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Journal of Experimental Medicine. 179 (4), 1109-1118 (1994).","journal-title":"Journal of Experimental Medicine"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"369","DOI":"10.3389\/fimmu.2013.00369","type":"journal-article","article-title":"Regulation of MHC class II-peptide complex expression by ubiquitination","volume":"4","author":"Cho","year":"2013","unstructured":"Cho, K.-J., Roche, P. A. Regulation of MHC class II-peptide complex expression by ubiquitination. Frontiers in Immunology. 4, 369 (2013).","journal-title":"Frontiers in Immunology"},{"issue":"6","key":"ref41","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S1471-4906(03)00111-X","type":"journal-article","article-title":"What's the difference between CD80 and CD86","volume":"24","author":"Sansom","year":"2003","unstructured":"Sansom, D. M., Manzotti, C. N., Zheng, Y. What's the difference between CD80 and CD86? 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Journal of Experimental Medicine. 192 (3), 405-412 (2000).","journal-title":"Journal of Experimental Medicine"},{"issue":"10","key":"ref44","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1182\/blood-2010-01-264960","type":"journal-article","article-title":"Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR","volume":"116","author":"Klechevsky","year":"2010","unstructured":"Klechevsky, E. et al. Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR. Blood. 116 (10), 1685-1697 (2010).","journal-title":"Blood"},{"issue":"10","key":"ref45","doi-asserted-by":"crossref","first-page":"3490","DOI":"10.1182\/blood.V96.10.3490","type":"journal-article","article-title":"Efficient priming of protein antigen-specific human CD4+ T cells by monocyte-derived dendritic cells","volume":"96","author":"Schlienger","year":"2000","unstructured":"Schlienger, K., Craighead, N., Lee, K. P., Levine, B. L., June, C. H. 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Cold Spring Harbor Perspectives in Medicine. 1 (1), a007252 (2011).","journal-title":"Cold Spring Harbor Perspectives in Medicine"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"110092","DOI":"10.1016\/j.vetimm.2020.110092","type":"journal-article","article-title":"Development and evaluation of a real-time PCR panel for the detection of 20 immune markers in cattle and sheep","volume":"227","author":"Sassu","year":"2020","unstructured":"Sassu, E. L. et al. Development and evaluation of a real-time PCR panel for the detection of 20 immune markers in cattle and sheep. 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