{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:38:46Z","timestamp":1792618726225,"version":"4.5.0"},"reference-count":20,"publisher":"MyJove Corporation","issue":"176","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/62947","type":"journal-article","created":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T20:06:41Z","timestamp":1633637201000},"source":"Crossref","is-referenced-by-count":1,"title":["Kinetx: A Combined Flow Cytometry Assay and Analysis Software Framework to Quantitatively Measure and Categorize Platelet Activation in Real-time."],"prefix":"10.3791","author":[{"given":"Joanne L.","family":"Dunster","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo L.","family":"Mitchell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin M. M.","family":"Mohammed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk A.","family":"Taylor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan M.","family":"Gibbins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris I.","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2021,10,7]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1111\/jth.13302","type":"journal-article","article-title":"Platelet signaling: a complex interplay between inhibitory and activatory networks","volume":"14","author":"Bye","year":"2016","unstructured":"Bye, A. P., Unsworth, A. J., Gibbins, J. M. Platelet signaling: a complex interplay between inhibitory and activatory networks. Journal of Thrombosis and Haemostasis: JTH. 14 (5), 918-930 (2016).","journal-title":"Journal of Thrombosis and Haemostasis: JTH"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/978-1-61779-307-3_23","type":"journal-article","article-title":"Endogenous inhibitory mechanisms and the regulation of platelet function","volume":"788","author":"Jones","year":"2012","unstructured":"Jones, C. I., Barrett, N. E., Moraes, L. A., Gibbins, J. M., Jackson, D. E. Endogenous inhibitory mechanisms and the regulation of platelet function. Methods in Molecular Biology. 788., 341-366 (2012).","journal-title":"Methods in Molecular Biology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"133","DOI":"10.2147\/VHRM.S44469","type":"journal-article","article-title":"Platelet function tests: a comparative review","volume":"11","author":"Paniccia","year":"2015","unstructured":"Paniccia, R., Priora, R., Liotta, A. A., Abbate, R. Platelet function tests: a comparative review. Vascular Health and Risk Management. 11., 133-148 (2015).","journal-title":"Vascular Health and Risk Management"},{"issue":"2","key":"ref4","first-page":"62","type":"journal-article","article-title":"New horizons in platelets flow cytometry","volume":"20","author":"Saboor","year":"2013","unstructured":"Saboor, M., Moinuddin, M., Ilyas, S. New horizons in platelets flow cytometry. The Malaysian Journal of Medical Sciences : MJMS. 20 (2), 62-66 (2013).","journal-title":"The Malaysian Journal of Medical Sciences : MJMS"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1182\/blood.V88.5.1525.1525","type":"journal-article","article-title":"Platelets and shear stress","volume":"88","author":"Kroll","year":"1996","unstructured":"Kroll, M. H., Hellums, J. D., McIntire, L. V., Schafer, A. I., Moake, J. L. Platelets and shear stress. Blood. 88 (5), 1525-1541 (1996).","journal-title":"Blood"},{"issue":"1","key":"ref6","first-page":"115","type":"journal-article","article-title":"Evidence that the platelet integrin alphaIIb beta3 is regulated by the integrin-linked kinase, ILK, in a PI3-kinase dependent pathway","volume":"88","author":"Pasquet","year":"2002","unstructured":"Pasquet, J. M., Noury, M., Nurden, A. T. Evidence that the platelet integrin alphaIIb beta3 is regulated by the integrin-linked kinase, ILK, in a PI3-kinase dependent pathway. Thrombosis and Haemostasis. 88 (1), 115-122 (2002).","journal-title":"Thrombosis and Haemostasis"},{"issue":"8","key":"ref7","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1111\/j.1538-7836.2004.00870.x","type":"journal-article","article-title":"The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha 2 beta 1","volume":"2","author":"Stevens","year":"2004","unstructured":"Stevens, J. M., Jordan, P. A., Sage, T., Gibbins, J. M. The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha 2 beta 1. Journal of Thrombosis and Haemostasis: JTH. 2 (8), 1443-1452 (2004).","journal-title":"Journal of Thrombosis and Haemostasis: JTH"},{"issue":"8","key":"ref8","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1111\/jth.12620","type":"journal-article","article-title":"Integrin-linked kinase regulates the rate of platelet activation and is essential for the formation of stable thrombi","volume":"12","author":"Jones","year":"2014","unstructured":"Jones, C. I. et al. Integrin-linked kinase regulates the rate of platelet activation and is essential for the formation of stable thrombi. Journal of Thrombosis and Haemostasis: JTH. 12 (8), 1342-1352 (2014).","journal-title":"Journal of Thrombosis and Haemostasis: JTH"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1182\/blood-2008-02-139733","type":"journal-article","article-title":"CalDAG-GEFI and protein kinase C represent alternative pathways leading to activation of integrin alphaIIbbeta3 in platelets","volume":"112","author":"Cifuni","year":"2008","unstructured":"Cifuni, S. M., Wagner, D. D., Bergmeier, W. CalDAG-GEFI and protein kinase C represent alternative pathways leading to activation of integrin alphaIIbbeta3 in platelets. Blood. 112 (5), 1696-1703 (2008).","journal-title":"Blood"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1182\/blood-2010-07-297713","type":"journal-article","article-title":"The kinetics of alphaIIbbeta3 activation determines the size and stability of thrombi in mice: implications for antiplatelet therapy","volume":"117","author":"Stolla","year":"2011","unstructured":"Stolla, M. et al. The kinetics of alphaIIbbeta3 activation determines the size and stability of thrombi in mice: implications for antiplatelet therapy. Blood. 117 (3), 1005-1013 (2011).","journal-title":"Blood"},{"issue":"22","key":"ref11","doi-asserted-by":"crossref","first-page":"13278","DOI":"10.1073\/pnas.95.22.13278","type":"journal-article","article-title":"A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia","volume":"95","author":"Kawasaki","year":"1998","unstructured":"Kawasaki, H. et al. A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia. Proceedings of the National Academy of Sciences of the United States of America. 95 (22), 13278-13283 (1998).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref12","volume-title":"Kinetx","year":"2021","unstructured":"Kinetx. at <http:\/\/github.com\/cardiomaths\/Kinetx> (2021)."},{"key":"ref13","volume-title":"R","year":"2021","unstructured":"R. at <https:\/\/www.r-project.org\/> (2021)."},{"key":"ref14","volume-title":"R Studio","year":"2021","unstructured":"R Studio. at <https:\/\/rstudio.com\/products\/rstudio\/download\/> (2021)."},{"issue":"8","key":"ref15","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1182\/blood-2005-03-1290","type":"journal-article","article-title":"Aggregometry detects platelet hyperreactivity in healthy individuals","volume":"106","author":"Yee","year":"2005","unstructured":"Yee, D. L, Sun, C. W., Bergeron, A. L., Dong, J. F., Bray, P. F. Aggregometry detects platelet hyperreactivity in healthy individuals. Blood. 106 (8), 2723-2729 (2005).","journal-title":"Blood"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00277-005-0029-5","type":"journal-article","article-title":"Agonists-induced platelet activation varies considerably in healthy male individuals: studies by flow cytometry","volume":"85","author":"Panzer","year":"2006","unstructured":"Panzer, S., Hocker, L., Koren, D. Agonists-induced platelet activation varies considerably in healthy male individuals: studies by flow cytometry. Annals of Hematology. 85 (2), 121-125 (2006).","journal-title":"Annals of Hematology"},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1002\/cyto.a.24017","type":"journal-article","article-title":"Flow cytometric monitoring of dynamic cytosolic calcium, sodium, and potassium fluxes following platelet activation","volume":"97","author":"Aliotta","year":"2020","unstructured":"Aliotta, A., Bertaggia Calderara, D., Alberio, L. Flow cytometric monitoring of dynamic cytosolic calcium, sodium, and potassium fluxes following platelet activation. Cytometry. Part A : The Journal of the International Society for Analytical Cytology. 97 (9), 933-944 (2020).","journal-title":"Cytometry. Part A : The Journal of the International Society for Analytical Cytology"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1002\/(SICI)1097-0320(19990401)35:4<302::AID-CYTO2>3.0.CO;2-J","type":"journal-article","article-title":"Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41","volume":"35","author":"do Ceu Monteiro","year":"1999","unstructured":"do Ceu Monteiro, M., Sansonetty, F., Goncalves, M. J., O'Connor, J. E. Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41. Cytometry. 35 (4), 302-310 (1999).","journal-title":"Cytometry"},{"issue":"11","key":"ref19","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1002\/cyto.a.20974","type":"journal-article","article-title":"A flow-cytometric method for continuous measurement of intracellular Ca(2+) concentration","volume":"77","author":"Vines","year":"2010","unstructured":"Vines, A., McBean, G. J, Blanco-Fernandez, A. A flow-cytometric method for continuous measurement of intracellular Ca(2+) concentration. Cytometry. Part A : The Journal of the International Society for Analytical Cytology. 77 (11), 1091-1097 (2010).","journal-title":"Cytometry. Part A : The Journal of the International Society for Analytical Cytology"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1182\/blood.V98.5.1619","type":"journal-article","article-title":"Differential inhibition of adenosine diphosphate- versus thrombin receptor-activating peptide-stimulated platelet fibrinogen binding by abciximab due to different glycoprotein IIb\/IIIa activation kinetics","volume":"98","author":"Weber","year":"2001","unstructured":"Weber, A. A., Schror, K. Differential inhibition of adenosine diphosphate- versus thrombin receptor-activating peptide-stimulated platelet fibrinogen binding by abciximab due to different glycoprotein IIb\/IIIa activation kinetics. Blood. 98 (5), 1619-1621 (2001).","journal-title":"Blood"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/62947\/kinetx-combined-flow-cytometry-assay-analysis-software-framework-to","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:52:33Z","timestamp":1792615953000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/62947"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,7]]},"references-count":20,"journal-issue":{"issue":"176","published-online":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.3791\/62947","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,7]]}}}