{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:49:16Z","timestamp":1792615756770,"version":"4.5.0"},"reference-count":32,"publisher":"MyJove Corporation","issue":"181","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/63175","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T20:00:50Z","timestamp":1648756850000},"source":"Crossref","is-referenced-by-count":5,"title":["High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening"],"prefix":"10.3791","author":[{"given":"Yu-Fen","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Chi","family":"Su","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chuan","family":"Su","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Wen","family":"Chung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Yi","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ju","family":"Kao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Pin","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Daniels","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2022,3,31]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/scrt169","type":"journal-article","article-title":"The 3R principle: Advancing clinical application of human pluripotent stem cells","volume":"4","author":"O'Connor","year":"2013","unstructured":"O'Connor, M. D. The 3R principle: Advancing clinical application of human pluripotent stem cells. Stem Cell Research & Therapy. 4 (2), 21 (2013).","journal-title":"Stem Cell Research & Therapy"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1097\/FJC.0b013e318247f642","type":"journal-article","article-title":"Induced pluripotent stem cells as a disease modeling and drug screening platform","volume":"60","author":"Ebert","year":"2012","unstructured":"Ebert, A. D., Liang, P., Wu, J. C. Induced pluripotent stem cells as a disease modeling and drug screening platform. Journal of Cardiovascular Pharmacology. 60 (4), 408-416 (2012).","journal-title":"Journal of Cardiovascular Pharmacology"},{"issue":"19","key":"ref3","doi-asserted-by":"crossref","first-page":"7320","DOI":"10.3390\/ijms21197320","type":"journal-article","article-title":"Drug development and the use of induced pluripotent stem cell-derived cardiomyocytes for disease modeling and drug toxicity screening","volume":"21","author":"Ovics","year":"2020","unstructured":"Ovics, P. et al. Drug development and the use of induced pluripotent stem cell-derived cardiomyocytes for disease modeling and drug toxicity screening. International Journal of Molecular Sciences. 21 (19), 7320 (2020).","journal-title":"International Journal of Molecular Sciences"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ymeth.2008.09.025","type":"journal-article","article-title":"Chemical calcium indicators","volume":"46","author":"Paredes","year":"2008","unstructured":"Paredes, R. M., Etzler, J. C., Watts, L. T., Zheng, W., Lechleiter, J. D. Chemical calcium indicators. Methods. 46 (3), 143-151 (2008).","journal-title":"Methods"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1152\/physrev.1999.79.4.1089","type":"journal-article","article-title":"Measurement of intracellular calcium","volume":"79","author":"Takahashi","year":"1999","unstructured":"Takahashi, A., Camacho, P., Lechleiter, J. D., Herman, B. Measurement of intracellular calcium. Physiological Reviews. 79 (4), 1089-1125 (1999).","journal-title":"Physiological Reviews"},{"issue":"515","key":"ref6","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.aal2039","type":"journal-article","article-title":"Fluorescent Ca(2+) indicators directly inhibit the Na,K-ATPase and disrupt cellular functions","volume":"11","author":"Smith","year":"2018","unstructured":"Smith, N. A. et al. Fluorescent Ca(2+) indicators directly inhibit the Na,K-ATPase and disrupt cellular functions. Science Signaling. 11 (515), eaal2039 (2018).","journal-title":"Science Signaling"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12915-018-0480-0","type":"journal-article","article-title":"A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578","volume":"16","author":"Shen","year":"2018","unstructured":"Shen, Y. et al. A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578. BMC Biology. 16 (1), 9 (2018).","journal-title":"BMC Biology"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/s41592-018-0294-6","type":"journal-article","article-title":"A genetically encoded near-infrared fluorescent calcium ion indicator","volume":"16","author":"Qian","year":"2019","unstructured":"Qian, Y. et al. A genetically encoded near-infrared fluorescent calcium ion indicator. Nature Methods. 16 (2), 171-174 (2019).","journal-title":"Nature Methods"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1042\/BJ20140931","type":"journal-article","article-title":"Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum","volume":"464","author":"Wu","year":"2014","unstructured":"Wu, J. et al. Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum. Biochemical Journal. 464 (1), 13-22 (2014).","journal-title":"Biochemical Journal"},{"issue":"7","key":"ref10","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1021\/acssensors.0c00279","type":"journal-article","article-title":"and high-performance genetically encoded Ca2+ indicator based on mneongreen fluorescent protein","volume":"5","author":"Zarowny","year":"2020","unstructured":"Zarowny, L. et al. Bright and high-performance genetically encoded Ca2+ indicator based on mneongreen fluorescent protein. ACS Sensors. 5 (7), 1959-1968 (2020).","journal-title":"ACS Sensors"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"148","DOI":"10.3390\/ijms22010148","type":"journal-article","article-title":"Drug screening with genetically encoded fluorescent sensors: today and tomorrow","volume":"22","author":"Potekhina","year":"2021","unstructured":"Potekhina, E. S. et al. Drug screening with genetically encoded fluorescent sensors: today and tomorrow. International Journal of Molecular Sciences. 22 (1), 148 (2021).","journal-title":"International Journal of Molecular Sciences"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"0174181","DOI":"10.1371\/journal.pone.0174181","type":"journal-article","article-title":"Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics","volume":"12","author":"Chang","year":"2017","unstructured":"Chang, Y.-F. et al. Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics. PLoS One. 12 (4), e0174181 (2017).","journal-title":"PLoS One"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.neures.2012.05.007","type":"journal-article","article-title":"Optogenetic activation during detector &#34;dead time&#34; enables compatible real-time fluorescence imaging","volume":"73","author":"Chang","year":"2012","unstructured":"Chang, Y.-F., Arai, Y., Nagai, T. Optogenetic activation during detector \"dead time\" enables compatible real-time fluorescence imaging. Neuroscience Research. 73 (4), 341-347 (2012).","journal-title":"Neuroscience Research"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1038\/nmeth.1593","type":"journal-article","article-title":"Chemically defined conditions for human iPSC derivation and culture","volume":"8","author":"Chen","year":"2011","unstructured":"Chen, G. et al. Chemically defined conditions for human iPSC derivation and culture. Nature Methods. 8 (5), 424-429 (2011).","journal-title":"Nature Methods"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/0471142735.ima03bs111","type":"journal-article","article-title":"Trypan blue exclusion test of cell viability","volume":"111","author":"Strober","year":"2015","unstructured":"Strober, W. Trypan blue exclusion test of cell viability. Current Protocols in Immunology. 111, A3.B.1-A3.B.3 (2015).","journal-title":"Current Protocols in Immunology"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1039\/C9SM01531K","type":"journal-article","article-title":"Modulating cardiomyocyte and fibroblast interaction using layer-by-layer deposition facilitates synchronisation of cardiac macro tissues","volume":"16","author":"Jang","year":"2020","unstructured":"Jang, Y. et al. Modulating cardiomyocyte and fibroblast interaction using layer-by-layer deposition facilitates synchronisation of cardiac macro tissues. Soft Matter. 16 (2), 428-434 (2020).","journal-title":"Soft Matter"},{"issue":"1","key":"ref17","first-page":"17","type":"journal-article","article-title":"Calcium-channel blocking agents","volume":"1","author":"Leonard","year":"1982","unstructured":"Leonard, R. G., Talbert, R. L. Calcium-channel blocking agents. Clinical Pharmacology. 1 (1), 17-33 (1982).","journal-title":"Clinical Pharmacology"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1085\/jgp.96.1.195","type":"journal-article","article-title":"Two components of cardiac delayed rectifier K+ current. Differential sensitivity to block by class III antiarrhythmic agents","volume":"96","author":"Sanguinetti","year":"1990","unstructured":"Sanguinetti, M. C., Jurkiewicz, N. K. Two components of cardiac delayed rectifier K+ current. Differential sensitivity to block by class III antiarrhythmic agents. The Journal of General Physiology. 96 (1), 195-215 (1990).","journal-title":"The Journal of General Physiology"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0026-895X(24)23209-0","type":"journal-article","article-title":"Molecular determinant of high-affinity dofetilide binding to HERG1 expressed in Xenopus oocytes: Involvement of S6 sites","volume":"57","author":"Lees-Miller","year":"2000","unstructured":"Lees-Miller, J. P., Duan, Y., Teng, G. Q., Duff, H. J. Molecular determinant of high-affinity dofetilide binding to HERG1 expressed in Xenopus oocytes: Involvement of S6 sites. Molecular Pharmacology. 57 (2), 367-374 (2000).","journal-title":"Molecular Pharmacology"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1124\/mol.105.020990","type":"journal-article","article-title":"Molecular determinants of HERG channel block","volume":"69","author":"Kamiya","year":"2006","unstructured":"Kamiya, K., Niwa, R., Mitcheson, J. S., Sanguinetti, M. C. Molecular determinants of HERG channel block. Molecular Pharmacology. 69 (5), 1709-1716 (2006).","journal-title":"Molecular Pharmacology"},{"issue":"11","key":"ref21","doi-asserted-by":"crossref","first-page":"0241287","DOI":"10.1371\/journal.pone.0241287","type":"journal-article","article-title":"Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells","volume":"15","author":"Fukushima","year":"2020","unstructured":"Fukushima, H. et al. Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells. PLoS One. 15 (11), e0241287 (2020).","journal-title":"PLoS One"},{"issue":"6","key":"ref22","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.bpj.2016.04.054","type":"journal-article","article-title":"Genetically encoded fluorescent indicators for organellar calcium imaging","volume":"111","author":"Suzuki","year":"2016","unstructured":"Suzuki, J., Kanemaru, K., Iino, M. Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical Journal. 111 (6), 1119-1131 (2016).","journal-title":"Biophysical Journal"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"4153","DOI":"10.1038\/ncomms5153","type":"journal-article","article-title":"Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA","volume":"5","author":"Suzuki","year":"2014","unstructured":"Suzuki, J. et al. Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA. Nature Communications. 5, 4153 (2014).","journal-title":"Nature Communications"},{"issue":"11","key":"ref24","doi-asserted-by":"crossref","first-page":"3000965","DOI":"10.1371\/journal.pbio.3000965","type":"journal-article","article-title":"Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging","volume":"18","author":"Qian","year":"2020","unstructured":"Qian, Y. et al. Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. PLoS Biology. 18 (11), e3000965 (2020).","journal-title":"PLoS Biology"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1073\/pnas.1523410113","type":"journal-article","article-title":"Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx","volume":"113","author":"Dynes","year":"2016","unstructured":"Dynes, J. L., Amcheslavsky, A., Cahalan, M. D. Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Proceedings of the National Academy of Sciences of the United States of America. 113 (2), 440-445 (2016).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3390\/cells7060051","type":"journal-article","article-title":"Fluorescent, bioluminescent, and optogenetic approaches to study excitable physiology in the single cardiomyocyte","volume":"7","author":"Broyles","year":"2018","unstructured":"Broyles, C. N., Robinson, P., Daniels, M. J. Fluorescent, bioluminescent, and optogenetic approaches to study excitable physiology in the single cardiomyocyte. Cells. 7 (6), 51 (2018).","journal-title":"Cells"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s11307-009-0236-x","type":"journal-article","article-title":"Comparison of gene-transfer efficiency in human embryonic stem cells","volume":"12","author":"Cao","year":"2010","unstructured":"Cao, F. et al. Comparison of gene-transfer efficiency in human embryonic stem cells. Molecular Imaging and Biology. 12 (1), 15-24 (2010).","journal-title":"Molecular Imaging and Biology"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1634\/stemcells.21-1-111","type":"journal-article","article-title":"High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors","volume":"21","author":"Ma","year":"2003","unstructured":"Ma, Y., Ramezani, A., Lewis, R., Hawley, R. G., Thomson, J. A. High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors. Stem Cells. 21 (1), 111-117 (2003).","journal-title":"Stem Cells"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3389\/fnana.2019.00093","type":"journal-article","article-title":"Adeno-associated virus technologies and methods for targeted neuronal manipulation","volume":"13","author":"Haery","year":"2019","unstructured":"Haery, L. et al. Adeno-associated virus technologies and methods for targeted neuronal manipulation. Frontiers in Neuroanatomy. 13, 93 (2019).","journal-title":"Frontiers in Neuroanatomy"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/s41434-020-0169-1","type":"journal-article","article-title":"Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: Comparison of four promoters","volume":"28","author":"Nieuwenhuis","year":"2021","unstructured":"Nieuwenhuis, B. et al. Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy. 28 (1), 56-74 (2021).","journal-title":"Gene Therapy"},{"issue":"23","key":"ref31","doi-asserted-by":"crossref","first-page":"11254","DOI":"10.1128\/JVI.74.23.11254-11261.2000","type":"journal-article","article-title":"Characterization of promoter function and cell-type-specific expression from viral vectors in the nervous system","volume":"74","author":"Smith","year":"2000","unstructured":"Smith, R. L. et al. Characterization of promoter function and cell-type-specific expression from viral vectors in the nervous system. Journal of Virology. 74 (23), 11254-11261 (2000).","journal-title":"Journal of Virology"},{"key":"ref32","first-page":"14","type":"journal-article","article-title":"Safety and efficacy of an immune cell-specific chimeric promoter in regulating anti-PD-1 antibody expression in","volume":"19","author":"Fang","year":"2020","unstructured":"Fang, Y. et al. Safety and efficacy of an immune cell-specific chimeric promoter in regulating anti-PD-1 antibody expression in CAR T cells. Molecular Therapy - Methods & Clinical Development. 19, 14-23 (2020).","journal-title":"CAR T cells. Molecular Therapy - Methods & Clinical Development"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/63175\/high-throughput-optical-controlling-recording-calcium-signal-ipsc","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:09:12Z","timestamp":1792613352000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/63175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,31]]},"references-count":32,"journal-issue":{"issue":"181","published-online":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.3791\/63175","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,31]]}}}