{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:37:45Z","timestamp":1792611465085,"version":"4.5.0"},"reference-count":33,"publisher":"MyJove Corporation","issue":"193","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoVE"],"DOI":"10.3791\/64911","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T20:00:33Z","timestamp":1679688033000},"source":"Crossref","is-referenced-by-count":3,"title":["Production of Modified Autologous Conditioned Serum and &lt;em&gt;Ex Vivo&lt;\/em&gt; Assessment of Its Healing Potential in Murine Corneal Epithelium"],"prefix":"10.3791","author":[{"given":"Chun","family":"Hsiung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Ying","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Chuan","family":"Hsiung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Hsuan","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lung-Kun","family":"Yeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2098","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1002\/art.1780270415","type":"journal-article","article-title":"Beneficial effect of artificial tears made with autologous serum in patients with keratoconjunctivitis sicca","volume":"27","author":"Fox","year":"1984","unstructured":"Fox, R. I., Chan, R., Michelson, J. B., Belmont, J. B., Michelson, P. E. Beneficial effect of artificial tears made with autologous serum in patients with keratoconjunctivitis sicca. Arthritis and Rheumatology. 27 (4), 459-461 (1984).","journal-title":"Arthritis and Rheumatology"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1136\/bjo.2003.026211","type":"journal-article","article-title":"Comparison of autologous serum eye drops with conventional therapy in a randomised controlled crossover trial for ocular surface disease","volume":"88","author":"Noble","year":"2004","unstructured":"Noble, B. A. et al. Comparison of autologous serum eye drops with conventional therapy in a randomised controlled crossover trial for ocular surface disease. The British Journal of Ophthalmology. 88 (5), 647-652 (2004).","journal-title":"The British Journal of Ophthalmology"},{"issue":"8","key":"ref3","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1111\/j.1442-9071.2008.01895.x","type":"journal-article","article-title":"Serum growth factor analysis in dry eye syndrome","volume":"36","author":"Bradley","year":"2008","unstructured":"Bradley, J. C., Bradley, R. H., McCartney, D. L., Mannis, M. J. Serum growth factor analysis in dry eye syndrome. Clinical & Experimental Ophthalmology. 36 (8), 717-720 (2008).","journal-title":"Clinical & Experimental Ophthalmology"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.jsps.2015.01.023","type":"journal-article","article-title":"Comparison of different serum sample extraction methods and their suitability for mass spectrometry analysis","volume":"23","author":"Alshammari","year":"2015","unstructured":"Alshammari, T. M., Al-Hassan, A. A., Hadda, T. B., Aljofan, M. Comparison of different serum sample extraction methods and their suitability for mass spectrometry analysis. Saudi Pharmaceutical Journal. 23 (6), 689-697 (2015).","journal-title":"Saudi Pharmaceutical Journal"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1136\/bjo.83.4.390","type":"journal-article","article-title":"Treatment of dry eye by autologous serum application in Sj\u00f6gren's syndrome","volume":"83","author":"Tsubota","year":"1999","unstructured":"Tsubota, K. et al. Treatment of dry eye by autologous serum application in Sj\u00f6gren's syndrome. The British Journal of Ophthalmology. 83 (4), 390-395 (1999).","journal-title":"The British Journal of Ophthalmology"},{"issue":"8","key":"ref6","doi-asserted-by":"crossref","first-page":"684","DOI":"10.3109\/02713683.2012.674609","type":"journal-article","article-title":"Randomized double-blind clinical trial of autologous serum versus artificial tears in dry eye syndrome","volume":"37","author":"Urzua","year":"2012","unstructured":"Urzua, C. A., Vasquez, D. H., Huidobro, A., Hernandez, H., Alfaro, J. Randomized double-blind clinical trial of autologous serum versus artificial tears in dry eye syndrome. Current Eye Research. 37 (8), 684-688 (2012).","journal-title":"Current Eye Research"},{"issue":"5","key":"ref7","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1097\/ACI.0000000000000770","type":"journal-article","article-title":"Autologous serum eye drops for ocular surface disorders","volume":"21","author":"Cui","year":"2021","unstructured":"Cui, D., Li, G., Akpek, E. K. Autologous serum eye drops for ocular surface disorders. Current Opinion in Allergy and Clinical Immunology. 21 (5), 493-499 (2021).","journal-title":"Current Opinion in Allergy and Clinical Immunology"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3109\/02713683.2013.824987","type":"journal-article","article-title":"The application of autologous serum eye drops in severe dry eye patients; subjective and objective parameters before and after treatment","volume":"39","author":"Jirsova","year":"2014","unstructured":"Jirsova, K. et al. The application of autologous serum eye drops in severe dry eye patients; subjective and objective parameters before and after treatment. Current Eye Research. 39 (1), 21-30 (2014).","journal-title":"Current Eye Research"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","DOI":"10.1002\/14651858.CD009327.pub3","type":"journal-article","article-title":"Autologous serum eye drops for dry eye","volume":"2","author":"Pan","year":"2017","unstructured":"Pan, Q., Angelina, A., Marrone, M., Stark, W. J., Akpek, E. K. Autologous serum eye drops for dry eye. Cochrane Database of Systematic Reviews. 2 (2), CD009327 (2017).","journal-title":"Cochrane Database of Systematic Reviews"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1159\/000505630","type":"journal-article","article-title":"Autologous serum eye drops versus artificial tear drops for dry eye disease: a systematic review and meta-analysis of randomized controlled trials","volume":"63","author":"Wang","year":"2020","unstructured":"Wang, L. et al. Autologous serum eye drops versus artificial tear drops for dry eye disease: a systematic review and meta-analysis of randomized controlled trials. Ophthalmic Research. 63 (5), 443-451 (2020).","journal-title":"Ophthalmic Research"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1136\/bjophthalmol-2015-306842","type":"journal-article","article-title":"Blood-derived topical therapy for ocular surface diseases","volume":"100","author":"Soni","year":"2016","unstructured":"Soni, N. G., Jeng, B. H. Blood-derived topical therapy for ocular surface diseases. The British Journal of Ophthalmology. 100 (1), 22-27 (2016).","journal-title":"The British Journal of Ophthalmology"},{"issue":"10","key":"ref12","first-page":"2283","type":"journal-article","article-title":"anti-inflammatory forms of interleukin-1 in the tear fluid and conjunctiva of patients with dry-eye disease","volume":"42","author":"Solomon","year":"2001","unstructured":"Solomon, A. et al. Pro- and anti-inflammatory forms of interleukin-1 in the tear fluid and conjunctiva of patients with dry-eye disease. Investigative Ophthalmology and Visual Science. 42 (10), 2283-2292 (2001).","journal-title":"Investigative Ophthalmology and Visual Science"},{"issue":"10","key":"ref13","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1007\/s00011-003-1197-1","type":"journal-article","article-title":"The production of anti-inflammatory cytokines in whole blood by physico-chemical induction","volume":"52","author":"Meijer","year":"2003","unstructured":"Meijer, H., Reinecke, J., Becker, C., Tholen, G., Wehling, P. The production of anti-inflammatory cytokines in whole blood by physico-chemical induction. Inflammation Research. 52 (10), 404-407 (2003).","journal-title":"Inflammation Research"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1097\/ACI.0000000000000309","type":"journal-article","article-title":"Emerging therapeutics for ocular surface disease","volume":"16","author":"Bielory","year":"2016","unstructured":"Bielory, B. P., Shah, S. P., O'Brien, T. P., Perez, V. L., Bielory, L. Emerging therapeutics for ocular surface disease. Current Opinion in Allergy and Clinical Immunology. 16 (5), 477-486 (2016).","journal-title":"Current Opinion in Allergy and Clinical Immunology"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1001\/archophthalmol.2011.364","type":"journal-article","article-title":"Dry eye disease: an immune-mediated ocular surface disorder","volume":"130","author":"Stevenson","year":"2012","unstructured":"Stevenson, W., Chauhan, S. K., Dana, R. Dry eye disease: an immune-mediated ocular surface disorder. Archives of Ophthalmology. 130 (1), 90-100 (2012).","journal-title":"Archives of Ophthalmology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"101646","DOI":"10.1016\/j.tice.2021.101646","type":"journal-article","article-title":"Platelet-rich plasma attenuates interleukin-1\u03b2-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2\u03b1","volume":"73","author":"Yang","year":"2021","unstructured":"Yang, J., Guo, A., Li, Q., Wu, J. Platelet-rich plasma attenuates interleukin-1\u03b2-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2\u03b1. Tissue and Cell. 73, 101646 (2021).","journal-title":"Tissue and Cell"},{"issue":"1-6","key":"ref17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/08977194.2021.2012467","type":"journal-article","article-title":"Autologous conditioned serum for degenerative diseases and prospects","volume":"39","author":"Shakouri","year":"2021","unstructured":"Shakouri, S. K., Dolati, S., Santhakumar, J., Thakor, A. S., Yarani, R. Autologous conditioned serum for degenerative diseases and prospects. Growth Factors. 39 (1-6), 59-70 (2021).","journal-title":"Growth Factors"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"86","DOI":"10.3390\/catal11010086","type":"journal-article","article-title":"The role of glycerol and its derivatives in the biochemistry of living organisms, and their prebiotic origin and significance in the evolution of life","volume":"11","author":"Gull","year":"2021","unstructured":"Gull, M., Pasek, M. A. The role of glycerol and its derivatives in the biochemistry of living organisms, and their prebiotic origin and significance in the evolution of life. Catalysts. 11 (1), 86 (2021).","journal-title":"Catalysts"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3389\/fmed.2018.00033","type":"journal-article","article-title":"Reflections on dry eye syndrome treatment: therapeutic role of blood products","volume":"5","author":"Drew","year":"2018","unstructured":"Drew, V. J., Tseng, C. L., Seghatchian, J., Burnouf, T. Reflections on dry eye syndrome treatment: therapeutic role of blood products. Frontiers in Medicine. 5, 33 (2018).","journal-title":"Frontiers in Medicine"},{"issue":"182","key":"ref20","first-page":"e63217","type":"journal-article","article-title":"Ex vivo and in vivo animal models for mechanical and chemical injuries of corneal epithelium","author":"Hung","year":"2022","unstructured":"Hung, K. H., Yeh, L. K. Ex vivo and in vivo animal models for mechanical and chemical injuries of corneal epithelium. Journal of Visualized Experiments. (182), 63217 (2022).","journal-title":"Journal of Visualized Experiments"},{"issue":"11","key":"ref21","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1136\/bjo.2004.044347","type":"journal-article","article-title":"Autologous serum eye drops for ocular surface disorders","volume":"88","author":"Geerling","year":"2004","unstructured":"Geerling, G., Maclennan, S., Hartwig, D. Autologous serum eye drops for ocular surface disorders. The British Journal of Ophthalmology. 88 (11), 1467-1474 (2004).","journal-title":"The British Journal of Ophthalmology"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"R114","DOI":"10.1186\/ar3050","type":"journal-article","article-title":"Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection","volume":"12","author":"Rutgers","year":"2010","unstructured":"Rutgers, M., Saris, D. B., Dhert, W. J., Creemers, L. B. Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection. Arthritis Research & Therapy. 12 (3), R114 (2010).","journal-title":"Arthritis Research & Therapy"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1186\/s13287-018-1007-x","type":"journal-article","article-title":"Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cells","volume":"9","author":"Antebi","year":"2018","unstructured":"Antebi, B. et al. Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cells. Stem Cell Research & Therapy. 9 (1), 265 (2018).","journal-title":"Stem Cell Research & Therapy"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"6600788","DOI":"10.1155\/2022\/6600788","type":"journal-article","article-title":"Use of autologous serum eye drops with contact lenses in the treatment of chemical burn-induced bilateral corneal persistent epithelial defects","volume":"2022","author":"Chen","year":"2022","unstructured":"Chen, Y. M., Wang, W. Y., Lin, Y. C., Tsai, S. H., Lou, Y. T. Use of autologous serum eye drops with contact lenses in the treatment of chemical burn-induced bilateral corneal persistent epithelial defects. BioMed Research International. 2022, 6600788 (2022).","journal-title":"BioMed Research International"},{"issue":"4","key":"ref25","doi-asserted-by":"crossref","first-page":"e912","DOI":"10.1111\/aos.14997","type":"journal-article","article-title":"Comparison of the efficacy of topical insulin with autologous serum eye drops in persistent epithelial defects of the cornea","volume":"100","author":"Diaz-Valle","year":"2022","unstructured":"Diaz-Valle, D. et al. Comparison of the efficacy of topical insulin with autologous serum eye drops in persistent epithelial defects of the cornea. Acta Ophthalmologica. 100 (4), e912-e919 (2022).","journal-title":"Acta Ophthalmologica"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"8906","DOI":"10.1038\/s41598-022-12879-x","type":"journal-article","article-title":"Comparison of epitheliotrophic factors in platelet-rich plasma versus autologous serum and their treatment efficacy in dry eye disease","volume":"12","author":"Metheetrairut","year":"2022","unstructured":"Metheetrairut, C. et al. Comparison of epitheliotrophic factors in platelet-rich plasma versus autologous serum and their treatment efficacy in dry eye disease. Scientific Reports. 12 (1), 8906 (2022).","journal-title":"Scientific Reports"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1080\/07853890.2022.2045035","type":"journal-article","article-title":"Neurotrophic keratopathy: current challenges and future prospects","volume":"54","author":"NaPier","year":"2022","unstructured":"NaPier, E., Camacho, M., McDevitt, T. F., Sweeney, A. R. Neurotrophic keratopathy: current challenges and future prospects. Annals of Medicine. 54 (1), 666-673 (2022).","journal-title":"Annals of Medicine"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"e170","DOI":"10.1111\/aos.13907","type":"journal-article","article-title":"Efficacy and safety of treatment of hyposecretory dry eye with platelet-rich plasma","volume":"97","author":"Garcia-Conca","year":"2019","unstructured":"Garcia-Conca, V. et al. Efficacy and safety of treatment of hyposecretory dry eye with platelet-rich plasma. Acta Ophthalmologica. 97 (2), e170-e178 (2019).","journal-title":"Acta Ophthalmologica"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"68","DOI":"10.12968\/jowc.2022.31.1.68","type":"journal-article","article-title":"Use of autologous conditioned serum dressings in hard-to-heal wounds: a randomised prospective clinical trial","volume":"31","author":"Gholian","year":"2022","unstructured":"Gholian, S. et al. Use of autologous conditioned serum dressings in hard-to-heal wounds: a randomised prospective clinical trial. Journal of Wound Care. 31 (1), 68-77 (2022).","journal-title":"Journal of Wound Care"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"776","DOI":"10.2144\/fsoa-2021-0088","type":"journal-article","article-title":"Autologous conditioned serum applications in the treatment of musculoskeletal diseases: a narrative review","volume":"8","author":"Raeissadat","year":"2022","unstructured":"Raeissadat, S. A., Rayegani, S. M., Jafarian, N., Heidari, M. Autologous conditioned serum applications in the treatment of musculoskeletal diseases: a narrative review. Future Science OA. 8 (2), FSO776 (2022).","journal-title":"Future Science OA"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.rvsc.2022.03.009","type":"journal-article","article-title":"Autologous conditioned serum in equine and human orthopedic therapy: A systematic review","volume":"146","author":"Tokawa","year":"2022","unstructured":"Tokawa, P. K. A., Brossi, P. M., Baccarin, R. Y. A. Autologous conditioned serum in equine and human orthopedic therapy: A systematic review. Research in Veterinary Science. 146, 34-52 (2022).","journal-title":"Research in Veterinary Science"},{"issue":"4","key":"ref32","first-page":"893","type":"journal-article","article-title":"Autologous conditioned serum. Physical Medicine and Rehabilitation","volume":"27","author":"Evans","year":"2016","unstructured":"Evans, C. H., Chevalier, X., Wehling, P. Autologous conditioned serum. Physical Medicine and Rehabilitation Clinics of North America. 27 (4), 893-908 (2016).","journal-title":"Clinics of North America"},{"issue":"4","key":"ref33","first-page":"e24500","type":"journal-article","article-title":"Platelet rich plasma versus autologous conditioned serum in osteoarthritis of the knee: clinical results of a five-year retrospective study","volume":"14","author":"Coskun","year":"2022","unstructured":"Coskun, H. S., Yurtbay, A., Say, F. Platelet rich plasma versus autologous conditioned serum in osteoarthritis of the knee: clinical results of a five-year retrospective study. Cureus. 14 (4), e24500 (2022).","journal-title":"Cureus"}],"container-title":["Journal of Visualized Experiments"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jove.com\/t\/64911\/production-modified-autologous-conditioned-serum-ex-vivo-assessment","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:38:01Z","timestamp":1792607881000},"score":1,"resource":{"primary":{"URL":"https:\/\/app.jove.com\/t\/64911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,24]]},"references-count":33,"journal-issue":{"issue":"193","published-online":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.3791\/64911","relation":{},"ISSN":["1940-087X"],"issn-type":[{"value":"1940-087X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,24]]}}}