{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:39:47Z","timestamp":1792615187401,"version":"4.5.0"},"reference-count":12,"publisher":"MyJove Corporation","issue":"184","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/64069","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T20:00:35Z","timestamp":1654804835000},"source":"Crossref","is-referenced-by-count":3,"title":["Procurement and Decellularization of Rat Hindlimbs Using an <em>Ex Vivo<\/em> Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation"],"prefix":"10.3791","author":[{"given":"Aisha","family":"Adil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Golnaz","family":"Karoubi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siba","family":"Haykal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1111\/tri.12652","type":"journal-article","article-title":"Vascularized composite allotransplantation: Current standards and novel approaches to prevent acute rejection and chronic allograft deterioration","volume":"29","author":"Kueckelhaus","year":"2016","unstructured":"Kueckelhaus, M. et al. Vascularized composite allotransplantation: Current standards and novel approaches to prevent acute rejection and chronic allograft deterioration. Transplant International. 29 (6), 655-662 (2016).","journal-title":"Transplant International"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1097\/SLA.0000000000002396","type":"journal-article","article-title":"Bioengineering a human face graft: The matrix of identity","volume":"266","author":"Duisit","year":"2017","unstructured":"Duisit, J. et al. Bioengineering a human face graft: The matrix of identity. Annals of Surgery. 266 (5), 754-764 (2017).","journal-title":"Annals of Surgery"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.actbio.2016.02.017","type":"journal-article","article-title":"Decellularized skin\/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps","volume":"35","author":"Zhang","year":"2016","unstructured":"Zhang, Q. et al. Decellularized skin\/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps. Acta Biomaterialia. 35, 166-184 (2016).","journal-title":"Acta Biomaterialia"},{"issue":"10","key":"ref4","first-page":"1","type":"journal-article","article-title":"Emerging implications for extracellular matrix-based technologies in vascularized composite allotransplantation","volume":"2016","author":"Londono","year":"2016","unstructured":"Londono, R., Gorantla, V. S., Badylak, S. F. Emerging implications for extracellular matrix-based technologies in vascularized composite allotransplantation. Stem Cells International. 2016 (10), 1-16 (2016).","journal-title":"Stem Cells International"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"205031212096871","DOI":"10.1177\/2050312120968721","type":"journal-article","article-title":"Evolution of the rat hind limb transplant as an experimental model of vascularized composite allotransplantation: Approaches and advantages","volume":"8","author":"Fleissig","year":"2020","unstructured":"Fleissig, Y. Y., Beare, J. E., LeBlanc, A. J., Kaufman, C. L. Evolution of the rat hind limb transplant as an experimental model of vascularized composite allotransplantation: Approaches and advantages. SAGE Open Medicine. 8, 205031212096871 (2020).","journal-title":"SAGE Open Medicine"},{"issue":"9","key":"ref6","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1016\/j.bioactmat.2021.02.010","type":"journal-article","article-title":"Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal","volume":"6","author":"Tao","year":"2021","unstructured":"Tao, M. et al. Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal. Bioactive Materials. 6 (9), 2927-2945 (2021).","journal-title":"Bioactive Materials"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/7651_2017_56","type":"journal-article","article-title":"Preparation of decellularized liver scaffolds and recellularized liver grafts","volume":"1577","author":"Chen","year":"2018","unstructured":"Chen, Y., Geerts, S., Jaramillo, M., Uygun, B. E. Preparation of decellularized liver scaffolds and recellularized liver grafts. Methods in Molecular Biology. 1577, 255-270 (2018).","journal-title":"Methods in Molecular Biology"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10529-018-2611-7","type":"journal-article","article-title":"New generation of bioreactors that advance extracellular matrix modelling and tissue engineering","volume":"41","author":"Ahmed","year":"2019","unstructured":"Ahmed, S., Chauhan, V. M., Ghaemmaghami, A. M., Aylott, J. W. New generation of bioreactors that advance extracellular matrix modelling and tissue engineering. Biotechnology Letters. 41 (1), 1-25 (2019).","journal-title":"Biotechnology Letters"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"13437","DOI":"10.1038\/s41598-021-92823-7","type":"journal-article","article-title":"Generation of vascular chimerism within donor organs","volume":"11","author":"Cohen","year":"2021","unstructured":"Cohen, S. et al. Generation of vascular chimerism within donor organs. Scientific Reports. 11 (1), 13437 (2021).","journal-title":"Scientific Reports"},{"key":"ref10","type":"journal-article","article-title":"Engineering vascularized composite allografts using natural scaffolds: A systematic review","author":"Lupon","year":"2021","unstructured":"Lupon, E. et al. Engineering vascularized composite allografts using natural scaffolds: A systematic review. Tissue Engineering Part B: Reviews. (2021).","journal-title":"Tissue Engineering Part B: Reviews"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"8398","DOI":"10.1038\/s41598-018-26371-y","type":"journal-article","article-title":"Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration","volume":"8","author":"Urciuolo","year":"2018","unstructured":"Urciuolo, A. et al. Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration. Scientific Reports. 8 (1), 8398 (2018).","journal-title":"Scientific Reports"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.biomaterials.2015.04.051","type":"journal-article","article-title":"Engineered composite tissue as a bioartificial limb graft","volume":"61","author":"Jank","year":"2015","unstructured":"Jank, B. J. et al. Engineered composite tissue as a bioartificial limb graft. Biomaterials. 61, 246-256 (2015).","journal-title":"Biomaterials"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/64069\/procurement-decellularization-rat-hindlimbs-using-an-ex-vivo","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:48:04Z","timestamp":1792612084000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/64069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,9]]},"references-count":12,"journal-issue":{"issue":"184","published-online":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.3791\/64069","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,9]]}}}