{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:38:50Z","timestamp":1792600730407,"version":"4.5.0"},"reference-count":50,"publisher":"MyJove Corporation","issue":"215","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/63981","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T19:01:14Z","timestamp":1737745274000},"source":"Crossref","is-referenced-by-count":4,"title":["Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization"],"prefix":"10.3791","author":[{"given":"Rupali","family":"Dabas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luka","family":"Blagojevic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazila","family":"Kamaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2025,1,24]]},"reference":[{"issue":"7","key":"ref1","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1039\/C4CS00341A","type":"journal-article","article-title":"Micro- and nanogels with labile crosslinks - from synthesis to biomedical applications","volume":"44","author":"Zhang","year":"2015","unstructured":"Zhang, X., Malhotra, S., Molina, M., Haag, R. Micro- and nanogels with labile crosslinks - from synthesis to biomedical applications. Chem Soc Rev. 44 (7), 1948-1973 (2015).","journal-title":"Chem Soc Rev"},{"issue":"9","key":"ref2","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.addr.2012.02.002","type":"journal-article","article-title":"Polymer nanogels: A versatile nanoscopic drug delivery platform","volume":"64","author":"Chacko","year":"2012","unstructured":"Chacko, R. T., Ventura, J., Zhuang, J., Thayumanavan, S. Polymer nanogels: A versatile nanoscopic drug delivery platform. Adv Drug Del Rev. 64 (9), 836-851, (2012).","journal-title":"Adv Drug Del Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2019.05.018","type":"journal-article","article-title":"Stimulus-responsive polymeric nanogels as smart drug delivery systems","volume":"92","author":"Hajebi","year":"2019","unstructured":"Hajebi, S. et al. Stimulus-responsive polymeric nanogels as smart drug delivery systems. Acta Biomaterialia. 92, 1-18 (2019).","journal-title":"Acta Biomaterialia"},{"issue":"39","key":"ref4","doi-asserted-by":"crossref","first-page":"4509","DOI":"10.1002\/adma.201102371","type":"journal-article","article-title":"Nanogel star polymer architectures: A nanoparticle platform for modular programmable macromolecular self-assembly, intercellular transport, and dual-mode cargo delivery","volume":"23","author":"Lee","year":"2015","unstructured":"Lee, V. Y. et al. Nanogel star polymer architectures: A nanoparticle platform for modular programmable macromolecular self-assembly, intercellular transport, and dual-mode cargo delivery. Adv Mat. 23 (39), 4509-4515 (2015).","journal-title":"Adv Mat"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1039\/C7TB02239E","type":"journal-article","article-title":"Responsive crosslinked polymer nanogels for imaging and therapeutics delivery","volume":"6","author":"Ekkelenkamp","year":"2018","unstructured":"Ekkelenkamp, A., Rach\u00e8l Elzes, M., Engbersen, J. F., Paulusse, J. M. Responsive crosslinked polymer nanogels for imaging and therapeutics delivery. J Mat Chem B. 6 (2), 210-235 (2018).","journal-title":"J Mat Chem B"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jconrel.2005.06.002","type":"journal-article","article-title":"Polyplex nanogel formulations for drug delivery of cytotoxic nucleoside analogs","volume":"107","author":"Vinogradov","year":"2015","unstructured":"Vinogradov, S. V., Zeman, A. D., Batrakova, E. V., Kabanov, A. V. Polyplex nanogel formulations for drug delivery of cytotoxic nucleoside analogs. J Control Rel. 107 (1), 143-157 (2015).","journal-title":"J Control Rel"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"3062","DOI":"10.1021\/ma00064a011","type":"journal-article","article-title":"Self-aggregates of hydrophobized polysaccharides in water. Formation and characteristics of nanoparticles","volume":"26","author":"Akiyoshi","year":"1993","unstructured":"Akiyoshi, K., Deguchi, S., Moriguchi, N., Yamaguchi, S., Sunamoto, J. Self-aggregates of hydrophobized polysaccharides in water. Formation and characteristics of nanoparticles. Macromol. 26 (12), 3062-3068 (1993).","journal-title":"Macromol"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3109\/10611860008996855","type":"journal-article","article-title":"Block and graft copolymers and NanoGel copolymer networks for DNA delivery into cell","volume":"8","author":"Lemieux","year":"2000","unstructured":"Lemieux, P. et al., A. Block and graft copolymers and NanoGel copolymer networks for DNA delivery into cell. J Drug Target. 8 (2), 91-105 (2000).","journal-title":"J Drug Target"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ejpb.2020.10.009","type":"journal-article","article-title":"Recent developments in stimuli-responsive polymer nanogels for drug delivery and diagnostics: A review","volume":"157","author":"Preman","year":"2020","unstructured":"Preman, N. K., Barki, R. R., Vijayan, A., Sanjeeva, S. G., Johnson, R. P. Recent developments in stimuli-responsive polymer nanogels for drug delivery and diagnostics: A review. Euro J Pharma Biopharmac. 157, 121-153 (2020).","journal-title":"Euro J Pharma Biopharmac"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.progpolymsci.2008.01.002","type":"journal-article","article-title":"The development of microgels\/nanogels for drug delivery applications","volume":"33","author":"Oh","year":"2008","unstructured":"Oh, J. K., Drumright, R., Siegwart, D. J., Matyjaszewski, K. The development of microgels\/nanogels for drug delivery applications. Prog Poly Sci. 33 (4), 448-477 (2008).","journal-title":"Prog Poly Sci"},{"issue":"18","key":"ref11","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1016\/j.biomaterials.2014.03.001","type":"journal-article","article-title":"Click hydrogels, microgels and nanogels: Emerging platforms for drug delivery and tissue engineering","volume":"35","author":"Jiang","year":"2014","unstructured":"Jiang, Y., Chen, J., Deng, C., Suuronen, E. J., Zhong, Z. Click hydrogels, microgels and nanogels: Emerging platforms for drug delivery and tissue engineering. Biomat. 35 (18), 4969-4985 (2014).","journal-title":"Biomat"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1080\/15583720701454999","type":"journal-article","article-title":"Nanoparticle drug delivery platform","volume":"47","author":"Napier","year":"2007","unstructured":"Napier, M. E., DeSimone, J. M. Nanoparticle drug delivery platform. Poly Rev. 47 (3), 321-327 (2007).","journal-title":"Poly Rev"},{"issue":"9","key":"ref13","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1039\/b608348j","type":"journal-article","article-title":"Soft drug delivery systems","volume":"2","author":"Malmsten","year":"2006","unstructured":"Malmsten, M. Soft drug delivery systems. Soft Matt. 2 (9), 760-769 (2006).","journal-title":"Soft Matt"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"2602","DOI":"10.1021\/acs.chemrev.5b00346","type":"journal-article","article-title":"Degradable controlled-release polymers and polymeric nanoparticles: Mechanisms of controlling drug release","volume":"116","author":"Kamaly","year":"2016","unstructured":"Kamaly, N., Yameen, B., Wu, J., Farokhzad, O. C. Degradable controlled-release polymers and polymeric nanoparticles: Mechanisms of controlling drug release. Che Rev. 116 (4), 2602-2663 (2016).","journal-title":"Che Rev"},{"issue":"17","key":"ref15","doi-asserted-by":"crossref","first-page":"6161","DOI":"10.1039\/C5CS00199D","type":"journal-article","article-title":"Stimuli-responsive nanogel composites and their application in nanomedicine","volume":"44","author":"Molina","year":"2015","unstructured":"Molina, M. et al. Stimuli-responsive nanogel composites and their application in nanomedicine. Chem Soc Rev. 44 (17), 6161-6186 (2015).","journal-title":"Chem Soc Rev"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/j.msec.2016.11.031","type":"journal-article","article-title":"Advancement of multifunctional hybrid nanogel systems: Construction and application in drug co-delivery and imaging technique","volume":"71","author":"Ma","year":"2017","unstructured":"Ma, Y., Ge, Y., Li, L. Advancement of multifunctional hybrid nanogel systems: Construction and application in drug co-delivery and imaging technique. Mat Sci Eng: C. 71, 1281-1292 (2017).","journal-title":"Mat Sci Eng: C"},{"issue":"34","key":"ref17","doi-asserted-by":"crossref","first-page":"6986","DOI":"10.1039\/C7TB01624G","type":"journal-article","article-title":"Recent advances in engineered chitosan-based nanogels for biomedical applications","volume":"5","author":"Wang","year":"2017","unstructured":"Wang, H., Qian, J., Ding, F. Recent advances in engineered chitosan-based nanogels for biomedical applications. J Mat Chem B. 5 (34), 6986-7007 (2017).","journal-title":"J Mat Chem B"},{"issue":"13","key":"ref18","doi-asserted-by":"crossref","first-page":"1901935","DOI":"10.1002\/adma.201901935","type":"journal-article","article-title":"Oral delivery of biologics for precision medicine","volume":"32","author":"Dur\u00e1n-Lobato","year":"2020","unstructured":"Dur\u00e1n-Lobato, M., Niu, Z., Alonso, M. J. Oral delivery of biologics for precision medicine. Adv Mat. 32 (13), 1901935 (2020).","journal-title":"Adv Mat"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.3390\/molecules28031038","type":"journal-article","article-title":"The pharmaceutical industry in 2022: An analysis of FDA drug approvals from the perspective of molecules","volume":"28","author":"de la Torre","year":"2023","unstructured":"de la Torre, B. G., Albericio, F. The pharmaceutical industry in 2022: An analysis of FDA drug approvals from the perspective of molecules. Molecules. 28 (3), 1038 (2023).","journal-title":"Molecules"},{"key":"ref20","first-page":"107","type":"journal-article","article-title":"Biologics: The role of delivery systems in improved therapy","volume":"8","author":"\u0160kalko-Basnet","year":"2014","unstructured":"\u0160kalko-Basnet, N. Biologics: The role of delivery systems in improved therapy. Biologics. 8, 107-114 (2014).","journal-title":"Biologics"},{"issue":"12","key":"ref21","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1177\/0960327115603594","type":"journal-article","article-title":"A snapshot of biologic drug development: Challenges and opportunities","volume":"34","author":"Andrews","year":"2015","unstructured":"Andrews, L., Ralston, S., Blomme, E., Barnhart, K. A snapshot of biologic drug development: Challenges and opportunities. Human Exp Toxicol. 34 (12), 1279-1285 (2015).","journal-title":"Human Exp Toxicol"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1038\/nrd3974","type":"journal-article","article-title":"Challenges and approaches for the development of safer immunomodulatory biologics","volume":"12","author":"Sathish","year":"2013","unstructured":"Sathish, J. G. et al. Challenges and approaches for the development of safer immunomodulatory biologics. Nat Rev Drug Disc. 12 (4), 306-324 (2013).","journal-title":"Nat Rev Drug Disc"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jconrel.2014.02.002","type":"journal-article","article-title":"Process for protein PEGylation","volume":"180","author":"Pfister","year":"2014","unstructured":"Pfister, D., Morbidelli, M. Process for protein PEGylation. J Control Rel. 180, 134-149 (2014).","journal-title":"J Control Rel"},{"issue":"9","key":"ref24","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1038\/nbt.3330","type":"journal-article","article-title":"Principles of nanoparticle design for overcoming biological barriers to drug delivery","volume":"33","author":"Blanco","year":"2015","unstructured":"Blanco, E., Shen, H., Ferrari, M. Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat Biotechnol. 33 (9), 941-951 (2015).","journal-title":"Nat Biotechnol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.addr.2018.07.007","type":"journal-article","article-title":"Tumor targeting via EPR: Strategies to enhance patient responses","volume":"130","author":"Golombek","year":"2018","unstructured":"Golombek, S. K. et al. Tumor targeting via EPR: Strategies to enhance patient responses. Adv Drug Del Rev. 130, 17-38 (2018).","journal-title":"Adv Drug Del Rev"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1021\/acs.biomac.0c01238","type":"journal-article","article-title":"Simultaneous cross-linking and cross-polymerization of enzyme responsive polyethylene glycol nanogels in confined aqueous droplets for reduction of low-density lipoprotein oxidation","volume":"22","author":"Basak","year":"2021","unstructured":"Basak, S. et al. Simultaneous cross-linking and cross-polymerization of enzyme responsive polyethylene glycol nanogels in confined aqueous droplets for reduction of low-density lipoprotein oxidation. Biomacromol. 22 (2), 386-398 (2021).","journal-title":"Biomacromol"},{"issue":"5","key":"ref27","doi-asserted-by":"crossref","first-page":"4256","DOI":"10.1021\/acsanm.1c00170","type":"journal-article","article-title":"Meta-analysis of in vitro drug-release parameters reveals predictable and robust kinetics for redox-responsive drug-conjugated therapeutic nanogels","volume":"4","author":"Morgulchik","year":"2021","unstructured":"Morgulchik, N., Kamaly, N. Meta-analysis of in vitro drug-release parameters reveals predictable and robust kinetics for redox-responsive drug-conjugated therapeutic nanogels. ACS Appl Nano Mat. 4 (5), 4256-4268 (2021).","journal-title":"ACS Appl Nano Mat"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1080\/1061186X.2018.1514041","type":"journal-article","article-title":"Redox-responsive smart nanogels for intracellular targeting of therapeutic agents: Applications and recent advances","volume":"27","author":"Ghorbani","year":"2019","unstructured":"Ghorbani, M., Hamishehkar, H. Redox-responsive smart nanogels for intracellular targeting of therapeutic agents: Applications and recent advances. J Drug Target. 27 (4), 408-422 (2019).","journal-title":"J Drug Target"},{"key":"ref29","first-page":"e5028181","type":"journal-article","article-title":"Glutathione &#34;redox homeostasis&#34; and its relation to cardiovascular disease","volume":"2019","author":"Bajic","year":"2019","unstructured":"Bajic, V. P. et al. Glutathione \"redox homeostasis\" and its relation to cardiovascular disease. Oxi Med Cell Long. 2019, e5028181 (2019).","journal-title":"Oxi Med Cell Long"},{"issue":"1-2","key":"ref30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2008.08.006","type":"journal-article","article-title":"Glutathione: Overview of its protective roles, measurement, and biosynthesis","volume":"30","author":"Forman","year":"2009","unstructured":"Forman, H. J., Zhang, H., Rinna, A. Glutathione: Overview of its protective roles, measurement, and biosynthesis. Mol Asp Med. 30 (1-2), 1-12 (2009).","journal-title":"Mol Asp Med"},{"issue":"13","key":"ref31","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1002\/marc.200900863","type":"journal-article","article-title":"Core-shell-corona micelle stabilized by reversible cross-linkage for intracellular drug delivery","volume":"31","author":"Wang","year":"2010","unstructured":"Wang, Y. C. et al. Core-shell-corona micelle stabilized by reversible cross-linkage for intracellular drug delivery. Macromol Rapid Comm. 31 (13), 1201-1206 (2010).","journal-title":"Macromol Rapid Comm"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1039\/C8BM01120F","type":"journal-article","article-title":"Degradable redox-responsive disulfide-based nanogel drug carriers via dithiol oxidation polymerization","volume":"7","author":"Elkassih","year":"2019","unstructured":"Elkassih, S. A., Kos, P., Xiong, H., Siegwart, D. J. Degradable redox-responsive disulfide-based nanogel drug carriers via dithiol oxidation polymerization. Biomat Sci. 7 (2), 607-617 (2019).","journal-title":"Biomat Sci"},{"issue":"3","key":"ref33","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1021\/acs.biomac.6b01704","type":"journal-article","article-title":"Stimuli-responsive polymersomes for biomedical applications","volume":"18","author":"Hu","year":"2017","unstructured":"Hu, X. et al. Stimuli-responsive polymersomes for biomedical applications. Biomacromol. 18 (3), 649-673 (2017).","journal-title":"Biomacromol"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.jconrel.2009.10.035","type":"journal-article","article-title":"Stimuli-responsive star poly(ethylene glycol) drug conjugates for improved intracellular delivery of the drug in neuroinflammation","volume":"142","author":"Navath","year":"2010","unstructured":"Navath, R. S., Wang, B., Kannan, S., Romero, R., Kannan, R. M. Stimuli-responsive star poly(ethylene glycol) drug conjugates for improved intracellular delivery of the drug in neuroinflammation. J Control Release. 142 (3), 447-456 (2010).","journal-title":"J Control Release"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1021\/acsnano.8b06400","type":"journal-article","article-title":"Glutathione-responsive prodrug nanoparticles for effective drug delivery and cancer therapy","volume":"13","author":"Ling","year":"2019","unstructured":"Ling, X. et al. Glutathione-responsive prodrug nanoparticles for effective drug delivery and cancer therapy. ACS Nano. 13 (1), 357-370 (2019).","journal-title":"ACS Nano"},{"issue":"14","key":"ref36","doi-asserted-by":"crossref","first-page":"2923","DOI":"10.1021\/jo00408a041","type":"journal-article","article-title":"Rapid chromatographic technique for preparative separations with moderate resolution","volume":"43","author":"Still","year":"1978","unstructured":"Still, W. C., Kahn, M., Mitra, A. Rapid chromatographic technique for preparative separations with moderate resolution. J Org Chem. 43 (14), 2923-2925 (1978).","journal-title":"J Org Chem"},{"key":"ref37","type":"journal-article","article-title":"JoVE science education database - Organic chemistry. Rotary evaporation to remove solvent","year":"2023","unstructured":"JoVE science education database - Organic chemistry. Rotary evaporation to remove solvent. JoVE, Cambridge, MA (2023).","journal-title":"JoVE"},{"key":"ref38","type":"journal-article","article-title":"JoVE science education database - Organic chemistry II. Infrared spectroscopy","year":"2024","unstructured":"JoVE science education database - Organic chemistry II. Infrared spectroscopy. JoVE, Cambridge, MA (2024).","journal-title":"JoVE"},{"key":"ref39","type":"journal-article","article-title":"science education database - Organic chemistry. Nuclear magnetic resonance (NMR) spectroscopy","year":"2023","unstructured":"JoVE science education database - Organic chemistry. Nuclear magnetic resonance (NMR) spectroscopy. JoVE, Cambridge, MA (2023).","journal-title":"JoVE"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"27","DOI":"10.5478\/MSL.2015.6.2.27","type":"journal-article","article-title":"Sample preparation for matrix-assisted laser desorption\/ionization mass spectrometry","volume":"6","author":"Kim","year":"2015","unstructured":"Kim, J. Sample preparation for matrix-assisted laser desorption\/ionization mass spectrometry. Mass Spec Lett. 6, 27-30 (2015).","journal-title":"Mass Spec Lett"},{"issue":"2","key":"ref41","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3390\/polym11020353","type":"journal-article","article-title":"Covalently crosslinked nanogels: An NMR study of the effect of monomer reactivity on composition and structure","volume":"11","author":"Liu","year":"2019","unstructured":"Liu, P., Pearce, C. M., Anastasiadi, R. M., Resmini, M., Castilla, A. M. Covalently crosslinked nanogels: An NMR study of the effect of monomer reactivity on composition and structure. Polymers. 11 (2), 353 (2019).","journal-title":"Polymers"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","type":"journal-article","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","unstructured":"Smith, P. K. et al. Measurement of protein using bicinchoninic acid. Anal Biochem. 150 (1), 76-85 (1985).","journal-title":"Anal Biochem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jconrel.2015.10.012","type":"journal-article","article-title":"Nanotechnology for protein delivery: Overview and perspectives","volume":"240","author":"Yu","year":"2016","unstructured":"Yu, M.;,Wu, J., Shi, J., Farokhzad, O. C. Nanotechnology for protein delivery: Overview and perspectives. J Control Release. 240, 24-37 (2016).","journal-title":"J Control Release"},{"issue":"4","key":"ref44","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1039\/D0PY01600D","type":"journal-article","article-title":"Fabrication of stimuli-responsive nanogels for protein encapsulation and traceless release without introducing organic solvents, surfactants, or small-molecule cross-linkers","volume":"12","author":"Zhang","year":"2021","unstructured":"Zhang, Y., Zhang, D., Wang, J. T., Zhang, X., Yang, Y. Fabrication of stimuli-responsive nanogels for protein encapsulation and traceless release without introducing organic solvents, surfactants, or small-molecule cross-linkers. Poly Chem. 12 (4), 554-563 (2021).","journal-title":"Poly Chem"},{"issue":"5","key":"ref45","doi-asserted-by":"crossref","first-page":"5280","DOI":"10.1021\/acsnano.6b01114","type":"journal-article","article-title":"Targeted interleukin-10 nanotherapeutics developed with a microfluidic chip enhance resolution of inflammation in advanced atherosclerosis","volume":"10","author":"Kamaly","year":"2016","unstructured":"Kamaly, N. et al. Targeted interleukin-10 nanotherapeutics developed with a microfluidic chip enhance resolution of inflammation in advanced atherosclerosis. ACS Nano. 10 (5), 5280-5292 (2016).","journal-title":"ACS Nano"},{"issue":"3","key":"ref46","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1039\/C4AN01495B","type":"journal-article","article-title":"Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors","volume":"140","author":"Lu","year":"2015","unstructured":"Lu, R. et al. Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors. Analyst. 140 (3), 765-770 (2015).","journal-title":"Analyst"},{"key":"ref47","first-page":"012016","type":"journal-article","article-title":"FTIR study of secondary structure of bovine serum albumin and ovalbumin","volume":"769","author":"Abrosimova","year":"2016","unstructured":"Abrosimova, K. V., Shulenina, O. V., Paston, S. V. FTIR study of secondary structure of bovine serum albumin and ovalbumin. J Phys: Conf Ser. 769, 012016 (2016).","journal-title":"J Phys: Conf Ser"},{"issue":"30","key":"ref48","doi-asserted-by":"crossref","first-page":"5418","DOI":"10.1002\/anie.200900441","type":"journal-article","article-title":"Nanogels as pharmaceutical carriers: Finite networks of infinite capabilities","volume":"48","author":"Kabanov","year":"2009","unstructured":"Kabanov, A. V., Vinogradov, S. V. Nanogels as pharmaceutical carriers: Finite networks of infinite capabilities. Ang Chem Int Ed Eng. 48 (30), 5418-5429 (2009).","journal-title":"Ang Chem Int Ed Eng"},{"issue":"1","key":"ref49","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1080\/10717544.2021.1955042","type":"journal-article","article-title":"Preparation and use of nanogels as carriers of drugs","volume":"28","author":"Li","year":"2021","unstructured":"Li, C., Obireddy, S. R., Lai, W.F. Preparation and use of nanogels as carriers of drugs. Drug Del. 28 (1), 1594-1602 (2021).","journal-title":"Drug Del"},{"issue":"6","key":"ref50","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1038\/s41565-021-00898-0","type":"journal-article","article-title":"The current landscape of nucleic acid therapeutics","volume":"16","author":"Kulkarni","year":"2021","unstructured":"Kulkarni, J. A. et al. The current landscape of nucleic acid therapeutics. Nat Nanotechnol. 16 (6), 630-643 (2021).","journal-title":"Nat Nanotechnol"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/app.jove.com\/t\/63981\/synthesis-stimuli-responsive-nanogels-using-aqueous-one-step","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:44:41Z","timestamp":1792597481000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/63981\/synthesis-stimuli-responsive-nanogels-using-aqueous-one-step"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,24]]},"references-count":50,"journal-issue":{"issue":"215","published-online":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.3791\/63981","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,24]]}}}