493380","type":"journal-article","article-title":"Monoclonal antibodies demonstrating GABA-Iike immunoreactivity","volume":"86","author":"Matute","year":"1986","unstructured":"Matute, C., Streit, P. Monoclonal antibodies demonstrating GABA-Iike immunoreactivity. Histochemistry. 86, 147-157 (1986).","journal-title":"Histochemistry"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/BF00247039","type":"journal-article","article-title":"Postembedding light- and electron microscopic immunocytochemistry of amino acids: description of a new model system allowing identical conditions for specificity testing and tissue processing","volume":"69","author":"Ottersen","year":"1987","unstructured":"Ottersen, O. P. Postembedding light- and electron microscopic immunocytochemistry of amino acids: description of a new model system allowing identical conditions for specificity testing and tissue processing. Experimental Brain Research. 69, 167-174 (1987).","journal-title":"Experimental Brain Research"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1038\/labinvest.3700596","type":"journal-article","article-title":"'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues","volume":"87","author":"Fowler","year":"2007","unstructured":"Fowler, C. B., Cunningham, R. E., O'Leary, T. J., Mason, J. T. 'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues. Laboratory Investigation. 87, 836-846 (2007).","journal-title":"Laboratory Investigation"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1039\/C7BM00820A","type":"journal-article","article-title":"Serum albumin hydrogels in broad pH and temperature ranges: characterization of their self-assembled structures and nanoscopic and macroscopic properties","volume":"6","author":"Arabi","year":"2018","unstructured":"Arabi, S. H. et al. Serum albumin hydrogels in broad pH and temperature ranges: characterization of their self-assembled structures and nanoscopic and macroscopic properties. Biomaterials Science. 6 (3), 478-492 (2018).","journal-title":"Biomaterials Science"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1111\/j.1365-2559.2011.04034.x","type":"journal-article","article-title":"Human epidermal growth factor receptor 2 (HER2) immunoreactivity: specificity of three pharmacodiagnostic antibodies","volume":"59","author":"Schrohl","year":"2011","unstructured":"Schrohl, A.-S., Pedersen, H. C., Jensen, S. S., Nielsen, S. L., Br\u00fcnner, N. Human epidermal growth factor receptor 2 (HER2) immunoreactivity: speci\ufb01city of three pharmacodiagnostic antibodies. Histopathology. 59, 975-983 (2011).","journal-title":"Histopathology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s10822-016-9902-7","type":"journal-article","article-title":"Pep-Calc.com: a set of web utilities for the calculation of peptide and peptoid properties and automatic mass spectral peak assignment","volume":"30","author":"Lear","year":"2016","unstructured":"Lear, S., Cobb, S. L. Pep-Calc.com: a set of web utilities for the calculation of peptide and peptoid properties and automatic mass spectral peak assignment. Journal of Computer-Aided Molecular Design. 30, 271-277 (2016).","journal-title":"Journal of Computer-Aided Molecular Design"},{"issue":"11","key":"ref18","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1038\/nm791","type":"journal-article","article-title":"Automated subcellular localization and quantification of protein expression in tissue microarrays","volume":"8","author":"Camp","year":"2002","unstructured":"Camp, R. L., Chung, G. G., Rimm, D. L. Automated subcellular localization and quantification of protein expression in tissue microarrays. Nature Medicine. 8 (11), 1323-1327 (2002).","journal-title":"Nature Medicine"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1038\/nm.3488","type":"journal-article","article-title":"Multiplexed ion beam imaging of human breast tumors","volume":"20","author":"Angelo","year":"2014","unstructured":"Angelo, M. et al. Multiplexed ion beam imaging of human breast tumors. Nature Medicine. 20 (4), 436-442 (2014).","journal-title":"Nature Medicine"},{"issue":"12","key":"ref20","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1074\/mcp.M112.020800","type":"journal-article","article-title":"High-resolution mapping of linear antibody epitopes using ultrahigh-density peptide microarrays","volume":"11","author":"Buus","year":"2012","unstructured":"Buus, S. et al. High-resolution mapping of linear antibody epitopes using ultrahigh-density peptide microarrays. Molecular and Cellular Proteomics. 11 (12), 1790-1800 (2012).","journal-title":"Molecular and Cellular Proteomics"},{"issue":"6","key":"ref21","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1074\/mcp.M113.033308","type":"journal-article","article-title":"Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays","volume":"13","author":"Forsstr\u00f6m","year":"2014","unstructured":"Forsstr\u00f6m, B. et al. Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays. Molecular and Cellular Proteomics. 13 (6), 1585-1597 (2014).","journal-title":"Molecular and Cellular Proteomics"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"0121673","DOI":"10.1371\/journal.pone.0121673","type":"journal-article","article-title":"Dissecting antibodies with regards to linear and conformational epitopes","volume":"10","author":"Forsstr\u00f6m","year":"2015","unstructured":"Forsstr\u00f6m, B. et al. Dissecting antibodies with regards to linear and conformational epitopes. PLoS One. 10 (3), e0121673 (2015).","journal-title":"PLoS One"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1002\/cpt.1537","type":"journal-article","article-title":"Toward a consensus on applying quantitative liquid chromatography-tandem mass spectrometry proteomics in translational pharmacology research: A white paper","volume":"106","author":"Prasad","year":"2019","unstructured":"Prasad, B. et al. Toward a consensus on applying quantitative liquid chromatography-tandem mass spectrometry proteomics in translational pharmacology research: A white paper. Clinical Pharmacology and Therapeutics. 106 (3), 525-543 (2019).","journal-title":"Clinical Pharmacology and Therapeutics"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/62819\/synthetic-antigen-controls-for-immunohistochemistry","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:05:19Z","timestamp":1792616719000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/62819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,23]]},"references-count":23,"journal-issue":{"issue":"174","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/62819","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,23]]}}}