{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:39:05Z","timestamp":1792611545673,"version":"4.5.0"},"reference-count":30,"publisher":"MyJove Corporation","issue":"192","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/64718","type":"journal-article","created":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T19:02:15Z","timestamp":1676055735000},"source":"Crossref","is-referenced-by-count":2,"title":["<em>In Vivo<\/em> Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection"],"prefix":"10.3791","author":[{"given":"Wen","family":"Bu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2023,2,10]]},"reference":[{"key":"ref1","type":"book","volume-title":"Methods in Mammary Gland Biology and Breast Cancer Research. Eds Margot","author":"Nguyen","year":"2000","unstructured":"Nguyen, D.-A., Beeman, N., Lewis, M., Schaack, J., Neville, M. C. in Methods in Mammary Gland Biology and Breast Cancer Research. Eds Margot M. Ip, Bonnie B. Asch. Springer US, 259-270 (2000)."},{"issue":"4526","key":"ref2","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1126\/science.6272397","type":"journal-article","article-title":"Integration and stable germ line transmission of genes injected into mouse pronuclei","volume":"214","author":"Gordon","year":"1981","unstructured":"Gordon, J. W., Ruddle, F. H. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science. 214 (4526), 1244-1246 (1981).","journal-title":"Science"},{"issue":"5836","key":"ref3","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/294092a0","type":"journal-article","article-title":"Introduction of a rabbit beta-globin gene into the mouse germ line","volume":"294","author":"Costantini","year":"1981","unstructured":"Costantini, F., Lacy, E. Introduction of a rabbit beta-globin gene into the mouse germ line. Nature. 294 (5836), 92-94 (1981).","journal-title":"Nature"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0092-8674(81)90376-7","type":"journal-article","article-title":"Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs","volume":"27","author":"Brinster","year":"1981","unstructured":"Brinster, R. L. et al. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell. 27 (1 Pt 2), 223-231 (1981).","journal-title":"Cell"},{"issue":"7","key":"ref5","doi-asserted-by":"crossref","first-page":"823","DOI":"10.4161\/cc.6.7.4074","type":"journal-article","article-title":"RCAS-TVA in the mammary gland: an in vivo oncogene screen and a high fidelity model for breast transformation","volume":"6","author":"Du","year":"2007","unstructured":"Du, Z., Li, Y. RCAS-TVA in the mammary gland: an in vivo oncogene screen and a high fidelity model for breast transformation? Cell Cycle. 6 (7), 823-826 (2007).","journal-title":"Cell Cycle"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1158\/0008-5472.CAN-18-1087","type":"journal-article","article-title":"Mammary precancerous stem and non-stem cells evolve into cancers of distinct subtypes","volume":"79","author":"Bu","year":"2019","unstructured":"Bu, W. et al. Mammary precancerous stem and non-stem cells evolve into cancers of distinct subtypes. Cancer Research. 79 (1), 61-71 (2019).","journal-title":"Cancer Research"},{"issue":"43","key":"ref7","doi-asserted-by":"crossref","first-page":"4399","DOI":"10.1038\/onc.2011.147","type":"journal-article","article-title":"Keratin 6a marks mammary bipotential progenitor cells that can give rise to a unique tumor model resembling human normal-like breast cancer","volume":"30","author":"Bu","year":"2011","unstructured":"Bu, W. et al. Keratin 6a marks mammary bipotential progenitor cells that can give rise to a unique tumor model resembling human normal-like breast cancer. Oncogene. 30 (43), 4399-4409 (2011).","journal-title":"Oncogene"},{"issue":"50","key":"ref8","doi-asserted-by":"crossref","first-page":"5729","DOI":"10.1038\/onc.2013.521","type":"journal-article","article-title":"Contribution of an alveolar cell of origin to the high-grade malignant phenotype of pregnancy-associated breast cancer","volume":"33","author":"Haricharan","year":"2014","unstructured":"Haricharan, S. et al. Contribution of an alveolar cell of origin to the high-grade malignant phenotype of pregnancy-associated breast cancer. Oncogene. 33 (50), 5729-5739 (2014).","journal-title":"Oncogene"},{"issue":"11","key":"ref9","doi-asserted-by":"crossref","first-page":"78720","DOI":"10.1371\/journal.pone.0078720","type":"journal-article","article-title":"Mammary cells with active Wnt signaling resist ErbB2-induced tumorigenesis","volume":"8","author":"Bu","year":"2013","unstructured":"Bu, W., Zhang, X., Dai, H., Huang, S., Li, Y. Mammary cells with active Wnt signaling resist ErbB2-induced tumorigenesis. PLoS One. 8 (11), e78720 (2013).","journal-title":"PLoS One"},{"issue":"46","key":"ref10","doi-asserted-by":"crossref","first-page":"17396","DOI":"10.1073\/pnas.0608607103","type":"journal-article","article-title":"Introduction of oncogenes into mammary glands in vivo with an avian retroviral vector initiates and promotes carcinogenesis in mouse models","volume":"103","author":"Du","year":"2006","unstructured":"Du, Z. et al. Introduction of oncogenes into mammary glands in vivo with an avian retroviral vector initiates and promotes carcinogenesis in mouse models. Proceedings of the National Academy of Sciences of the United States of America. 103 (46), 17396-17401 (2006).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"7","key":"ref11","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1593\/neo.08266","type":"journal-article","article-title":"Lentivirus-mediated oncogene introduction into mammary cells in vivo induces tumors","volume":"10","author":"Siwko","year":"2008","unstructured":"Siwko, S. K. et al. Lentivirus-mediated oncogene introduction into mammary cells in vivo induces tumors. Neoplasia. 10 (7), 653-662 (2008).","journal-title":"Neoplasia"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s10911-009-9154-4","type":"journal-article","article-title":"Lentivirus vectors for stably introducing genes into mammary epithelial cells in vivo","volume":"14","author":"Bu","year":"2009","unstructured":"Bu, W., Xin, L., Toneff, M., Li, L., Li, Y. Lentivirus vectors for stably introducing genes into mammary epithelial cells in vivo. Journal of Mammary Gland Biology and Neoplasia. 14 (4), 401-404 (2009).","journal-title":"Journal of Mammary Gland Biology and Neoplasia"},{"issue":"10","key":"ref13","doi-asserted-by":"crossref","first-page":"5801","DOI":"10.1128\/JVI.77.10.5801-5809.2003","type":"journal-article","article-title":"Transduction of the mammary epithelium with adenovirus vectors in vivo","volume":"77","author":"Russell","year":"2003","unstructured":"Russell, T. D. et al. Transduction of the mammary epithelium with adenovirus vectors in vivo. Journal of Virology. 77 (10), 5801-5809 (2003).","journal-title":"Journal of Virology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"15115","DOI":"10.1038\/srep15115","type":"journal-article","article-title":"Adeno-associated-virus-mediated transduction of the mammary gland enables sustained production of recombinant proteins in milk","volume":"5","author":"Wagner","year":"2015","unstructured":"Wagner, S., Thresher, R., Bland, R., Laible, G. Adeno-associated-virus-mediated transduction of the mammary gland enables sustained production of recombinant proteins in milk. Scientific Reports. 5, 15115 (2015).","journal-title":"Scientific Reports"},{"issue":"5259","key":"ref15","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1126\/science.272.5259.263","type":"journal-article","article-title":"In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector","volume":"272","author":"Naldini","year":"1996","unstructured":"Naldini, L. et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science. 272 (5259), 263-267 (1996).","journal-title":"Science"},{"key":"ref16","type":"book","volume-title":"Retroviruses","author":"Coffin","year":"1997","unstructured":"Coffin, J. M., Hughes, S. H., Varmus, H. E. Retroviruses. Cold Spring Harbor Laboratory Press. (1997)."},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"135","DOI":"10.2174\/1566523024605591","type":"journal-article","article-title":"Adenovirus as an integrating vector","volume":"2","author":"Mitani","year":"2002","unstructured":"Mitani, K., Kubo, S. Adenovirus as an integrating vector. Current Gene Therapy. 2 (2), 135-144 (2002).","journal-title":"Current Gene Therapy"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1146\/annurev.genet.37.110801.143717","type":"journal-article","article-title":"Integration of adeno-associated virus (AAV) and recombinant AAV vectors","volume":"38","author":"McCarty","year":"2004","unstructured":"McCarty, D. M., Young, S. M., Jr., Samulski, R. J. Integration of adeno-associated virus (AAV) and recombinant AAV vectors. Annual Review of Genetics. 38, 819-845 (2004).","journal-title":"Annual Review of Genetics"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s10911-020-09469-w","type":"journal-article","article-title":"Intraductal injection of lentivirus vectors for stably introducing genes into rat mammary epithelial cells in vivo","volume":"25","author":"Bu","year":"2020","unstructured":"Bu, W., Li, Y. Intraductal injection of lentivirus vectors for stably introducing genes into rat mammary epithelial cells in vivo. Journal of Mammary Gland Biology and Neoplasia. 25 (4), 389-396 (2020).","journal-title":"Journal of Mammary Gland Biology and Neoplasia"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.ydbio.2010.07.027","type":"journal-article","article-title":"Genetic manipulation of individual somatic mammary cells in vivo reveals a master role of STAT5a in inducing alveolar fate commitment and lactogenesis even in the absence of ovarian hormones","volume":"346","author":"Dong","year":"2010","unstructured":"Dong, J. et al. Genetic manipulation of individual somatic mammary cells in vivo reveals a master role of STAT5a in inducing alveolar fate commitment and lactogenesis even in the absence of ovarian hormones. Developmental Biology. 346 (2), 196-203 (2010).","journal-title":"Developmental Biology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"00996","DOI":"10.7554\/eLife.00996","type":"journal-article","article-title":"Mechanism and preclinical prevention of increased breast cancer risk caused by pregnancy","volume":"2","author":"Haricharan","year":"2013","unstructured":"Haricharan, S. et al. Mechanism and preclinical prevention of increased breast cancer risk caused by pregnancy. eLife. 2, e00996 (2013).","journal-title":"eLife"},{"issue":"8","key":"ref22","doi-asserted-by":"crossref","first-page":"3728","DOI":"10.1073\/pnas.0910665107","type":"journal-article","article-title":"Defining the ATM-mediated barrier to tumorigenesis in somatic mammary cells following ErbB2 activation","volume":"107","author":"Reddy","year":"2010","unstructured":"Reddy, J. P. et al. Defining the ATM-mediated barrier to tumorigenesis in somatic mammary cells following ErbB2 activation. Proceedings of the National Academy of Sciences of the United States of America. 107 (8), 3728-3733 (2010).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"31","key":"ref23","doi-asserted-by":"crossref","first-page":"4149","DOI":"10.1038\/onc.2015.465","type":"journal-article","article-title":"The PR status of the originating cell of ER\/PR-negative mouse mammary tumors","volume":"35","author":"Dong","year":"2016","unstructured":"Dong, J. et al. The PR status of the originating cell of ER\/PR-negative mouse mammary tumors. Oncogene. 35 (31), 4149-4154 (2016).","journal-title":"Oncogene"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1158\/1940-6207.CAPR-21-0031","type":"journal-article","article-title":"Targeting the pro-survival protein BCL-2 to prevent breast cancer","volume":"15","author":"Young","year":"2022","unstructured":"Young, A. et al. Targeting the pro-survival protein BCL-2 to prevent breast cancer. Cancer Prevention Research. 15 (1), 3-10 (2022).","journal-title":"Cancer Prevention Research"},{"issue":"12","key":"ref25","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1097\/JOM.0000000000000879","type":"journal-article","article-title":"Risks associated with lentiviral vector exposures and prevention strategies","volume":"58","author":"Schlimgen","year":"2016","unstructured":"Schlimgen, R. et al. Risks associated with lentiviral vector exposures and prevention strategies. Journal of Occupational and Environmental Medicine. 58 (12), 1159-1166 (2016).","journal-title":"Journal of Occupational and Environmental Medicine"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"0117239","DOI":"10.1371\/journal.pone.0117239","type":"journal-article","article-title":"Krt6a-positive mammary epithelial progenitors are not at increased vulnerability to tumorigenesis initiated by ErbB2","volume":"10","author":"Holloway","year":"2015","unstructured":"Holloway, K. R. et al. Krt6a-positive mammary epithelial progenitors are not at increased vulnerability to tumorigenesis initiated by ErbB2. PLoS One. 10 (1), e0117239 (2015).","journal-title":"PLoS One"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"381","DOI":"10.7150\/ijbs.12947","type":"journal-article","article-title":"Targeting oncogenes into a defined subset of mammary cells demonstrates that the initiating oncogenic mutation defines the resulting tumor phenotype","volume":"12","author":"Holloway","year":"2016","unstructured":"Holloway, K. R. et al. Targeting oncogenes into a defined subset of mammary cells demonstrates that the initiating oncogenic mutation defines the resulting tumor phenotype. International Journal of Biological Sciences. 12 (4), 381-388 (2016).","journal-title":"International Journal of Biological Sciences"},{"issue":"11","key":"ref28","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1038\/onc.2015.206","type":"journal-article","article-title":"Luminal epithelial cells within the mammary gland can produce basal cells upon oncogenic stress","volume":"35","author":"Hein","year":"2015","unstructured":"Hein, S. M. et al. Luminal epithelial cells within the mammary gland can produce basal cells upon oncogenic stress. Oncogene. 35 (11), 1461-1467 (2015).","journal-title":"Oncogene"},{"issue":"5","key":"ref29","doi-asserted-by":"crossref","first-page":"102169","DOI":"10.15252\/embj.2019102169","type":"journal-article","article-title":"In situ CRISPR-Cas9 base editing for the development of genetically engineered mouse models of breast cancer","volume":"39","author":"Annunziato","year":"2020","unstructured":"Annunziato, S. et al. In situ CRISPR-Cas9 base editing for the development of genetically engineered mouse models of breast cancer. EMBO Journal. 39 (5), e102169 (2020).","journal-title":"EMBO Journal"},{"issue":"12","key":"ref30","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1101\/gad.279190.116","type":"journal-article","article-title":"Modeling invasive lobular breast carcinoma by CRISPR\/Cas9-mediated somatic genome editing of the mammary gland","volume":"30","author":"Annunziato","year":"2016","unstructured":"Annunziato, S. et al. Modeling invasive lobular breast carcinoma by CRISPR\/Cas9-mediated somatic genome editing of the mammary gland. Genes & Development. 30 (12), 1470-1480 (2016).","journal-title":"Genes & Development"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/64718\/in-vivo-gene-delivery-into-mouse-mammary-epithelial-cells-through","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:50:52Z","timestamp":1792608652000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/64718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,10]]},"references-count":30,"journal-issue":{"issue":"192","published-online":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.3791\/64718","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,10]]}}}